GB2554767A - Detecting and characterising a microorganism - Google Patents
Detecting and characterising a microorganism Download PDFInfo
- Publication number
- GB2554767A GB2554767A GB1617353.6A GB201617353A GB2554767A GB 2554767 A GB2554767 A GB 2554767A GB 201617353 A GB201617353 A GB 201617353A GB 2554767 A GB2554767 A GB 2554767A
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- culture vessel
- culture
- clinical sample
- microorganism
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Classifications
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- A—HUMAN NECESSITIES
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| US18/385,099 US20240368670A1 (en) | 2016-04-21 | 2023-10-30 | System for detecting and characterizing a microorganism |
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| US11053532B2 (en) | 2017-04-19 | 2021-07-06 | CAP Diagnostics, LLC | Methods for treating polymicrobial infections |
| EP3717657A1 (en) * | 2017-11-30 | 2020-10-07 | Ascilion AB | A system for assessing microbial susceptibility to antibiotic agents |
| AU2018353924A1 (en) | 2017-12-29 | 2019-07-18 | Clear Labs, Inc. | Automated priming and library loading device |
| US20230089107A1 (en) * | 2020-02-25 | 2023-03-23 | Institute For Environmental Health, Inc. | Incubator for enrichment during transport |
| EP4294521A4 (en) * | 2021-02-17 | 2025-01-08 | Cap Diagnostics, LLC, DBA Pathnostics | Methods and systems for determining suitability of compositions for inhibiting growth of polymicrobial samples |
| US20260103740A1 (en) * | 2022-09-15 | 2026-04-16 | General Electric Company | Method and apparatus for using lyophilized reagent beads for antimicrobial susceptibility testing |
| CN116218893B (zh) * | 2023-03-24 | 2025-07-29 | 哈尔滨医科大学 | 一种通过体外扩增重组质粒在其编码基因上生成短片段串联重复序列的方法 |
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| EP0853658A1 (en) * | 1995-09-29 | 1998-07-22 | Idexx Laboratories, Inc. | Self-contained incubator for growth of microorganism |
| WO2015189390A1 (en) * | 2014-06-13 | 2015-12-17 | Q-Linea Ab | Method for detecting and characterising a microorganism |
| US20160053219A1 (en) * | 2014-08-25 | 2016-02-25 | Opgen, Inc. | Systems, methods, and devices for temperature control |
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| US3159384A (en) | 1962-07-02 | 1964-12-01 | Bio Science Labor | Agitator for laboratory tubes and flasks |
| US3764780A (en) | 1971-06-16 | 1973-10-09 | C Ellis | Blood culture apparatus |
| US3975001A (en) | 1973-10-19 | 1976-08-17 | American Hospital Supply Corporation | Apparatus for mixing fluids held in tubes |
| GB1507026A (en) * | 1974-04-11 | 1978-04-12 | Nat Res Dev | Endoprosthetic devices |
| US4004883A (en) | 1975-07-11 | 1977-01-25 | G. D. Searle & Co. | Sensing, leveling and mixing apparatus |
| DE7922217U1 (de) | 1979-08-03 | 1979-11-22 | Binz Gmbh & Co, 7073 Lorch | Warmhaltebehaelter zum transport von infusionen o.dgl. |
| US4629686A (en) | 1982-02-19 | 1986-12-16 | Endotronics, Inc. | Apparatus for delivering a controlled dosage of a chemical substance |
| EP0131052B1 (en) | 1983-01-10 | 1993-12-08 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating organisms and viruses |
| EP0122581B1 (en) | 1983-04-15 | 1991-01-16 | TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION | Process for isolating bacteria in blood |
