FI942644A - Procedure for cloning proteins in yeast and cellulase from Humicola insolence - Google Patents

Procedure for cloning proteins in yeast and cellulase from Humicola insolence Download PDF

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Publication number
FI942644A
FI942644A FI942644A FI942644A FI942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A
Authority
FI
Finland
Prior art keywords
cellulase
yeast
procedure
humicola insolence
cloning proteins
Prior art date
Application number
FI942644A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI942644A0 (en
Inventor
Henrik Dalboege
Hans Peter Heldt-Hansen
Grethe Rasmussen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of FI942644A publication Critical patent/FI942644A/en
Publication of FI942644A0 publication Critical patent/FI942644A0/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
FI942644A 1991-12-04 1994-06-03 Procedure for cloning proteins in yeast and cellulase from Humicola insolence FI942644A0 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK9100378 1991-12-04
DK9100379 1991-12-04
PCT/DK1992/000360 WO1993011249A1 (en) 1991-12-04 1992-12-02 A method of cloning proteins in yeast and cellulase from humicola insolens

Publications (2)

Publication Number Publication Date
FI942644A true FI942644A (en) 1994-06-03
FI942644A0 FI942644A0 (en) 1994-06-03

Family

ID=26068344

Family Applications (1)

Application Number Title Priority Date Filing Date
FI942644A FI942644A0 (en) 1991-12-04 1994-06-03 Procedure for cloning proteins in yeast and cellulase from Humicola insolence

Country Status (7)

Country Link
EP (1) EP0618974A1 (en)
JP (1) JPH08504560A (en)
BR (1) BR9206866A (en)
CA (1) CA2124939A1 (en)
FI (1) FI942644A0 (en)
MX (1) MX9206979A (en)
WO (1) WO1993011249A1 (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3604595A (en) * 1994-10-06 1996-05-02 Novo Nordisk A/S An enzyme and enzyme preparation with endoglucanase activity
CN1167503A (en) * 1994-10-26 1997-12-10 诺沃挪第克公司 An enzyme with lipolytic activity
CN1182451A (en) 1995-03-17 1998-05-20 诺沃挪第克公司 Novel endoglucanases
US6410701B1 (en) * 1995-05-05 2002-06-25 Human Genome Sciences, Inc. Human neuropeptide receptor
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
WO1997013853A2 (en) * 1995-10-13 1997-04-17 Gist-Brocades B.V. Protein detection
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
AR005601A1 (en) * 1996-01-29 1999-06-23 Novozymes As PROCESS FOR THE REMOVAL OR WHITENING OF DIRT OR STAINS PRESENT IN CELLULOSIC FABRICS AND PROCESS FOR THE WASHING OF STAINED OR DIRTY CELLULOSIC FABRICS AND DETERGENT COMPOSITION
WO1997028256A1 (en) * 1996-01-29 1997-08-07 Novo Nordisk A/S Process for desizing cellulosic fabric
DE69738254T2 (en) * 1996-05-10 2008-08-14 Novozymes A/S METHOD FOR PROVISION OF DNA SEQUENCES
EP0922109A1 (en) 1996-05-21 1999-06-16 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
ATE307828T1 (en) 1996-07-05 2005-11-15 Novozymes As ALPHA-AMYLASE TRANSCRIPTION FACTOR
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
AU4699797A (en) * 1996-10-28 1998-05-22 Novo Nordisk A/S Extracellular expression of cellulose binding domains (cbd) using bacillus
ES2183113T5 (en) 1996-12-09 2010-03-31 Novozymes A/S REDUCTION OF COMPONENTS WITH CONTENT IN PHOSPHOLIPIDS IN EDIBLE OILS CONTAINING A RAISED AMOUNT OF NON-HYDRAPHABLE PHOSPHORUS, WITH THE USE OF A PHOSPHOLIPASE, OF A PHOSPHOLIPASE OF A FILAMENTOUS FUNGUS THAT PRESENTS O / PHOSPHOLE ACTIVITY B.
ES2287989T3 (en) * 1997-12-22 2007-12-16 Dsm Ip Assets B.V. EXPRESSION CLONATION IN FILAMENTOUS FUNGI.
AU2612299A (en) * 1998-03-06 1999-09-20 Novo Nordisk A/S A fluorescence polarization screening method
WO1999064619A2 (en) 1998-06-10 1999-12-16 Novozymes A/S Novel mannanases
US6723504B1 (en) 1998-10-28 2004-04-20 Novozymes A/S Method for generating a gene library
JP2002528075A (en) * 1998-10-28 2002-09-03 ノボザイムス アクティーゼルスカブ Gene library generation method
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
EP1059351A1 (en) * 1999-06-11 2000-12-13 The Procter & Gamble Company Non-aqueous liquid detergent compositions comprising a borate-releasing compound and a mannanase
MXPA01012905A (en) * 2000-04-13 2002-11-22 Mark Aaron Emalfarb HIGHminus;THROUGHPUT SCREENING OF EXPRESSED DNA LIBRARIES IN FILAMENTOUS FUNGI.
KR20020026456A (en) 2000-04-13 2002-04-10 에말파브 마크 아론 High-throughput screening of expressed dna libraries in filamentous fungi
AU2002339115B2 (en) 2001-05-18 2007-03-15 Dupont Nutrition Biosciences Aps Method of preparing a dough with an enzyme
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
ES2391826T3 (en) 2004-01-30 2012-11-30 Novozymes, Inc. Polypeptides with cellulolytic enhancement activity and polynucleotides that encode them
KR101226156B1 (en) 2004-07-16 2013-01-24 듀폰 뉴트리션 바이오사이언시즈 에이피에스 Enzymatic oil-degumming method
ES2368608T3 (en) 2006-03-20 2011-11-18 Novozymes, Inc. POLYPEPTIDES WITH ENDOGLUCANASE AND POLYNUCLEOTIDE ACTIVITY CODIFYING THE POLYPEPTIDES.
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
CA2736661A1 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK187280A (en) * 1980-04-30 1981-10-31 Novo Industri As RUIT REDUCING AGENT FOR A COMPLETE LAUNDRY
DE68911131T2 (en) * 1988-03-24 1994-03-31 Novonordisk As CELLULOSE PREPARATION.
WO1991017243A1 (en) * 1990-05-09 1991-11-14 Novo Nordisk A/S A cellulase preparation comprising an endoglucanase enzyme
ATE130049T1 (en) * 1990-07-13 1995-11-15 Transkaryotic Therapies Inc METHOD FOR SEARCHING A LIBRARY.

Also Published As

Publication number Publication date
BR9206866A (en) 1995-11-21
CA2124939A1 (en) 1993-06-10
WO1993011249A1 (en) 1993-06-10
MX9206979A (en) 1993-07-01
JPH08504560A (en) 1996-05-21
EP0618974A1 (en) 1994-10-12
FI942644A0 (en) 1994-06-03

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