FI111011B - Fast tillväxtsunderlag för mikroorganismer, dess framställning och användning - Google Patents
Fast tillväxtsunderlag för mikroorganismer, dess framställning och användning Download PDFInfo
- Publication number
- FI111011B FI111011B FI970163A FI970163A FI111011B FI 111011 B FI111011 B FI 111011B FI 970163 A FI970163 A FI 970163A FI 970163 A FI970163 A FI 970163A FI 111011 B FI111011 B FI 111011B
- Authority
- FI
- Finland
- Prior art keywords
- hydrogel
- medium
- cellulose ethers
- cellulose
- gel
- Prior art date
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- 229920002223 polystyrene Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 229920001290 polyvinyl ester Polymers 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000012966 redox initiator Substances 0.000 description 1
- 239000005871 repellent Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- SKMHHHHLLBKNKR-UHFFFAOYSA-M sodium;prop-2-enamide;prop-2-enoate Chemical compound [Na+].NC(=O)C=C.[O-]C(=O)C=C SKMHHHHLLBKNKR-UHFFFAOYSA-M 0.000 description 1
- 239000002594 sorbent Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- RRHXZLALVWBDKH-UHFFFAOYSA-M trimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]azanium;chloride Chemical compound [Cl-].CC(=C)C(=O)OCC[N+](C)(C)C RRHXZLALVWBDKH-UHFFFAOYSA-M 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229920001567 vinyl ester resin Chemical class 0.000 description 1
- 239000004552 water soluble powder Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- 229910052725 zinc Inorganic materials 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S524/00—Synthetic resins or natural rubbers -- part of the class 520 series
- Y10S524/916—Hydrogel compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Claims (18)
1. Torkat hydrogelodlingsmedium för mikrober, känneteck-nat därav, att det omfattar en hydrogel baserad pä cellu-losaetrar med kovalenta tvärbindningar, varvid hydrogel- 5 odlingsmediet kan fästas direkt pä ett för vatten ogenomträng1igt underlag utan bärare- eller adhesions-skikt.
2. Hydrogelodlingsmedium enligt patentkravet 1, kännetecknat därav, att den gelbildande beständsdelen inne- 10 halier bäde hydrofila och hydrofoba cellulosaetrar.
3. Hydrogelodlingsmedium enligt patentkravet 1 eller 2, kannetecknat därav, att väte i cellulosans OH-grupper i cellulosaetrar har substituerats med alkyl-, som till exempel metyl-, etyl- eller propylgrupper, hydroxialkyl-, 15 som till exempel hydroxietyl- eller hydroxipropylgrupper, bensyl- eller hydroxibensylgrupper eller med karboximety-lens alkalimetallsalter, varvid i var och en glukosenhet hos cellulosan kan vara 0-3 OH-grupper, som substituerats med ovannämnda grupper.
4. Hydrogelodlingsmedium enligt nägot av de föregäende patentkraven, kännetecknat därav, att cellulosaeterns mo-lekylvikt är 500 - 1 000 000, företrädesvis 1 000 - 500 000. «
5 NjN'-bismetylenakrylamid, till den erhällna tvärbundna hydrogelen tillsätts näring-sämnen samt eventuellt andra behövliga komponenter, och det erhällna hydrogelodlingsmediet utbreds pä ett för vatten ogenomträngligt underlag och torkas, varvid od-10 lingsmediet fastnar direkt pä underlaget utan bärare- eller adhesionsskikt.
5. Hydrogelodlingsmedium enligt nägot av de föregäende 25 patentkraven, kännetecknat därav, att cellulosaeterns viskositet är 100 - 100 000 mPas, heist 1 000 - 10 000 mPas (2 % lösning, 20 °C).
,! 6. Hydrogelodlingsmedium enligt nägot av de föregäende patentkraven, kännetecknat därav, att porstorleken i hyd-30 rogelen, som baserar sig pä tvärbundna cellulosaetrar, är 0,1 - 1000 nm, företrädesvis 1,0 - 500 nm.
