FI119552B - System och förfarande för manipulering av magnetiska partiklar i vätskeprov för utvinning av DNA eller RNA från provet - Google Patents
System och förfarande för manipulering av magnetiska partiklar i vätskeprov för utvinning av DNA eller RNA från provet Download PDFInfo
- Publication number
- FI119552B FI119552B FI20020923A FI20020923A FI119552B FI 119552 B FI119552 B FI 119552B FI 20020923 A FI20020923 A FI 20020923A FI 20020923 A FI20020923 A FI 20020923A FI 119552 B FI119552 B FI 119552B
- Authority
- FI
- Finland
- Prior art keywords
- sample tube
- magnet
- particles
- sample
- solution
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 38
- 239000006249 magnetic particle Substances 0.000 title description 9
- 239000007788 liquid Substances 0.000 title description 5
- 239000002245 particle Substances 0.000 claims description 83
- 150000007523 nucleic acids Chemical class 0.000 claims description 53
- 102000039446 nucleic acids Human genes 0.000 claims description 51
- 108020004707 nucleic acids Proteins 0.000 claims description 51
- 230000005291 magnetic effect Effects 0.000 claims description 28
- 230000005415 magnetization Effects 0.000 claims 2
- 239000000523 sample Substances 0.000 description 154
- 239000000243 solution Substances 0.000 description 66
- 238000000926 separation method Methods 0.000 description 15
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 12
- 238000010828 elution Methods 0.000 description 12
- 239000003929 acidic solution Substances 0.000 description 11
- 238000001816 cooling Methods 0.000 description 11
- 238000003556 assay Methods 0.000 description 10
- 230000005298 paramagnetic effect Effects 0.000 description 10
- 238000005406 washing Methods 0.000 description 9
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 238000002203 pretreatment Methods 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 4
- 238000000354 decomposition reaction Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000003480 eluent Substances 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000007826 nucleic acid assay Methods 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 241001272996 Polyphylla fullo Species 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000005294 ferromagnetic effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910001172 neodymium magnet Inorganic materials 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000037452 priming Effects 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 238000001216 nucleic acid method Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/103—General features of the devices using disposable tips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (11)
1. System för behandling av sädana magnetiskt reagerande partiklar vid vilka har fästs nukleinsyramolekyler och som är belägna i en lösning närvarande i ätmin- 5 stone ett provrör, kännetecknat av att nämnda system innefattar: en hällare (170) för provröret (120) innefattande ätminstone en öppning (172) av-sedd för provröret, som är gjort för inpassning av nämnda provrör (120); 10 ätminstone en första magnet (166); en flyttanordning för magneten, som är utförd att flytta nämnda första magnet (166) selektivt i förhällande tili nämnda provrör (120) mellan ett första läge, i vilket den är avsedd att dra tili sig magnetiskt reagerande partiklar (190) mot nämnda provrörs 15 (120) inre vägg, och ett andra läge i förhällande tili nämnda provrör (120), i vilket avsikten är att möjliggöra suspendering av nämnda magnetiskt reagerande partiklar (190) i nämnda lösning; och en andra magnet (178), som är utförd att rikta ett magnetfält mot nämnda magne-20 tiskt reagerande partiklar (190), dä nämnda första magnet (166) positionerats i nämnda andra läge, varvid avsikten är att avlägsna magnetiseringen som nämnda :*·*: första magnet (166) har alstrat i nämnda magnetiskt reagerande partiklar (190). • · • * * • ·· • ·
2. System enligt patentkrav 1, kännetecknat av att nämnda andra magnet (178) .···, 25 innefattar en växelströmelektromagnet (180) och nämnda magnetfält innefattar ett • · växelströmmagnetfält.
