FI942644A - Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin - Google Patents

Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin Download PDF

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Publication number
FI942644A
FI942644A FI942644A FI942644A FI942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A
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FI
Finland
Prior art keywords
cellulase
yeast
procedure
humicola insolence
cloning proteins
Prior art date
Application number
FI942644A
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English (en)
Swedish (sv)
Other versions
FI942644A0 (fi
Inventor
Henrik Dalboege
Hans Peter Heldt-Hansen
Grethe Rasmussen
Original Assignee
Novo Nordisk As
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Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of FI942644A0 publication Critical patent/FI942644A0/fi
Publication of FI942644A publication Critical patent/FI942644A/fi

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627Preparations containing enzymes, e.g. protease or amylase containing lipase
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38654Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
FI942644A 1991-12-04 1994-06-03 Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin FI942644A (fi)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK9100379 1991-12-04
DK9100378 1991-12-04
PCT/DK1992/000360 WO1993011249A1 (en) 1991-12-04 1992-12-02 A method of cloning proteins in yeast and cellulase from humicola insolens

Publications (2)

Publication Number Publication Date
FI942644A0 FI942644A0 (fi) 1994-06-03
FI942644A true FI942644A (fi) 1994-06-03

Family

ID=26068344

Family Applications (1)

Application Number Title Priority Date Filing Date
FI942644A FI942644A (fi) 1991-12-04 1994-06-03 Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin

Country Status (7)

Country Link
EP (1) EP0618974A1 (fi)
JP (1) JPH08504560A (fi)
BR (1) BR9206866A (fi)
CA (1) CA2124939A1 (fi)
FI (1) FI942644A (fi)
MX (1) MX9206979A (fi)
WO (1) WO1993011249A1 (fi)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE389012T1 (de) * 1994-10-06 2008-03-15 Novozymes As Ein enzympräparat mit endoglucanase aktivität
CA2203398A1 (en) * 1994-10-26 1996-05-09 Thomas Sandal An enzyme with lipolytic activity
MX9706974A (es) 1995-03-17 1997-11-29 Novo Nordisk As Endoglucanasas novedosas.
US6410701B1 (en) * 1995-05-05 2002-06-25 Human Genome Sciences, Inc. Human neuropeptide receptor
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
WO1997013853A2 (en) * 1995-10-13 1997-04-17 Gist-Brocades B.V. Protein detection
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
AU1438397A (en) * 1996-01-29 1997-08-22 Novo Nordisk A/S Process for desizing cellulosic fabric
CA2239576A1 (en) * 1996-01-29 1997-08-07 Novo Nordisk A/S Process for removal or bleaching of soiling or stains from cellulosic fabric
WO1997043409A2 (en) * 1996-05-10 1997-11-20 Novo Nordisk A/S Method of providing novel dna sequences
WO1997044470A1 (en) 1996-05-21 1997-11-27 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
EP0909273B1 (en) 1996-07-05 2005-10-26 Novozymes A/S Alpha-amylase transcription factor
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
ATE386799T1 (de) * 1996-10-28 2008-03-15 Novozymes As Extrazelluläre expression von zellulose bindenden domänen (cbd) unter verwendung von bacillus
ATE226974T1 (de) 1996-12-09 2002-11-15 Novozymes As Verringerung von phosphorhaltigen substanzen in speiseölen mit hohem anteil an nicht- hydratisierbarem phosphor unter verwendung einer phospholipase, eine phospholipase aus fädenförmigen pilz, welche eine phospholipase a und/oder b-aktivität aufweist
PL341760A1 (en) * 1997-12-22 2001-05-07 Dsm Nv Expression cloning in mildew fungi
CN1560609A (zh) * 1998-03-06 2005-01-05 ŵ����÷�����޹�˾ 荧光偏振筛选法
EP1086211B1 (en) 1998-06-10 2011-10-12 Novozymes A/S Novel mannanases
US6723504B1 (en) 1998-10-28 2004-04-20 Novozymes A/S Method for generating a gene library
WO2000024882A1 (en) * 1998-10-28 2000-05-04 Novozymes A/S Method for generating a gene library
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
EP1059351A1 (en) * 1999-06-11 2000-12-13 The Procter & Gamble Company Non-aqueous liquid detergent compositions comprising a borate-releasing compound and a mannanase
US7122330B2 (en) 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
CN102174551A (zh) * 2000-04-13 2011-09-07 戴亚蒂克国际(美国)公司 丝状真菌中dna表达文库的高通量筛选
AU2002339115B2 (en) 2001-05-18 2007-03-15 Dupont Nutrition Biosciences Aps Method of preparing a dough with an enzyme
AU2003206684A1 (en) * 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
DK2301958T3 (da) 2004-01-30 2014-06-16 Novozymes Inc Polypeptider med cellulolytisk forøgende aktivitet og polynukleotider, der koder for disse
CN101818177A (zh) 2004-07-16 2010-09-01 丹尼斯科公司 脂肪分解酶及其在食品工业中的应用
US8143482B2 (en) 2006-03-20 2012-03-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2102366A4 (en) 2006-12-10 2010-01-27 Dyadic International Inc EXPRESSION AND HIGH-DROP SCREENING OF COMPLEX EXPRESSED DNA LIBRARIES IN FADENED MUSHROOMS
EP2197893B1 (en) 2007-09-07 2013-07-24 Dyadic International, Inc. Novel fungal enzymes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
EP0406314B1 (en) * 1988-03-24 1993-12-01 Novo Nordisk A/S A cellulase preparation
DE69107455T3 (de) * 1990-05-09 2004-09-23 Novozymes A/S Eine ein endoglucanase enzym enthaltende zellulasezubereitung.
JPH05508547A (ja) * 1990-07-13 1993-12-02 トランスカリオティック セラピーズ,インコーポレイテッド ライブラリースクリーニング法

Also Published As

Publication number Publication date
FI942644A0 (fi) 1994-06-03
WO1993011249A1 (en) 1993-06-10
MX9206979A (es) 1993-07-01
JPH08504560A (ja) 1996-05-21
EP0618974A1 (en) 1994-10-12
BR9206866A (pt) 1995-11-21
CA2124939A1 (en) 1993-06-10

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