FI86557B - Foerfarande foer framstaellning av alfaamylas medelst en mikroorganism av arten bacillus subtilis. - Google Patents

Foerfarande foer framstaellning av alfaamylas medelst en mikroorganism av arten bacillus subtilis. Download PDF

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Publication number
FI86557B
FI86557B FI844845A FI844845A FI86557B FI 86557 B FI86557 B FI 86557B FI 844845 A FI844845 A FI 844845A FI 844845 A FI844845 A FI 844845A FI 86557 B FI86557 B FI 86557B
Authority
FI
Finland
Prior art keywords
bacillus subtilis
amylase
fermentation
microorganism
alpha
Prior art date
Application number
FI844845A
Other languages
English (en)
Finnish (fi)
Other versions
FI86557C (sv
FI844845L (fi
FI844845A0 (fi
Inventor
Arved Lompe
Original Assignee
Solvay Enzymes Gmbh & Co Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Solvay Enzymes Gmbh & Co Kg filed Critical Solvay Enzymes Gmbh & Co Kg
Publication of FI844845A0 publication Critical patent/FI844845A0/fi
Publication of FI844845L publication Critical patent/FI844845L/fi
Publication of FI86557B publication Critical patent/FI86557B/fi
Application granted granted Critical
Publication of FI86557C publication Critical patent/FI86557C/sv

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (5)

1. Förfarande för framställning av alfaamylas genom fermen-tering pä i och för sig känt sätt av mikroorganismer av arten Bacillus subtilis i ett närmedium med en för mikroorganismer av arten Bacillus subtilis i och för sig känd sammansättning och eventuellt avskiljning av alfaamylasen ur fermenterings-mediet, kännetecknat av, att man fermenterar Bacillus subtilis DSM 2704 eller dess naturliga eller synte-tiska mutanter som vid fermentering bildar minst 200000 U/ml, fördelaktigt minst 250000 U/ml alfaamylas.
2. Förfarande enligt patentkravet 1, kännetecknat av, att man fermenterar Bacillus subtilis DSM 2704 eller dess naturliga eller eyntetiska mutanter som vid fermentering bildar minst 200000 U/ml, fördelaktigt minst 250000 U/ml alfaamylas och högst 10 NU/100000 amylasenheter, fördelaktigt högst 8 NU/100000 amylasenheter proteas.
3. Förfarande enligt patentkravet 1 eller 2, kännetecknat av, att fermenteringen utförs vid pH 5,0...9,0, fördelaktigt pH 6,0...8,0.
4. Förfarande enligt nägot av patentkraven 1...3, kännetecknat av, att fermenteringen utförs vid 25 °C...50 °C, fördelaktigt 33 °C...45 °C.
5. Förfarande enligt nägot av patentkraven 1...4, kännetecknat av, att fermenteringstiden är 30...90 timmar, fördelaktigt 50...70 timmar.
FI844845A 1983-12-09 1984-12-07 Förfarande för framställning av alfaamylas medelst en mikroorganism av arten Bacillus subtilis FI86557C (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP83112408 1983-12-09
EP83112408A EP0145798B1 (de) 1983-12-09 1983-12-09 Bacillus subtilis DSM 2704 und Verfahren zur Herstellung von Alpha-Amylase

Publications (4)

Publication Number Publication Date
FI844845A0 FI844845A0 (fi) 1984-12-07
FI844845L FI844845L (fi) 1985-06-10
FI86557B true FI86557B (fi) 1992-05-29
FI86557C FI86557C (sv) 1992-09-10

Family

ID=8190867

Family Applications (1)

Application Number Title Priority Date Filing Date
FI844845A FI86557C (sv) 1983-12-09 1984-12-07 Förfarande för framställning av alfaamylas medelst en mikroorganism av arten Bacillus subtilis

Country Status (7)

Country Link
US (1) US4610964A (sv)
EP (1) EP0145798B1 (sv)
JP (1) JPS60153791A (sv)
AT (1) ATE26303T1 (sv)
DE (1) DE3370652D1 (sv)
DK (1) DK164176C (sv)
FI (1) FI86557C (sv)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
DE3790919T1 (de) * 1987-02-24 1989-03-23 Inst Mikrobiologii Virusologii Arzneimittel zur prophylaxe und behandlung von gastrointerfinalen erkrankungen des landwirtschaftsviehs
US7550281B2 (en) * 2000-10-10 2009-06-23 The Hong Kong Polytechnic University Method for production of alpha-amylase in recombinant Bacillus
US20030134396A1 (en) * 2001-12-19 2003-07-17 Shetty Jayarama K. Process for hydrolyzing starch without pH adjustment
US9775610B2 (en) 2013-04-09 2017-10-03 Covidien Lp Apparatus for endoscopic procedures
US9700318B2 (en) 2013-04-09 2017-07-11 Covidien Lp Apparatus for endoscopic procedures
CN104450572B (zh) * 2014-12-04 2017-05-24 湖南新鸿鹰生物工程有限公司 一株产中温α‑淀粉酶的枯草芽孢杆菌

Also Published As

Publication number Publication date
ATE26303T1 (de) 1987-04-15
FI86557C (sv) 1992-09-10
JPS60153791A (ja) 1985-08-13
EP0145798B1 (de) 1987-04-01
JPH0436676B2 (sv) 1992-06-16
US4610964A (en) 1986-09-09
DK585784A (da) 1985-06-10
FI844845L (fi) 1985-06-10
FI844845A0 (fi) 1984-12-07
DE3370652D1 (en) 1987-05-07
DK164176B (da) 1992-05-18
DK585784D0 (da) 1984-12-07
EP0145798A1 (de) 1985-06-26
DK164176C (da) 1992-10-12

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