| US4666850A (en) | 1983-10-28 | 1987-05-19 | Becton, Dickinson And Company | Microbial pathogen detecting system and process |
| US4652517A (en) | 1984-06-11 | 1987-03-24 | Diagnostic Research Limited Partnership | Methods for the in vitro detection and identification of unknown pathogens or genetic entities |
| AU1363688A (en) | 1987-02-12 | 1988-09-14 | Sdi Diagnostics, Inc. | Test system, test device and method for detecting pathogenic organisms and antibiotic resistance genes in body fluids |
| US5217876A (en) | 1988-03-15 | 1993-06-08 | Akzo N.V. | Method for detecting microorganisms |
| CA2016699C (en) | 1989-05-15 | 2003-11-18 | Paul N. Marshall | Lytic agents and uses thereof |
| US6406844B1 (en) | 1989-06-07 | 2002-06-18 | Affymetrix, Inc. | Very large scale immobilized polymer synthesis |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US6346413B1 (en) | 1989-06-07 | 2002-02-12 | Affymetrix, Inc. | Polymer arrays |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5183994A (en) | 1990-10-26 | 1993-02-02 | Bowles Sr Dale D | Heated drug box |
| US5052812A (en) | 1990-11-19 | 1991-10-01 | New Brunswick Scientific Co., Inc. | Bath shaker |
| US5316146A (en) | 1991-03-06 | 1994-05-31 | Ulster Scientific, Inc. | Vial transporter |
| JP2565844B2 (ja) | 1992-02-07 | 1996-12-18 | アボツト・ラボラトリーズ | 細胞溶解処理条件下で異種細胞集団を正確に計数し，感受性に関する格付けを行う方法 |
| US5882918A (en) | 1995-08-08 | 1999-03-16 | Genespan Corporation | Cell culture incubator |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| EP0695941B1 (en) | 1994-06-08 | 2002-07-31 | Affymetrix, Inc. | Method and apparatus for packaging a chip |
| US20020055101A1 (en) | 1995-09-11 | 2002-05-09 | Michel G. Bergeron | Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| AU4586096A (en) | 1995-03-10 | 1996-09-19 | Becton Dickinson & Company | Sample processing method for whole blood |
| JPH08285839A (ja) | 1995-04-13 | 1996-11-01 | Daiichi Kigyo:Kk | 病理検体等運搬用容器 |
| US5577837A (en) | 1995-05-19 | 1996-11-26 | Forma Scientific, Inc. | Temperature controlled HEPA-filtered console shaker |
| US6096532A (en) | 1995-06-07 | 2000-08-01 | Aastrom Biosciences, Inc. | Processor apparatus for use in a system for maintaining and growing biological cells |
| SE506908C2 (sv) | 1995-09-08 | 1998-03-02 | Ulf Landegren Inst F Medicinsk | Medicinsk användning av padlockprober |
| US5994066A (en) | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| AU714486B2 (en) | 1995-11-21 | 2000-01-06 | Yale University | Unimolecular segment amplification and detection |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6093551A (en) | 1995-12-08 | 2000-07-25 | Martin Raithel | Process and device for in vitro diagnosis of allergic disorders |
| SE9601676D0 (sv) | 1996-04-30 | 1996-04-30 | Ulf Landegren | Improved probing of specific mucleic acids |
| US5770394A (en) | 1996-05-22 | 1998-06-23 | Becton Dickinson And Company | Method and apparatus for detecting bacteria using a blood culture froth |
| EP0822261B1 (en) | 1996-07-29 | 2002-07-03 | Academia Sinica | Methods for rapid antimicrobial susceptibility testing |
| US5817508A (en) | 1996-09-27 | 1998-10-06 | Becton Dickinson And Company | Blood culture apparatus having an auto-unloading and sorting device |
| CA2276462C (en) | 1996-12-31 | 2007-06-12 | High Throughput Genomics, Inc. | Multiplexed molecular analysis system apparatus and method |
