FI120835B - Nya esteras och deras användning - Google Patents

Nya esteras och deras användning Download PDF

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Publication number
FI120835B
FI120835B FI20075532A FI20075532A FI120835B FI 120835 B FI120835 B FI 120835B FI 20075532 A FI20075532 A FI 20075532A FI 20075532 A FI20075532 A FI 20075532A FI 120835 B FI120835 B FI 120835B
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FI
Finland
Prior art keywords
protein
activity
seq
polynucleotide
cutinase
Prior art date
Application number
FI20075532A
Other languages
English (en)
Finnish (fi)
Other versions
FI20075532A0 (sv
FI20075532A (sv
Inventor
Johanna Buchert
Marjaana Raettoe
Tiina Nakari-Setaelae
Pasi Halonen
Hanna Kontkanen
Ann Westerholm-Parvinen
Original Assignee
Valtion Teknillinen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valtion Teknillinen filed Critical Valtion Teknillinen
Publication of FI20075532A0 publication Critical patent/FI20075532A0/sv
Priority to FI20075532A priority Critical patent/FI120835B/sv
Priority to JP2010515544A priority patent/JP5496883B2/ja
Priority to CA 2692605 priority patent/CA2692605A1/en
Priority to EP08787696.7A priority patent/EP2171050B1/en
Priority to US12/668,313 priority patent/US8546115B2/en
Priority to AU2008274161A priority patent/AU2008274161B2/en
Priority to CN200880102955A priority patent/CN101784657A/zh
Priority to PCT/FI2008/050419 priority patent/WO2009007510A1/en
Priority to BRPI0814694A priority patent/BRPI0814694A2/pt
Publication of FI20075532A publication Critical patent/FI20075532A/sv
Priority to ZA201000436A priority patent/ZA201000436B/xx
Application granted granted Critical
Publication of FI120835B publication Critical patent/FI120835B/sv

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
    • D06M2101/16—Synthetic fibres, other than mineral fibres
    • D06M2101/30—Synthetic polymers consisting of macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • D06M2101/32—Polyesters

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Textile Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing Of Solid Wastes (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Claims (20)

