GB1566171A

GB1566171A - Spiro hydantoin derivatives as therapeutic agents

Info

Publication number: GB1566171A
Application number: GB43122/77A
Authority: GB
Original assignee: Pfizer Inc
Current assignee: Pfizer Inc
Priority date: 1976-10-18
Filing date: 1977-10-17
Publication date: 1980-04-30
Also published as: CA1088945A; YU40821B; FI62307B; AU504978B2; FR2367754B1; PT67164B; FR2367754A1; IE45805B1; FI62307C; YU244277A; DE2759543C2; AT356136B; AU2943177A; DE2746244C2; PH15639A; JPS607997B2; DE2759927C2; NZ185358A; JPS5495570A; AR220901A1

Abstract

The compounds of formulae I to V - the meaning of the symbols is explained in Claim 1 - are prepared by a reaction of the starting carbonyl compound with simultaneously an alkali metal cyanide and ammonium carbonate. The yields are very good. If desired, the salts of the compounds obtained with pharmaceutically acceptable cations are formed. The compounds obtained can be used as medicaments for combating certain chronic complications resulting from alimentary diabetes. <IMAGE>

Description

(54) SPIROHYDANTOIN DERIVATIVES AS THERAPEUTIC AGENTS (71) We, PFIZER, INC., a Corporation organized under the laws of the State of Delaware, United States of America, of 235 East 42nd Street, New York, State of New York, United States of America, do hereby declare the invention, for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement:- This invention relates to new and useful hydantoin derivatives in the field of medicinal chemistry. More particularly, it is concerned with a novel series of spirohydantoin compounds, which are of especial value in view of their ability to control certain chronic complications arising from diabetes mellitus (e.g., diabetic cataracts and neuropathy). The invention also includes a new method of therapy for non-human beings within its scope.

In the past, various attempts have been made by numerous investigators in the field of organic medicinal chemistry to obtain new and better oral antidiabetic agents. For the most part, these efforts have involved the synthesis and testing of various heretofore new and unavailable organic compounds, particularly in the area of the sulfonylureas, in an endeavor to determine their ability to lower blood sugar (i.e., glucose) levels to a substantially high degree when given by the oral route of administration. However, in the search for newer and still more effective antidiabetic agents, little is known about the effect of other organic compounds in preventing or arresting certain chronic complications of diabetes, such as diabetic cataracts, neuropathy and retinopathy. Nevertheless, K. Sestanj et al. in U.S.

Patent No. 3,821,383 do disclose that certain aldose reductase inhibitors like 1,3 dioxo - 1H - benz[d,e]isoquinoline - 2(3H) - acetic acid and some closely-related derivatives thereof are useful for these purposes, even though these particular compounds are not known to be hypoglycemic in nature. These particular aldose reductase inhibitors all function by inhibiting the activity of the enzyme aldose reductase, which is primarily responsible for regulating the reduction of aldoses (like glucose and galactose) to the corresponding polyols) such as sorbitol and galactitol) in the human body. In this way, unwanted accumulations of galactitol in the lens of galactosemic subjects and of sorbitol in the lens, peripheral nervous cord and kidney of various diabetic subjects are thereby prevented or otherwise reduced as the case may be. As a result, these compounds are definitely of value as aldose reductase inhibitors for controlling certain chronic diabetic complications, including those of an ocular nature, since it is already known in the art that the presence of polyols in the lens of the eye invariably leads to cataract formation together with a concomitant loss of lens clarity.

In accordance with the present invention, it has now been rather surprisingly found that various spiro-hydantoin compounds are extremely useful when employed in therapy as aldose reductase inhibitors for the control of certain chronic diabetic complications in a host subject to whom they are administered.

More particularly, the novel method of treatment of the present invention involves treating a non-human diabetic host to prevent or alleviate diabetes-associated chronic ocular complications by administering to said host an effective amount of a compound of the formulae:

and the base salts thereof with pharmacologically acceptable cations, wherein W is (CH2)n; X is hydrogen and X' is hydrogen hydroxy, fluorine, chlorine, lower alkyl or lower alkoxy (the word lower is defined as having from one to four carbon atoms); or X and X1, when taken separately, are each chlorine, lower alkyl or lower alkoxy and when taken together are -OCH2(CH2)O-; Y is oxygen or sulfur; Z is W, Y or Q wherein Q is

and n is zero or one. These compounds are all potent aldose reductase inhibitors and therefore possess the ability to markedly reduce or even inhibit sorbitol accumulation in the lens and peripheral nerves of various diabetic subjects.

The novel compounds of this invention are those compounds of the formula III and also those compounds of the formula I comprising the compounds of the formula IV, V, VI and VII described below.

Accordingly, the novel compounds of formula I comprise spiro-hydantion compounds of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein X is hydrogen and X2 is fluorine, hydroxy or 6'-(lower alkoxy); or X and X2, when taken separately, are each lower alkoxy, and when taken together are CII2(CH2)nO, and n zero or one. The novel compounds of formula I also comprise spiro-hydantions of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein X3 is hydrogen and X4 iS fluorine, chlorine or bromine; or X3 and X4, when taken separately, are each chlorine and when taken together are -OCH2(CH2)O-; and n is zero or one.

The novel compounds of formula I additionally comprise spiro-hydantoins of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein X5 is hydrogen and Xe is fluorine, hydroxy or lower alkoxy; or X5 and X6, when taken separately, are each chlorine or lower alkoxy, and when taken together are -OCH2(CH2)nO-; Y is oxygen or sulfur; and n is zero or one.

Lastly, the novel compounds of formulaI also comprise spiro-hydantion. compounds of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein X7 is hydrogen and Xe is hydrogen fluorine, chlorine, bromine or lower alkoxy; or X7 and X8, when taken separately, are each chlorine or lower alkoxy, and when taken together are -OCH2(CH2)O-; Q is

and n is zero or one.

Of special interest in the treatment of non-human diabetic hosts are such typical and preferred member compounds as spiro - [imidazolidine - 4,1' indan] - 2,5 - dione, 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' dione, 6 - chloro - spiro - [chroman - 4,4' - imidazolidine]- 2',5' - dione. 6,7 dichloro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione, 6,8 - dichloro spiro - [chroman - 4,4' - imidazoline] - 2',5' - dione - 6' - fluoro - spiro [imidazoline - 4,4' - thiochroman] - 2',5' - dione and 6',7' - dichloro - spiro [imidazolidine - 4,4' - thiochroman] - 2,5 - dione, respectively. These particular compounds are all highly potent as regards their aldose reductase inhibitory activity in addition to being extremely effective in lowering sorbitol levels in the sciatic nerve and lens of diabetic subjects and galactitol levels in the lens of galactosemic subjects to a remarkably high degree. The preferred 6-fluoro and 6,8dichloro derivatives are, as previously indicated new compounds.

In accordance with the process employed for preparing the novel compounds of this invention, an appropriate carbonyl ring compound, such as the corresponding l-indanone, I-tetralone, 4-chromanone, thiochroman-4-one, thioindane - 3 - - one - 1,1 - dioxide, 4-oxoisothiochroman - 2,2 - dioxide thiochroman - 4 - one - I - oxide and thiochroman - 4 - one - 1,1 - dioxide of the respective formulae:

wherein W, X, X2, X3, X4, X5, X6, X7, X8, Y and Q are all as previously defined, is condensed with an alkali metal cyanide (e.g., sodium cyanide or potassium cyanide) and ammonium carbonate to form the desired spiro-hydantoin final product of the structural formulae previously indicated. This particular reaction is normally carried out in the presence of a reaction-inert polar organic solvent medium in which both the reactants and reagents are mutually miscible. Preferred organic solvents for use in this connection include cyclic ethers such as dioxane and tetrahydrofuran, lower alkylene glycols like ethylene glycol and trimethylene glycol, water-miscible lower alkanols such as methanol, ethanol and isopropanol, as well as N,N-di(lower alkyl) lower alkano-amides such as N,Ndimethylformamide, N,N-diethylformamide and N,N-dimethylacetamide. In generai, the reaction is conducted at a temperature from 200 C. up to 1 200C for a period of two hours to four days.

