FR2485740A1 - Procede permettant d'ameliorer la sensibilite d'un lysat d'amibocytes de limule (lal) vis-a-vis de l'endotoxine, reactif lal correspondant, et utilisation de ce reactif pour determiner la presence d'endotoxines - Google Patents
Procede permettant d'ameliorer la sensibilite d'un lysat d'amibocytes de limule (lal) vis-a-vis de l'endotoxine, reactif lal correspondant, et utilisation de ce reactif pour determiner la presence d'endotoxines Download PDFInfo
- Publication number
- FR2485740A1 FR2485740A1 FR8112598A FR8112598A FR2485740A1 FR 2485740 A1 FR2485740 A1 FR 2485740A1 FR 8112598 A FR8112598 A FR 8112598A FR 8112598 A FR8112598 A FR 8112598A FR 2485740 A1 FR2485740 A1 FR 2485740A1
- Authority
- FR
- France
- Prior art keywords
- radical
- lysate
- sensitivity
- carbon atoms
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 19
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- 230000035945 sensitivity Effects 0.000 claims abstract description 66
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 44
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- FQEONVSBIOOXJN-UHFFFAOYSA-N bis(2-hydroxyethyl)azanium;octyl sulfate Chemical compound OCCNCCO.CCCCCCCCOS(O)(=O)=O FQEONVSBIOOXJN-UHFFFAOYSA-N 0.000 description 1
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- 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- KETHQOOVMIVLCH-UHFFFAOYSA-N hydron;nonyl sulfate Chemical compound CCCCCCCCCOS(O)(=O)=O KETHQOOVMIVLCH-UHFFFAOYSA-N 0.000 description 1
- RNYJXPUAFDFIQJ-UHFFFAOYSA-N hydron;octadecan-1-amine;chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[NH3+] RNYJXPUAFDFIQJ-UHFFFAOYSA-N 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- POIZGMCHYSVWDU-UHFFFAOYSA-N icosane-1,20-diamine Chemical compound NCCCCCCCCCCCCCCCCCCCCN POIZGMCHYSVWDU-UHFFFAOYSA-N 0.000 description 1
- MTNDZQHUAFNZQY-UHFFFAOYSA-N imidazoline Chemical compound C1CN=CN1 MTNDZQHUAFNZQY-UHFFFAOYSA-N 0.000 description 1
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
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- IZXGZAJMDLJLMF-UHFFFAOYSA-N methylaminomethanol Chemical compound CNCO IZXGZAJMDLJLMF-UHFFFAOYSA-N 0.000 description 1
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- HZAXFHJVJLSVMW-UHFFFAOYSA-N monoethanolamine hydrochloride Natural products NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
- 125000004573 morpholin-4-yl group Chemical group N1(CCOCC1)* 0.000 description 1
- 229940043348 myristyl alcohol Drugs 0.000 description 1
- QIBKKTQVKFEWCH-UHFFFAOYSA-N n'-tetradecylethane-1,2-diamine Chemical compound CCCCCCCCCCCCCCNCCN QIBKKTQVKFEWCH-UHFFFAOYSA-N 0.000 description 1
- 229940078490 n,n-dimethylglycine Drugs 0.000 description 1
- IBOBFGGLRNWLIL-UHFFFAOYSA-N n,n-dimethylhexadecan-1-amine oxide Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)[O-] IBOBFGGLRNWLIL-UHFFFAOYSA-N 0.000 description 1
- RSVIRMFSJVHWJV-UHFFFAOYSA-N n,n-dimethyloctan-1-amine oxide Chemical compound CCCCCCCC[N+](C)(C)[O-] RSVIRMFSJVHWJV-UHFFFAOYSA-N 0.000 description 1
- VHXSGTCOHZCUKB-UHFFFAOYSA-N n,n-dimethyltridecan-1-amine oxide Chemical compound CCCCCCCCCCCCC[N+](C)(C)[O-] VHXSGTCOHZCUKB-UHFFFAOYSA-N 0.000 description 1
- GOQYKNQRPGWPLP-UHFFFAOYSA-N n-heptadecyl alcohol Natural products CCCCCCCCCCCCCCCCCO GOQYKNQRPGWPLP-UHFFFAOYSA-N 0.000 description 1
- 125000000740 n-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- YMGXBOAPWTUMRD-UHFFFAOYSA-N n-propyltetradecan-1-amine Chemical compound CCCCCCCCCCCCCCNCCC YMGXBOAPWTUMRD-UHFFFAOYSA-N 0.000 description 1
