GB894151A - Preparation of high purity streptokinase - Google Patents

Preparation of high purity streptokinase

Info

Publication number
GB894151A
GB894151A GB486/59A GB48659A GB894151A GB 894151 A GB894151 A GB 894151A GB 486/59 A GB486/59 A GB 486/59A GB 48659 A GB48659 A GB 48659A GB 894151 A GB894151 A GB 894151A
Authority
GB
United Kingdom
Prior art keywords
streptokinase
phosphate buffer
purified
treatment
column
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB486/59A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth Holdings LLC
Original Assignee
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Cyanamid Co filed Critical American Cyanamid Co
Publication of GB894151A publication Critical patent/GB894151A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3153—Streptokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention relates to a high purity streptokinase effective for the treatment of thrombosis which is an amorphous solid, soluble in water and having a molecular weight of about 47,000, a sedimentation rate constant of about 3.0 x 10-13, a diffusion constant of about 6.20 and 10-7 and an electrophoretic mobility (m ) of 4.19 x 10-5cm2sec-1volt-1, the preecding physical properties being measured in phosphate buffer at pH 7.5 and an ionic strength of 0.15. Crude streptokinase is adsorbed on an ionizable cellulose-type chromotographic column, desorbing purified streptokinase therefrom by means of gradient elution by means of a phosphate buffer having a molarity of 0.1 to 0.005 M, having a pH of 5.8 to 8.5 and a temperature of 0 DEG C. to 10 DEG C., recovering the streptokinase from the eluate by treatment with conventional precipitating agents for streptokinase, adsorbing said purified streptokinase on a second ionizable cellulose type chromatographic column, desorbing further purified streptokinase from said second column by means of gradient elution using the aforesaid phosphate buffer and recovering said further purified streptokinase from the eluate by conventional precipitating agents for streptokinase. Specifications 714,803, 715,103, 715,110, 715,572 and 715,930 are referred to.ALSO:An intravenous solution effective for the treatment of thrombosis comprises an aqueous solution of a purified streptokinase prepared by adsorption on a cellulose anion exchanger and elution with a phosphate buffer (see Group IV (b)). Preservatives, colouring-agents and other non-therapeutic injectable substances may be present. 50,000 to 100,000 units of streptokinase per hour may be administered.
GB486/59A 1958-01-29 1959-01-06 Preparation of high purity streptokinase Expired GB894151A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US894151XA 1958-01-29 1958-01-29

Publications (1)

Publication Number Publication Date
GB894151A true GB894151A (en) 1962-04-18

Family

ID=22217505

Family Applications (1)

Application Number Title Priority Date Filing Date
GB486/59A Expired GB894151A (en) 1958-01-29 1959-01-06 Preparation of high purity streptokinase

Country Status (1)

Country Link
GB (1) GB894151A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2121685A (en) * 1982-04-30 1984-01-04 Kaken Pharma Co Ltd Protease compositions for treating blood lipids abnormality and arteriosclerosis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2121685A (en) * 1982-04-30 1984-01-04 Kaken Pharma Co Ltd Protease compositions for treating blood lipids abnormality and arteriosclerosis

Similar Documents

Publication Publication Date Title
Carlsen et al. Purification and properties of an α-L-fucosidase from rat epididymis
Porath et al. Gel filtration of posterior pituitary hormones
Bouche The effect of spermidine on endonuclease inhibition by agarose contaminants
ES8407499A1 (en) Homogeneous human immune interferon and process therefor.
Bjork et al. Fractionation of snake venom by the gel-filtration method
GB1493427A (en) Method for recovery of refined alpha-galactosidase
EP0216936A4 (en) Novel tannin composition.
Mayhew et al. Chromatography of proteins on diethylaminoethyl-cellulose in concentrated ammonium sulfate
Miller Rivanol, resin and the isolation of thrombins
Rautela et al. Crystallization and partial characterization of Staphylococcus aureus hyaluronate lyase
Asai Chromatography of myosin
GB894151A (en) Preparation of high purity streptokinase
Berger et al. Application of HPLC to the purification of coupling factor CF1 from spinach chloroplasts and of some of its subunits
US2982696A (en) Ion-exchange procedures for the purification of penicillinase
US3184394A (en) Process for the preparation of enzymes, toxins and toxoids
US3107203A (en) Streptokinase purification process
US3905870A (en) Purification of kallikrein
US3597323A (en) Method of purifying l-asparaginase
ENDO Studies on Pectolytic Enzymes of Molds Part IX. Purification and Properties of Endo-Polygalacturonase II
Jutisz et al. Further evidence for the existence of several active components in sheep pituitary interstitial cell-stimulating hormone
CA1059937A (en) Isolation and purification of deoxyribonuclease
GB1507215A (en) Processes for producing immunoregulatory preparations
Davis et al. Electrophoretic characteristics of macroamylasemic serum
GB883549A (en) Improvements in and relating to the purification of antihaemophilic globulin
GB1419929A (en) Method for the preparation of high purity kallikrein