7. Hydrogelodlingsmedium enligt nägot av de föregäende 111011 patentkraven, kännetecknat därav, att det utöver den pä tvärbundna cellulosaetrar baserade hydrogelen omfattar näringsämnen samt eventuellt andra behövliga komponenter, ' säsom andra gelbildande föreningar, pH-regleringsmedel, 5 detergenter, substrat, andra reagens som hör tili bioke-' miska detektionsreaktioner, selektiva inhibitorer, anti- biotika och färgmedel.
8. Hydrogelodlingsmedium enligt nägot av de föregäende patentkraven, kännetecknat därav, att det har torkats pä 10 ett för vatten ogenomträngligt underlag tili en fuktighet av 0,01 - 20 vikt-%.
9. Hydrogelodlingsmedium enligt patentkravet 1, kännetecknat därav, att tili gelen kan tillsättas cellulosaetrar, kopolymerer polymeriserade av vinylmonomerer, 15 som akrolein, akrylamid, 2-akrylamido-2-metylpropansul-fonsyra, 2-akrylamido-2-metylpropansulfonsyrans salter, akrylsyra, akrylsyrans salter, akrylonitril, metakryla-mid, metakrylamidopropyltrimetylammoniumklorid, metakryl-syra, metakrylsyrans salter, 2-metakroyloxietansulfonsy-20 ra, 2-metakroyloxietansulfonsyrans salter, 2-metakrylo- xietyltrimetylammoniumklorid, 3-metakryloxi-2-hydroxipro-pyltrimetylammoniumklorid, metakroylklorid, Ν,Ν'-metylen-bisakrylamid eller blandningar därav, agar-agar, albu-miner, alginat, gelatin, guargum, karragener, xantangum-25 mi, polypeptider och andra modifierade polysackarider.
10. Förfarande för framställning av ett torkat hydroge-lodlingsunderlag för mikrober, kännetecknat därav, att cellulosaetrar tvärbinds tili varandra för bildning av hydrogel t · j · * 30 a) genom att omsätta cellulosaetrarnas OH-grupper med till exempel divinylsulfon, formaldehyd, glyoxal, urea eller monometylol- och dimetylolderivat av melamin, ureaformaldehyd- och melaminformaldehydförpolymerer, di-metyloletylenurea, epiklorhydrin, diepoxider eller diiso- 111011 cyanat, eller b) genom att besträla eller c) genom att graftpolymerisera cellulosaetrarna med fri-radikalmekanism med vinylmonomerer, säsom akrylamid och
11. Förfarande enligt patentkravet 10, kännetecknat dä-rav, att tili gelen tillsätts utöver näringsämnen ocksä andra komponenter, säsom gelbildande föreningar, som ab- 15 sorberar vattenlösningar, pH-regleringsmedel, detergen-ter, substrat, andra reagens som hör tili biokemiska de-tektionsreaktioner, selektiva inhibitorer, antibiotika eller färgmedel, och det erhällna hydrogelodlingsmediet utbreds pä ett för vatten ogenomträngligt underlag, tor-20 kas vid 20 - 60 °C tili en fuktighet av 0,01 - 20 vikt-% och klipps tili testremsor.
12. Förfarande enlig patentkravet 10 eller 11, kännetecknat därav, att när man tvärbinder cellulosaetrar enligt alternativet a) av patentkravet 11, är den teoretiska 25 tvärbindingstätheten av cellulosaetrar 8,5 x 10"5 - 2,5 x 10"z mol per gram torr cellulosaeter.
13. Förfarande enligt nägot av patentkraven 10 - 12, kän- • * netecknat därav, att när man tvärbinder cellulosaetrar med divinylsulfon, är mängden divinylsulfon 0,005 - 1,5 g 30 / 1 g cellulosaeter.
