ES270952A1 - Improvements in or relating to the production of insulin - Google Patents

Improvements in or relating to the production of insulin

Info

Publication number
ES270952A1
ES270952A1 ES0270952A ES270952A ES270952A1 ES 270952 A1 ES270952 A1 ES 270952A1 ES 0270952 A ES0270952 A ES 0270952A ES 270952 A ES270952 A ES 270952A ES 270952 A1 ES270952 A1 ES 270952A1
Authority
ES
Spain
Prior art keywords
insulin
adjusted
acid
extract
pancreas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES0270952A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Philips NV
Original Assignee
Philips Gloeilampenfabrieken NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philips Gloeilampenfabrieken NV filed Critical Philips Gloeilampenfabrieken NV
Publication of ES270952A1 publication Critical patent/ES270952A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C07K14/625—Extraction from natural sources

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Insulin is obtained from a pancreas extract prepared directly from ground pancreas tissue by extraction with an acidified aqueous extraction liquid containing an organic solvent, with or without intermediate removal in known manner of fatty constituents from said extract, by adding metal ions of zinc, cadmium, iron, nickel, cobalt, copper or manganese, separating off and then dissolving the separated protein fraction in a dilute aqueous acid and then recovering the insulin from the solution so formed. In one example, cow pancreas is extracted with alcohol containing hydrochloric, acid at pH 2.5, the pH of the liquid is adjusted to 7.5 to 8.5 with ammonia and the liquid is clarified, the pH is then adjusted to 2.5 with hydrochloric acid and an equal quantity of water added to precipitate fatty substances which are separated. Zinc chloride is added and the pH is adjusted to 6.0 with ammonia to precipitate the insulin and other proteins. After dissolution in hycrochloric acid of pH 2.5-3.0, insulin is recovered therefrom by known methods. Preferably the metal ions are added in the form of a salt in which the metal is present in its bivalent form and the pH of the extract is adjusted to 5.0 to 7.5. Specifications 566,351 and 726,219 are referred to.
ES0270952A 1960-10-07 1961-10-05 Improvements in or relating to the production of insulin Expired ES270952A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL256652 1960-10-07

Publications (1)

Publication Number Publication Date
ES270952A1 true ES270952A1 (en) 1962-03-16

Family

ID=19752615

Family Applications (1)

Application Number Title Priority Date Filing Date
ES0270952A Expired ES270952A1 (en) 1960-10-07 1961-10-05 Improvements in or relating to the production of insulin

Country Status (2)

Country Link
ES (1) ES270952A1 (en)
GB (1) GB1007146A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201007146D0 (en) 2010-04-29 2010-06-09 Gigle Networks Sl Communications apparatus

Also Published As

Publication number Publication date
DE1492044A1 (en) 1969-02-20
GB1007146A (en) 1965-10-13

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