CA2124939A1 - A method of cloning proteins in yeast and cellulase from humicola insolens - Google Patents
A method of cloning proteins in yeast and cellulase from humicola insolensInfo
- Publication number
- CA2124939A1 CA2124939A1 CA002124939A CA2124939A CA2124939A1 CA 2124939 A1 CA2124939 A1 CA 2124939A1 CA 002124939 A CA002124939 A CA 002124939A CA 2124939 A CA2124939 A CA 2124939A CA 2124939 A1 CA2124939 A1 CA 2124939A1
- Authority
- CA
- Canada
- Prior art keywords
- enzyme
- protein
- interest
- seq
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
WOPCT/DK91/00378 | 1991-12-04 | ||
WOPCT/DK91/00379 | 1991-12-04 | ||
DK9100378 | 1991-12-04 | ||
DK9100379 | 1991-12-04 | ||
PCT/DK1992/000360 WO1993011249A1 (en) | 1991-12-04 | 1992-12-02 | A method of cloning proteins in yeast and cellulase from humicola insolens |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2124939A1 true CA2124939A1 (en) | 1993-06-10 |
Family
ID=26068344
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002124939A Abandoned CA2124939A1 (en) | 1991-12-04 | 1992-12-02 | A method of cloning proteins in yeast and cellulase from humicola insolens |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0618974A1 (fi) |
JP (1) | JPH08504560A (fi) |
BR (1) | BR9206866A (fi) |
CA (1) | CA2124939A1 (fi) |
FI (1) | FI942644A0 (fi) |
MX (1) | MX9206979A (fi) |
WO (1) | WO1993011249A1 (fi) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE389012T1 (de) * | 1994-10-06 | 2008-03-15 | Novozymes As | Ein enzympräparat mit endoglucanase aktivität |
EP0785994A1 (en) * | 1994-10-26 | 1997-07-30 | Novo Nordisk A/S | An enzyme with lipolytic activity |
AU715423B2 (en) | 1995-03-17 | 2000-02-03 | Novozymes A/S | Novel endoglucanases |
US6410701B1 (en) * | 1995-05-05 | 2002-06-25 | Human Genome Sciences, Inc. | Human neuropeptide receptor |
US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
AU7294396A (en) * | 1995-10-13 | 1997-04-30 | Gist-Brocades B.V. | Protein detection |
US6723549B2 (en) | 1995-10-17 | 2004-04-20 | Ab Enzymes Oy | Cellulases, the genes encoding them and uses thereof |
US6184019B1 (en) | 1995-10-17 | 2001-02-06 | Röhm Enzyme Finland OY | Cellulases, the genes encoding them and uses thereof |
AR005601A1 (es) * | 1996-01-29 | 1999-06-23 | Novozymes As | Proceso para la remocion o blanqueo de suciedad o manchas presentes en telas celulosicas y proceso para el lavado de telas celulosicas manchadas o sucias y composicion detergente |
CN1209838A (zh) * | 1996-01-29 | 1999-03-03 | 诺沃挪第克公司 | 纤维素织物的脱浆方法 |
AU3025597A (en) | 1996-05-10 | 1997-12-05 | Novo Nordisk A/S | Method of providing novel dna sequences |
WO1997044470A1 (en) | 1996-05-21 | 1997-11-27 | Novo Nordisk A/S | Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast |
AU3336597A (en) | 1996-07-05 | 1998-02-02 | Novo Nordisk A/S | A transcription factor |
US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
WO1998018905A1 (en) * | 1996-10-28 | 1998-05-07 | Novo Nordisk A/S | Extracellular expression of cellulose binding domains (cbd) using bacillus |
DE69716711T3 (de) | 1996-12-09 | 2010-06-10 | Novozymes A/S | Verringerung von phosphorhaltigen Substanzen in Speiseölen mit hohem Anteil an nicht-hydratisierbarem Phosphor unter Verwendung einer Phospholipase, eine Phospholipase aus fädenförmigen Pilz, welche eine Phospholipase A und/oder B-Aktivität aufweist |