- 125000001400 nonyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- SNQQPOLDUKLAAF-UHFFFAOYSA-N nonylphenol Chemical compound CCCCCCCCCC1=CC=CC=C1O SNQQPOLDUKLAAF-UHFFFAOYSA-N 0.000 description 1
- MXXDSLLVYZMTFA-UHFFFAOYSA-N octadecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 MXXDSLLVYZMTFA-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000006259 organic additive Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 239000001120 potassium sulphate Substances 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- KCXFHTAICRTXLI-UHFFFAOYSA-N propane-1-sulfonic acid Chemical compound CCCS(O)(=O)=O KCXFHTAICRTXLI-UHFFFAOYSA-N 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000001453 quaternary ammonium group Chemical group 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 239000013643 reference control Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- MZSDGDXXBZSFTG-UHFFFAOYSA-M sodium;benzenesulfonate Chemical compound [Na+].[O-]S(=O)(=O)C1=CC=CC=C1 MZSDGDXXBZSFTG-UHFFFAOYSA-M 0.000 description 1
- GGHPAKFFUZUEKL-UHFFFAOYSA-M sodium;hexadecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCCCCOS([O-])(=O)=O GGHPAKFFUZUEKL-UHFFFAOYSA-M 0.000 description 1
- FTWCSAMTIKSPAT-UHFFFAOYSA-M sodium;nonyl sulfate Chemical compound [Na+].CCCCCCCCCOS([O-])(=O)=O FTWCSAMTIKSPAT-UHFFFAOYSA-M 0.000 description 1
- NWZBFJYXRGSRGD-UHFFFAOYSA-M sodium;octadecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCCCCCCOS([O-])(=O)=O NWZBFJYXRGSRGD-UHFFFAOYSA-M 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 229940035044 sorbitan monolaurate Drugs 0.000 description 1
- 239000001593 sorbitan monooleate Substances 0.000 description 1
- 235000011069 sorbitan monooleate Nutrition 0.000 description 1
- 229940035049 sorbitan monooleate Drugs 0.000 description 1
- 235000011071 sorbitan monopalmitate Nutrition 0.000 description 1
- 239000001570 sorbitan monopalmitate Substances 0.000 description 1
- 229940031953 sorbitan monopalmitate Drugs 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- SFVFIFLLYFPGHH-UHFFFAOYSA-M stearalkonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 SFVFIFLLYFPGHH-UHFFFAOYSA-M 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000000020 sulfo group Chemical group O=S(=O)([*])O[H] 0.000 description 1
- 125000001273 sulfonato group Chemical group [O-]S(*)(=O)=O 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 125000004434 sulfur atom Chemical group 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Cosmetics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16345680A | 1980-06-27 | 1980-06-27 | |
US06/254,233 US4322217A (en) | 1980-06-27 | 1981-04-14 | Process for preparing Limulus lysate |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2485740A1 true FR2485740A1 (fr) | 1981-12-31 |
FR2485740B1 FR2485740B1 (en, 2012) | 1985-05-24 |
Family
ID=26859656
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR8112598A Granted FR2485740A1 (fr) | 1980-06-27 | 1981-06-26 | Procede permettant d'ameliorer la sensibilite d'un lysat d'amibocytes de limule (lal) vis-a-vis de l'endotoxine, reactif lal correspondant, et utilisation de ce reactif pour determiner la presence d'endotoxines |
Country Status (9)
Country | Link |
---|---|
US (1) | US4322217A (en, 2012) |
AU (1) | AU545395B2 (en, 2012) |
CH (1) | CH647871A5 (en, 2012) |
DE (1) | DE3125181A1 (en, 2012) |
FR (1) | FR2485740A1 (en, 2012) |
GB (1) | GB2080524B (en, 2012) |
IT (1) | IT1211070B (en, 2012) |
NL (1) | NL8103113A (en, 2012) |
