FI96433B - Monoklonala antikroppar för selektiv, immunologisk bestämning av intakt prokollagenpeptid (typ III) och prokollagen (typ III) från kroppsvätskor - Google Patents

Monoklonala antikroppar för selektiv, immunologisk bestämning av intakt prokollagenpeptid (typ III) och prokollagen (typ III) från kroppsvätskor Download PDF

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Publication number
FI96433B
FI96433B FI882019A FI882019A FI96433B FI 96433 B FI96433 B FI 96433B FI 882019 A FI882019 A FI 882019A FI 882019 A FI882019 A FI 882019A FI 96433 B FI96433 B FI 96433B
Authority
FI
Finland
Prior art keywords
type iii
procollagen
procollagen peptide
peptide
monoclonal antibody
Prior art date
Application number
FI882019A
Other languages
English (en)
Finnish (fi)
Other versions
FI882019A0 (sv
FI882019A (sv
FI96433C (sv
Inventor
Dietrich Brocks
Rupert Timpl
Helmut Strecker
Juergen Puenter
Original Assignee
Hoechst Ag
Max Planck Gesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag, Max Planck Gesellschaft filed Critical Hoechst Ag
Publication of FI882019A0 publication Critical patent/FI882019A0/sv
Publication of FI882019A publication Critical patent/FI882019A/sv
Publication of FI96433B publication Critical patent/FI96433B/sv
Application granted granted Critical
Publication of FI96433C publication Critical patent/FI96433C/sv

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in eptitope analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6887—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from muscle, cartilage or connective tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Separation By Low-Temperature Treatments (AREA)
  • Pressure Welding/Diffusion-Bonding (AREA)

Claims (7)

1. Monoklonal antikropp, kännetecknad av att den kan binda sig specifikt med intakt prokollagen 5 (typ III), pN-kollagen och intakt aminoterminal prokolla- genpeptid (typ III), men varken med Col 1 eller med sädana sönderfallsprodukter av aminoterminal prokollagenpeptid (typ III) som har samma molekylvikt som Col 1, att den har egenskaperna av en monoklonal antikropp producerad med 10 hybridomcellinjen ECACC 87 042 308 och att den kan produ-ceras genom att odla en hybridom, som har bildats som re-sultat av en fusion mellan celler av en myelomlinje och lymfocyter frän ett i förväg med prokollagenpeptid (typ III) immuniserat djur, och genom att tillvarata de bildade 15 antikropparna.
2. Monoklonal antikropp enligt patentkrav 1, kännetecknad av, att den hör till IgG-klassen.
3. Monoklonal antikropp enligt patentkrav 1, k ä n-netecknad av, att den är en monoklonal antikropp
20. III P 296 producerad av en hybridomacellinje ECACC 87 042 308.
4. Hybridomcellinjen ECACC 87 042 308.
5. Förfarande för framställning av en monoklonal antikropp mot aminoterminal prokollagenpeptid (typ III), 25 kännetecknat av, att man a) odlar hybridomcellinjen ECACC 87 042 308 och b) tillvaratar de bildade antikropparna.
6. Förfarande för kvantitativ immunologisk bestäm-ning av prokollagenpeptiden (tyyppi III) med hjälp av an- 30 tikroppar, kännetecknat av, att • a) ett vätskeprov, som innehäller aminoterminal prokollagenpeptid (typ III) eller prokollagen (typ III), bringas att reagera med en monoklonal antikropp enligt nägot av patentkraven 1-3 och 15 96433 b) mängden av aminoterminal prokollagenpeptid (typ III) eller prokollagen bestäms pä basis av det antigen-antikroppkomplex som bildats.
7. Diagnostiskt preparat för detektering av mängden 5 av prokollagenpeptid (typ III) i kroppsvätskor, k ä n -netecknat av, att det innehäller en effektiv mängd av monoklonal antikropp enligt nägot av patentkraven 1 - 3 i en blandning med en diagnostiskt godtagbar bärare. <
FI882019A 1987-05-02 1988-04-29 Monoklonala antikroppar för selektiv, immunologisk bestämning av intakt prokollagenpeptid (typ III) och prokollagen (typ III) från kroppsvätskor FI96433C (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3714633 1987-05-02
DE3714633 1987-05-02

