HK1066570A1 - Method for assembly of a double-stranded polynucleotide - Google Patents

Method for assembly of a double-stranded polynucleotide

Info

Publication number
HK1066570A1
HK1066570A1 HK04109376.1A HK04109376A HK1066570A1 HK 1066570 A1 HK1066570 A1 HK 1066570A1 HK 04109376 A HK04109376 A HK 04109376A HK 1066570 A1 HK1066570 A1 HK 1066570A1
Authority
HK
Hong Kong
Prior art keywords
target
polynucleotide
model
double
assembly
Prior art date
Application number
HK04109376.1A
Other languages
English (en)
Inventor
Glen A Evans
Original Assignee
Centocor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centocor Inc filed Critical Centocor Inc
Publication of HK1066570A1 publication Critical patent/HK1066570A1/xx

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00511—Walls of reactor vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00691—Automatic using robots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00695—Synthesis control routines, e.g. using computer programs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
HK04109376.1A 2001-08-02 2004-11-27 Method for assembly of a double-stranded polynucleotide HK1066570A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/922,221 US6670127B2 (en) 1997-09-16 2001-08-02 Method for assembly of a polynucleotide encoding a target polypeptide
PCT/US2002/024488 WO2004033619A2 (en) 2001-08-02 2002-08-02 Method for assembly of a polynucleotide encoding a target polypeptide

Publications (1)

Publication Number Publication Date
HK1066570A1 true HK1066570A1 (en) 2005-03-24

Family

ID=32094305

Family Applications (1)

Application Number Title Priority Date Filing Date
HK04109376.1A HK1066570A1 (en) 2001-08-02 2004-11-27 Method for assembly of a double-stranded polynucleotide

Country Status (13)

Country Link
US (2) US6670127B2 (de)
EP (1) EP1470252B1 (de)
JP (1) JP2005519641A (de)
AT (1) ATE451473T1 (de)
AU (1) AU2002368136A1 (de)
CA (1) CA2454561A1 (de)
CY (1) CY1109845T1 (de)
DE (1) DE60234716D1 (de)
DK (1) DK1470252T3 (de)
ES (1) ES2336086T3 (de)
HK (1) HK1066570A1 (de)
PT (1) PT1470252E (de)
WO (1) WO2004033619A2 (de)

