FI79139B - Foerfarande foer klonande av en gen, som kodar foer ett vaermebestaendigt -amylas, in i escherichia coli och bacillus subtilis. - Google Patents

Foerfarande foer klonande av en gen, som kodar foer ett vaermebestaendigt -amylas, in i escherichia coli och bacillus subtilis. Download PDF

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Publication number
FI79139B
FI79139B FI820107A FI820107A FI79139B FI 79139 B FI79139 B FI 79139B FI 820107 A FI820107 A FI 820107A FI 820107 A FI820107 A FI 820107A FI 79139 B FI79139 B FI 79139B
Authority
FI
Finland
Prior art keywords
amylase
plasmid
dna
coli
gene
Prior art date
Application number
FI820107A
Other languages
English (en)
Finnish (fi)
Other versions
FI79139C (sv
FI820107L (fi
Inventor
Jonathan R Mielenz
Susan Mickel
Original Assignee
Cpc International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cpc International Inc filed Critical Cpc International Inc
Publication of FI820107L publication Critical patent/FI820107L/fi
Publication of FI79139B publication Critical patent/FI79139B/fi
Application granted granted Critical
Publication of FI79139C publication Critical patent/FI79139C/sv

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Dental Preparations (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Claims (8)

1. Chimär plasmid med en gen, som kodar för termos-tabilt a-amylas, kännetecknad därav, att den 5 innehäller en DNA-sekvens, som innehäller α-amylasgenen frän plasmid-DNA, som erhällits ur en Bacillus stearother-mophilus-stam och DNA pä en lämplig E. coli- eller B. sub-tilis-vektor.
2. Chimär plasmid enligt patentkravet 1, k ä n - 10 netecknad därav, att DNA-sekvensen som innehäller a-amylasgener erhälles ur B. stearothermophilusstammen ATCC 31 195, 31 196, 31 197, 31 198, 31 199 eller 31 783.
3. Förfarande för konstruktion av en chimär plasmid enligt patentkravet 1 med en gen, som kodar termosta- 15 bilt α-amylas, där man a) spjälker ett i naturen förekommande DNA av mik-roorganismursprung, vilken innehäller en gen som kodar för termostabilt α-amylas, med restriktionsendonukleas Hind III för erhällande av en lineär DNA-sekvens innehällande 20 a-amylasgenen; b) spjälker en lämplig E. coli- eller B-subtilis-vektor med restriktionsendonukleas Hind III för erhällande av en annan lineär DNA-sekvens, och c) sammanbinder de i stegen a) och b) erhällna li- 25 neära DNA-sekvensserna med T4-DNA-ligas, känne- t e c k n a t därav, att det i naturen förekommande DNA:t av mikroorganismursprung är plasmid-DNA som erhällits ur en Bacillus stearothermophilus-stam.
4. Förfarande enligt patentkravet 3, känne- 30 tecknat därav, att vektorn är en plasmid, som är E. coli-plasmiden pBR322, pBR325 eller pWL625 eller B. sub-tilis plasmiden pC194.
5. Förfarande enligt patentkravet 3 eller 4, kännetecknat därav, att det i naturen förekom- 11
FI820107A 1981-01-15 1982-01-13 Förfarande för klonande av en gen, som kodar för ett värmebeständigt a-amylas, in i Escherichia coli och Bacillus subtilis FI79139C (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22528781A 1981-01-15 1981-01-15
US22528781 1981-01-15

Publications (3)

Publication Number Publication Date
FI820107L FI820107L (fi) 1982-07-16
FI79139B true FI79139B (fi) 1989-07-31
FI79139C FI79139C (sv) 1989-11-10

Family

ID=22844306

Family Applications (1)

Application Number Title Priority Date Filing Date
FI820107A FI79139C (sv) 1981-01-15 1982-01-13 Förfarande för klonande av en gen, som kodar för ett värmebeständigt a-amylas, in i Escherichia coli och Bacillus subtilis

Country Status (16)

