FI942644A - Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin - Google Patents

Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin Download PDF

Info

Publication number
FI942644A
FI942644A FI942644A FI942644A FI942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A
Authority
FI
Finland
Prior art keywords
cellulase
yeast
procedure
humicola insolence
cloning proteins
Prior art date
Application number
FI942644A
Other languages
English (en)
Swedish (sv)
Other versions
FI942644A0 (fi
Inventor
Henrik Dalboege
Hans Peter Heldt-Hansen
Grethe Rasmussen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of FI942644A publication Critical patent/FI942644A/fi
Publication of FI942644A0 publication Critical patent/FI942644A0/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
FI942644A 1991-12-04 1994-06-03 Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin FI942644A0 (fi)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK9100379 1991-12-04
DK9100378 1991-12-04
PCT/DK1992/000360 WO1993011249A1 (en) 1991-12-04 1992-12-02 A method of cloning proteins in yeast and cellulase from humicola insolens

Publications (2)

Publication Number Publication Date
FI942644A true FI942644A (fi) 1994-06-03
FI942644A0 FI942644A0 (fi) 1994-06-03

Family

ID=26068344

Family Applications (1)

Application Number Title Priority Date Filing Date
FI942644A FI942644A0 (fi) 1991-12-04 1994-06-03 Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin

Country Status (7)

Country Link
EP (1) EP0618974A1 (fi)
JP (1) JPH08504560A (fi)
BR (1) BR9206866A (fi)
CA (1) CA2124939A1 (fi)
FI (1) FI942644A0 (fi)
MX (1) MX9206979A (fi)
WO (1) WO1993011249A1 (fi)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69535733T2 (de) * 1994-10-06 2009-04-23 Novozymes A/S Ein enzympräparat mit endoglucanase aktivität
WO1996013580A1 (en) * 1994-10-26 1996-05-09 Novo Nordisk A/S An enzyme with lipolytic activity
ATE315083T1 (de) 1995-03-17 2006-02-15 Novozymes As Neue endoglukanase
US6410701B1 (en) * 1995-05-05 2002-06-25 Human Genome Sciences, Inc. Human neuropeptide receptor
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
AU7294396A (en) * 1995-10-13 1997-04-30 Gist-Brocades B.V. Protein detection
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
AR005601A1 (es) * 1996-01-29 1999-06-23 Novozymes As Proceso para la remocion o blanqueo de suciedad o manchas presentes en telas celulosicas y proceso para el lavado de telas celulosicas manchadas o sucias y composicion detergente
EP0877799A1 (en) * 1996-01-29 1998-11-18 Novo Nordisk A/S Process for desizing cellulosic fabric
DE69738254T2 (de) 1996-05-10 2008-08-14 Novozymes A/S Methode zur bereitstellung von dna sequenzen
AU2887897A (en) 1996-05-21 1997-12-09 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
DE69738158T2 (de) 1996-07-05 2008-06-19 Novozymes A/S Alpha-Amylase Transkriptionsfaktor
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
EP0975745B1 (en) * 1996-10-28 2008-02-20 Novozymes A/S Extracellular expression of cellulose binding domains (cbd) using bacillus
DE69716711T3 (de) 1996-12-09 2010-06-10 Novozymes A/S Verringerung von phosphorhaltigen Substanzen in Speiseölen mit hohem Anteil an nicht-hydratisierbarem Phosphor unter Verwendung einer Phospholipase, eine Phospholipase aus fädenförmigen Pilz, welche eine Phospholipase A und/oder B-Aktivität aufweist
DK1042461T3 (da) * 1997-12-22 2007-09-24 Dsm Ip Assets Bv Ekspressionskloning i filiforme svampe
WO1999045143A2 (en) * 1998-03-06 1999-09-10 Novo Nordisk A/S A fluorescence polarization screening method
CN101024826B (zh) 1998-06-10 2014-09-03 诺沃奇梅兹有限公司 新的甘露聚糖酶
US6723504B1 (en) 1998-10-28 2004-04-20 Novozymes A/S Method for generating a gene library
JP2002528075A (ja) * 1998-10-28 2002-09-03 ノボザイムス アクティーゼルスカブ 遺伝子ライブラリーの生成方法
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
EP1059351A1 (en) * 1999-06-11 2000-12-13 The Procter & Gamble Company Non-aqueous liquid detergent compositions comprising a borate-releasing compound and a mannanase
KR20020026456A (ko) 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝
IL146935A0 (en) * 2000-04-13 2002-08-14 Emalfarb Mark Aaron High-throughput screening of expressed dna libraries in filamentous fungi
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
DK1713825T3 (da) * 2004-01-30 2012-11-05 Novozymes Inc Polypeptider med cellulolytisk forøgende aktivitet og polynukleotider der koder for samme
WO2006008508A1 (en) 2004-07-16 2006-01-26 Danisco A/S Enzymatic oil-degumming method
BRPI0708949B1 (pt) 2006-03-20 2017-04-04 Novozymes As métodos para degradar ou converter um material celulósico, para produzir uma susbtância, e para produzir um polipeptídeo tendo atividade endoglucanase, e, célula microbiana recombinante
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
WO1989009259A1 (en) * 1988-03-24 1989-10-05 Novo-Nordisk A/S A cellulase preparation
AU639570B2 (en) * 1990-05-09 1993-07-29 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
ES2080957T3 (es) * 1990-07-13 1996-02-16 Transkaryotic Therapies Inc Metodo para escrutar una genoteca.