1. Polyesterasprotein, som innehäller en aminosyrasekvens som har ätminstone 50-procents identitet med sekvens nr 2 eller 6, eller en variant 5 därav med polyesterasaktivitet.
2. Protein enligt patentkrav 1, vilket protein innehäller en aminosyrasekvens enligt sekvens nr 4, 7, 8 eller 9, eller en variant därav med polyesterasaktivitet.
3. Protein enligt patentkrav 1, vilket protein har atminstone 80-, 90-, 10 95- eller 98-procents identitet med sekvens nr 2, 4 eller 6.
4. Protein enligt patentkrav 1, vilket protein har minst kutinas- eller suberinasaktivitet eller bada.
5. Protein enligt patentkrav 4, vilket dessutom har lipasaktivitet.
6. Protein enligt patentkrav 1, vilket protein härstammar frän svam-15 pen Coprinus, företrädesvis Coprinus cinereus.
7. Protein enligt patentkrav 6, vilket protein härstammar frän svam-pen C. cinereus, och vilket innehäller en aminosyrasekvens som motsvarar sekvens nr 2, 4, 6, 7, 8 eller 9, eller en variant därav, med minst kutinas- eller suberinasaktivitet eller bäda.
8. Isolerad polynukleotid, som valts ur gruppen som bestär av: a) en polynukleotid som innehäller en nukleotidsekvens enligt sekvens nr 1, 3 eller 5, eller en nukleotidsekvens som kodar för ett protein enligt patentkrav 1, b) en sekvens som är komplementär till sekvensen i punkt a), och 25 c) en sekvens, som är degenererad i förhällande till den genetiska koden till en sekvens enligt punkt a) eller b).
9. Vektor som innehäller en polynukleotid enligt patentkrav 8.
10. Genetiskt modifierad mikroorganism, som transformerats med en vektor enligt patentkrav 9. 30
11. Förfarande för att framställa ett polyesterasprotein enligt patent krav 1, vilket förfarande omfattar steg där man transformerar en mikroorganism med en vektor som innehäller en polynukleotid enligt patentkrav 8, odlar den transformerade mikroorganismen under betingelser som tilläter expression av nämnda polynukleotid, och tar tillvara det expresserade proteinet. 35
12. Förfarande enligt patentkrav 11, där polynukleotiden erhälls ur svampen Coprinus cinereus, och expresseras i en värd, som väljs ur gruppen som bestär av: Trichoderma, Saccharomyces, Pichia, Aspergillus och bakteri-er, företrädesvis är värden T. reesei.
13. Enzympreparat som innehäller ett protein enligt patentkrav 1.
14. Förfarande för att hydrolysera kutin, suberin eller nägon annan 5 polyester, vilket förfarande innehäller steg där man behandlar kutin, suberin eller nägot annat material som innehäller polyester med ett protein enligt patentkrav 1 under betingelser som tilläter partiell eller total hydrolys av nämnda polyester.
15. Förfarande enligt patentkrav 14, vilket omfattar ett steg där man 10 behandlar rävaror för jordbruk eller livsmedel eller biprodukter som erhällits ur grönsaker, frukter, bär, eller säd med nämnda protein.
16. Förfarande enligt patentkrav 14, vilket omfattar ett steg där man behandlar trärävaror, massa- eller pappersprodukter, processavfall eller -vatten eller biprodukter med nämnda protein. 15
17. Förfarande enligt patentkrav 14, vilket omfattar ett steg där man modifierar syntetiska eller andra av mänskan framställda polyesterfiber eller -textiler med nämnda protein.
18. Förfarande enligt patentkrav 14, vilket omfattar ett steg där man med nämnda protein avlägsnar fläckar eller fett frän byke eller disk. 20
19. Förfarande enligt patentkrav 14, vilket omfattar ett steg där man depolymeriserar kutin eller suberin med nämnda protein.
20. Användning av ett protein enligt patentkrav 1, eller ett enzympreparat enligt patentkrav 13 inom livsmedelsindustrin, massa- eller pappers-industrin, textilindustrin, eller inom tvätt- eller diskmedelstillämpningar eller i 25 kemisk syntes.
FI20075532A 2007-07-10 2007-07-10 Nya esteras och deras användning FI120835B (sv)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FI20075532A FI120835B (sv) 2007-07-10 2007-07-10 Nya esteras och deras användning
US12/668,313 US8546115B2 (en) 2007-07-10 2008-07-08 Esterases and their use
CA 2692605 CA2692605A1 (en) 2007-07-10 2008-07-08 Novel esterases and their use
EP08787696.7A EP2171050B1 (en) 2007-07-10 2008-07-08 Novel esterases and their use
JP2010515544A JP5496883B2 (ja) 2007-07-10 2008-07-08 新規のエステラーゼおよびその使用
AU2008274161A AU2008274161B2 (en) 2007-07-10 2008-07-08 Novel esterases and their use
CN200880102955A CN101784657A (zh) 2007-07-10 2008-07-08 新型酯酶及其用途
PCT/FI2008/050419 WO2009007510A1 (en) 2007-07-10 2008-07-08 Novel esterases and their use
BRPI0814694A BRPI0814694A2 (pt) 2007-07-10 2008-07-08 novas esterases e seu uso
ZA201000436A ZA201000436B (en) 2007-07-10 2010-01-20 Novel esterases and their use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20075532A FI120835B (sv) 2007-07-10 2007-07-10 Nya esteras och deras användning
FI20075532 2007-07-10

Publications (3)

Publication Number Publication Date
FI20075532A0 FI20075532A0 (sv) 2007-07-10
FI20075532A FI20075532A (sv) 2009-01-11
FI120835B true FI120835B (sv) 2010-03-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
FI20075532A FI120835B (sv) 2007-07-10 2007-07-10 Nya esteras och deras användning

Country Status (10)

Country Link
US (1) US8546115B2 (sv)
EP (1) EP2171050B1 (sv)
JP (1) JP5496883B2 (sv)
CN (1) CN101784657A (sv)
AU (1) AU2008274161B2 (sv)
BR (1) BRPI0814694A2 (sv)
CA (1) CA2692605A1 (sv)
FI (1) FI120835B (sv)
WO (1) WO2009007510A1 (sv)
ZA (1) ZA201000436B (sv)

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Also Published As

Publication number Publication date
WO2009007510A1 (en) 2009-01-15
CN101784657A (zh) 2010-07-21
CA2692605A1 (en) 2009-01-15
FI20075532A0 (sv) 2007-07-10
FI20075532A (sv) 2009-01-11
US8546115B2 (en) 2013-10-01
BRPI0814694A2 (pt) 2016-07-19
AU2008274161A1 (en) 2009-01-15
JP5496883B2 (ja) 2014-05-21
US20100209985A1 (en) 2010-08-19
EP2171050A4 (en) 2010-09-01
JP2010532984A (ja) 2010-10-21
EP2171050B1 (en) 2015-03-18
EP2171050A1 (en) 2010-04-07
ZA201000436B (en) 2010-10-27
AU2008274161B2 (en) 2014-07-24

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