| US6255467B1 (en) | 1997-11-06 | 2001-07-03 | Pathobiotek Diagnostics Inc. | Human blood bacterium |
| US6028293A (en) | 1998-04-20 | 2000-02-22 | Tcp Reliable Inc. | Temperature-controlled container with heating means |
| US6059446A (en) | 1998-05-08 | 2000-05-09 | Dschida; William J. A. | Apparatus for mixing the contents of microcentrifuge tubes |
| WO2000036142A1 (en) | 1998-12-11 | 2000-06-22 | Visible Genetics Inc. | METHOD AND KIT FOR THE CHARACTERIZATION OF ANTIBIOTIC-RESISTANCE MUTATIONS IN $i(MYCOBACTERIUM TUBERCULOSIS) |
| WO2000067037A2 (en) | 1999-04-29 | 2000-11-09 | Dade Microscan Inc. | A combined rapid anti-microbial susceptibility assay and microorganism identification system |
| JP3811616B2 (ja) | 1999-05-25 | 2006-08-23 | ミリポア・コーポレイション | 哺乳動物細胞調製物中の微生物を、ａｔｐ生物発光を利用して迅速に検出して計数する方法 |
| CA2373198C (en) | 1999-05-28 | 2011-10-11 | Cepheid | Cartridge for conducting a chemical reaction |
| JP2001103981A (ja) | 1999-08-03 | 2001-04-17 | Nisshinbo Ind Inc | 結核菌診断キット |
| US6465448B1 (en) | 1999-08-13 | 2002-10-15 | Case Western Reserve University | Methoxyamine potentiation of temozolomide anti-cancer activity |
| JP2001169778A (ja) | 1999-12-17 | 2001-06-26 | Joji Oshima | 迅速細菌遺伝子検査法及びキット |
| CN2436459Y (zh) | 2000-08-14 | 2001-06-27 | 青岛市家用电器研究所 | 一种血液运输用储藏箱 |
| US20040121309A1 (en) | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues |
| US7223363B2 (en) | 2001-03-09 | 2007-05-29 | Biomicro Systems, Inc. | Method and system for microfluidic interfacing to arrays |
| US6803208B2 (en) | 2001-07-30 | 2004-10-12 | The United States Of America As Represented By The Secretary Of The Navy | Automated epifluorescence microscopy for detection of bacterial contamination in platelets |
| US7008788B2 (en) | 2001-07-30 | 2006-03-07 | Agilent Technologies, Inc. | Containers for supports comprising biopolymers |
| GB2378245A (en) | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| EP2311934B1 (en) | 2001-09-06 | 2013-06-05 | Rapid Micro Biosystems, Inc. | Rapid detection of replicating cells |
| US20030098271A1 (en) | 2001-11-26 | 2003-05-29 | Ralph Somack | Capsule and tray systems for combined sample collection, archiving, purification, and PCR |
| DE10201463B4 (de) | 2002-01-16 | 2005-07-21 | Clondiag Chip Technologies Gmbh | Reaktionsgefäß zur Durchführung von Array-Verfahren |
| RU2228735C2 (ru) | 2002-04-10 | 2004-05-20 | Абанин Вячеслав Владимирович | Устройство для хранения концентрата тромбоцитов |
| DE10222133A1 (de) | 2002-05-17 | 2003-12-04 | Gl Biotech Gmbh | Verfahren zur Nukleinsäure-Extraktion und Nukleinsäure-Reinigung |
| WO2003097831A1 (en) | 2002-05-17 | 2003-11-27 | Gl Bio Tech Gmbh | Method for nucleic acid extraction and nucleic acid purification |
| JP2006516193A (ja) | 2002-12-06 | 2006-06-29 | アイシス・ファーマシューティカルス・インコーポレーテッド | ヒトおよび動物における病原体の迅速な同定方法 |
| US20060094034A1 (en) | 2003-04-30 | 2006-05-04 | Roland Brousseau | Virulence and antibiotic resistance array and uses thereof |
| AU2004265570B2 (en) | 2003-05-13 | 2009-01-08 | Gen-Probe Incorporated | Method and kit for identifying antibiotic-resistant microorganisms |
| ES2661168T3 (es) | 2003-07-12 | 2018-03-27 | Accelerate Diagnostics, Inc. | Biodetección sensible y rápida |
