FI110365B - Förfarande för producering av mutantproteaser - Google Patents

Förfarande för producering av mutantproteaser Download PDF

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Publication number
FI110365B
FI110365B FI903927A FI903927A FI110365B FI 110365 B FI110365 B FI 110365B FI 903927 A FI903927 A FI 903927A FI 903927 A FI903927 A FI 903927A FI 110365 B FI110365 B FI 110365B
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Finland
Prior art keywords
protease
gene
bacillus
plasmid
strain
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FI903927A
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English (en)
Finnish (fi)
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FI903927A0 (fi
Inventor
Eekelen Christiaan Albertu Van
Der Laan Johannes Cornelis Van
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Genencor Int
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Publication of FI110365B publication Critical patent/FI110365B/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Mushroom Cultivation (AREA)

Claims (15)

1. Förfarande för att producera mutantproteaser, som awi-ker frän ett vildtyp-proteas beträffande ätminstone en ami- 5 nosyra, kännetecknat därav, att man i nämnda förfarande an-vänder som uttryckningsvärd en Bacillus-stam, som inte för-mär producera ett subtilisinenzym.
2. Förfarande enligt patentkravet 1, kännetecknat därav, 10 att Bacillus-stammen förmär producera livsdugliga, värme- beständiga sporer.
3. Förfarande enligt patentkravet 1, kännetecknat därav, att Bacillus-stammen är Spo- och Npr-. 15
4. Förfarande enligt patentkravet 1, kännetecknat därav, att Bacilius-stammen är en alkalofil stam.
5. Förfarande enligt patentkravet 1, kännetecknat därav, ' . ’·· 20 att subtilisingenen har avlägsnats frän genomen.
• · • · · • · · ·.· : 6. Förfarande enligt patentkravet 1, kännetecknat därav, : att Bacillus-stammen är icke-sporbildande. Ml • · · V ' 25
7. Förfarande enligt patentkravet 5, kännetecknat därav, att subtilisingenen har avlägsnats medelst homolog eller • I • I I *· icke-homolog (illegitimate) rekombination. • · • « · • V.·
8. Förfarande enligt patentkravet 1, kännetecknat därav, 30 att Bacilius-stammen är Bacillus novo-art PB92.
» # ♦ ·:··· 9. Förfarande enligt patentkravet 1, kännetecknat därav, att mutantproteaset kodas av en plasmid. 35 1 10365
10. Förfarande enligt patentkravet 1, kännetecknat därav, att mutantproteaset innehäller väsentligen aminosyrasekven-sen av Bacillus novo-art PB92 proteas. 5
11. Förfarande enligt patentkravet 1, kännetecknat därav, att mutantproteaset fogas in i genomen.
12. Förfarande för att erhälla en Bacillus-stam med för-minskad subtilisinnivä, kännetecknat därav, att förfarandet 10 omfattar - isolering av en gen, som kodar för subtilisin, inklu-sive dess 5'- och 31-icke-kodande regioner, infogning av genen i en kloningsvektor, - avlägsning av genens kodande region frän kloningsvek- 15 torn, transformering av en Bacillus-stam med kloningsvek-j torn, - odling av transformanter i förhällanden, i vilka vek-torns replikeringsfunktion är inaktiv, och • · 20. sällning av transformanter, som förlorat den subtili- • # # • · · sin-kodande DNA-sekvensen. • · · • · · • · · • ·
13. Förfarande enligt patentkravet 12, kännetecknat därav, att Bacillus-stammen är alkalofil, företrädesvis Bacillus 25 novo-art PB92. • · • · · * t
* .···[ 14. Bacillus-stam som erhällits enligt patentkravet 12, kännetecknad därav, att den ytterligare innehäller en plas- « I » mid-ekspressionsvektor, som innehäller ett mutantproteas. • · 30
15. Bacillus-stam enligt patentkravet 14, kännetecknad där-' ' av, att den erhällits med homolog eller icke-homolog (ille gitimate) rekombination.
FI903927A 1989-08-11 1990-08-08 Förfarande för producering av mutantproteaser FI110365B (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP89202117 1989-08-11
EP89202117 1989-08-11

Publications (2)

Publication Number Publication Date
FI903927A0 FI903927A0 (fi) 1990-08-08
FI110365B true FI110365B (sv) 2002-12-31