Although the amount of reactant and reagents employed in the reaction can vary to some extent, it is preferable to employ at least a slight molar excess of the alkali metal cyanide reagent with respect to the carbonyl ring compound starting material in order to effect maximum yield. Upon completion of the reaction, the desired product is easily isolated in a conventional manner, e.g., by first diluting the reaction mixture with water (boiling if necessary) and then cooling the resultant aqueous solution to room temperature, followed by acidification to afford the particular spiro-hydantoin compound in the form of a readily-recoverable precipitate. Needless to say, compounds of the invention in which Q of formula VII is

can be prepared from the corresponding compounds of formula VI wherein Y is sulfur by merely oxidizing the latter group of compounds in accordance with standard technique well known to those skilled in the art. For instance, the use of sodium periodate in this connection leads to the formation of the oxosulfur compounds, while peroxy acids such as peracetic acid, perbenzoic acid and mchloroperoxybenzoic acid, are preferably employed to afford the corresponding dioxosulfur compounds. On the other hand, certain compounds of the invention having a ring substituent which is hydroxy (X2 or X6) are usually preferably obtained by first preparing the corresponding alkoxy compounds where X2 or X6 is lower alkoxy (as previously defined) and then simply converting the latter to the desired hydroxy compound by cleavage of the ether moiety in a conventional manner.

The starting materials required for preparing the spiro-hydantoin compounds of this invention are, for the most part, known compounds and are either readily available commercially, like l-indanone and 6-chloro-4-chromanone, or else they can easily be synthesized by those skilled in the art starting from common chemical reagents and using conventional methods of organic synthesis. For instance 6fluoro-4-chromanone is obtained by condensing P-(p-fluorophenoxy)propionic acid in the presence of polyphosphoric acid, while 6,7-dichlorothiochroman-4-one is obtained by condensing p-(3,4-dichlorophenylthio)-propionic acid in the presence of concentrated sulfuric acid. In both cases. the starting organic acid is ultimately derived from a commercially available compound.

The chemical bases which are used as reagents in this invention to prepare the aforementioned pharmaceutically acceptable base salts are those which form nontoxic salts with the various herein described acidic spiro-hydantoin compounds, such as 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione, for example. These particular non-toxic base salts are of such a nature that their cations are said to be essentially non-toxic in character over the wide range of dosage administered. Examples of such cations include those of sodium, potassium, calcium and magnesium. These salts can easily be prepared by simply treating the aforementioned spiro-hydantoin compounds with an aqueous solution of the desired pharmacologically acceptable cation, and then evaporating the resulting solution to dryness while preferably being placed under reduced pressure.

Alternatively, they may also be prepared by mixing lower alkanolic solutions of the said acidic compounds and the desired alkali metal alkoxide together, and then evaporating the resulting solution to dryness in the same manner as before. In either case, stoichiometric quantities of reagents must be employed in order to ensure completeness of reaction and maximum production of yields with respect to the desired final product.

As previously indicated, the spiro-hydantoin compounds are all readily adapted to therapeutic use as aldose reductase inhibitors for the control of chronic diabetic complications, in view of their ability to reduce lens sorbitol levels in diabetic subjects to a statistically significant degree. For instance, 6 - fluoro spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione, a typical and preferred agent of the present invention, has been found to consistently control (i.e., inhibit) the formation of sorbitol levels in diabetic rats to a significantly high degree when given by the oral route of administration at dose levels ranging from 0.75 mg./kg. to 20 mg./kg., respectively, without showing any substantial signs of toxic side effects.

The other compounds of this invention also cause similar results. Furthermore, all the herein described compounds of this invention can be administered by either the oral or parenteral routes of administration, for the present purposes at hand, without causing any significant untoward pharmacological side reactions to occur in the subject to whom they are so administered. In general, these compounds are ordinarily administered in dosages ranging from 0.1 mg. to 10 mg. per kg. of body weight per day, although variations will necessarily occur depending upon the weight and condition of the subject being treated and the particular route of administration chosen.

In connection with the use of the spiro-hydantoin compounds for the treatment of diabetic subjects, it is to be noted that these compounds may be administered either alone or in combination with pharmaceutically acceptable carriers by either of the routes previously indicated, and that such administration can be carried out in both signals and multiple dosages. More particularly, the compounds of this invention can be administered in a wide variety of different dosage forms, i.e., they may be combined with various pharmaceuticallyacceptable inert carriers in the form of tablets, capsules, lozenges, troches, hard candies, powders, sprays, aqueous suspensions, injectable solutions, elixirs and syrups. Such carriers include solid diluents or fillers, sterile aqueous media and various non-toxic organic solvents. Moreover, such oral pharmaceutical formulations can be suitably sweetened and/or flavored by means of various agents of the type commonly employed for just such purposes. In general, the therapeutically useful compounds of this invention are present in such dosage forms at concentration levels ranging from 0.50/, to 90V by weight of the total composition, i.e., in amounts which are sufficient to provide the desired unit dosage.

For purposes of oral administration, tablets containing various excipients such as sodium citrate, calcium carbonate and calcium phosphate may be employed along with various disintegrants such as starch and preferably potato or tapioca starch, alginic acid and certain complex silicates, together with binding agents such as polyvinylpyrrolidone, sucrose, gelatin and acacia. Additionally, lubricating agents such as magnesium stearate, sodium lauryl sulfate and talc are often very useful for tabletting purposes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules; preferred materials in this connection would also include lactose or milk sugar as well as high molecular weight polyethylene glycols. When aqueous suspensions and/or elixirs are desired for oral administration, the essential active ingredient therein may be combined with various sweetening or flavoring agents, coloring matter or dyes, and if so desired, emulsifying and/or suspending agents as well, together with such diluents as water, ethanol, propylene glycol, glycerin and various combinations thereof.

For purposes of parenteral administration, solutions of these particular spirohydantoins in sesame or peanut oil or in aqueous propylene glycol may be employed, as well as sterile aqueous solutions of the corresponding water-soluble, alkali metal or alkaline-earth metal salts previously enumerated. Such aqueous solutions should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. These particular aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous and intraperitoneal injection purposes. In this connection, the sterile aqueous media employed are all readily obtainable by standard techniques well-known to those skilled in the art. Additionally, it is also possible to administer the aforesaid spirohydantoin compounds topically via an appropirate ophthalmic solution suitable for the present purposes at hand, which can then be given dropwise to the eye.

The activity of the compounds as agents for the control of chronic diabetic complications, is determined by their ability to successfully pass one or more of the following standard biological and/or pharmacological tests, viz., (1) measuring their ability to inhibit the enzyme activity of isolated aldose reductase; (2) measuring their ability to reduce or inhibit sorbitol accumulation in the sciatic nerve of acutely streptozotocinized (i.e., diabetic) rats; (3) measuring their ability to reverse already-elevated sorbitol levels in the sciatic nerve and lens of chronic streptozotocin-induced diabetic rats; (4) measuring their ability to prevent or inhibit galactitol formation in the lens of acutely galactosemic rats, and (5) measuring their ability to delay cataract formation and reduce the severity of lens opacities in chronic galactosemic rats.