| US20120077206A1 (en) | 2003-07-12 | 2012-03-29 | Accelr8 Technology Corporation | Rapid Microbial Detection and Antimicrobial Susceptibility Testing |
| US7341841B2 (en) | 2003-07-12 | 2008-03-11 | Accelr8 Technology Corporation | Rapid microbial detection and antimicrobial susceptibility testing |
| US7205111B2 (en) * | 2003-07-24 | 2007-04-17 | Marshfield Clinic | Rapid identification of bacteria from positive blood cultures |
| US20050042144A1 (en) | 2003-08-18 | 2005-02-24 | A. Robert Hubbard | Portable insulated container for biological specimens |
| US20060286301A1 (en) | 2003-09-12 | 2006-12-21 | National Institute Of Advanced Industrial Science | Substrates and method of manufacturing same |
| WO2005059165A2 (en) | 2003-12-09 | 2005-06-30 | Biomerieux, Inc. | Methods for detecting bacterial pathogens |
| EP1692512B1 (en) | 2003-12-09 | 2012-09-05 | bioMérieux, Inc. | Methods for detecting bacterial pathogens |
| EP1574583A1 (en) | 2004-03-10 | 2005-09-14 | Roche Diagnostics GmbH | Methods for isolation of bacteria from biological samples |
| CA2559943A1 (en) | 2004-03-15 | 2005-10-06 | Purdue Research Foundation | Cell concentration and pathogen recovery |
| US7580920B2 (en) | 2004-07-22 | 2009-08-25 | Microsoft Corporation | System and method for graceful degradation of a database query |
| US7662562B2 (en) | 2004-08-10 | 2010-02-16 | Becton, Dickinson And Company | Method for rapid identification of microorganisms |
| DE102005009479A1 (de) | 2005-03-02 | 2006-09-07 | Molzym Gmbh & Co. Kg | Verwendung von Nukleasen zum Abbau von Nukleinsäure in Gegenwart von chaotropen Agenzien und/oder Tensiden |
| US20060223098A1 (en) | 2005-03-31 | 2006-10-05 | Lane David J | Circularizable nucleic acid probes and amplification methods |
| WO2007033051A2 (en) | 2005-09-12 | 2007-03-22 | Genzyme Corporation | Thermally insulated transport container for cell-based products and related methods |
| JP2009508490A (ja) | 2005-09-15 | 2009-03-05 | マイクロファージ・インコーポレーテッド | バクテリオファージを用いた、微生物同定のための方法および装置 |
| EP1951905B1 (en) | 2005-11-10 | 2012-04-11 | Affymetrix, Inc. | Detection of nucleic acids through amplification of surrogate nucleic acids |
| US20090209031A1 (en) | 2006-01-26 | 2009-08-20 | Tyco Healthcare Group Lp | Medical device package |
| US7334516B2 (en) | 2006-03-08 | 2008-02-26 | Taiwan Supercritical Technology Co., Ltd. | Aging device for liquor or wine |
| EP1945821B1 (en) | 2006-06-06 | 2011-01-19 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| JP5254226B2 (ja) | 2006-07-14 | 2013-08-07 | エクセレレックス インク． | 環境保護用収容システム |
| JP4872511B2 (ja) | 2006-08-02 | 2012-02-08 | 株式会社日立製作所 | 温度調整部材 |
| ATE517192T1 (de) | 2006-08-10 | 2011-08-15 | Merck Patent Gmbh | Verfahren zur isolierung von zellen |
| US8652782B2 (en) | 2006-09-12 | 2014-02-18 | Longhorn Vaccines & Diagnostics, Llc | Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids |
| US20080145919A1 (en) * | 2006-12-18 | 2008-06-19 | Franklin Thomas D | Portable organ and tissue preservation apparatus, kit and methods |
| CA2684311A1 (en) | 2007-04-18 | 2008-10-30 | Cornell University | Microorganism detection method and apparatus |
| CN101130808B (zh) | 2007-07-27 | 2011-08-17 | 南京市胸科医院 | 分枝杆菌快速培养药敏及鉴定微量检测法及装置 |
| JP2010535016A (ja) | 2007-08-02 | 2010-11-18 | ユニヴェルシテ・ラヴァル | 体液から微生物細胞および微生物核酸の濃縮および富化 |
| DE102007041864B4 (de) * | 2007-09-04 | 2012-05-03 | Sirs-Lab Gmbh | Verfahren zum Nachweis von Bakterien und Pilzen |