Families Citing this family (143)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
JP2004533228A (ja) * 2001-01-19 2004-11-04 エジー バイオサイエンシーズ， インコーポレイテッド 標的ポリペプチドをコードするポリヌクレオチドのコンピューターに従うアセンブリ
US20050079579A1 (en) * 2001-02-28 2005-04-14 Guangwen Wei Uses of spatial configuration to modulate protein function
US7164992B1 (en) * 2001-03-22 2007-01-16 Blue Heron Biotechnology, Inc. Method and system for polynucleotide synthesis
AU2003213696B2 (en) * 2002-02-28 2008-01-24 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US7482119B2 (en) * 2002-04-01 2009-01-27 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
AU2003218470A1 (en) * 2002-04-01 2003-10-20 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7871799B2 (en) * 2002-11-22 2011-01-18 Lawrence Livermore National Security, Llc Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US7932025B2 (en) * 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US7727969B2 (en) * 2003-06-06 2010-06-01 Massachusetts Institute Of Technology Controlled release nanoparticle having bound oligonucleotide for targeted delivery
WO2005037850A2 (en) * 2003-10-15 2005-04-28 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
US20050169816A1 (en) * 2003-12-15 2005-08-04 Kirshner Brian M. Automated oligomer synthesis
AU2005222788A1 (en) * 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
US20100016178A1 (en) * 2004-06-09 2010-01-21 Sussman Michael R Methods for rapid production of target double-stranded dna sequences
US7378259B2 (en) * 2004-07-15 2008-05-27 Applera Corporation Fluid processing device
US20070269870A1 (en) * 2004-10-18 2007-11-22 George Church Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
CA2594832A1 (en) * 2005-01-13 2006-07-20 Codon Devices, Inc. Compositions and methods for protein design
CN101133166B (zh) * 2005-03-10 2011-02-02 高晓莲 平行制备核酸及应用
WO2006121764A2 (en) * 2005-05-06 2006-11-16 Applera Corporation Multiple capillary device and method for synthesis and dispensing
WO2006127423A2 (en) * 2005-05-18 2006-11-30 Codon Devices, Inc. Methods of producing polynucleotide libraries using scarless ligation
AU2006202209B2 (en) * 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
DK1888123T3 (da) * 2005-06-08 2013-04-15 Janssen Biotech Inc Celleterapi til øjendegeneration
AU2006267039A1 (en) * 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for biocatalytic engineering
WO2007008951A1 (en) * 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for design of non-immunogenic proteins
EP1968994B1 (de) * 2005-12-06 2013-07-03 Synthetic Genomics, Inc. Synthetische genome
US20070148681A1 (en) * 2005-12-16 2007-06-28 Gene Oracle, Inc. Gene synthesis kit
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US20120156731A1 (en) * 2007-03-23 2012-06-21 The Board Of Trustees Of The Leland Stanford Junior University Improved Methods for Rapid Gene Synthesis
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
ES2614735T3 (es) 2007-07-23 2017-06-01 Janssen Biotech, Inc. Métodos y composiciones para tratar los trastornos relacionados con fibrosis usando antagonistas de la IL-17
CN101952415B (zh) 2007-07-31 2017-06-27 生命扫描有限公司 人胚胎干细胞的分化
CA2954431C (en) * 2007-11-27 2021-08-24 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
WO2009085462A1 (en) * 2007-12-19 2009-07-09 Centocor, Inc. Design and generation of human de novo pix phage display libraries via fusion to pix or pvii, vectors, antibodies and methods
US10066203B2 (en) 2008-02-21 2018-09-04 Janssen Biotech Inc. Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
EP2285974A2 (de) 2008-05-14 2011-02-23 British Columbia Cancer Agency Branch Gensynthese durch konvergentes zusammenfügen von oligonukleotid-bauteilsätzen
RU2533805C2 (ru) 2008-06-30 2014-11-20 Сентокор Орто Байотек Инк. Дифференцирование плюрипотентных стволовых клеток, представляющих собой линию клеток человека
US20100028307A1 (en) * 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
ES2727950T3 (es) 2008-10-31 2019-10-21 Janssen Biotech Inc Diferenciación de células madre embrionarias humanas en linaje endocrino pancreático
WO2010051223A1 (en) 2008-10-31 2010-05-06 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
AU2009316583B2 (en) * 2008-11-20 2016-04-21 Janssen Biotech, Inc. Methods and compositions for cell attachment and cultivation on planar substrates
WO2010059775A1 (en) 2008-11-20 2010-05-27 Centocor Ortho Biotech Inc. Pluripotent stem cell culture on micro-carriers
