GB903346A - A gonadotropic hormone devoid of antigen properties - Google Patents
A gonadotropic hormone devoid of antigen propertiesInfo
- Publication number
- GB903346A GB903346A GB3525860A GB3525860A GB903346A GB 903346 A GB903346 A GB 903346A GB 3525860 A GB3525860 A GB 3525860A GB 3525860 A GB3525860 A GB 3525860A GB 903346 A GB903346 A GB 903346A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hormone
- precipitate
- washing
- ethanol
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 229940088597 hormone Drugs 0.000 title abstract 8
- 239000005556 hormone Substances 0.000 title abstract 8
- 239000000427 antigen Substances 0.000 title abstract 2
- 102000036639 antigens Human genes 0.000 title abstract 2
- 108091007433 antigens Proteins 0.000 title abstract 2
- 230000001456 gonadotroph Effects 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
- 238000005406 washing Methods 0.000 abstract 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
- 239000005995 Aluminium silicate Substances 0.000 abstract 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 abstract 2
- 239000005695 Ammonium acetate Substances 0.000 abstract 2
- 235000012211 aluminium silicate Nutrition 0.000 abstract 2
- 229940043376 ammonium acetate Drugs 0.000 abstract 2
- 235000019257 ammonium acetate Nutrition 0.000 abstract 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract 1
- 229910021536 Zeolite Inorganic materials 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 1
- 125000002091 cationic group Chemical group 0.000 abstract 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 1
- 230000003397 luteinic effect Effects 0.000 abstract 1
- 239000011707 mineral Substances 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 239000010457 zeolite Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
A gonadotropic hormone active both as a follicle-stimulant hormone, and as a luteinic hormone and devoid of antigen properties, is prepared by treating fresh urine obtained from women in menopausal or post-menopausal stage, at a pH of 4 to 4.5 with kaolin purified by washing with dilute mineral acid, separating the kaolin with adsorbed hormone, washing, extracting with aqueous ammonia of pH 11 to 11.3, centrifuging and adjusting the pH of the liquid so obtained to pH 8.5 by the addition of acetic acid, discarding the precipitate, lowering the pH of the residual liquid to pH 5 to 5.5 and adding acetone to precipitate the crude hormone which is further purified, repeated if necessary, by chromatographic adsorption on a cationic exchange zeolite, previously acidified to a pH of 3.7 to 3.8, followed by washing with water to neutrality, next with aqueous ethanol of 70 to 80% concentration, then with aqueous ethanol of 70 to 80% concentration containing ammonium acetate and finally eluting with aqueous ethanol of 35 to 45% concentration containing ammonium acetate, the hormone being precipitated from the eluate by increasing the ethanol content of the eluate, and is then washed and dried. It is preferred to precipitate the hormone by adjusting to an ethanol concentration of 80%.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT964060 | 1960-05-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB903346A true GB903346A (en) | 1962-08-15 |
Family
ID=11132851
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB3525860A Expired GB903346A (en) | 1960-05-31 | 1960-10-14 | A gonadotropic hormone devoid of antigen properties |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES262178A1 (en) |
| GB (1) | GB903346A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150072974A1 (en) * | 2012-05-09 | 2015-03-12 | Sunovion Pharmaceuticals Inc. | Heteroaryl compounds and methods of use thereof |
-
1960
- 1960-10-14 GB GB3525860A patent/GB903346A/en not_active Expired
- 1960-11-04 ES ES0262178A patent/ES262178A1/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150072974A1 (en) * | 2012-05-09 | 2015-03-12 | Sunovion Pharmaceuticals Inc. | Heteroaryl compounds and methods of use thereof |
| US9951088B2 (en) * | 2012-05-09 | 2018-04-24 | Sunovion Pharmaceuticals Inc. | D2 receptor modulators and methods of use thereof in the treatment of diseases and disorders |
Also Published As
| Publication number | Publication date |
|---|---|
| ES262178A1 (en) | 1961-01-01 |
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