FR2367286A1 - Nouveau procede et reactifs de dosage immunochimique de substances physiologiquement actives - Google Patents
Nouveau procede et reactifs de dosage immunochimique de substances physiologiquement activesInfo
- Publication number
- FR2367286A1 FR2367286A1 FR7730219A FR7730219A FR2367286A1 FR 2367286 A1 FR2367286 A1 FR 2367286A1 FR 7730219 A FR7730219 A FR 7730219A FR 7730219 A FR7730219 A FR 7730219A FR 2367286 A1 FR2367286 A1 FR 2367286A1
- Authority
- FR
- France
- Prior art keywords
- substance
- immunochemical
- reagents
- dosage
- active substances
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000984 immunochemical effect Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000013543 active substance Substances 0.000 title 1
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 239000000126 substance Substances 0.000 abstract 6
- 238000003556 assay Methods 0.000 abstract 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 102000011022 Chorionic Gonadotropin Human genes 0.000 abstract 1
- 108010062540 Chorionic Gonadotropin Proteins 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108010026331 alpha-Fetoproteins Proteins 0.000 abstract 1
- 102000013529 alpha-Fetoproteins Human genes 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 229940084986 human chorionic gonadotropin Drugs 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/971—Capture of complex after antigen-antibody reaction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
- Y10S436/818—Human chorionic gonadotropin
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention a pour objet un procédé immunochimique de dosage, dans lequel on fait réagir une substance à doser tel qu'un antigène avec une substance insolubilisée, telle qu'un anticorps qui se lie spécifiquement à la substance à doser et sans séparer le produit de réaction du mélange, on fait réagir ce dernier avec une substance marquée qui se lie spécifiquement à la substance à doser. Ce procédé est applicable notamment au dosage d'alphafétoprotéine, d'insuline ou de gonadotropine chorionique humaine ou de substances physiologiques actives dans l'organisme humain.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP51120622A JPS607229B2 (ja) | 1976-10-07 | 1976-10-07 | 免疫化学的測定方法 |
JP12062176A JPS5347518A (en) | 1976-10-07 | 1976-10-07 | Immunologically measuring method |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2367286A1 true FR2367286A1 (fr) | 1978-05-05 |
FR2367286B1 FR2367286B1 (fr) | 1982-09-17 |
Family
ID=26458166
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7730219A Granted FR2367286A1 (fr) | 1976-10-07 | 1977-10-07 | Nouveau procede et reactifs de dosage immunochimique de substances physiologiquement actives |
Country Status (5)
Country | Link |
---|---|
US (1) | US4248965A (fr) |
DE (1) | DE2744836A1 (fr) |
FR (1) | FR2367286A1 (fr) |
GB (1) | GB1572220A (fr) |
NL (1) | NL178193C (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0023989A1 (fr) * | 1979-07-28 | 1981-02-18 | Medac Gesellschaft für klinische Spezialpräparate mbH | Essai immunoenzymatique pour la détection d'anticorps spécifiques aux pathogènes et trousse de réactifs pour sa mise en oeuvre |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5622871A (en) * | 1987-04-27 | 1997-04-22 | Unilever Patent Holdings B.V. | Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents |
AU531777B2 (en) * | 1978-04-05 | 1983-09-08 | Syva Co. | Label/solid conjugate immunoassay system |
JPS5539702A (en) * | 1978-08-30 | 1980-03-19 | Takeda Chem Ind Ltd | Method of measuring enzyme immunity of pancreas glucagon |
US4244940A (en) * | 1978-09-05 | 1981-01-13 | Bio-Rad Laboratories, Inc. | Single-incubation two-site immunoassay |
NL8000173A (nl) * | 1980-01-11 | 1981-08-03 | Akzo Nv | Toepassing van in water dispergeerbare, hydrofobe kleurstoffen als label in immunochemische testen. |
CA1160566A (fr) * | 1980-04-25 | 1984-01-17 | Harald Gallati | Methode de dosage immunologique |