RU2579278C2 (ru) 2009-07-20 2016-04-10 Янссен Байотек, Инк. Популяция панкреатических эндокринных клеток-предшественников для снижения концентрации глюкозы в крови и способ дифференцировки панкреатических эндодермальных клеток
RU2540021C2 (ru) * 2009-07-20 2015-01-27 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека
CA2768720C (en) 2009-07-20 2018-12-18 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
DK2488867T3 (da) * 2009-10-14 2020-11-09 Janssen Biotech Inc Fremgangsmåder til affinitetsmodning af antistoffer
EP2311961A1 (de) * 2009-10-15 2011-04-20 DKFZ Deutsches Krebsforschungszentrum Modellgeführte Zufallszusammenlagerungs-Polymerase-Kettenreaktion für die Synthese von eukaryotischen Promotern
US20110104805A1 (en) * 2009-10-29 2011-05-05 Centocor Ortho Biotech Inc. Pluripotent Stem Cells
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US8728985B2 (en) 2009-11-17 2014-05-20 Centocor Ortho Biotech Inc. Display of disulfide linked dimeric proteins in filamentous phage
EP2501824A4 (de) 2009-11-17 2013-06-19 Janssen Biotech Inc Verbesserte proteinabscheidung auf bakterienmembranen
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
EP2504449B1 (de) 2009-11-25 2016-03-23 Gen9, Inc. Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion
CA2784425A1 (en) 2009-12-23 2011-06-30 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
MX343786B (es) 2009-12-23 2016-11-22 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas.
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2011109279A2 (en) * 2010-03-01 2011-09-09 Centocor Ortho Biotech Inc. Methods for purifying cells derived from pluripotent stem cells
US20110224086A1 (en) * 2010-03-09 2011-09-15 Jose Pardinas Methods and Algorithms for Selecting Polynucleotides For Synthetic Assembly
JP5908888B2 (ja) 2010-04-16 2016-04-26 ヤンセン バイオテツク，インコーポレーテツド 改変された植物のシステインプロテアーゼ及びその使用
HUE029622T2 (en) 2010-04-30 2017-03-28 Janssen Biotech Inc Stabilized fibronectin preparations, processes and applications
WO2011143299A2 (en) 2010-05-12 2011-11-17 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
PL2611909T3 (pl) 2010-08-31 2018-05-30 Janssen Biotech, Inc Zróżnicowanie ludzkich embrionalnych komórek macierzystych
PL2611907T3 (pl) 2010-08-31 2016-11-30 Różnicowanie pluripotencjalnych komórek macierzystych
US9528090B2 (en) 2010-08-31 2016-12-27 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP4039363A1 (de) 2010-11-12 2022-08-10 Gen9, Inc. Proteinanordnungen und verfahren zu ihrer herstellung und verwendung
EP2638157B1 (de) 2010-11-12 2015-07-22 Gen9, Inc. Verfahren und vorrichtungen für nukleinsäuresynthese
EP3594340B1 (de) 2011-08-26 2021-06-30 Gen9, Inc. Zusammensetzungen und verfahren zur hochqualitativen anordnung von nukleinsäuren
EA035150B1 (ru) 2011-09-27 2020-05-06 Янссен Байотек, Инк. Способ получения белковых каркасов на основе фибронектинового домена типа iii с альтернативными поверхностями связывания
CN105143446B (zh) 2011-12-22 2020-11-03 詹森生物科技公司 人胚胎干细胞分化成单一激素胰岛素阳性细胞
RU2664467C2 (ru) 2012-03-07 2018-08-17 Янссен Байотек, Инк. Среда определенного состава для размножения и обновления плюрипотентных стволовых клеток
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP3543350B1 (de) 2012-04-24 2021-11-10 Gen9, Inc. Verfahren zum sortieren von nukleinsäuren und zur multiplexierten, vorbereitenden in-vitro-klonung
US10414808B2 (en) 2012-05-18 2019-09-17 Janssen Biotech, Inc. Huwentoxin-IV variants and methods of use
US9102751B2 (en) 2012-05-18 2015-08-11 Janssen Biotech, Inc. Huwentoxin-IV variants and methods of use
WO2013173706A2 (en) 2012-05-18 2013-11-21 Janssen Biotech, Inc. Huwentoxin-iv variants and methods of use
MX355041B (es) 2012-05-25 2018-03-28 Janssen Biotech Inc Dominios de union a albumina consenso no naturales.
ES2690118T3 (es) 2012-06-08 2018-11-19 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas en células endocrinas pancreáticas
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
CA2896655C (en) 2012-12-31 2021-06-22 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
CN111394298A (zh) 2012-12-31 2020-07-10 詹森生物科技公司 使用hb9调节子使人胚胎干细胞分化为胰腺内分泌细胞的方法
US10377989B2 (en) 2012-12-31 2019-08-13 Janssen Biotech, Inc. Methods for suspension cultures of human pluripotent stem cells
TWI718086B (zh) 2013-01-07 2021-02-11 英屬維爾京群島商遠東超級實驗室有限公司 通過干擾素的經皮和/或經粘膜給藥治療骨癌、皮膚癌、皮下癌、粘膜癌和/或粘膜下癌的方法和組合物
RS57422B1 (sr) 2013-01-25 2018-09-28 Janssen Biotech Inc Antagonisti kv1.3 i postupci za upotrebu