Preparation A A mixture consisting of 3.5 g. (0.019 mole) of ,B-(p-fluorophenoxy)propionic acid [Finger et al., Journal of the American Chemical Society, Vol. 81, p. 94 (1959)] and 40 g. of polyphosphoric acid was heated on a steam bath for a period of ten minutes and then poured into 300 ml. of ice-water. The resulting aqueous mixture was next extracted with three separate portions of ethyl acetate, and the combined organic layers were subsequently washed with dilute aqueous sodium bicarbonate solution and then with water, followed by drying over anhydrous magnesium sulfate. After removal of the drying agent by means of filtration and the solvent by means of evaporation under reduced pressure, there was ultimately obtained a residue that was subsequently recrystallized from ethanol to afford 2.93 g. (93%) of pure 6-fluoro-4-chromanone, m.p. 114-116 C.

Anal. Calcd. for CgH7FOz 0.25 H2O:C, 63.34; H, 4.43.

Found: C, 63.24; H, 4.15.

Preparation B To a solution of 12.5 g (0.07 mole) of 3,4-dichlorobenzenethiol (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin), in 35 ml. of 2N aqueous sodium hydroxide and 5 ml, of ethanol, there was added an ice-cold solution consisting of 7.6 g. (0.07 mole) of -chloropropionic acid (also available from Aldrich) and 8.6 g. (0.07 mole) of sodium carbonate monohydrate dissolved in 50 ml. of water. The resulting reaction mixture was then heated on a steam bath for a period of two hours, cooled to room temperature (-250C.) and extracted with ethyl acetate to remove any impurities. The saved aqueous portion was then poured into 300 ml. of ice-cold 3N hydrochloric acid and the precipitated solids so obtained were subsequently collected by means of suction filtration. After washing the latter material with water, air-drying to constant weight and recrystallizing from ethyl acetate/n-hexane, there was obtained an 11.4 g. (65 u) yield of p-(3,4- dichlorophenylthio)-propionic acid, m.p. 70--720C.

Anal. Calcd. for C9H8CI2S: C, 43.04; H, 3.21.

Found: C, 43.13; H, 3.25.

A solution of the above product in concentrated sulfuric acid was prepared by adding 5.0 g. (0.02 mole) of A-(3,4-dichlorophenylthio)propionic acid to 50 ml. of ice-cold concentrated sulfuric acid, with constant agitation being maintained throughout the addition step. The resulting solution was then stirred at 0 C. for a period of 20 minutes and finally at room temperature for another 20 minutes. At this point, the entire reaction mixture was poured into 300 ml. of an ice-water mixture and the precipitated solids were collected by suction filtration, washed with water and air-dried to constant weight. Recrystallization from ethanol then gave 2.5 g. (54 / > ) of pure 6,7-dichlorothiochroman-4-one, m.p. 134--1360C.

Anal. Calcd. for C9H6Cl2OS: C, 46.37; H, 2.60.

Found: C, 46.34; H, 2.45.

Preparation C 3'4' - Dihydro- spiro - [imidazoline - 4,1'(2'H) - naphthalene] - 2,5 dione was prepared according to the procedure described in Chemical Abstracts, Vol. 35, p. 65767 (1941), starting from l-indanone and other readily available materials. The product obtained was identical in every respect with the prior art compound.

Example I A mixture consisting of 13.2 g. (0.1 mole) of l-indanone (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin), 9.75 g. (0.15 mole) of potassium cyanide and 28.8 g. (0.3 mole) of powdered ammonium carbonate in 200 ml. of 50% aqueous ethanol was heated in an oil bath at 750C. for a period of 24 hours. The reaction mixture was then diluted with 800 ml. of water, boiled for 15 minutes and after finally being cooled to room temperature, poured into 600 ml. of ice-cooled, concentrated hydrochloric acid. The resulting crystalline crop, which formed as a precipitate, was subsequently collected by means of suction filtration, washed with water and thereafter recrystallized from methanol-diethyl ether to afford 15.4 g. (76 /,) of pure spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione, m.p.

238-2400C. [Literature m.p. 239-2400C., according to Goodson, et al., Journal of Organic Chemistry, Vol. 25, p. 1920 (1960)].

Anal. Calcd. for C11H10N2O2: C, 65.33; H, 4.98; N, 13.86.

Found: C, 65.28; H, 5.01; N, 13.90.

Example II A mixture consisting of 2.5 g. (0.15 mole) of 6-methoxy-l-indanone (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin), 1.5 g. (0.23 mole) of potassium cyanide and 6.7 g. (0.07 mole) of ammonium carbonate in 20 ml. of ethanol was placed in a stainless-steel bomb and heated at 1 100C. for a period of 20 hours. After cooling to room temperature (-250C.), the contents of the bomb were diluted with 100 ml. of water and then acidified to pH 2.0 with 6N hydrochloric acid. The precipitated product so obtained was subsequently collected by means of suction filtration and thereafter recrystallized from ethanol to give 0.49 g. (14%) of pure 6' - methoxy - spiro - [imidazolidine - 4,1' - indan] 2,5 - dione, m.p. 192-1940C.

Anal. Calcd. for C12H,2N203: C, 62.06: H, 5.21; N, 12.06.

Found: C, 61.94; H, 5.26; N, 12.01.

Example III The procedure described in Example II was repeated except that 6-fluoro-1- indanone [Chemical Abstracts, Vol. 55, p. 25873a (1961)] was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 6' fluoro - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione, m.p. 255-2570C. The yield of pure product was 4.6% of the theoretical value.

Anal. Calcd. for CHa9FN202 C, 60.00; H, 4.12; N, 12.72.

Found: C, 59.86; H, 4.33; N, 12.49.

Example IV The procedure described in Example II was repeated except that 5,6dimethoxy-l-indanone [Koo, Journal of the American Chemical Society, Vol. 75, p.

1891(1953)] was the starting material employed in place of 6-methoxy-1-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 5',6' - dimethoxy - spiro [imidazolidine - 4,1' - indan] - 2,5 - dione, m.p. 246-2480C. The yield of pure product was 48% of the theoretical value.

Anal. Calcd. for C,3H,4N204: C, 59.53; H, 5.38; N, 10.68.

Found: C, 59.26; H, 5.49; N, 10.54.

Example V The procedure described in Example II was repeated except that 5,6 methylenedioxy-l-indanone [Perkin and Robinson, Journal of the Chemical Society, Vol. 91, p. 1084(1907)] was the starting material employed in place of 6-methoxy-l indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 5',6' - methylenedioxy - spiro [imidazolidine - 4,1' - indan] - 2,5 - dione, m.p. 248-2500C. The yield of pure product was 29% of the theoretical value.

Anal. Calcd. for C,2H,ON2Q4: C, 58.53; H, 4,09; N, 11.38.

Found: C, 58.44; H, 4.14; N, 11.25.

Example VI The procedure described in Example II was repeated except that 5-methoxy-l indanone (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin) was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 5' - methoxy - spiro - [imidazolidine 4,1' - indan] - 2,5 - dione, m.p. 167-1690C. The yield of pure product was 19 of the theoretical value.

Anal. Calcd. for C,2H12N203: C, 62.06; H, 5.21; N, 12.06.

Found: C,61.77; H,5.23; N, 12.14.

Example VII The procedure described in Example II was repeated except that thiochroman-4-one (available from Pfaltz & Bauer, Inc. of Stamford, Connecticut) was the starting material employed in place of 6-methoxy-1-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione, m.p. 225-227 C. (literature m.p. 222-227 C., according to West German Auslegeschrift No. 1,135,915). The yield of pure product was 44q/, of the theoretical value.

Example VIII The procedure described in Example II was repeated except that 6methoxythiochroman-4-one [Chemical Abstracts. Vol. 53, p. 7161c (1959)] was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 6' - methoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione, m.p. 170-172 C. The yield of pure product was 41% of the theoretical value.