Also Published As

Publication number Publication date
BR9206866A (pt) 1995-11-21
JPH08504560A (ja) 1996-05-21
WO1993011249A1 (en) 1993-06-10
EP0618974A1 (en) 1994-10-12
CA2124939A1 (en) 1993-06-10
FI942644A0 (fi) 1994-06-03
MX9206979A (es) 1993-07-01

Similar Documents

Publication Publication Date Title
FI942644A0 (fi) Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin
FI962344A0 (fi) Järjestelmä ja menetelmä selluloosan sisäänruiskuttamiseksi
FI954330A (fi) EGIII -sellulaasin puhdistus ja molekyylikloonaus
FI944048A (fi) Mikrosomaalisen triglyseridia siirtävän proteiinin inhibiittoreita ja menetelmä
DE3480411D1 (en) Molecular cloning and expression in industrial microorganism species
SE9301347A0 (sv) Antiskummedel-komposition av emulsionstyp och sätt för skumbekämpning användande densamma
NO982672D0 (no) FremgangsmÕte for fremstilling av rekombinante proteiner i E. coli ved fermentering med h°y celletetthet
FI942419A0 (fi) Menetelmä alfa-interferonin valmistamiseksi ja puhdistamiseksi
AU2887897A (en) Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
DE59309088D1 (de) Herstellung von heterodimerem pdgf-ab mit hilfe eines bicistronischen vektorsystems in säugerzellen
FI942784A0 (fi) Samanaikaisen analogisen ja digitaalisen siirron sovellutuksia
FI963254A (fi) Alifaattinen polyesterihartsi ja menetelmä sen valmistamiseksi
FI91446B (fi) Menetelmä ja laitteisto hierteen freeneksen määrittämiseksi
FI943837A (fi) Ksylanaasi B:n kloonaus ja ilmentäminen
FI921059A0 (fi) Menetelmä hyötyproteiinien erittämiseksi hiivoista
DE58908785D1 (de) Veresterte Glycidylether-Additionsprodukte und deren Verwendung.
FI95548B (fi) Menetelmä ja laitteisto massiivipuun taivuttamiseksi
FI945994A0 (fi) Rekombinoitu sellulaasi
FI955000A0 (fi) Menetelmä polypeptidin läsnäolon paljastamiseksi ja sen muuttamiseksi
FI940403A0 (fi) Elliptisiinijohdannainen ja menetelmä sen valmistamiseksi
FI944575A0 (fi) Dekoriinigragmentteja ja menetelmiä solunsäätelytekijöiden inhiboimiseksi
FI964148A (fi) Julkisivumateriaali ja menetelmä sen valmistamiseksi
DE59107525D1 (de) Peptidamidase und deren Verwendung
FI943866A (fi) Aspergillus-lajien eksopolygalaturonaasi-geenin kloonaus ja ilmentäminen
DE3484912D1 (de) Peptidderivate und deren verwendung als substrate zur quantitativen bestimmung von enzymen.