GB2533173A (en) 2013-08-05 2016-06-15 Twist Bioscience Corp De Novo synthesized gene libraries
KR20160064192A (ko) 2013-10-03 2016-06-07 얀센 바이오테크 인코포레이티드 프로톡신-ii 변이체 및 사용 방법
BR112016026626A2 (pt) 2014-05-16 2017-08-15 Janssen Biotech Inc Uso de moléculas pequenas para melhorar a expressão de mafa em células pancreáticas endócrinas
US10487323B2 (en) * 2014-08-01 2019-11-26 The Regents Of The University Of California One-pot multiplex gene synthesis
US9850310B2 (en) 2014-09-05 2017-12-26 Janssen Pharmaceutica Nv CD123 binding agents and uses thereof
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
MA41642A (fr) 2015-03-03 2018-01-09 Janssen Biotech Inc Variants de protoxine ii et méthodes d'utilisation
MX2017012654A (es) 2015-04-02 2018-01-09 Janssen Biotech Inc Variantes de protoxina-ii y metodos de uso.
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10844122B2 (en) 2015-05-06 2020-11-24 Janssen Biotech, Inc. Prostate specific membrane antigen (PSMA) bispecific binding agents and uses thereof
ES2814550T3 (es) 2015-08-17 2021-03-29 Janssen Pharmaceutica Nv Anticuerpos anti-BCMA, moléculas que se unen a antígeno biespecífico que se unen a BCMA y CD3, y usos de los mismos
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
SG11201803675RA (en) 2015-11-02 2018-05-30 Janssen Pharmaceutica Nv Anti-il1rap antibodies, bispecific antigen binding molecules that bind il1rap and cd3, and uses thereof
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
TW201808987A (zh) 2016-06-08 2018-03-16 健生生物科技公司 Ｇｍ－ｃｓｆ變體及使用方法
SG11201901563UA (en) 2016-08-22 2019-03-28 Twist Bioscience Corp De novo synthesized nucleic acid libraries
EP3516528A4 (de) 2016-09-21 2020-06-24 Twist Bioscience Corporation Nukleinsäurebasierte datenspeicherung
EP3554561B1 (de) 2016-12-14 2023-06-28 Janssen Biotech, Inc. Cd137-bindende fibronectin-typ-iii-domänen
EP3932432A1 (de) 2016-12-14 2022-01-05 Janssen Biotech, Inc. Cd8a-bindende fibronectin-typ-iii-domänen
US10597438B2 (en) 2016-12-14 2020-03-24 Janssen Biotech, Inc. PD-L1 binding fibronectin type III domains
KR102514213B1 (ko) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 면역 시냅스의 변이체 라이브러리 및 그의 합성
JP2020508661A (ja) 2017-02-22 2020-03-26 ツイスト バイオサイエンス コーポレーション 核酸ベースのデータ保存
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
SG11202002194UA (en) 2017-09-11 2020-04-29 Twist Bioscience Corp Gpcr binding proteins and synthesis thereof
IL273700B1 (en) * 2017-10-13 2024-05-01 Ribbon Biolabs Gmbh A new method for the synthesis of polynucleotides by a diverse library of oligonucleotides
CA3079613A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
CN112041438A (zh) 2018-01-04 2020-12-04 特韦斯特生物科学公司 基于dna的数字信息存储
TW201940695A (zh) 2018-01-12 2019-10-16 英商卡美納生物科學公司 用於無模板幾何型酶催化性核酸合成之組成物和方法
EP3814497A4 (de) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung
JOP20200303A1 (ar) 2018-05-24 2020-11-23 Janssen Biotech Inc عوامل ربط psma واستخداماتها
MA52773A (fr) 2018-05-24 2021-04-14 Janssen Biotech Inc Anticorps anti-cd3 et leurs utilisations
WO2020041360A1 (en) 2018-08-21 2020-02-27 Quidel Corporation Dbpa antibodies and uses thereof
CN113766930A (zh) 2019-02-26 2021-12-07 特韦斯特生物科学公司 Glp1受体的变异核酸文库
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
BR112021017801A2 (pt) 2019-03-11 2022-01-18 Janssen Biotech Inc Anticorpos biespecíficos anti-vbeta17/anti-cd123
US20220145308A1 (en) * 2019-03-11 2022-05-12 University Of Florida Research Foundation, Inc. Materials and methods for reducing nucleic acid degradation in bacteria
WO2020208234A1 (en) 2019-04-10 2020-10-15 Ribbon Biolabs Gmbh A library of polynucleotides
MA55717A (fr) 2019-04-19 2022-02-23 Janssen Biotech Inc Méthodes de traitement du cancer de la prostate avec un anticorps anti-psma/cd3
JP2022550497A (ja) 2019-06-21 2022-12-02 ツイスト バイオサイエンス コーポレーション バーコードに基づいた核酸配列アセンブリ
WO2021076574A2 (en) 2019-10-14 2021-04-22 Aro Biotherapeutics Company Fn3 domain-sirna conjugates and uses thereof
WO2021076546A1 (en) 2019-10-14 2021-04-22 Aro Biotherapeutics Company Cd71 binding fibronectin type iii domains
US20210188971A1 (en) 2019-12-19 2021-06-24 Quidel Corporation Monoclonal antibody fusions
JP2023516084A (ja) * 2020-03-03 2023-04-17 コーデックス ディーエヌエー インコーポレイテッド 核酸をアセンブルするための方法
IL298046A (en) 2020-05-11 2023-01-01 Janssen Biotech Inc Treatment methods for multiple myeloma
JP2023540796A (ja) 2020-09-11 2023-09-26 ヤンセン バイオテツク，インコーポレーテツド ベータ鎖媒介性免疫を調節するための方法及び組成物
WO2023025488A1 (en) * 2021-08-23 2023-03-02 Dna Script Method for generating double-stranded nucleic acid