SE (1) | SE447029B (en, 2012) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8827557D0 (en) * | 1988-11-25 | 1988-12-29 | Radiometer Corporate Dev Ltd | Process for handling horseshoe crab amoebocyte lysate |
US5002015A (en) * | 1988-12-09 | 1991-03-26 | Aerotrace Hydraulics, Inc. | Submerged treadmill system for exercising animals |
DE69029785T2 (de) * | 1989-10-30 | 1997-05-28 | Biowhittaker Inc | Kinetische Bestimmungsmethode für Endotoxin unter Verwendung von Limulus-Amoebocyten-Lysat und chromogenem Substrat |
JP2957251B2 (ja) * | 1990-09-28 | 1999-10-04 | 生化学工業株式会社 | エンドトキシンの測定法 |
DE69211973T2 (de) * | 1991-12-25 | 1996-12-19 | Maruha Corp | Reagenzien und Verfahren zur Bestimmung von Beta-Glucanen |
JP2737514B2 (ja) * | 1992-01-24 | 1998-04-08 | 和光純薬工業株式会社 | エンドトキシン測定用試料の前処理方法 |
CA2112776C (en) * | 1993-01-21 | 2002-11-12 | Masakazu Tsuchiya | Process for inhibiting activity of endotoxin |
JP3242733B2 (ja) * | 1993-02-26 | 2001-12-25 | 生化学工業株式会社 | エンドトキシン特異的測定剤 |
JP3429036B2 (ja) * | 1993-09-30 | 2003-07-22 | 生化学工業株式会社 | エンドトキシンの特異的測定剤 |
US5716834A (en) * | 1994-08-19 | 1998-02-10 | National University Of Singapore | Cloned factor C cDNA of the Singapore horseshoe crab, Carcinoscorpius rotundicauda and purification of factor C proenzyme |
US5637474A (en) * | 1994-10-31 | 1997-06-10 | Wako Pure Chemical Industries, Ltd. | Process for measuring amount of endotoxin or (1→3)-β-D-glucan by kinetic turbidimetric method |
US6270982B1 (en) | 1997-10-09 | 2001-08-07 | Charles River Laboratories | Methods and compositions for the detection of bacterial endotoxins |
ATE437886T1 (de) * | 2001-06-28 | 2009-08-15 | Lonza Walkersville Inc | Verfahren und reagentien zum nachweis von endotoxin |
US20040138577A1 (en) * | 2002-12-20 | 2004-07-15 | Kline Jeffrey A. | Disposable hand-held device for collection of exhaled breath condensate |
US7828741B2 (en) * | 2002-12-20 | 2010-11-09 | The Charlotte-Mecklenburg Hospital Authority | Utilizing lipopolysaccharide in exhaled breath condensate to diagnose gram negative pneumonia |
WO2004073497A2 (en) * | 2003-02-14 | 2004-09-02 | The Charlotte-Mecklenburg Hospital Authority | Device and method for collection of exhaled alveolar breath condensate |
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JP5437660B2 (ja) | 2009-02-19 | 2014-03-12 | 興和株式会社 | コアギュロゲン原料及びその製造方法、それを用いた生物由来の生理活性物質の測定方法及び測定装置 |
MY155541A (en) * | 2010-10-29 | 2015-10-30 | Universiti Malaysia Terengganu | Process for the preparation of amoebocyte lysate from the haemolymph of the horseshoe crab |
CU24073B1 (es) | 2011-12-27 | 2015-01-29 | Ct De Investigación Y Desarrollo De Medicamentos Cidem | COMPOSICIÓN A PARTIR DE EXTRACTO DE HEMOCITOS DE LANGOSTA PARA LA DETECCIÓN DE LIPOPOLISACÁRIDOS, PEPTIDOGLICANOS Y 1,3-ß-D-GLUCANOS |
EP3494226B1 (en) | 2016-08-03 | 2021-09-22 | Lonza Walkersville, Inc. | Method of detecting an endotoxin using limulus amebocyte lysate substantially free of coagulogen |
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FR1464280A (fr) * | 1964-12-28 | 1966-12-30 | Monsanto Co | Compositions d'adjuvants et détergents contenant ces compositions |
US3539521A (en) * | 1965-05-03 | 1970-11-10 | Procter & Gamble | Detergent composition |
FR2279105A1 (fr) * | 1974-07-17 | 1976-02-13 | Baxter Laboratories Inc | Lysat de limulus ayant une capacite accrue a precipiter en presence de faibles concentrations d'endotoxine, et solutions de reconstitution appropriees |
FR2438269A1 (fr) * | 1978-10-06 | 1980-04-30 | Baxter Travenol Lab | Procede optique de mesure de concentration d'endotoxines |
