FI109920B - Immobilisoituja maitohappobakteereja sisältävä bioreaktori ja sen käyttö - Google Patents
Immobilisoituja maitohappobakteereja sisältävä bioreaktori ja sen käyttö Download PDFInfo
- Publication number
- FI109920B FI109920B FI952543A FI952543A FI109920B FI 109920 B FI109920 B FI 109920B FI 952543 A FI952543 A FI 952543A FI 952543 A FI952543 A FI 952543A FI 109920 B FI109920 B FI 109920B
- Authority
- FI
- Finland
- Prior art keywords
- bioreactor
- lactic acid
- reactor
- carrier
- bacteria
- Prior art date
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims description 90
- 239000004310 lactic acid Substances 0.000 title claims description 43
- 235000014655 lactic acid Nutrition 0.000 title claims description 43
- 241000894006 Bacteria Species 0.000 title claims description 39
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 claims description 27
- 238000004519 manufacturing process Methods 0.000 claims description 24
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 15
- 238000011068 loading method Methods 0.000 claims description 12
- 239000001913 cellulose Substances 0.000 claims description 11
- 229920002678 cellulose Polymers 0.000 claims description 11
- 230000001580 bacterial effect Effects 0.000 claims description 9
- 239000011859 microparticle Substances 0.000 claims description 9
- 239000004202 carbamide Substances 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 229920001410 Microfiber Polymers 0.000 claims description 7
- 239000003658 microfiber Substances 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 claims description 5
- 239000004793 Polystyrene Substances 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 229920002223 polystyrene Polymers 0.000 claims description 5
- 108010062877 Bacteriocins Proteins 0.000 claims description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 4
- 230000004060 metabolic process Effects 0.000 claims description 4
- 150000007524 organic acids Chemical class 0.000 claims description 4
- 235000005985 organic acids Nutrition 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- 235000013361 beverage Nutrition 0.000 claims description 2
- 239000001569 carbon dioxide Substances 0.000 claims description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 2
- 230000000887 hydrating effect Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 238000005086 pumping Methods 0.000 claims 2
- 238000001914 filtration Methods 0.000 claims 1
- IQFXJRXOTKFGPN-UHFFFAOYSA-N n-ethenyl-n-ethylethanamine Chemical group CCN(CC)C=C IQFXJRXOTKFGPN-UHFFFAOYSA-N 0.000 claims 1
- 229960000448 lactic acid Drugs 0.000 description 36
- 229920002307 Dextran Polymers 0.000 description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- 238000000034 method Methods 0.000 description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 239000000047 product Substances 0.000 description 10
- 239000000243 solution Substances 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000002028 Biomass Substances 0.000 description 6
- QSJXEFYPDANLFS-UHFFFAOYSA-N Diacetyl Chemical group CC(=O)C(C)=O QSJXEFYPDANLFS-UHFFFAOYSA-N 0.000 description 6
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 description 6
- 108010053775 Nisin Proteins 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 239000004309 nisin Substances 0.000 description 6
- 235000010297 nisin Nutrition 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000012876 carrier material Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- JVTAAEKCZFNVCJ-UWTATZPHSA-N D-lactic acid Chemical compound C[C@@H](O)C(O)=O JVTAAEKCZFNVCJ-UWTATZPHSA-N 0.000 description 4
- 241000186660 Lactobacillus Species 0.000 description 4
- 241000192130 Leuconostoc mesenteroides Species 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229920001600 hydrophobic polymer Polymers 0.000 description 4
- 229940039696 lactobacillus Drugs 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 3
- 241000194036 Lactococcus Species 0.000 description 3
- 239000004367 Lipase Substances 0.000 description 3
- 102000004882 Lipase Human genes 0.000 description 3
- 108090001060 Lipase Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 235000013405 beer Nutrition 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 229940093915 gynecological organic acid Drugs 0.000 description 3
- 235000019421 lipase Nutrition 0.000 description 3
- 235000019626 lipase activity Nutrition 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 108010001682 Dextranase Proteins 0.000 description 2
- 235000008694 Humulus lupulus Nutrition 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241000186713 Lactobacillus amylovorus Species 0.000 description 2