• ♦ ♦ • · • · · • · * · · · ’ 3. System enligt patentkrav 1, kännetecknat av att nämnda andra magnet (178) är väsentligen stationär i förhällande tili nämnda provrör (120). I'*·· 30 • · ·
4. System enligt patentkrav 1, kännetecknat av att nämnda första (166) och andra magnet (178) är placerade väsentligen pä motsatta sidor av nämnda provrör (120). • · • · V.: 35
5. System enligt patentkrav 1, kännetecknat av att nämnda andra magnet (178) • · · är placerad under undersidan av nämnda provrörs öppning (172). 119552 22
6. Förfarande för att behandla sädana magnetiskt reagerande partiklar, vid vilka har fästs nukleinsyramolekyler och som är belägna i en lösning, som är ätminstone i ett provrör, kännetecknat av att nämnda förfarande innefattar att: 5 nämnda provrör (120) placeras i den öppning (172) av provrörets hällare (170), i vilken provröret (120) inpassas; den första magneten (166) flyttas selektivt i förhällande tili nämnda provrör (120) i ett första läge, i vilket den är avsedd att dra tili sig magnetiskt reagerande partiklar 10 (190) mot nämnda provrörs (120) inre vägg, och i ett andra läge i förhällande tili nämnda provrör (120), varvid avsikten är att möjliggöra en suspendering av nämnda magnetiskt reagerande partiklar (190) i nämnda lösning; och ett magnetfält rikas mot nämnda magnetiskt reagerande partiklar (190), dä nämnda 15 första magnet (166) positionerats i nämnda andra läge, varvid avsikten är att väsent-ligen avlägsna magnetiseringen som nämnda första magnet (166) har alstrat i nämnda magnetiskt reagerande partiklar (190).
7. Förfarande enligt patentkrav 6, kännetecknat av att nämnda magnetfält in-20 nefattar ett växelströmmagnetfält.
:***; 8. Förfarande enligt patentkrav 6, kännetecknat av att det innefattar ätminsto- :1·.· ne det ena av förfaranden, vid vilka nämnda lösning i nämnda provrör (120) utsätts • · för termisk energi och varvid termisk energi avlägsnas frän lösningen i nämnda .···. 25 provrör (120). • · ··· • · • · ·
9. Förfarande enligt patentkrav 6, kännetecknat av att nämnda magnetflytt- • « *···' ningsskede flyttar nämnda magnet (166) mellan nämnda första och andra ställe i en första riktning, som är väsentligt parallell med nämnda provrörs (120) längdaxel. I1’·· 30 ··«
10. Förfarande enligt patentkrav 6, kännetecknat av att nämnda magnetfält i nämnda inriktningsskede positioneras manuellt nedifrän frän undersidan av öpp- • · ..... ningen (172) i nämnda rör (120). • · · • · » t · 1 • · φ · · • · • · »»·
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/858,889 US6672458B2 (en) | 2000-05-19 | 2001-05-17 | System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample |
| US85888901 | 2001-05-17 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI20020923A0 FI20020923A0 (sv) | 2002-05-16 |
| FI20020923L FI20020923L (sv) | 2002-11-18 |
| FI119552B true FI119552B (sv) | 2008-12-31 |
Family
ID=25329438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI20020923A FI119552B (sv) | 2001-05-17 | 2002-05-16 | System och förfarande för manipulering av magnetiska partiklar i vätskeprov för utvinning av DNA eller RNA från provet |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6672458B2 (sv) |
| EP (1) | EP1260583A1 (sv) |
| JP (1) | JP3676754B2 (sv) |
| AU (1) | AU785010B2 (sv) |
| BR (1) | BR0201515B1 (sv) |
| CA (1) | CA2380919C (sv) |
| FI (1) | FI119552B (sv) |
| NO (1) | NO330113B1 (sv) |
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- 2002-05-16 FI FI20020923A patent/FI119552B/sv not_active IP Right Cessation
- 2002-10-11 US US10/269,903 patent/US20030038071A1/en not_active Abandoned
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| CA2380919A1 (en) | 2002-11-17 |
| JP2003093918A (ja) | 2003-04-02 |
| AU4059102A (en) | 2002-11-21 |
| JP3676754B2 (ja) | 2005-07-27 |
| FI20020923A0 (sv) | 2002-05-16 |
| US6672458B2 (en) | 2004-01-06 |
| US20020014443A1 (en) | 2002-02-07 |
| NO20022351D0 (no) | 2002-05-16 |
| FI20020923L (sv) | 2002-11-18 |
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