| EP2056114A1 (en) | 2007-10-29 | 2009-05-06 | Koninklijke Philips Electronics N.V. | Automatic detection of infectious diseases |
| EP2087934A1 (de) | 2008-02-07 | 2009-08-12 | Qiagen GmbH | Verfahren und Vorrichtung zur automatisierten Prozessierung einer Probe |
| DE102008029356A1 (de) | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
| US8512975B2 (en) | 2008-07-24 | 2013-08-20 | Biomerieux, Inc. | Method for detection and characterization of a microorganism in a sample using time dependent spectroscopic measurements |
| US20110269130A1 (en) | 2008-10-24 | 2011-11-03 | Song Shi | Antibiotice Susceptibility Profiling Methods |
| WO2010062356A1 (en) | 2008-10-31 | 2010-06-03 | Biomerieux, Inc. | Methods for separation, characterization and/or identification of microorganisms using spectroscopy |
| KR20110091719A (ko) | 2008-10-31 | 2011-08-12 | 바이오메리욱스, 인코포레이티드. | 식별제를 사용한 미생물의 분리 및 특성규명 방법 |
| WO2010062350A1 (en) * | 2008-10-31 | 2010-06-03 | Biomerieux, Inc. | Methods for detection, characterization and/or identification of microorganisms in a sealed container |
| DK200801722A (en) | 2008-12-05 | 2010-06-06 | Unisensor As | Optical sectioning of a sample and detection of particles in a sample |
| MX338624B (es) | 2009-05-15 | 2016-04-26 | Bio Merieux Inc | Aparato automatizado para la deteccion microbiana. |
| JP2012526996A (ja) | 2009-05-15 | 2012-11-01 | ビオメリュー・インコーポレイテッド | 試料内の微生物因子の迅速な同定および／または特徴付けのためのシステムおよび方法 |
| EP2464753A4 (en) | 2009-08-12 | 2013-12-11 | Harvard College | METHODS AND COMPOSITIONS FOR BIODETECTION |
| EP2325312A1 (de) | 2009-11-19 | 2011-05-25 | Qiagen GmbH | Verfahren zur selektiven Anreicherung und Isolierung mikrobieller und optional zusätzlich viraler Nukleinsäuren |
| EP2333105A1 (en) | 2009-12-08 | 2011-06-15 | Koninklijke Philips Electronics N.V. | Selective lysis of cells |
| JP6138491B2 (ja) | 2010-02-24 | 2017-05-31 | ザ ブロード インスティテュート， インコーポレイテッド | 感染性疾患の病原体およびそれらの薬剤感受性を診断する方法 |
| DE102010012790B4 (de) | 2010-03-25 | 2015-08-06 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Inkubatoreinrichtung und Verfahren zu deren Betrieb |
| CN101845390B (zh) | 2010-04-30 | 2013-09-11 | 武汉迪艾斯科技有限公司 | 自动化细菌培养仪 |
| EP2388312A1 (en) | 2010-05-17 | 2011-11-23 | Curetis AG | Universally applicable lysis buffer and processing methods for the lysis of bodily samples |
| JP2013531983A (ja) | 2010-06-11 | 2013-08-15 | パソジェニカ，インコーポレイテッド | 多重生物検出のための核酸ならびにその使用および製造方法 |
| AU2011279815B2 (en) | 2010-07-20 | 2015-06-18 | Biomerieux, Inc. | Detector arrangement for blood culture bottles with colorimetric sensors |
| CN103188994B (zh) * | 2010-11-04 | 2015-06-17 | 日立化成株式会社 | 用于体外刺激全血的便携设备 |
| CA2839794A1 (en) | 2011-05-20 | 2012-11-29 | Advandx, Inc. | Selective ultrasonic lysis of blood and other biological fluids and tissues |
| CA2831969A1 (en) | 2011-06-06 | 2012-12-30 | Biocartis S.A. | Selective lysis of cells by ionic surfactants |
| US10774300B2 (en) | 2011-07-22 | 2020-09-15 | Biomerieux, Inc. | Methods and kits for isolating microorganisms from culture |
| US8603769B2 (en) | 2011-10-07 | 2013-12-10 | Becton, Dickinson And Company | Method for direct and rapid identification of microorganisms and antimicrobial susceptibility testing from positive blood cultures |