Anal. Calcd. for C,2H,2N203S: C, 54.53; H, 4.58; N, 10.61.

Found: C, 54.64; H, 4.67: N, 10.66.

Example IX The procedure described in Example II was repeated except that 6chlorothiochroman-4-one [Chemical Abstracts, Vol. 55, p. 12397c (1961)] was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 6' - chloro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione, m.p. 244-246 C. The yield of pure product was 53% of the theoretical value.

AnaL Calcd. forC11H9ClN2O2S: C, 49.16; H, 3.38; N, 10.43.

Found: C, 49.23; H, 3.40; N, 10.39.

Example X The procedure described in Example II was repeated except that 6bromothiochroman-4-one [Arndt, Chemische Berichte, Vol. 58, p. 1612 (1925)J was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 6 - bromo - spiro - [imidazolidine - 4,4' - thiochroman] 2,5 - dione, m.p. 234-236 The yield of pure product was 56% of the theoretical value.

AnaL Calcd. for C1,HgBrN202S: C, 42.18; H, 2.90: N, 8.95.

Found: C, 41.98; H,2.92; N, 8.95.

Example XI The procedure described in Example II was repeated except that 6,7dichlorothiochroman-4-one (prepared as described in Preparation A) was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the cor

Example XIII The procedure described in Example II was repeated except that 8chlorothiochroman-4-one [Chemical Abstracts. Vol. 53, p. 716 it (1959)] was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 8' - chloro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione, m.p. 265267CC. The yield of pure product was 66?n of the theoretical value.

AnaL Calcd. for C11H9ClN2O2S: C, 49.16; H, 3.38; N, 10.43.

Found: C, 49.32; H, 3.50; N, 10.38.

Example XIV The procedure described in Example II was repeated except that 7chlorothiochroman-4-one [Chemical Abstracts, Vol. 52, p. 11044b (1958)] was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 7' - chloro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione, m.p. 235-2370C. The yield of pure product was 67 /" of the theoretical value.

Anal. Calcd. for C11H9ClN2O3S: C, 49.16; H, 3.38; N, 10.43.

Found: C, 49.32; H, 3.36; N, 10.03.

Example XV The procedure described in Example II was repeated except that 7,8dihydroquinolin-5(6H)-one (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin) was the starting material employed in place of 6-methoxy I-indanone, using the same molar proportions as before. In this particular case the corresponding final product obtained was 7'8' - dihydro - spiro - [imidazolidine 4,5'(6H) - quinoline] - 2,5 - dione, m.p. 275-277 C. The yield of pure product was 39% of the theoretical value.

Anal. Calcd. for C1,Ha,N302: C, 60.82; H, 5.10; N, 19.35.

Found: C, 60.41; H, 5.28; N, 19.29.

Example XVI The procedure described in Example II was repeated except that 7-methoxy-1tetralone (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin) was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 3',4' - dihydro - 7' - methoxy - spiro [imidazolidine - 4,1'(2'H)naphthalene] - 2,5 - dione, m.p. 227-2290C. The yield of pure product was 59% of the theoretical value.

Anal. Calcd. for Cl3H,4N203: C, 63.40; H, 5.73; N, 11.38.

Found: C, 63.19; H, 5.69; N, 11.30.

Example XVII The procedure described in Example II was repeated except that 6,7dimethoxytetralone [Howell and Taylor, Journal of the Chemical Society, p. 1248 (1958)] was the starting material employed in place of 6-methoxy-l-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 3'4' - dihydro - 6'7' - dimethoxy - spiro [imidazolidine - 4,1'(2H)naphthalene] - 2,5 - dione, m.p.238-240 C. The yield of pure product was 49 /a of the theoretical value.

Anal. Calcd. for C,4Hr6N204: C, 60.86; H, 5.84; N, 10.14.

Found: C, 60.94; H, 6.04; N, 10.48.

Example XVIII The procedure described in Example II was repeated except that 6-methoxy-ltetralone (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin) was the starting material employed in place of 6-methoxy-1-indanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 3',4' - dihydro - 6' - methoxy - .spiro [imidazoline - 4,1'(2'H)naphthalene] - 2,5 - dione, m.p. 219-221 C. (literature m.p. 219-220 C., according to U.S. Patent No. 3,532,744).

Example XIX A solution of 1.18 g. (0.005 mole) of 6' - methoxy - spiro - [imidazolidine 4,1' - indan] - 2,5 - dione (prepared as described in Example II) in 10 ml. of methylene chloride was cooled to -650C. and there was subsequently added thereto, in a dropwise manner, a solution consisting of 1.44 ml. (0.015 mole) of boron tribromide dissolved in 10 ml of methylene chloride, while stirring the entire reaction mixture under a nitrogen atmosphere, the resulting mixture was then allowed to attain room temperature (~25 C.) via removal of the cooling bath and thereafter kept at that point for a period of seven hours. Upon completing this step, 30 ml. of water were added to the mixture in a dropwise manner and the separated organic layer was subsequently collected and dried over anhydrous magnesium sulfate. After removal of the organic solvent (i.e., methylene chloride) by means of evaporation under reduced pressure, there was ultimately obtained a residual material that was subsequently recrystallized from ethanol to give 240 mg. (22 ") of pure 6' - hydroxy - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione, m.p. 253 255 C.

Anal. Calcd. for C11H10N2O3: C,60.54; H,4.62; N,12.84.

Found: C,60.29; H,4.66; N,12.93.

Example XX A mixture consisting of 5.0 g. (0.033 mole) of 4-chromanone (available from the Aldrich Chemical Company, Inc., of Milwaukee, Wisconsin), 2.8 g. (0.043 mole) of potassium cyanide and 8.26 g. (0.086 mole) of powdered ammonium carbonate at 40 ml, of ethanol was placed in a stainless-steel bomb and heated to 60 C. in an oil bath for a period of 24 hours. The reaction mixture was then diluted with 300 ml, of water, boiled for 15 minutes and after finally being cooled to room temperature, acidified with 6 N hydrochloric acid. The precipitated product so obtained was then collected by means of suction filtration and subsequently recrystallized from ethanol to give 2.5 g. (35%) of pure spiro - [chroman - 4,4' imidazolidine] - 2',5' - dione, m.p. 236-238 C. (literature m.p. 236-242 C., according to West German Auslegeschrift No. 1,135,915).

AnaL Calcd. for C1,H,1NO3: C, 64.38; H, 5.40; N, 6.83.

Found: C, 64.18; H, 5.38; N, 6.83.

Example XXI The procedure described in Example XX was repeated except that 6-methoxy4-chromanone (British Patent No. 1,024,645) was the starting material employed in place of 4-chromanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 6 - methoxy - spiro [chroman - 4,4' - imidazolidine] - 2',5' - dione, m.p. 170--1720C. the yield of pure product was 32% of the theoretical value.

Anal. Calcd. for C12H12N2O4: C, 58.06; H,4.87; N, 11.29.

Found: C, 58.04; H, 4.98; N, 11.17.

Example XXII The procedure described in Example XX was repeated except that 6-fluoro-4 chtomanone (prepared as described in Preparation B) was the starting material employed in place of 4-chromanone, using the same molar proportions as before.

In this particular case, the corresponding final product obtained was 6 - fluoro spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione, m.p. 239-2410C. The yield of pure product was 36% of the theoretical value.

Anal. Calcd. for C"HgFN203 C, 55.93; H, 3.84; N, 11.86.

Found: C, 55.54; H, 3.88; N, 12.12.

Example XXIII The procedure described in Example XX was repeated except that 6,7dichloro-4-chromanone (West German Offenlegungschrift No. 1,928,027) was the starting material employed in place of 4-chromanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 6,7 - dichloro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' dione, m.p. 263-2650C. the yield of pure product was 8% of the theoretical value.

