GB644060A - Process of obtaining chorionic gonadotropic hormone - Google Patents
Process of obtaining chorionic gonadotropic hormoneInfo
- Publication number
- GB644060A GB644060A GB21722/47A GB2172247A GB644060A GB 644060 A GB644060 A GB 644060A GB 21722/47 A GB21722/47 A GB 21722/47A GB 2172247 A GB2172247 A GB 2172247A GB 644060 A GB644060 A GB 644060A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hormone
- per cent
- ethyl alcohol
- water
- impurities
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
Abstract
Chorionic gonadotropic hormone may be obtained from human pregnancy urine by adsorption of said hormone and associated impurities from said urine on to an ion exchange (e.g., zeolite) adsorbent and thereafter selectively desorbing and separating the hormone from the impurities, the desorbing steps being characterized by first desorbing mainly impurities from the absorbent by means of a substantially neutral mixture of water and a water-miscible organic solvent in concentrations of 60-95 per cent containing ammonium ions and thereafter treating the adsorbent with a substantially neutral mixture of water and a water-miscible organic solvent in concentrations of 10-50 per cent containing ammonium ions, whereby mainly hormone is removed from the adsorbent. The solvent may be acetone, dioxane or an aliphatic alcohol containing four or less carbon atoms of which ethyl alcohol is preferred. An ammonium salt of an organic carboxylic acid, particularly ammonium acetate, which salt is soluble in the particular solvent used, is the preferred source of ammonium ions. In the example, urine is clarified by centrifuging, acidified to a pH 3.5 by glacial acetic acid, reclarified and passed through a bed of synthetic zeolite for several days, the rate of flow being maintained by overnight water washings. Adsorbed impurities are removed by an aqueous wash liquor containing ammonium acetate and 75 per cent by volume of ethyl alcohol and the hormone is then obtained by eluting the column with a 40 per cent by volume ethyl alcohol liquor containing ammonium acetate and adjusting the ethyl alcohol strength of the eluate to 85 per cent by volume. The hormone may be further purified by washing separately with 95 per cent ethyl alcohol and acetone. The Specification as open to inspection under Sect. 91 states that solutions containing ammonium hydroxide or sodium ions may be used in the desorbing steps. This subject-matter does not appear in the Specification as accepted.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US644060XA | 1942-03-20 | 1942-03-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB644060A true GB644060A (en) | 1950-10-04 |
Family
ID=22055757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB21722/47A Expired GB644060A (en) | 1942-03-20 | 1947-08-07 | Process of obtaining chorionic gonadotropic hormone |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB644060A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2894875A (en) * | 1956-05-22 | 1959-07-14 | L Inst De Serotherapie Soc D | Method of producing the highly purified luteinizing gonadotropic factor |
-
1947
- 1947-08-07 GB GB21722/47A patent/GB644060A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2894875A (en) * | 1956-05-22 | 1959-07-14 | L Inst De Serotherapie Soc D | Method of producing the highly purified luteinizing gonadotropic factor |
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