| ES2665252T3 (es) | 2011-12-30 | 2018-04-25 | Abbott Molecular Inc. | Purificación de ácido nucleico de microorganismos a partir de muestras del hospedador |
| US10475527B2 (en) | 2012-03-22 | 2019-11-12 | Biomerieux, Inc. | Method and system for detection of microbial growth in a specimen container |
| WO2013163210A1 (en) | 2012-04-23 | 2013-10-31 | Philip Alexander Rolfe | Method and system for detection of an organism |
| JP6018808B2 (ja) | 2012-06-12 | 2016-11-02 | 株式会社日立ハイテクノロジーズ | 微生物検査方法および検査システム |
| EP2684947A1 (en) | 2012-07-09 | 2014-01-15 | Biocartis SA | A method for filtering a biological sample |
| WO2014040088A1 (en) | 2012-09-10 | 2014-03-13 | Accelerate Diagnostics, Inc. | Same-day blood culture with digital microscopy |
| WO2014076209A1 (en) | 2012-11-14 | 2014-05-22 | Olink Ab | Localised rca-based amplification method |
| US20140287408A1 (en) | 2013-03-13 | 2014-09-25 | Abbott Molecular Inc. | Target sequence enrichment |
| US9677109B2 (en) | 2013-03-15 | 2017-06-13 | Accelerate Diagnostics, Inc. | Rapid determination of microbial growth and antimicrobial susceptibility |
| GB201321123D0 (en) | 2013-11-29 | 2014-01-15 | Linea Ab Q | Amplification of circular molecules |
| GB2520765A (en) | 2013-12-02 | 2015-06-03 | Vanadis Diagnostics Ab | Multiplex detection of nucleic acids |
| EP2942394A1 (en) | 2014-05-09 | 2015-11-11 | Molzym GmbH & Co. KG | New method for isolating microbial DNA |
| CN204255722U (zh) | 2014-12-15 | 2015-04-08 | 王东生 | 血液孵育器 |
| AU2016212166B2 (en) | 2015-01-26 | 2019-10-17 | Ventana Medical Systems, Inc. | Transporter systems, assemblies and associated methods for transporting tissue samples |
| WO2016168803A1 (en) * | 2015-04-17 | 2016-10-20 | Vax-Immune, LLC | Portable disposable re-usable culture device for rapid diagnosis of infectious agents |
| GB201507026D0 (en) * | 2015-04-24 | 2015-06-10 | Linea Ab Q | Medical sample transportation container |
| GB201511129D0 (en) * | 2015-06-24 | 2015-08-05 | Linea Ab Q | Method of determining antimicrobial susceptibility of a microorganism |
| US10407659B2 (en) * | 2015-11-09 | 2019-09-10 | Sahar M. H Jaffal | Mini-incubator carrier box “Mini-incubator” |
-
2016
- 2016-10-13 GB GB1617353.6A patent/GB2554767A/en not_active Withdrawn
-
2017
- 2017-04-20 FR FR1753439A patent/FR3050461A1/fr not_active Ceased
- 2017-04-21 WO PCT/EP2017/059542 patent/WO2017182645A1/en not_active Ceased
- 2017-04-21 US US16/094,699 patent/US11845978B2/en active Active
- 2017-04-21 EP EP17719557.5A patent/EP3294057B1/en active Active
-
2023
- 2023-10-30 US US18/385,099 patent/US20240368670A1/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0853658A1 (en) * | 1995-09-29 | 1998-07-22 | Idexx Laboratories, Inc. | Self-contained incubator for growth of microorganism |
| WO2015189390A1 (en) * | 2014-06-13 | 2015-12-17 | Q-Linea Ab | Method for detecting and characterising a microorganism |
| US20160053219A1 (en) * | 2014-08-25 | 2016-02-25 | Opgen, Inc. | Systems, methods, and devices for temperature control |
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| US20240368670A1 (en) | 2024-11-07 |
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| US11845978B2 (en) | 2023-12-19 |
| EP3294057A1 (en) | 2018-03-21 |
| WO2017182645A1 (en) | 2017-10-26 |
| GB201617353D0 (en) | 2016-11-30 |
| US20190119724A1 (en) | 2019-04-25 |
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