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US3280179A (en) * | 1961-03-16 | 1966-10-18 | Textilana Corp | Processes for producing acyclic surfactant sulfobetaines |
US3915805A (en) * | 1970-05-25 | 1975-10-28 | Univ Johns Hopkins | Quantitative detection of endotoxin in biological fluids |
US3954663A (en) * | 1972-12-18 | 1976-05-04 | Teikoku Horinine Mfg. Co. Ltd. | Process for the preparation of Limulina lysate |
US3944391A (en) | 1974-12-27 | 1976-03-16 | Preventive Systems, Inc. | In vitro process for detecting endotoxin in a biological fluid |
US4107077A (en) * | 1975-07-14 | 1978-08-15 | Associates Of Cape Cod, Inc. | Limulus lysate of improved sensitivity and preparing the same |
US4038029A (en) * | 1976-01-20 | 1977-07-26 | Worthington Biochemical Corporation | Limulus lysate turbidity test for pyrogens |
GB1522127A (en) | 1976-07-23 | 1978-08-23 | Mallinckrodt Inc | Limulus amebocyte lysate reagent compositions |
GB2019563B (en) | 1978-04-20 | 1982-07-28 | Harvard College | Assay of gram-negative bacteria |
US4273557A (en) * | 1980-03-31 | 1981-06-16 | Mallinckrodt, Inc. | Limulus lysate procedure for determining endotoxins |
-
1981
- 1981-04-14 US US06/254,233 patent/US4322217A/en not_active Expired - Lifetime
- 1981-06-23 GB GB8119357A patent/GB2080524B/en not_active Expired
- 1981-06-26 NL NL8103113A patent/NL8103113A/nl not_active IP Right Cessation
- 1981-06-26 SE SE8104016A patent/SE447029B/sv not_active IP Right Cessation
- 1981-06-26 CH CH4249/81A patent/CH647871A5/fr not_active IP Right Cessation
- 1981-06-26 IT IT8122595A patent/IT1211070B/it active
- 1981-06-26 AU AU72321/81A patent/AU545395B2/en not_active Expired
- 1981-06-26 DE DE19813125181 patent/DE3125181A1/de active Granted
- 1981-06-26 FR FR8112598A patent/FR2485740A1/fr active Granted
Patent Citations (4)
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FR1464280A (fr) * | 1964-12-28 | 1966-12-30 | Monsanto Co | Compositions d'adjuvants et détergents contenant ces compositions |
US3539521A (en) * | 1965-05-03 | 1970-11-10 | Procter & Gamble | Detergent composition |
FR2279105A1 (fr) * | 1974-07-17 | 1976-02-13 | Baxter Laboratories Inc | Lysat de limulus ayant une capacite accrue a precipiter en presence de faibles concentrations d'endotoxine, et solutions de reconstitution appropriees |
FR2438269A1 (fr) * | 1978-10-06 | 1980-04-30 | Baxter Travenol Lab | Procede optique de mesure de concentration d'endotoxines |
Non-Patent Citations (1)
Title |
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CHEMICAL ABSTRACTS, volume 84, no. 3, 19 janvier 1976, réf. 13096v, page 97, COLUMBUS, OHIO (US); & Yakugaku Zasshi 1975, 95(8), 959-65, M. KOBAYASHI et al.: "Gelation reaction of Limulus lysate (Pre-gel) IV. Effect of alkaline earth metals and chelating agents on the gelation reaction of Limulus lysate". * |
Also Published As
Publication number | Publication date |
---|---|
DE3125181C2 (en, 2012) | 1991-09-05 |
NL8103113A (nl) | 1982-01-18 |
SE447029B (sv) | 1986-10-20 |
SE8104016L (sv) | 1981-12-28 |
GB2080524B (en) | 1984-04-18 |
AU545395B2 (en) | 1985-07-11 |
DE3125181A1 (de) | 1982-03-04 |
IT1211070B (it) | 1989-09-29 |
CH647871A5 (fr) | 1985-02-15 |
NL192139B (en, 2012) | 1996-10-01 |
US4322217A (en) | 1982-03-30 |
FR2485740B1 (en, 2012) | 1985-05-24 |
IT8122595A0 (it) | 1981-06-26 |
GB2080524A (en) | 1982-02-03 |
AU7232181A (en) | 1982-01-07 |
NL192139C (en, 2012) | 1997-02-04 |
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