Family

ID=8202457

Family Applications (1)

Application Number Title Priority Date Filing Date
FI903927A FI110365B (sv) 1989-08-11 1990-08-08 Förfarande för producering av mutantproteaser

Country Status (19)

Country Link
US (1) US20040014175A1 (sv)
EP (1) EP0414297B1 (sv)
JP (1) JP3490435B2 (sv)
KR (1) KR0169978B1 (sv)
CN (1) CN1049866A (sv)
AT (1) ATE144283T1 (sv)
AU (1) AU629970B2 (sv)
BG (1) BG60567B1 (sv)
BR (1) BR9003956A (sv)
CA (1) CA2023094C (sv)
DD (1) DD297187A5 (sv)
DE (1) DE69028890T2 (sv)
DK (1) DK0414297T3 (sv)
ES (1) ES2095233T3 (sv)
FI (1) FI110365B (sv)
GR (1) GR3021669T3 (sv)
IE (1) IE77037B1 (sv)
PT (1) PT94961B (sv)
RU (1) RU2060276C1 (sv)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4304161A1 (de) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Verbesserte hochalkalische Proteasen
ATE301719T1 (de) 1994-06-17 2005-08-15 Genencor Int Von der alpha-amylase aus b. licheniformis abgeleitete amylolytische enzyme mit verbesserten eigenschaften
WO1997035956A1 (en) * 1996-03-27 1997-10-02 Novo Nordisk A/S Alkaline protease deficient filamentous fungi
JP3440694B2 (ja) * 1996-05-16 2003-08-25 ライオン株式会社 口腔用組成物
GB0024554D0 (en) 2000-10-06 2000-11-22 Agrol Ltd Ethanol production
EP1495128B1 (en) 2002-03-29 2014-05-07 Genencor International, Inc. Ehanced protein expression in bacillus
ES2547866T3 (es) 2005-02-16 2015-10-09 The General Hospital Corporation Uso de proteínas de fusión de hemojuvelina para regular el metabolismo del hierro mediado por hepcidina
RU2511416C1 (ru) * 2012-10-24 2014-04-10 Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) федеральный университет" (ФГАО ВПО КФУ) Штамм бактерий bacillus pumilus 2a-5 с низкой протеолитической активностью, повышенной активностью фосфатазы, способ его получения и применения
RU2510821C1 (ru) * 2012-10-24 2014-04-10 Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) Федеральный Университет" (ФГАОУ ВПО КФУ) Штамм бактерий bacillus pumilus мк-10 с низкой протеолитической активностью и его применение

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (sv) * 1967-10-03 1971-06-03
JPS6055118B2 (ja) * 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
HUT50877A (en) * 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms
EP0479396B1 (en) * 1987-02-27 1999-06-09 Genencor International, Inc. Transformation of alkalophilic bacillus strains
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用

Also Published As

Publication number Publication date
BG92653A (bg) 1993-12-24
RU2060276C1 (ru) 1996-05-20
AU629970B2 (en) 1992-10-15
ES2095233T3 (es) 1997-02-16
CA2023094A1 (en) 1991-02-12
KR0169978B1 (ko) 1999-02-01
GR3021669T3 (en) 1997-02-28
KR910004805A (ko) 1991-03-29
JP3490435B2 (ja) 2004-01-26
JPH03210177A (ja) 1991-09-13
BR9003956A (pt) 1991-09-03
CN1049866A (zh) 1991-03-13
DK0414297T3 (da) 1997-07-07
US20040014175A1 (en) 2004-01-22
CA2023094C (en) 2000-03-28
PT94961B (pt) 1997-05-28
DE69028890D1 (de) 1996-11-21
IE902904A1 (en) 1991-02-27
FI903927A0 (fi) 1990-08-08
DE69028890T2 (de) 1997-02-27
DD297187A5 (de) 1992-01-02
EP0414297B1 (en) 1996-10-16
PT94961A (pt) 1991-04-18
EP0414297A1 (en) 1991-02-27
BG60567B1 (bg) 1995-08-28
AU6096190A (en) 1991-02-14
IE77037B1 (en) 1997-11-19
ATE144283T1 (de) 1996-11-15

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Owner name: GENENCOR INTERNATIONAL, INC.

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Owner name: GENENCOR INTERNATIONAL, INC.