US6406920B1 (en) | 1980-06-20 | 2002-06-18 | Inverness Medical Switzerland Gmbh | Processes and apparatus for carrying out specific binding assays |
US4879219A (en) * | 1980-09-19 | 1989-11-07 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
US4617260A (en) * | 1981-02-27 | 1986-10-14 | Merck & Co., Inc. | RIA assay for HBcAg |
JPS5856696A (ja) * | 1981-09-30 | 1983-04-04 | Amano Pharmaceut Co Ltd | カラムを用いる酵素免疫測定法 |
US4504585A (en) * | 1982-04-05 | 1985-03-12 | Aalto Scientific, Ltd. | Affinity immunoassay system |
US4522922A (en) * | 1982-04-16 | 1985-06-11 | Jose Carro | Soluble assay |
US4792528A (en) * | 1982-05-21 | 1988-12-20 | The Trustees Of Columbia University In The City Of New York | Methods for obtaining monoclonal antibodies useful in enhanced sensitivity immunoassays |
US4514505A (en) * | 1982-05-21 | 1985-04-30 | The Trustees Of Columbia University In The City Of New York | Monoclonal antibody mixtures and use thereof for enhanced sensitivity immunoassays |
US4471058A (en) * | 1982-07-26 | 1984-09-11 | Board Of Trustees Operating Michigan State University | Method for the detection and/or determination of a polyvalent antigen using at least two different monoclonal antibodies |
US4596771A (en) * | 1982-09-27 | 1986-06-24 | Research Corporation | Monoclonal antibodies to vitamin B-6 and immunossay method |
WO1984004598A1 (fr) * | 1983-05-06 | 1984-11-22 | Univ Columbia | Analyse immunologique de la gonadotropine chorionique humaine |
US4824784A (en) * | 1985-04-08 | 1989-04-25 | Hygeia Sciences, Incorporated | Chromogenic solution for immunoassay |
US4743542A (en) * | 1985-04-11 | 1988-05-10 | Ortho Diagnostic | Method for forestalling the hook effect in a multi-ligand immunoassay system |
US4871683A (en) * | 1985-04-18 | 1989-10-03 | Beckman Instruments, Inc. | Apparatus and method using a new reaction capsule |
US4931385A (en) * | 1985-06-24 | 1990-06-05 | Hygeia Sciences, Incorporated | Enzyme immunoassays and immunologic reagents |
US5236826A (en) * | 1985-12-10 | 1993-08-17 | Murex Corporation | Immunoassay for the detection or quantitation of an analyte |
US5501949A (en) * | 1985-12-10 | 1996-03-26 | Murex Diagnostics Corporation | Particle bound binding component immunoassay |
AU6839587A (en) * | 1985-12-10 | 1987-06-30 | Murex Corp. | Particle-bound binding component immunoassay |
US4868108A (en) * | 1985-12-12 | 1989-09-19 | Hygeia Sciences, Incorporated | Multiple-antibody detection of antigen |
DE3638767A1 (de) * | 1986-11-13 | 1988-05-26 | Behringwerke Ag | Laktoferrin enthaltendes inkubationsmedium fuer festphasenimmunometrisches verfahren und seine verwendung |
EP1248112A3 (fr) | 1987-04-27 | 2004-08-25 | Inverness Medical Switzerland GmbH | Dispositif d'essai immunochromatographique à liaison spécifique |
US4965191A (en) * | 1988-02-12 | 1990-10-23 | Eastman Kodak Company | Lower alcohol sulfate wash solution, test kit and method for the determination of an immunological ligand |
US5017474A (en) * | 1988-02-12 | 1991-05-21 | Eastman Kodak Company | Wash solution, test kit and method for the determination of an immunological ligand |
US5185128A (en) * | 1988-02-12 | 1993-02-09 | Eastman Kodak Company | Test kit containing labeled receptor and wash solution for determination of an immunological ligand |
US5256532A (en) * | 1988-05-02 | 1993-10-26 | Zynaxis Technologies, Inc. | Methods, reagents and test kits for determination of subpopulations of biological entities |
US5385822A (en) * | 1988-05-02 | 1995-01-31 | Zynaxis, Inc. | Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population |
US5102788A (en) * | 1988-11-21 | 1992-04-07 | Hygeia Sciences, Inc. | Immunoassay including lyophilized reactant mixture |
US6352862B1 (en) | 1989-02-17 | 2002-03-05 | Unilever Patent Holdings B.V. | Analytical test device for imuno assays and methods of using same |
US5869345A (en) * | 1991-05-29 | 1999-02-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing conductive barrier |
