GB1007146A - Improvements in or relating to the production of insulin - Google Patents

Improvements in or relating to the production of insulin

Info

Publication number
GB1007146A
GB1007146A GB3575661A GB3575661A GB1007146A GB 1007146 A GB1007146 A GB 1007146A GB 3575661 A GB3575661 A GB 3575661A GB 3575661 A GB3575661 A GB 3575661A GB 1007146 A GB1007146 A GB 1007146A
Authority
GB
United Kingdom
Prior art keywords
insulin
adjusted
acid
extract
pancreas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3575661A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Philips NV
Original Assignee
Philips Gloeilampenfabrieken NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philips Gloeilampenfabrieken NV filed Critical Philips Gloeilampenfabrieken NV
Publication of GB1007146A publication Critical patent/GB1007146A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C07K14/625—Extraction from natural sources

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Insulin is obtained from a pancreas extract prepared directly from ground pancreas tissue by extraction with an acidified aqueous extraction liquid containing an organic solvent, with or without intermediate removal in known manner of fatty constituents from said extract, by adding metal ions of zinc, cadmium, iron, nickel, cobalt, copper or manganese, separating off and then dissolving the separated protein fraction in a dilute aqueous acid and then recovering the insulin from the solution so formed. In one example, cow pancreas is extracted with alcohol containing hydrochloric, acid at pH 2.5, the pH of the liquid is adjusted to 7.5 to 8.5 with ammonia and the liquid is clarified, the pH is then adjusted to 2.5 with hydrochloric acid and an equal quantity of water added to precipitate fatty substances which are separated. Zinc chloride is added and the pH is adjusted to 6.0 with ammonia to precipitate the insulin and other proteins. After dissolution in hycrochloric acid of pH 2.5-3.0, insulin is recovered therefrom by known methods. Preferably the metal ions are added in the form of a salt in which the metal is present in its bivalent form and the pH of the extract is adjusted to 5.0 to 7.5. Specifications 566,351 and 726,219 are referred to.
GB3575661A 1960-10-07 1961-10-04 Improvements in or relating to the production of insulin Expired GB1007146A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL256652 1960-10-07

Publications (1)

Publication Number Publication Date
GB1007146A true GB1007146A (en) 1965-10-13

Family

ID=19752615

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3575661A Expired GB1007146A (en) 1960-10-07 1961-10-04 Improvements in or relating to the production of insulin

Country Status (2)

Country Link
ES (1) ES270952A1 (en)
GB (1) GB1007146A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2383901A1 (en) 2010-04-29 2011-11-02 Gigle Networks SL Wireless network devices configurable via powerline communications networks

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2383901A1 (en) 2010-04-29 2011-11-02 Gigle Networks SL Wireless network devices configurable via powerline communications networks

Also Published As

Publication number Publication date
DE1492044A1 (en) 1969-02-20
ES270952A1 (en) 1962-03-16

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