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4652639A (en) 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
EP0316018A3 (de) 1985-03-29 1989-07-26 Cetus Oncology Corporation Modifikation von DNS-Sequenzen
EP1186660A3 (de) 1985-03-30 2002-03-20 KAUFFMAN, Stuart A. Verfahren zum Erhalten von DNA, RNA, Peptiden, Polypeptiden oder Proteinen durch ein DNA-Rekombinations-Verfahren
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
AU3869289A (en) 1988-07-14 1990-02-05 Baylor College Of Medicine Solid phase assembly and reconstruction of biopolymers
US5262530A (en) 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5198346A (en) 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
ATE143696T1 (de) 1989-02-28 1996-10-15 Canon Kk Partiell doppelsträngiges oligonukleotid und verfahren zu seiner bildung
US5837528A (en) 1989-06-22 1998-11-17 Hoffmann La Roche, Inc. Bacterial strains which overproduce riboflavin
AU644619B2 (en) 1989-12-21 1993-12-16 Advanced Technologies (Cambridge) Limited Modification of plant metabolism
CA2079802C (en) * 1990-04-05 2001-09-18 Roberto Crea Walk-through mutagenesis
EP0550696B1 (de) 1990-09-28 2000-11-22 F. Hoffmann-La Roche Ag Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
EP0643775B1 (de) 1992-05-28 2004-07-21 Centre For Molecular Biology And Medicine, Quinone-derivate zur verbesserung der zellulare bioenergie
WO1994012632A1 (en) 1992-11-27 1994-06-09 University College London Improvements in nucleic acid synthesis by pcr
WO1994029442A2 (en) 1993-06-14 1994-12-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5591578A (en) 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US6110457A (en) 1995-12-08 2000-08-29 St. Louis University Live attenuated vaccines based on cp45 HPIV-3 strain and method to ensure attenuation in such vaccines
US6110668A (en) 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
US6159687A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
US6030771A (en) * 1997-08-25 2000-02-29 Centers For Disease Control And Prevention Mosaic protein and restriction endonuclease assisted ligation method for making the same
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
US6083726A (en) 1998-02-03 2000-07-04 Lucent Technologies, Inc. Methods for polynucleotide synthesis and articles for polynucleotide hybridization
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US7371851B1 (en) 1999-03-18 2008-05-13 Complete Genomics As Methods of cloning and producing fragment chains with readable information content
DE19925862A1 (de) * 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
US6274353B1 (en) 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
JP2004533228A (ja) * 2001-01-19 2004-11-04 エジー バイオサイエンシーズ， インコーポレイテッド 標的ポリペプチドをコードするポリヌクレオチドのコンピューターに従うアセンブリ

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