14. Förfarande enligt patentkravet 10 eller 11, kännetecknat därav, itt när cellulosaetrar graftpolymeriseras 111011 med akrylamid och Ν,Ν'-bismetylenakrylamid, är mängden akrylamid 0,1 - 10 vikt-% (w/v) och mängden Ν,Ν’-bismety-lenakrylamid 0,01 - 10 vikt-% (w/v).
15. Förfarande enligt patentkravet 10, kännetecknat dä-5 rav, att hydrogeler kan bildas av cellulosaetrar genom att graftpolymerisera med friradikalmekanism med akro-lein, akrylamid, 2-akrylamido-2-metylpropansulfonsyra, 2-akrylamido-2-metylpropansulfonsyrans salter, akrylsyra, akrylsyrans salter, akrylonitril, metakrylamid, metakry-10 lamidopropyltrimetylammoniumklorid, metakrylsyra, meta-krylsyrans salter, 2-metakroyloxietansulfonsyra, 2-meta-kroyloxietansulfonsyrans salter, 2-metakryloxietyltrime-tylammoniumklorid, 3-metakryloxi-2-hydroxipropyltrimetyl-ammoniumklorid, metakroylklorid, N,N'-metylenbisakrylamid 15 eller blandningar av dessa.
16. Mikrobiologisk testförpackning, som omfattar ett tor-kat hydrogelodlingsmediumunderlag för mikrober, kanne- / tecknad därav, att hydrogelodlingsmediets gelbildande in- y/ grediens omfattar en hydrogel, som baserar sig pä cellu- 20 losaetrar med kovalenta tvärbindningar, varvid hydrogel-odlingsmediet kan fästas direkt pä ett för vatten ogenomträngligt underlag utan bärare- eller adhesions-skikt.
»♦, 17. Mikrobiologisk testförpackning enligt patentkravet 25 16, kannetecknad därav, att den vidare omfattar en yt- fuktningslösning, en provtagningslösning och en förva-ringsförpackning, som kan fungera som inkubationscell.
18. Användning av hydrogelodlingsmedium enligt patentkra-·. vet 1 för bestämning av arten och mängden av mikrober vid 51 30 olika känslighetsomräden.
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI970163A FI111011B (sv) | 1997-01-15 | 1997-01-15 | Fast tillväxtsunderlag för mikroorganismer, dess framställning och användning |
| HU0000868A HUP0000868A3 (en) | 1997-01-15 | 1998-01-14 | Solid culture medium for microorganisms, process for its preparation, and use |
| CZ19992470A CZ290141B6 (cs) | 1997-01-15 | 1998-01-14 | Suąené hydrogelové kultivační médium pro mikroby, způsob jeho přípravy, testovací mikrobiologická souprava a pouľití |
| PCT/FI1998/000020 WO1998031785A1 (en) | 1997-01-15 | 1998-01-14 | Solid culture medium for microorganisms, process for its preparation, and use |
| PL98334519A PL334519A1 (en) | 1997-01-15 | 1998-01-14 | Solid micro-organism culture medium, method of obtaining same and application thereof |
| AU56639/98A AU722087B2 (en) | 1997-01-15 | 1998-01-14 | Solid culture medium for microorganisms, process for its preparation, and use |
| JP53380398A JP2001507941A (ja) | 1997-01-15 | 1998-01-14 | 微生物用固体培地とその製法および使用 |
| EP98900852A EP0970188A1 (en) | 1997-01-15 | 1998-01-14 | Solid culture medium for microorganisms, process for its preparation, and use |
| US09/093,623 US6258586B1 (en) | 1997-01-15 | 1998-06-01 | Solid culture medium for microorganisms, process for its preparation, and use |