US6168956B1 (en) | 1991-05-29 | 2001-01-02 | Beckman Coulter, Inc. | Multiple component chromatographic assay device |
US5998220A (en) * | 1991-05-29 | 1999-12-07 | Beckman Coulter, Inc. | Opposable-element assay devices, kits, and methods employing them |
US5424219A (en) * | 1991-10-25 | 1995-06-13 | Cytech Biomedical, Inc. | Method of performing assays for biomolecules and solid supports for use in such methods |
US5354692A (en) * | 1992-09-08 | 1994-10-11 | Pacific Biotech, Inc. | Analyte detection device including a hydrophobic barrier for improved fluid flow |
US5879951A (en) * | 1997-01-29 | 1999-03-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing unidirectional flow |
DK2268669T3 (da) * | 2008-03-13 | 2017-11-20 | Immuron Ltd | Bovin kolostrum omfattende antistoffer mod insulin til behandling af stofskifteforstyrrelsessygdomme |
FR2960974B1 (fr) * | 2010-06-03 | 2016-12-30 | Biosynex | Procede de detection de rupture de membranes |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2164705A1 (fr) * | 1971-12-21 | 1973-08-03 | Abbott Lab | |
US3791932A (en) * | 1971-02-10 | 1974-02-12 | Akzona Inc | Process for the demonstration and determination of reaction components having specific binding affinity for each other |
US3850752A (en) * | 1970-11-10 | 1974-11-26 | Akzona Inc | Process for the demonstration and determination of low molecular compounds and of proteins capable of binding these compounds specifically |
US4016043A (en) * | 1975-09-04 | 1977-04-05 | Akzona Incorporated | Enzymatic immunological method for the determination of antigens and antibodies |
US4038378A (en) * | 1975-12-22 | 1977-07-26 | Gyaneshwar Prasad Khare | Radioimmunoassay for hepatitis b antigen |
FR2362397A1 (fr) * | 1976-08-20 | 1978-03-17 | Immuno Ag | Procede radio-immunologique et dispositif servant a determiner les antigenes |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3654090A (en) * | 1968-09-24 | 1972-04-04 | Organon | Method for the determination of antigens and antibodies |
US4067959A (en) * | 1976-05-10 | 1978-01-10 | International Diagnostic Technology, Inc. | Indirect solid surface test for antigens or antibodies |
-
1977
- 1977-09-28 GB GB40344/77A patent/GB1572220A/en not_active Expired
- 1977-10-03 US US05/838,947 patent/US4248965A/en not_active Expired - Lifetime
- 1977-10-05 DE DE19772744836 patent/DE2744836A1/de active Pending
- 1977-10-06 NL NLAANVRAGE7710980,A patent/NL178193C/xx not_active IP Right Cessation
- 1977-10-07 FR FR7730219A patent/FR2367286A1/fr active Granted
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3850752A (en) * | 1970-11-10 | 1974-11-26 | Akzona Inc | Process for the demonstration and determination of low molecular compounds and of proteins capable of binding these compounds specifically |
US3791932A (en) * | 1971-02-10 | 1974-02-12 | Akzona Inc | Process for the demonstration and determination of reaction components having specific binding affinity for each other |
FR2164705A1 (fr) * | 1971-12-21 | 1973-08-03 | Abbott Lab | |
US4016043A (en) * | 1975-09-04 | 1977-04-05 | Akzona Incorporated | Enzymatic immunological method for the determination of antigens and antibodies |
US4038378A (en) * | 1975-12-22 | 1977-07-26 | Gyaneshwar Prasad Khare | Radioimmunoassay for hepatitis b antigen |
FR2362397A1 (fr) * | 1976-08-20 | 1978-03-17 | Immuno Ag | Procede radio-immunologique et dispositif servant a determiner les antigenes |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0023989A1 (fr) * | 1979-07-28 | 1981-02-18 | Medac Gesellschaft für klinische Spezialpräparate mbH | Essai immunoenzymatique pour la détection d'anticorps spécifiques aux pathogènes et trousse de réactifs pour sa mise en oeuvre |
Also Published As
Publication number | Publication date |
---|---|
GB1572220A (en) | 1980-07-30 |
FR2367286B1 (fr) | 1982-09-17 |
DE2744836A1 (de) | 1978-04-13 |
NL178193B (nl) | 1985-09-02 |
NL7710980A (nl) | 1978-04-11 |
US4248965A (en) | 1981-02-03 |
NL178193C (nl) | 1986-02-03 |
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