Country Link
EP (1) EP0057976B1 (sv)
JP (1) JPS57139097A (sv)
AT (1) ATE17373T1 (sv)
CA (1) CA1170202A (sv)
DD (1) DD208988A5 (sv)
DE (1) DE3268340D1 (sv)
DK (1) DK157879C (sv)
ES (2) ES8305041A1 (sv)
FI (1) FI79139C (sv)
HU (1) HU193516B (sv)
IE (1) IE52434B1 (sv)
IL (1) IL64741A (sv)
MX (1) MX7066E (sv)
SU (1) SU1200853A3 (sv)
YU (2) YU9682A (sv)
ZA (1) ZA82133B (sv)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4886754A (en) * 1980-01-07 1989-12-12 The University Of Rochester Recombinant bateriophage for heterologous cloning of bacillus microorganisms and method for its production
FR2533583A1 (fr) * 1982-09-24 1984-03-30 Centre Nat Rech Scient Nouvel adn recombinant utile dans la preparation d'a-amylase
NO170594C (no) * 1982-11-01 1992-11-04 Solvay Enzymes Inc Fremgangsmaate for heterolog kloning av gen i bacillus mikroorganisme
US4559300A (en) * 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
JPS59196092A (ja) * 1983-04-25 1984-11-07 Sanraku Inc 組換えプラスミドによる耐熱性α−アミラ−ゼの新規製造法
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
AU587960B2 (en) * 1983-06-24 1989-09-07 Genentech Inc. Procaryotic carbonyl hydrolases
US4578352A (en) * 1983-07-13 1986-03-25 Cpc International Inc. Novel thermostable, aciduric alpha-amylase and method for its production
GB8333797D0 (en) * 1983-12-19 1984-01-25 Searle & Co Starch utilization
US5032510A (en) * 1984-09-26 1991-07-16 Eli Lilly And Company Method for expression and secretion in bacillus
AU5668486A (en) * 1985-03-29 1986-10-23 Biotechnica International Inc. Secretion vector
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
US5278059A (en) * 1985-12-04 1994-01-11 Kabushiki Kaisha Hayashibara Seibutsu Kagaki Kenkyujo Polypeptide possessing cyclomaltodextrin glucanotransferase activity
IN165610B (sv) * 1986-12-22 1989-11-25 Enzyme Bio Systems Ltd
US4977089A (en) * 1987-01-30 1990-12-11 Eli Lilly And Company Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
DE69032360T2 (de) * 1989-06-29 1998-12-03 Genencor Int Mutierte mikrobielle alpha-Amylasen mit erhöhter thermischer, saurer und/oder alkylischer Stabilität
NL8902128A (nl) * 1989-08-23 1991-03-18 Avebe Coop Verkoop Prod Vertakkingsenzym en gebruik daarvan.
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ183818A (en) * 1976-04-19 1980-05-08 Cpc International Inc Heat -and acid-stable alpha-amylase and process for converting starch to a starch hydrolysate
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes

Also Published As

Publication number Publication date
ZA82133B (en) 1983-02-23
IE820032L (en) 1982-07-15
CA1170202A (en) 1984-07-03
IL64741A0 (en) 1982-03-31
DK13182A (da) 1982-07-16
DE3268340D1 (en) 1986-02-20
SU1200853A3 (ru) 1985-12-23
YU9682A (en) 1985-06-30
IL64741A (en) 1985-07-31
ES8405070A1 (es) 1984-05-16
DD208988A5 (de) 1984-04-18
EP0057976A2 (en) 1982-08-18
YU176384A (en) 1987-02-28
DK157879C (da) 1990-07-30
MX7066E (es) 1987-04-20
ES508694A0 (es) 1983-03-16
IE52434B1 (en) 1987-10-28
JPS57139097A (en) 1982-08-27
EP0057976B1 (en) 1986-01-08
ES518157A0 (es) 1984-05-16
DK157879B (da) 1990-02-26
ATE17373T1 (de) 1986-01-15
EP0057976A3 (en) 1982-09-01
HU193516B (en) 1987-10-28
FI79139C (sv) 1989-11-10
ES8305041A1 (es) 1983-03-16
FI820107L (fi) 1982-07-16

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Owner name: CPC INTERNATIONAL INC.