Anal. Calcd. for C,1HSCI2N203: C, 46.02; H, 2.81; N, 9.76.

Found: C, 45.83; H, 2.94; N, 9.65.

Example XXIV The procedure described in Example XX was repeated except that 6,8dichloro-4-chromanone [Huckle et al., Journal of Medicinal Chemistry, Vol. 12, p.

277 (1969)] was the starting material employed in place of 4-chromanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 6,8 - dichloro - spiro - [chroman - 4,4' - imidazolidine] 2',5'- - dione, m.p. 234--2350C. The yield of pure product was 20% of the theoretical value.

AnaL Calcd. for C11H8C12N2O3: C, 46.02; H, 2.81; N, 9.76.

Found: C, 45.81; H, 2.74; N, 9.69.

Example XXV A mixture consisting of 4.57 g. (0.025 mole) of 6-chloro-4-chromanone (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin), 2.8 g. (0.043 mole) of potassium cyanide and 9.6 g. (0.1 mole) of powdered ammonium carbonate in 62.5 ml. of 50% aqueous ethanol was heated to 600 C. for a period of 48 hours. The reaction mixture was then cooled to room temperature (~25 C.), diluted with 300 ml. of water and thereafter acidified with 6N hydrochloric acid. The precipitated solids so obtained were subsequently collected by means of suction filtration and thereafter recrystallized from ethanol to yield 5.1 g. (81%) of pure 6 chloro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione, m.p. 268-2700C.

(literature m.p. 267-270 C., according to West German Auslegeschrift No.

1,135,915).

AnaL Calcd. for C11H9C1N2O3: C, 52.29; H, 3.59; N, 11.09.

Found: C, 52.15; H, 3.73; N, 10.99.

Example XXVI The procedure described in Example XXV was repeated except that 5methoxy-l-tetralone (available from the Aldrich Chemical Company, Inc., Milwaukee, Wisconsin) was the starting material employed in place of 6-chloro-4chromanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 3',4' - dihydro - 5' - methoxy spiro - [imidazolidine - 4,1'(2'H) - naphthalene] - 2,5 - dione, m.p. 243-243.50C.

(literature m.p. 242-242.50C., according to Sarges et al., Journal of Medicinal Chemistry, Vol. 16, p. 1003 (1973)].

Anal. Calcd. for C13H14N2O3: C, 63.40; H, 5.73; N, 11.38.

Found: C, 63.10; H, 5.70; N, 11.47.

Example XXVII The procedure described in Example XXV was repeated except that 8-chloro4-chromanone [Chemical Abstracts, Vol. 34, p. 47358 (1940)] was the starting material employed in place of 6-chloro-4-chromanone, using the same molar proportions as before. In this particular case, the corresponding final product obtained was 8 - chloro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione, m.p. 231-2330C. (literature m.p. 231-2350C., according to West German Auslegeschrift No. 1,135,915). The yield of pure product was 34% of the theoretical value.

Anal. Calcd. for C11H9ClN2O3: C, 52.29; H, 3.59; N, 11.09.

Found: C, 52.21; H, 3.74; N,11.12.

Example XXVIII The procedure described in Example XXV was repeated except that 6-bromo4-chromanone [Gilman et al., Journal of the American Chemical Society, Vol. 73, p.

4205 (1951)] was the starting material employed in place of 6-chloro-4chromanone, using the same molar proportions as before and the reaction temperature was 550C. instead of 60"C. In this particular case, the corresponding final product obtained was 6 - bromo - spiro - [chroman - 4,4' - imidazolidine] 2',5' - dione, m.p. 266--268"C. (literature m.p. 264--2690C., according to West German Auslegeschrift No. 1,135,915). The yield of pure product was 15n e of the theoretical value.

Example XXIX To a solution of 1.09 g. (0.005 mole) of spiro - [chroman - 4,4' imidazolidinel - 2',5' - dione (prepared as described in Example XX) and 10 mg. of ferric chloride in 6 ml. of dry dimethylformamide cooled to -400C., there was added in a dropwise manner and with constant agitation a solution consisting of 355 mg. of chlorine gas dissolved in 4 ml. of dry dimethylformamide. The resulting reaction mixture was then maintained at -400C. for a period of 30 minutes (with stirring) before being allowed to attain room temperature (--250C.). After being kept at the latter point for a period of 2.5 hours, it was poured into 250 ml. of icecold water to afford a crystalline precipitate that was subsequently collected by means of suction filtration and then air-dried to constant weight. Recrystallization of the latter material from glacial acetic acid (6 ml.) then gave 0.31 g. (25%) of pure 6 - chloro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione that was identical in every respect with the product of Example XXV.

Example XXX A mixture consisting of 252 mg. (0.001 mole) of 6' - fluoro - spiro [imidazolidine - 4,4' - thiochroman] - 2,5 - dione (prepared as described in Example XII) in 10 ml. of methylene chloride, together with 50 mg. of a 40% aqueous solution of tetrabutylammonium hydroxide and 224 mg. (0.01 mole) of sodium periodate in 5 ml. of water was stirred at room temperature (--250C.) for a period of one hour. The precipitated solids so obtained were subsequently collected by means of suction filtration and thereafter recrystallized from ethanol (3 ml.) to yield 60 mg. (22%) of pure 6' - fluoro - spiro - [imidazolidine - 4,4' thiochroman] - 2,5 - dione - 1' - oxide, m.p. 289-2910C.

Anal. Calcd. for C11H9FN2O3S: C, 49.25; H, 3.38; N, 10.44.

Found: C, 49.27; H, 3.35; N, 10.35.

Example XXXI To a suspension of 0.595 g. (0.00236 mole) of 6' - fluoro - spiro [imidazolidine - 4,4' - thiochroman] - 2,5 - dione (prepared as described in Example XII) in 50 ml. of chloroform contained in a 250 ml. three-necked round bottomed reaction flask, there was added in small portions over a one-hour period 1.00 g. (0.00579 mole) of m-chloroperoxybenzoic acid. The resulting slurry was then stirred at room temperature (--250C.) for a period of 36 hours and finally diluted with 500 ml. of ethyl acetate. The yellow organic layer so obtained was next washed with four 50 ml. portions of saturated aqueous sodium bicarbonate solution, dried over anhydrous magnesium sulfate, filtered and the solvent portion then removed in vacuo to afford 0.50 g. (74.5%) of crude 6' - fluoro - spiro - [imidazolidine 4,4' - thiochroman] - 2,5 - dione - 1', 1' - dioxide in the form of a white crystalline residue. Recrystallization from ethanol/ethyl acetate/n-hexane then gave the pure material (m.p. 179--1800C. with decomp.) as a first crop of fine white crystals (yield, 0.295 g.). Two additional recrystallizations from ethanol/ethyl acetate raised the melting point of the analytical sample to 184--186"C. (decomp.).

Anal. Calcd. for C11H9FN2O4S .0.5 CH3COOC2Hs: C, 47.55: H, 3.99; N, 8.53.

Found: C,47.54; H,3.93; N,8.56.

Example XXXI I The procedure described in Example XXXI was repeated except that 0.234 g.

(0.001 mole) of spiro - [imidazolidine - 4,4' - thiochroman] - 2.5 - dione (prepared as described in Example VII) and 0.426 g. (0.00247 mole) of m-chloro peroxybenzoic acid were reacted together to afford 0.20 g. (75 /n) af pure spiro [imidazolidine - 4,4' - thiochroman] - 2,5 - dione - I ', I ' - dioxide.

Recrystallization from methanol/ethanol/n-hexane then gave the analytical sample (m.p. 280-281 C.).

AnaL Calcd. for C11H10N2O4S: C, 49.61; H, 3.78; N, 10.52.