| NO993297A NO993297L (no) | 1997-01-15 | 1999-07-02 | Fast dyrkningsmedium for mikroorganismer, fremgangsmÕte for fremstilling herav og anvendelse herav |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI970163A FI111011B (sv) | 1997-01-15 | 1997-01-15 | Fast tillväxtsunderlag för mikroorganismer, dess framställning och användning |
| FI970163 | 1997-01-15 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI970163A0 FI970163A0 (sv) | 1997-01-15 |
| FI970163A FI970163A (sv) | 1998-07-16 |
| FI111011B true FI111011B (sv) | 2003-05-15 |
Family
ID=8547609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI970163A FI111011B (sv) | 1997-01-15 | 1997-01-15 | Fast tillväxtsunderlag för mikroorganismer, dess framställning och användning |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6258586B1 (sv) |
| EP (1) | EP0970188A1 (sv) |
| JP (1) | JP2001507941A (sv) |
| AU (1) | AU722087B2 (sv) |
| CZ (1) | CZ290141B6 (sv) |
| FI (1) | FI111011B (sv) |
| HU (1) | HUP0000868A3 (sv) |
| NO (1) | NO993297L (sv) |
| PL (1) | PL334519A1 (sv) |
| WO (1) | WO1998031785A1 (sv) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL118376A0 (en) * | 1996-05-22 | 1996-09-12 | Univ Ben Gurion | Polysaccharide sponges for cell culture and transplantation |
| JP4143219B2 (ja) | 1999-05-19 | 2008-09-03 | 日水製薬株式会社 | 簡易培地及びその製造方法 |
| JP4819984B2 (ja) * | 1999-06-23 | 2011-11-24 | 独立行政法人日本原子力研究開発機構 | 自己架橋型アルキルセルロース誘導体、及びそれらの製造方法 |
| AU2001255829A1 (en) | 2000-04-04 | 2001-10-15 | Home Health Science Inc. | Method and kit for detecting microorganisms |
| EP1357179B1 (en) * | 2001-01-29 | 2008-04-23 | Morinaga Milk Industry Co., Ltd. | Solid culture medium and method for preparing the same |
| WO2005083056A1 (ja) * | 2004-03-02 | 2005-09-09 | Independent Administrative Institution, Japan Agency For Marine-Earth Science And Technology | セルロース固体培地とその製造方法 |
| JP2008532551A (ja) * | 2005-03-17 | 2008-08-21 | ファイジェニックス， エルエルシー | 生存可能なレジオネラ属の迅速な検出および定量 |
| WO2007027849A2 (en) * | 2005-08-31 | 2007-03-08 | Board Of Regents, The University Of Texas System | Multiribbon nanocellulose as a matrix for wound healing |
| US8932858B2 (en) * | 2008-03-07 | 2015-01-13 | Corning Incorporated | Modified polysaccharide for cell culture and release |
| JP2010150558A (ja) * | 2010-03-11 | 2010-07-08 | Japan Atomic Energy Agency | 自己架橋型アルキルセルロース誘導体、及びそれらの製造方法 |
| WO2012057751A1 (en) * | 2010-10-27 | 2012-05-03 | Empire Technology Development Llc | Crosslinked cellulosic polymers |
| FI123694B (sv) | 2011-12-22 | 2013-09-30 | Upm Kymmene Corp | Matris och sammansättning för mikrobisk odling av gram-positiva bakterier |
| FI125965B (sv) | 2012-09-25 | 2016-04-29 | Upm Kymmene Corp | Tredimensionel cellodling |
| JP6363326B2 (ja) * | 2013-03-11 | 2018-07-25 | 株式会社コーセー | 酵母・細菌共通培養方法およびこれに用いる酵母・細菌共通培養培地 |
| JP6380003B2 (ja) * | 2014-10-29 | 2018-08-29 | Jnc株式会社 | 微生物培養器材及び微生物検出法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3046201A (en) | 1961-07-17 | 1962-07-24 | American Cyanamid Co | Synthetic culture media |
