DE602004004988D1 - Methylierungsstatus-Detektionsassays mittels methylierungsspezifischer Primerextension (MSPE) - Google Patents

Methylierungsstatus-Detektionsassays mittels methylierungsspezifischer Primerextension (MSPE)

Info

Publication number
DE602004004988D1
DE602004004988D1 DE602004004988T DE602004004988T DE602004004988D1 DE 602004004988 D1 DE602004004988 D1 DE 602004004988D1 DE 602004004988 T DE602004004988 T DE 602004004988T DE 602004004988 T DE602004004988 T DE 602004004988T DE 602004004988 D1 DE602004004988 D1 DE 602004004988D1
Authority
DE
Germany
Prior art keywords
mspe
methylation
primer extension
specific primer
cpg sites
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004004988T
Other languages
English (en)
Other versions
DE602004004988T2 (de
Inventor
Zheng Li
Jeanne Harvey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Inc
Original Assignee
Bayer Healthcare LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Healthcare LLC filed Critical Bayer Healthcare LLC
Publication of DE602004004988D1 publication Critical patent/DE602004004988D1/de
Application granted granted Critical
Publication of DE602004004988T2 publication Critical patent/DE602004004988T2/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
DE602004004988T 2003-12-16 2004-12-16 Methylierungsstatus-Detektionsassays mittels methylierungsspezifischer Primerextension (MSPE) Active DE602004004988T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53001003P 2003-12-16 2003-12-16
US530010P 2003-12-16

Publications (2)

Publication Number Publication Date
DE602004004988D1 true DE602004004988D1 (de) 2007-04-12
DE602004004988T2 DE602004004988T2 (de) 2008-01-10

Family

ID=34520279

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004004988T Active DE602004004988T2 (de) 2003-12-16 2004-12-16 Methylierungsstatus-Detektionsassays mittels methylierungsspezifischer Primerextension (MSPE)

Country Status (6)

Country Link
US (2) US20050214812A1 (de)
EP (1) EP1544309B1 (de)
JP (1) JP2005204652A (de)
AT (1) ATE355390T1 (de)
DE (1) DE602004004988T2 (de)
ES (1) ES2281743T3 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007015993A1 (en) * 2005-07-26 2007-02-08 Siemens Healthcare Diagnostics Inc. Methylation specific primer extension assay for the detection of genomic imprinting disorders
CA2629008A1 (en) * 2005-11-08 2007-09-13 Euclid Diagnostics Llc Materials and methods for assaying for methylation of cpg islands associated with genes in the evaluation of cancer
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
WO2009059327A2 (en) * 2007-11-02 2009-05-07 University Of Southern California (Usc) Compositions and methods comprising biomarkers of sperm quality, semen quality and fertility
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
CA2746632C (en) * 2008-12-11 2020-06-30 Pacific Biosciences Of California, Inc. Characterization of modified nucleic acids
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
JP2013545439A (ja) 2010-09-17 2013-12-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 多能性幹細胞の有用性および安全性の特徴決定を行うための機能的ゲノミクスアッセイ
ES2688458T5 (es) * 2010-10-22 2022-04-13 Cold Spring Harbor Laboratory Recuento varietal de ácidos nucleicos para obtener información del número de copias genómicas
EP2694686B2 (de) 2011-04-06 2023-07-19 The University of Chicago Zusammensetzung und verfahren zur modifizierung von 5-methylcytosin (5mc)
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
WO2013163207A1 (en) 2012-04-24 2013-10-31 Pacific Biosciences Of California, Inc. Identification of 5-methyl-c in nucleic acid templates
US10174363B2 (en) * 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
WO2019028556A1 (en) * 2017-08-09 2019-02-14 Enrich Bioscience Inc. METHOD AND SYSTEM FOR ANALYZING DNA METHYLATION AND USE THEREOF FOR DETECTING CANCER
JP2021112127A (ja) * 2018-04-26 2021-08-05 暁生 黒田 膵β細胞の傷害検査方法
KR102468839B1 (ko) * 2020-06-03 2022-11-18 주식회사 제노헬릭스 Rna 검출 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
AU7829398A (en) * 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
US6297018B1 (en) * 1998-04-17 2001-10-02 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US7700324B1 (en) * 1998-11-03 2010-04-20 The Johns Hopkins University School Of Medicine Methylated CpG island amplification (MCA)
US6709816B1 (en) * 1999-10-18 2004-03-23 Affymetrix, Inc. Identification of alleles
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
DE10160983B4 (de) * 2001-12-05 2004-12-09 Epigenomics Ag Verfahren und Integrierte Vorrichtung zum Nachweis von Cytosinmethylierungen

Also Published As

Publication number Publication date
ES2281743T3 (es) 2007-10-01
US20080213789A1 (en) 2008-09-04
DE602004004988T2 (de) 2008-01-10
EP1544309A1 (de) 2005-06-22
ATE355390T1 (de) 2006-03-15
EP1544309B1 (de) 2007-02-28
US20050214812A1 (en) 2005-09-29
JP2005204652A (ja) 2005-08-04

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC. (N.D.GES.D, US

8328 Change in the person/name/address of the agent

Representative=s name: MAIER, D., DIPL.-ING. UNIV., PAT.-ASS., 81739 MUEN