Found: C, 49.82; H, 3.85; N, 10.19.

Example XXXIII A mixture consisting of 1.0 g. (0.00549 mole) of thioindane-3-one-l,l-dioxide [Regitz, Chemische Berichte, Vol. 98, p. 36 (1965)], 0.613 g. (0.0094 mole) of potassium cyanide and 21.9 (0.021 mole) of ammonium carbonate in 14 ml. of 50 ^ aqueous ethanol was placed in a 50 ml. round-bottomed reaction flask and heated at 60"C. for a period of 48 hours while under a nitrogen atmosphere. The reaction mixture was then diluted with 70 ml. of water, a trace of solid was removed by means of filtration and the filtrate was subsequently acidified with 6 N hydrochloric acid. The precipitated product obtained in this manner was thereafter recovered by filtration, redissolved in 4 N aqueous potassium hydroxide and finally reacidified with 6 N hydrochloric acid. The acidified solution containing the product was saturated with sodium chloride and then extracted with six-150 ml. portions of fresh ethyl acetate, with the resulting organic layers subsequently being combined and dried over anhydrous magnesium sulfate. Upon removal of the drying agent by means of filtration and the organic solvent by means of evaporation under reduced pressure, there was obtained 0.50 g. (36 ,') of pure spiro - [imidazolidine - 4,3' thioindan] - 2,5 - dione - 1',1' - dioxide, m.p. 2870C. (decomp.) after two recrystallizations from ethanol/ethyl acetate/n-hexane.

Anal. Calcd.forC,OH8N204S: C, 47.61; H, 3.20; N, 11.11.

Found: C, 47.77; H, 3.28; N, 10.85.

Example XXXIV A mixture consisting of 2.75 g. (0.01562 mole) of 6,8-dimethyl-4-chromanone [Chemical Abstracts, Vol. 58, p. 13900c (1964)], 3.5 g. (0.0538 mole) of potassium cyanide and 10.5 g. (0.109 mole) of ammonium carbonate in 60 ml. of 50 /a aqueous ethanol was placed in a 125 ml. round-bottomed reaction flask and heated via an oil bath at 65"C. for a period of 48 hours while under a nitrogen atmosphere. The reaction mixture was then cooled to room temperature (-250C.) and filtered and the resulting filtrate subsequently extracted with 50 ml. of diethyl ether. The resulting aqueous layer was then saved and subsequently acidifed to pH 2.0 with 3 N hydrochloric acid (cooling was necessary). The cloudy mixture so obtained was next extracted with three-200 ml. portions of ethyl acetate and the combined organic layers were thereafter re-extracted with three-50 ml. portions of 4 N aqueous potassium hydroxide. The combined basic aqueous layers were reacidified again to pH 2.0 with 3 N hydrochloric acid in the same manner as before and then saturated with sodium chloride prior to extraction with three-200 ml. portions of fresh ethyl acetate. The combined organic layers were subsequently dried over an anhydrous magnesium sulfate and filtered. Upon removal of the solvent from the filtrate by means of evaporation under reduced pressure, there was ultimately obtained 2.50 g. (65 /n) of 6,8 - dimethyl - spiro - [chroman- 4,4' imidazolidine] - 2',5' - dione, m.p. 185--190"C. (decomp.). Two recrystallizations from aqueous ethanol then gave analytically pure material (m.p. 188-1890C.).

AnaL Calcd. for C,3H,4N203: C, 63.40; H, 5.73; N, 11.38.

Found: C, 63.05; H, 5.69; N, 11.33.

Example XXXV The following spiro-hydantoin compounds are prepared by employing the procedures described in the previous examples, starting from readily available materials in each instance: 6' - chloro - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 6' - bromo - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 5' - fluoro - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 5' - methyl - spiro - [imidazolidine - 4',1 - indan] - 2,5 - dione 6' - (n - butyl) - spiro - [imidazolidine - 4',1 - indan] - 2,5 - dione 5' - hydroxy - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 6' - ethoxy - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 5' - (n - butoxy) - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 5',6' - chloro - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 5',6' - dimethyl - spiro - [imidazolidine - 4',1 - indan] - 2,5 - dione 5',6' - di(n - propyl) - spiro - [imidazolidine - 4',1 - indan] - 2,5 - dione' - di(n - propoxy) - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 5',6' - di(n - propoxy) - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 5',6' - ethylenedioxy - spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione 8 - bromo - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 6 - (n - butyl) - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 7 - methyl - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 6 - hydroxy - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 6 - ethoxy - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 6 - (n - butoxy) - spiro - [chroman - 4,4' - imidazolidine - 2',5' - dione 7 - isopropoxy - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 6,8 - di(n - butyl) - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 6,7 - dimethoxy - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 6,8 - di(n - butoxy) - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 6,7 - ethylenedioxy - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione 8' - fluoro - spiro - [imidazolidine - 4,4' - thiochromani - 2,5 - dione 7' - bromo - spiro - [imidazolidine - 4,4' - thiochromanl - 2,5 - dione 6' - hydroxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 6' - methyl - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 7' - (n - butyl) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 7' - (n - butoxy) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 6' - isopropoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 6',8' - dichloro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 6',7' - dimethyl - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 6',8' - di(n - butyl) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione 6',7' - dimethoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 6',7' - diethoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 6',8' - di(n - butoxy) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione 6',7' - methylenedioxy - spiro - [imidazolidine - 4,4' - thiochromanj - 2,5 dione 6',7' - ethylenedioxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione - 1' - oxide 8' - chloro - spiro -[imidazolidine - 4,4' - thiochroman] - 2,5 - dione - I ' oxide 6' - bromo - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione - 1' oxide 6' - methyl - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione - 1' oxide 7' - (n - butyl) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1' - oxide 6' - methoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1' - oxide 7' - (n - butoxy) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1' - oxide 6',7' - dichloro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1' - oxide 6',7' - dimethyl - spiro - [imidazolidine -4,4' - thiochroman] - 2,5 - dione 1' - oxide 6',8' - di(n - butyl) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione - 1' - oxide 6',7' - dimethoxy - spiro - [imidazole - 4,4' - thiochroman] - 2,5 - dione 1' - oxide 6',7' - diethoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1' - oxide 6',8' - di(n - butoxy) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione - 1' - oxide 6',7' - methylenedioxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione - 1' - oxide 6',7' - ethylenedioxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione - I - oxide 8' - chloro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1',1' - dioxide 6' - methyl- spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1',1' - dioxide 7' - (n - butyl) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1',1' - dioxide 6' - methoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1',1' - dioxide 7' - (n - butoxy) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1',1' - dioxide 6',7' - dichloro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1',1' - dioxide 6',7' - dimethyl - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1',1' - dioxide 6',7' - dimethoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione - 1',1' - dioxide 6',7' - diethoxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione 1',1' - dioxide 6',8' - di(n - butoxy) - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione - 1',1' - dioxide 6',7' - methylenedioxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione - 1',1' - dioxide 6',7' - ethylenedioxy - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione - 1',1' - dioxide spiro - [imidazolidine - 4,4' - isothiochroman] - 2,5 - dione - l',1' - dioxide 3',4' - dihydro - 7' - fluoro - spiro - [imidazolidine - 4,1' - (2'H) naphthalene] - 2,5 - dione 3',4' - dihydro - 7' - chloro - spiro - [imidazolidine - 4,l'(2'H) - naphthalene] - 2,5 - dione 3',4' - dihydro - 6' - bromo - spiro - [imidazolidine - 4,1'(2'H) - naphthalene] - 2,5 - dione 3',4' - dihydro - 5' - isopropyl - spiro - [imidazolidine - 4,1' - (2'H) naphthalene] - 2,5 - dione 3',4' - dihydro - 6' - methyl- spiro - [imidazolidine - 4,1'(2'H)naphthalene] - 2,5 - dione 3',4' - dihydro - 7' - (n - butyl) - spiro - [imidazolidine - 4,1 '(2'H) naphthalene] - 2,5 - dione 3',4' - dihydro - 5' - hydroxy - spiro - [imidazolidine - 4,1 '(2'H) naphthalene] - 2,5 - dione 3',4' - dihydro - 5' - ethoxy - spiro - [imidazolidine - 4,1'(2'H) - naphthalene] - 2,5 - dione 3'4' - dihydro - 7' - (n - butoxy) - spiro - [imidazolidine - 4,1'(2'H) naphthalene] - 2,5 - dione 3',4' - dihydro - 6',7' - dichloro - spiro - [imidazolidine - 4,1'(2'H) - naphthalene] - 2,5 - dione 3',4' - dihydro - 6',7' - diethyl - spiro - [imidazolidine - 4,1'(2'H) naphthalene] - 2,5 - dione 3',4' - dihydro - 6',7' - dimethoxy - spiro - [imidazolidine - 4,1 '(2'H) naphthalene] - 2,5 - dione 3',4' - dihydro - 6,7 - (n - propoxy) - spiro - [imidazolidine - 4,1'(2'H) - naphthalenel - 2,5 - dione 3',4' - dihydro - 6',7' - methylenedioxy - spiro - [imidazolidine 4,1'(2'H) - naphthalene] - 2,5 - dione 3',4' - dihydro - 6',7' - ethylenedioxy - spiro - [imidazolidine - 4,1'(2'H) - naphthalene] - 2,5 - dione 6',7' . dihydro - spiro - [imidazolidine - 4,5'(5H) - pyridine] - 2,5 - dione Example XXXVI The sodium salt of 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] 2',5' - dione is prepared by dissolving said compound in water containing an equivalent amount in moles of sodium hydroxide and then freeze-drying the mixture. In this way, the desired alkali metal salt of the hydantoin is obtained in the form of an amorphous powder which is freely soluble in water.