DE69838106T2 (de) * | 1997-12-22 | 2008-04-03 | Dsm Ip Assets B.V. | Expressionsklonierung in filamentösen pilzen |
CN1560609A (zh) * | 1998-03-06 | 2005-01-05 | ŵ����÷������˾ | 荧光偏振筛选法 |
BRPI9911086B1 (pt) | 1998-06-10 | 2016-08-02 | Novozymes As | composição de limpeza, processo para tratar tecidos a máquina, e uso de uma mananase |
US6723504B1 (en) | 1998-10-28 | 2004-04-20 | Novozymes A/S | Method for generating a gene library |
CA2343878A1 (en) * | 1998-10-28 | 2000-05-04 | Novozymes A/S | Method for generating a gene library |
US7220542B2 (en) | 2000-07-17 | 2007-05-22 | Van Den Brink Johannes Maarten | Expression cloning in filamentous fungi |
EP1059351A1 (en) * | 1999-06-11 | 2000-12-13 | The Procter & Gamble Company | Non-aqueous liquid detergent compositions comprising a borate-releasing compound and a mannanase |
KR20020026456A (ko) | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 dna 라이브러리의 고산출량 스크리닝 |
DE60138947D1 (de) * | 2000-04-13 | 2009-07-23 | Dyadic Internat Usa Inc | Durchmusterung von bibliotheken exprimierter dna in filamentösen pilzen mit hoher durchsatzrate |
JP4309137B2 (ja) | 2001-05-18 | 2009-08-05 | ダニスコ エイ/エス | 酵素を使用した練り粉の調製方法 |
AU2003206684A1 (en) * | 2002-02-20 | 2003-09-09 | Novozymes A/S | Plant polypeptide production |
ES2469840T3 (es) * | 2004-01-30 | 2014-06-20 | Novozymes Inc. | Polip�ptidos con actividad de mejora celulol�tica y polinucle�tidos que los codifican |
AU2005263954B2 (en) | 2004-07-16 | 2011-04-07 | Dupont Nutrition Biosciences Aps | Enzymatic oil-degumming method |
EP1999257B1 (en) | 2006-03-20 | 2011-06-22 | Novozymes, Inc. | Polypeptides having endoglucanase activity and polynucleotides encoding same |
EP2505651A3 (en) | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Isolated fungus with reduced protease activity |
WO2009033071A2 (en) | 2007-09-07 | 2009-03-12 | Dyadic International, Inc. | Novel fungal enzymes |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK187280A (da) * | 1980-04-30 | 1981-10-31 | Novo Industri As | Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode |
EP0406314B1 (en) * | 1988-03-24 | 1993-12-01 | Novo Nordisk A/S | A cellulase preparation |
WO1991017243A1 (en) * | 1990-05-09 | 1991-11-14 | Novo Nordisk A/S | A cellulase preparation comprising an endoglucanase enzyme |
MY106504A (en) * | 1990-07-13 | 1995-06-30 | Transkaryotic Therapies Inc | Library screening method. |
-
1991
- 1991-12-04 MX MX9206979A patent/MX9206979A/es unknown
-
1992
- 1992-12-02 BR BR9206866A patent/BR9206866A/pt not_active Application Discontinuation
- 1992-12-02 JP JP5509731A patent/JPH08504560A/ja active Pending
- 1992-12-02 WO PCT/DK1992/000360 patent/WO1993011249A1/en not_active Application Discontinuation
- 1992-12-02 CA CA002124939A patent/CA2124939A1/en not_active Abandoned
- 1992-12-02 EP EP93900092A patent/EP0618974A1/en not_active Withdrawn
-
1994
- 1994-06-03 FI FI942644A patent/FI942644A0/fi unknown
Also Published As
Publication number | Publication date |
---|---|
WO1993011249A1 (en) | 1993-06-10 |
FI942644A (fi) | 1994-06-03 |
BR9206866A (pt) | 1995-11-21 |
EP0618974A1 (en) | 1994-10-12 |
JPH08504560A (ja) | 1996-05-21 |
MX9206979A (es) | 1993-07-01 |
FI942644A0 (fi) | 1994-06-03 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued | ||
FZDE | Discontinued |
Effective date: 20011203 |