GB837444A - Improvements in and relating to the preparation of elastase - Google Patents

Improvements in and relating to the preparation of elastase

Info

Publication number
GB837444A
GB837444A GB3278456A GB3278456A GB837444A GB 837444 A GB837444 A GB 837444A GB 3278456 A GB3278456 A GB 3278456A GB 3278456 A GB3278456 A GB 3278456A GB 837444 A GB837444 A GB 837444A
Authority
GB
United Kingdom
Prior art keywords
elastase
ionic strength
water
tissue
extract
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3278456A
Inventor
Norman Howard Grant
Kenneth Carl Robbins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Armour and Co
Original Assignee
Armour and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Armour and Co filed Critical Armour and Co
Priority to GB3278456A priority Critical patent/GB837444A/en
Publication of GB837444A publication Critical patent/GB837444A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6448—Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Elastase, an elastolytic enzyme, is prepared by extracting elastase-bearing tissue e.g. mammalian pancrease tissue of hog or beef to form an aqueous extract and contacting this extract, or pancreatic juice, with a weak cation exchanger especially a carboxylic acid resin at a pH of 4.5 to 5.0, and especially 4.7, and an ionic strength of not more than 0.4, and especially 0.05, to produce an adsorbate of the elastase and eluting therefrom with an aqueous solution, especially of an inert inorganic salt such as sodium chloride, having (a) a pH of 4.5 to 5.0 and especially 4.7 and an ionic strength of at least 0.4, and preferably 0.8 to 1.5; or (b) an ionic strength of at least 1.0. Trypsin or enterokinase is included in the crude aqueous concentrate of the enzyme prior to the adsorption step in order to activate the proelastase to elastase; alternatively the substance produced by elution from the cation exchanger is activated by treatment with trypsin. The elastase substance recovered from the eluate may be further purified by repeating the adsorption-elution process and then fractionating with (a) ammonium sulphate to saturation; or (b) alcohol especially methanol or ethanol of at least 65%, and especially 80%, by volume at a temperature of - 5 DEG C. The precipitate so produced is dialysed to remove salts and then lyophized. In an example, hog pancreas glands are subdivided, defatted with a solvent such as acetone, xylene or petroleum hydrocarbons, mixed with 10% porcine duodenal tissue and 8% sodium chloride and extracted with water at 25 DEG C. for 5 hours. The clarified extract is adsorbed on Amberlite XE-97 which has been equilibriated with acetate buffer to pH 4.7, the resin is washed with water and then eluted with acetate buffer of pH 4.7. Ammonium sulphate is added to the eluate, the elastase precipitate separated dissolved in water and lyophilized.
GB3278456A 1956-10-26 1956-10-26 Improvements in and relating to the preparation of elastase Expired GB837444A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB3278456A GB837444A (en) 1956-10-26 1956-10-26 Improvements in and relating to the preparation of elastase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3278456A GB837444A (en) 1956-10-26 1956-10-26 Improvements in and relating to the preparation of elastase

Publications (1)

Publication Number Publication Date
GB837444A true GB837444A (en) 1960-06-15

Family

ID=10343948

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3278456A Expired GB837444A (en) 1956-10-26 1956-10-26 Improvements in and relating to the preparation of elastase

Country Status (1)

Country Link
GB (1) GB837444A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1293705B (en) * 1963-10-10 1969-04-30 Rolland Lab A Process for obtaining an elastolytically effective pancreatic preparation
EP0057462A2 (en) * 1981-02-03 1982-08-11 Eisai Co., Ltd. Elastase-containing composition permitting elastase to be absorbed in increased amount
CN108642031A (en) * 2018-07-02 2018-10-12 四川德博尔制药有限公司 A kind of elastoser and its extracting method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1293705B (en) * 1963-10-10 1969-04-30 Rolland Lab A Process for obtaining an elastolytically effective pancreatic preparation
EP0057462A2 (en) * 1981-02-03 1982-08-11 Eisai Co., Ltd. Elastase-containing composition permitting elastase to be absorbed in increased amount
EP0057462A3 (en) * 1981-02-03 1982-12-29 Eisai Co., Ltd. Elastase-containing composition permitting elastase to be absorbed in increased amount
CN108642031A (en) * 2018-07-02 2018-10-12 四川德博尔制药有限公司 A kind of elastoser and its extracting method

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