In like manner, the potassium and lithium salts are also similarly prepared, as are the alkali metal salts of all the other spiro-hydantoin compounds of this invention which are reported in Examples I-XXI and XXIII-XXXV, respectively Example XXXVII The calcium salt of 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] 2',5' - dione is prepared by dissolving said compound in water containing an equivalent amount in moles of calcium hydroxide and then freeze-drying the mixture. The corresponding magnesium salt Percent Inhibition (%) Compound 10-4M 10-5M 10-6M 10-7M Prod. of Prep. C 72 34 13 7 Prod. of Ex. I 73 39 18 -11 Prod. of Ex. II 97 61 17 1 Prod. of Ex. III 74 12 22 -1 Prod. of Ex. IV 90 81 35 9 Prod. of Ex. V 92 67 25 3 Prod. of Ex. VI 82 60 13 -10 Prod. of Ex. VII 92 64 10 -5 Prod. of Ex. VIII 76 60 18 7 Prod. of Ex. IX 79 87 71 30 Prod. of Ex. X 32 - - Prod. of Ex. XI 67 84 76 69 Prod. of Ex. XII 81 77 66 38 Prod. of Ex. XIII 60 - - Prod. of Ex. XIV 70 - - Prod. of Ex. XV 83 54 9 -2 Prod. of Ex. XVI 54 - 23 Prod. of Ex. XVII 82 26 8 16 Prod. of Ex. XVIII 72 38 15 7 Prod. of Ex. XIX 93 31 11 -30 Prod. of Ex. XX 73 64 -9 -16 Prod. of Ex. XXI 100 92 35 7 Prod. of Ex. XXII 84 58 52 3 Prod. of Ex. XXIII 59 96 91 84 Prod. of Ex. XXIV 85 90 78 81 Prod. of Ex. XXV 73 81 77 64 Prod. of Ex. XXVI 72 49 5 0 Prod. of Ex. XXVII 87 85 52 6 Prod. of Ex. XXVIII 74 - - Prod. of Ex. XXX 87 80 64 16 Prod. of Ex. XXXI 85 74 74 28 Prod. of Ex. XXXII 94 69 31 2 Prod. of Ex. XXXIII 81 64 22 4 Prod. of Ex. XXXIV 71 84 54 17 Example XLI The following spiro-hydantoin compounds of Preparation C and Examples I V, VII-IX, XI-XVII, XIX-XXV,XXVII and XXX-XXXIII, respectively, were tested for their ability to reduce or inhibit sorbitol accumulation in the sciatic nerve of streptozotocinized (i.e., diabetic) rats by the procedure essentially described in U.S. Patent No. 3,821,383. In the present study, the amount of sorbitol accumulation in the sciatic nerves was measured 27 hours after induction of diabetes. The compounds were administered orally at the dose levels indicated at 4, 8 and 24 hours following the administration of streptozotocin. The results obtained in this manner are presented below in terms of percent inhibition (%) afforded by the test compound as compared to the case where no compound was administered (i.e., the untreated anumal where sorbitol levels normally rise from approximately 50-100 mM/g. tissue to as high as 400 mM/g. tissue in the 27-hour test period).

Percent Inhibition (%) Compound 0.75 1.5 2.5 5.0 10 mg./kg.

Prod. of Prep. C - - - 3 40 Prod. of Ex. I - 29 - 52 67 Prod. of Ex. II - - - 6 54 Prod. of Ex. III - - - 45 Prod. of Ex. IV - - - 33 49 Prod. of Ex. V - - - 9 Prod. of Ex. VII - - - 39 65 Prod. of Ex. VIII - - - 26 Prod.of Ex. IX - - 58 - Prod. of Ex. XI - - - 59 Percent Inhibition (%) Compound 0.75 1.5 2.5 5.0 10 mg./kg.

Prod. of Ex. XII 13 45 74 - Prod. of Ex. XIII - 5 - - Prod. of Ex. XIV - 26 - - Prod. of Ex. XV - - - 5 52 Prod. of Ex. XVI - - - 25 44 Prod. of Ex. XVII - - - - 3 Prod. of Ex. XIX - - - - 15 Prod. of Ex. XX - 34 58 77 Prod. of Ex. XXI - - 24 - Prod. of Ex. XXII 45 72 - - Prod. of Ex. XXIII - 64 - - Prod. of Ex. XXIV 82 - - - - Prod. of XXV 64 84 - - Prod. of Ex. XXVII - 30 - - Prod. of Ex. XXX - 35 - - Prod. of Ex. XXXI - - 47 68 Prod. of Ex. XXXII 28 - 12 - Prod. of Ex. XXXIII - - - 5 Example XLII 6 - Fluoro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione (the product of Example XXII) was tested for its ability to reverse already-elevated sorbitol levels in streptozotocin-induced diabetic rats of two weeks duration (i.e., chronic) by administering said compound orally to the animals for a period of seven days. In this study, the sorbitol detcrminations were carried out in both the sciatic nerve and the lens. Streptozotocin was first administered to the animals at 65 mg./kg., via the intravenous route. The animals then remained untreated for a period of two weeks. At the end of this time, a "control" group of eight rats (Control Group I) was sacrificed for base-line sorbitol determinations, while the remaining two groups of seven animals each either received 6 - fluoro - spiro [chroman - 4,4' - imidazolidine] - 2',5' - dione at 2.5 mg./kg., twice a day, or simply water alone (Control Group II). After seven days, the rats were sacrificed (three hours post dose) and it was found that while sciatic nerve sorbitol levels in the control group (Control Group II) had risen slightly above baseline values and lens sorbitol values had stabilized with respect to same, substantial reductions in sorbitol levels had occurred in both the sciatic nerve (68%) and lens (71%) of the treated group (i.e., those animals receiving the aforesaid test compound).