Publications (4)

Publication Number Publication Date
FI882019A0 FI882019A0 (sv) 1988-04-29
FI882019A FI882019A (sv) 1988-11-03
FI96433B true FI96433B (sv) 1996-03-15
FI96433C FI96433C (sv) 1996-06-25

Family

ID=6326685

Family Applications (1)

Application Number Title Priority Date Filing Date
FI882019A FI96433C (sv) 1987-05-02 1988-04-29 Monoklonala antikroppar för selektiv, immunologisk bestämning av intakt prokollagenpeptid (typ III) och prokollagen (typ III) från kroppsvätskor

Country Status (10)

Country Link
EP (1) EP0289930B1 (sv)
JP (1) JPH07110879B2 (sv)
AT (1) ATE89862T1 (sv)
DE (1) DE3881275D1 (sv)
DK (1) DK169583B1 (sv)
ES (1) ES2056850T3 (sv)
FI (1) FI96433C (sv)
IE (1) IE63371B1 (sv)
NO (1) NO173104C (sv)
PT (1) PT87376B (sv)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5679583A (en) * 1987-05-02 1997-10-21 Hoechst Aktiengesellschaft Monoclonal antibodies for the selective immunological determination of intact procollagen peptide (type III) and procollagen (type III) in body fluids
GB2208865B (en) * 1987-08-19 1991-12-11 Farmos Group Ltd Purified propeptide of procollagen type iii, antibody to the propeptide and assay method using the antibody.
US6010862A (en) * 1987-11-06 2000-01-04 Washington Research Foundation Methods of detecting collagen type III degradation in vivo
DE69915059T2 (de) * 1998-05-28 2004-08-26 Bayer Healthcare Ag Monoklonaler antikörper und assay zur bestimmung von n-terminalem prokollagen-propeptid typ iii (piiinp)
US6602980B1 (en) 1998-06-19 2003-08-05 Washington Research Foundation Collagen type III synthetic peptides for collagen resorption assays
US6916903B2 (en) 1998-06-19 2005-07-12 Washington Research Foundation Collagen type III synthetic peptides for collagen resorption assays

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3209149A1 (de) * 1982-03-13 1983-10-06 Hoechst Ag Verfahren zur gemeinsamen immunologischen bestimmung von prokollagen-peptid (typ iii) und prokollagen-peptid col 1 (typ iii) und verfahren zur herstellung von anti-prokollagen-peptid col 1 (typ iii)-serum

Also Published As

Publication number Publication date
PT87376B (pt) 1992-08-31
DK232888A (da) 1988-11-03
FI882019A0 (sv) 1988-04-29
DE3881275D1 (de) 1993-07-01
EP0289930B1 (de) 1993-05-26
IE63371B1 (en) 1995-04-19
EP0289930A2 (de) 1988-11-09
NO173104B (no) 1993-07-19
NO173104C (no) 1993-10-27
FI882019A (sv) 1988-11-03
JPH07110879B2 (ja) 1995-11-29
IE881290L (en) 1988-11-02
NO881905L (no) 1988-11-03
ES2056850T3 (es) 1994-10-16
ATE89862T1 (de) 1993-06-15
DK169583B1 (da) 1994-12-12
FI96433C (sv) 1996-06-25
EP0289930A3 (de) 1991-06-12
NO881905D0 (no) 1988-04-29
JPS63296695A (ja) 1988-12-02
PT87376A (pt) 1989-05-31
DK232888D0 (da) 1988-04-28

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Owner name: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENS

Owner name: HOECHST AKTIENGESELLSCHAFT