| US3360440A (en) * | 1966-04-13 | 1967-12-26 | Haab Walter | Cold water reconstitutable microbiological medium, process for preparation and use, ad product |
| CA924224A (en) | 1969-03-10 | 1973-04-10 | A. King Paul | Microbiological testing device and process for preparation |
| US3881993A (en) | 1971-03-16 | 1975-05-06 | Miles Lab | Testing device for microorganisms |
| US3814670A (en) | 1972-04-26 | 1974-06-04 | Miles Lab | Test article for use in microbiology |
| WO1982002563A1 (en) | 1981-01-27 | 1982-08-05 | Minnesota Mining & Mfg | Dry culture media |
| DE3783073T2 (de) | 1986-04-22 | 1993-04-29 | Ajinomoto Kk | Modifiziertes, durch mikroorganismen hergestelltes zellulose-gel und sein komplex mit tierischen zellen. |
| JPH02175532A (ja) | 1988-12-23 | 1990-07-06 | Shinko Seisakusho Co Ltd | 電子写真式プリンタの用紙送り装置 |
| US5089413A (en) | 1989-05-19 | 1992-02-18 | Minnesota Mining And Manufacturing Company | Method and apparatus for culturing with microbiological dry culture medium |
| US5336614A (en) | 1991-08-14 | 1994-08-09 | Quality Biological, Inc. | Soft agar assay and kit |
| US5232838A (en) | 1991-12-09 | 1993-08-03 | Minnesota Mining And Manufacturing Company | Culture media device and method of use |
| JP3146078B2 (ja) * | 1992-10-21 | 2001-03-12 | 島久薬品株式会社 | 微生物を培養する装置 |
| DE4206850C2 (de) | 1992-03-05 | 1996-08-29 | Stockhausen Chem Fab Gmbh | Polymerzusammensetzungen, Herstellgung von Polymerzusammensetzungen, insbesondere Absorptionsmaterialien und deren Verwendung |
| WO1996029428A1 (en) * | 1995-03-20 | 1996-09-26 | Echa Microbiology Limited | Microbial monitoring |
-
1997
- 1997-01-15 FI FI970163A patent/FI111011B/sv not_active IP Right Cessation
-
1998
- 1998-01-14 JP JP53380398A patent/JP2001507941A/ja active Pending
- 1998-01-14 CZ CZ19992470A patent/CZ290141B6/cs not_active IP Right Cessation
- 1998-01-14 WO PCT/FI1998/000020 patent/WO1998031785A1/en not_active Application Discontinuation
- 1998-01-14 EP EP98900852A patent/EP0970188A1/en not_active Withdrawn
- 1998-01-14 PL PL98334519A patent/PL334519A1/xx unknown
- 1998-01-14 AU AU56639/98A patent/AU722087B2/en not_active Ceased
- 1998-01-14 HU HU0000868A patent/HUP0000868A3/hu unknown
- 1998-06-01 US US09/093,623 patent/US6258586B1/en not_active Expired - Fee Related
-
1999
- 1999-07-02 NO NO993297A patent/NO993297L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001507941A (ja) | 2001-06-19 |
| NO993297D0 (no) | 1999-07-02 |
| WO1998031785A1 (en) | 1998-07-23 |
| CZ290141B6 (cs) | 2002-06-12 |
| FI970163A (sv) | 1998-07-16 |
| PL334519A1 (en) | 2000-02-28 |
| CZ247099A3 (cs) | 1999-10-13 |
| HUP0000868A3 (en) | 2002-09-30 |
| AU722087B2 (en) | 2000-07-20 |
| US6258586B1 (en) | 2001-07-10 |
| FI970163A0 (sv) | 1997-01-15 |
| EP0970188A1 (en) | 2000-01-12 |
| NO993297L (no) | 1999-08-24 |
| HUP0000868A2 (en) | 2000-07-28 |
| AU5663998A (en) | 1998-08-07 |
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