Example XLIII The ability of 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' dione to prevent or inhibit galactitol formation in acutely galactosemic rats was determined by administering said compound to the animals, via their feed, for a period of seven days. In this study, normal male rats were first divided into groups of six animals each and then fed a 30% galactose diet together with the compound to be administered at three different dosage levels. One group of animals received 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione at 10 mg./kg. and another at 20 mg./kg., respectively. A control group of nine animals received a 30% galactose diet without any compound. At the end of the seven-day period, lenses were removed for galactitol determination and it was found that while polyol levels in the control group had risen from essentially undetectable amounts to a value of well over 30 moles/g., in those rats receiving the test compound in the diet in addition to galactose, there was definitely a very pronounced inhibition of galactitol values at the two higher dose levels tested (e.g., 72% at 20 mg./kg. and 40% at 10 mg./l:g. respectively).

Example XLIV To determine the effect of 6 - fluoro - spiro - [chroman - 4,4' imidazolidine] - 2'5' - dione on cataract formation in galactosemia, rats were fed a 30% galactose diet with and without this compound for a period of 29 days and eye examinations also were routinely conducted approximately twice a week throughout this period. The experimental animals received the test compound mixed in the food at concentration levels necessary to afford approximate doses of 10 mg./kg. and 20 mg./kg., respectively. Control animals received the galactose diet alone (i.e., without the compound). After 8-14 days, it was found that lenticular opacities had developed in 909d of the eyes of the control animals as compared to no opacities being present in the cases of those rats receiving 6 - fluoro - spiro [chroman - 4,4' - imidazolidine] - 2',5' - dione at either 10 mg./kg. or 20 mg./kg., as aforesaid. At the end of 17 days, it was found that opacities were present in 1000, of the eyes of the control animals. while only 60.,, of the eyes of those rats receiving 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione at 10 m,e./kg. were actually affected. The corresponding value obtained in rats receiving the test compound at 20 mg./kg. was 0%. This delay in cataract formation continued in all the treated groups until the 22-day mark, at which point lenticular opacities were observed in greater than 90 , of the eyes of those animals receiving the test compound at the 10 mg./kg. dose level. However, in rats receiving 6 - fluoro spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione at 20 mg./kg., an impressive delay in cataract formation was still observed at the 29-day mark, as evidenced by the fact that only 37% of the eyes of the animals in the treated group showed lenticular opacities.

Example XLV The effectiveness of 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] 2',5' - dione in delaying cataract development in rats is further highlighted by the test procedure of Example XLIV by giving careful consideration to the severity of the lenticular opacities involved. In this study, the percentage of lens areas involved were monitored throughout the 29 day period and the results obtained served as an index of severity. In this way, it was found that after 17 days, 75% of the control lenses involved showed no area of involvement which was never less than 10%. On the other hand, corresponding values of 6% and 0% were respectively obtained in the case of those rats receiving 6 - fluoro - spiro - [chroman - 4,4' imidazolidine] - 2',5' - dione at 10 mg./kg. and 20 mg./kg. dose levels. As a matter of fact, the severity of lenticular opacities in the treated groups was always less than that found in the control group, including the values obtained at the end of the 29day mark.

Example XLVI The compounds prepared in Example XXXV are subjected to the test procedure of Example XL and are active as aldose reductase inhibitors at doses corresponding to at least one of the concentration levels previously indicated.

Claims

WHAT WE CLAIM IS:

1. A method of treating a non-human diabetic host to prevent or alleviate diabetes-associated chronic complications, which comprises administering to said host an effective amount of a compound selected from the group consisting of those of the formulae:

and the base salts thereof with pharmacologically acceptable cations, wherein W is CH2)0-; X is hydrogen and X' is hydrogen, hydroxy, fluorine, chlorine bromine, C1-C4 alkyl or C1-C4 alkoxy; or X and X', when taken separately, are each chlorine, C1-C4 alkyl or C1-C4 alkoxy and when taken together are -OCH2(CH2)O-; Y is oxygen or sulfur; Z is W, Y or Q wherein Q is

and n is zero or one.

2. The method as claimed in Claim 1 wherein the compound administered is spiro - [imidazolidine - 4,1' - indan] - 2,5 - dione.

3. The method as claimed in Claim 1, wherein the compound administered is 6 - fluoro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione.

4. The method as claimed in Claim 1, wherein the compound administered is 6 - chloro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione.

5. The method as claimed in Claim 1, wherein the compound administered is 6,8 dichloro - spiro - [chroman - 4,4' - imidazolidinel - 2',5' - dione.

6. A compound of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein W is HCH2)n, and n Is zero or one.

7. A compound as claimed in Claim 6, wherein n is zero.

8. A compound as claimed in Claim 6, wherein n is one.

9. A compound of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein X is hydrogen and X2 is fluorine, hydroxy or 6'-(C1-C4 alkoxy); or X and X2, when taken separately, are each C1-C4 alkoxy, and when taken together are -OCH2(CH2)O-; and n is zero or one.

10. A compound as claimed in Claim 9, wherein X is hydrogen and X2 is fluorine.

I I. A compound as claimed in Claim 9, wherein X is hydrogen and X2 is 6f- methoxy.

12. A compound of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein X is hydrogen and X4 is fluorine, chlorine or bromine: or X3 and X4, when taken separately, are each chlorine and when taken together are -OCH2(CH2)O-; and n is zero or one.

13. A compound of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein X5 is hydrogen and X6 is fluorine, hydroxy or C1-C4 alkoxy; or X5 and X8, when taken separately, are each chlorine or C1-C4 alkoxy, and when taken together are -OCH2(CH2)O-; Y is oxygen or sulfur; and n is zero or one.

14. A compound as claimed in Claim 13, wherein X5 is hydrogen, X6 is fluorine and Y is oxygen.

15. A compound as claimed in Claim 13, wherein X8 is hydrogen, X6 is lower alkoxy and Y is oxygen.

16. A compound as claimed in Claim 13, wherein X5 is hydrogen, X6 is fluorine and Y is sulfur.

17. A compound as claimed in Claim 13, wherein X8 is hydrogen, X6 is lower alkoxy and Y is sulfur.

18. A compound as claimed in Claim 13, wherein X5 and X6, when taken separately, are each chlorine and Y is oxygen.

19. A compound as claimed in Claim 13, wherein X5 and X6, when taken separately, are each chlorine and Y is sulfur.

20. A compound of the formula:

and the base salts thereof with pharmacologically acceptable cations, wherein X7 is hydrogen and X8 is hydrogen fluorine, chlorine, bromine or C1-C4 alkoxy; or X7 and X8, when taken separately, are each chlorine or C1-C4 alkoxy, and when taken together are -OCH2(CH2)O-; and O is

and n is zero or one.

21. A compound as claimed in Claim 20, wherein X7 is hydrogen, x8 is fluorine and Q is

22. A compound as claimed in Claim 20, wherein X7 is hydrogen, x8 is lower alkoxy and Q is

23. A compound as claimed in Claim 20, wherein X7 and X8 are each hydrogen and Q is

24. A compound as claimed in Claim 20, wherein X7 is hydrogen, X8 is fluorine and Q is

25. 6 - Fluoro - spiro - [chroman - 4,4' - imidazolidinel - 2',5' - dione.

26. 6,7 - Dichloro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione.

27. 6,8 - Dichloro - spiro - [chroman - 4,4' - imidazolidine] - 2',5' - dione.

28. 6' - Fluoro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 - dione.

29. 6',7' - Dichloro - spiro - [imidazolidine - 4,4' - thiochroman] - 2,5 dione.