CA2124939A1 - Methode pour le clonage de proteines dans une levure, et cellulase d'humicola insolens - Google Patents

Methode pour le clonage de proteines dans une levure, et cellulase d'humicola insolens

Info

Publication number
CA2124939A1
CA2124939A1 CA002124939A CA2124939A CA2124939A1 CA 2124939 A1 CA2124939 A1 CA 2124939A1 CA 002124939 A CA002124939 A CA 002124939A CA 2124939 A CA2124939 A CA 2124939A CA 2124939 A1 CA2124939 A1 CA 2124939A1
Authority
CA
Canada
Prior art keywords
enzyme
protein
interest
seq
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002124939A
Other languages
English (en)
Inventor
Henrik Dalboge
Hans P. Heldt-Hansen
Grethe Rasmussen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2124939A1 publication Critical patent/CA2124939A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002124939A 1991-12-04 1992-12-02 Methode pour le clonage de proteines dans une levure, et cellulase d'humicola insolens Abandoned CA2124939A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DK9100379 1991-12-04
WOPCT/DK91/00378 1991-12-04
WOPCT/DK91/00379 1991-12-04
DK9100378 1991-12-04
PCT/DK1992/000360 WO1993011249A1 (fr) 1991-12-04 1992-12-02 PROCEDE DE CLONAGE DE PROTEINES DANS LA LEVURE ET CELLULASE PROVENANT DE $i(HUMICOLA INSOLENS)

Publications (1)

Publication Number Publication Date
CA2124939A1 true CA2124939A1 (fr) 1993-06-10

Family

ID=26068344

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002124939A Abandoned CA2124939A1 (fr) 1991-12-04 1992-12-02 Methode pour le clonage de proteines dans une levure, et cellulase d'humicola insolens

Country Status (7)

Country Link
EP (1) EP0618974A1 (fr)
JP (1) JPH08504560A (fr)
BR (1) BR9206866A (fr)
CA (1) CA2124939A1 (fr)
FI (1) FI942644A (fr)
MX (1) MX9206979A (fr)
WO (1) WO1993011249A1 (fr)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE389012T1 (de) * 1994-10-06 2008-03-15 Novozymes As Ein enzympräparat mit endoglucanase aktivität
CA2203398A1 (fr) * 1994-10-26 1996-05-09 Thomas Sandal Enzyme a activite lipolytique
MX9706974A (es) 1995-03-17 1997-11-29 Novo Nordisk As Endoglucanasas novedosas.
US6410701B1 (en) * 1995-05-05 2002-06-25 Human Genome Sciences, Inc. Human neuropeptide receptor
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
WO1997013853A2 (fr) * 1995-10-13 1997-04-17 Gist-Brocades B.V. Detection de proteines
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
AU1438397A (en) * 1996-01-29 1997-08-22 Novo Nordisk A/S Process for desizing cellulosic fabric
CA2239576A1 (fr) * 1996-01-29 1997-08-07 Novo Nordisk A/S Procede de suppression ou de blanchiment des salissures ou des taches sur un tissu cellulosique
WO1997043409A2 (fr) * 1996-05-10 1997-11-20 Novo Nordisk A/S Procede d'obtention de nouvelles sequences d'adn
WO1997044470A1 (fr) 1996-05-21 1997-11-27 Novo Nordisk A/S Nouveaux promoteurs de levures convenant au clonage d'expression dans la levure et expression heterologue de proteines dans la levure
EP0909273B1 (fr) 1996-07-05 2005-10-26 Novozymes A/S FACTEUR DE TRANSCRIPTION du alpha-amylase
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
ATE386799T1 (de) * 1996-10-28 2008-03-15 Novozymes As Extrazelluläre expression von zellulose bindenden domänen (cbd) unter verwendung von bacillus
ATE226974T1 (de) 1996-12-09 2002-11-15 Novozymes As Verringerung von phosphorhaltigen substanzen in speiseölen mit hohem anteil an nicht- hydratisierbarem phosphor unter verwendung einer phospholipase, eine phospholipase aus fädenförmigen pilz, welche eine phospholipase a und/oder b-aktivität aufweist
PL341760A1 (en) * 1997-12-22 2001-05-07 Dsm Nv Expression cloning in mildew fungi
CN1560609A (zh) * 1998-03-06 2005-01-05 ŵ����÷�����޹�˾ 荧光偏振筛选法
EP1086211B1 (fr) 1998-06-10 2011-10-12 Novozymes A/S Nouvelles mannanases
US6723504B1 (en) 1998-10-28 2004-04-20 Novozymes A/S Method for generating a gene library
WO2000024882A1 (fr) * 1998-10-28 2000-05-04 Novozymes A/S Procede de creation d'une banque de genes
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
EP1059351A1 (fr) * 1999-06-11 2000-12-13 The Procter & Gamble Company Compositions détergentes liquides non-aqueuses contenant un composé libérant du borate et mannanase
US7122330B2 (en) 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
CN102174551A (zh) * 2000-04-13 2011-09-07 戴亚蒂克国际（美国）公司 丝状真菌中dna表达文库的高通量筛选
AU2002339115B2 (en) 2001-05-18 2007-03-15 Dupont Nutrition Biosciences Aps Method of preparing a dough with an enzyme
AU2003206684A1 (en) * 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
DK2301958T3 (da) 2004-01-30 2014-06-16 Novozymes Inc Polypeptider med cellulolytisk forøgende aktivitet og polynukleotider, der koder for disse
CN101818177A (zh) 2004-07-16 2010-09-01 丹尼斯科公司 脂肪分解酶及其在食品工业中的应用
US8143482B2 (en) 2006-03-20 2012-03-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2102366A4 (fr) 2006-12-10 2010-01-27 Dyadic International Inc Expression et criblage a haut debit de bibliotheques d'adn exprime complexes dans les champignons filamenteux
EP2197893B1 (fr) 2007-09-07 2013-07-24 Dyadic International, Inc. Enzymes fongiques inédites

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
EP0406314B1 (fr) * 1988-03-24 1993-12-01 Novo Nordisk A/S Preparation de cellulase
DE69107455T3 (de) * 1990-05-09 2004-09-23 Novozymes A/S Eine ein endoglucanase enzym enthaltende zellulasezubereitung.
JPH05508547A (ja) * 1990-07-13 1993-12-02 トランスカリオティック　セラピーズ，インコーポレイテッド ライブラリースクリーニング法

Also Published As

Publication number Publication date
FI942644A0 (fi) 1994-06-03
FI942644A (fi) 1994-06-03
WO1993011249A1 (fr) 1993-06-10
MX9206979A (es) 1993-07-01
JPH08504560A (ja) 1996-05-21
EP0618974A1 (fr) 1994-10-12
BR9206866A (pt) 1995-11-21

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued
FZDE Discontinued

Effective date: 20011203