- 244000199866 Lactobacillus casei Species 0.000 description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 244000057717 Streptococcus lactis Species 0.000 description 2
- 235000014897 Streptococcus lactis Nutrition 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000005349 anion exchange Methods 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 229920002301 cellulose acetate Polymers 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000003822 epoxy resin Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000012527 feed solution Substances 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 150000002402 hexoses Chemical class 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 229940017800 lactobacillus casei Drugs 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 229920000647 polyepoxide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- VPSXHKGJZJCWLV-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-3-(1-ethylpiperidin-4-yl)oxypyrazol-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C=1C(=NN(C=1)CC(=O)N1CC2=C(CC1)NN=N2)OC1CCN(CC1)CC VPSXHKGJZJCWLV-UHFFFAOYSA-N 0.000 description 1
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 244000215068 Acacia senegal Species 0.000 description 1
- 241000193798 Aerococcus Species 0.000 description 1
- 241000206594 Carnobacterium Species 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 241000186811 Erysipelothrix Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000193789 Gemella Species 0.000 description 1
- 241000720942 Globicatella Species 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 238000010268 HPLC based assay Methods 0.000 description 1
- 240000001929 Lactobacillus brevis Species 0.000 description 1
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 1
- 241000186679 Lactobacillus buchneri Species 0.000 description 1
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- 241000186840 Lactobacillus fermentum Species 0.000 description 1
- 240000002605 Lactobacillus helveticus Species 0.000 description 1
- 235000013967 Lactobacillus helveticus Nutrition 0.000 description 1
- 241001134654 Lactobacillus leichmannii Species 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 229920000877 Melamine resin Polymers 0.000 description 1
- 241000515012 Micrococcus flavus Species 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- 241000191996 Pediococcus pentosaceus Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102000004879 Racemases and epimerases Human genes 0.000 description 1
- 108090001066 Racemases and epimerases Proteins 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000500334 Tetragenococcus Species 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 241000207194 Vagococcus Species 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 230000004323 axial length Effects 0.000 description 1
- 230000037358 bacterial metabolism Effects 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000012468 concentrated sample Substances 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000009920 food preservation Methods 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- LNEPOXFFQSENCJ-UHFFFAOYSA-N haloperidol Chemical compound C1CC(O)(C=2C=CC(Cl)=CC=2)CCN1CCCC(=O)C1=CC=C(F)C=C1 LNEPOXFFQSENCJ-UHFFFAOYSA-N 0.000 description 1
- JARKCYVAAOWBJS-UHFFFAOYSA-N hexanal Chemical class CCCCCC=O JARKCYVAAOWBJS-UHFFFAOYSA-N 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 1
- 229940012969 lactobacillus fermentum Drugs 0.000 description 1
- 229940054346 lactobacillus helveticus Drugs 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 230000017066 negative regulation of growth Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- HGBOYTHUEUWSSQ-UHFFFAOYSA-N pentanal Chemical class CCCCC=O HGBOYTHUEUWSSQ-UHFFFAOYSA-N 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006068 polycondensation reaction Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002964 rayon Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229940100486 rice starch Drugs 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 235000014214 soft drink Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical compound [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 229940095064 tartrate Drugs 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical class [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 235000019263 trisodium citrate Nutrition 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/78—Cellulose
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4239612 | 1992-11-25 | ||
| DE4239612A DE4239612A1 (de) | 1992-11-25 | 1992-11-25 | Bioreaktor mit immobilisierten, Milchsäure-produzierenden Bakterien und dessen Verwendung in Fermentationsverfahren |
| EP9303285 | 1993-11-23 | ||
| PCT/EP1993/003285 WO1994012614A1 (en) | 1992-11-25 | 1993-11-23 | Bioreactor with immobilized lactic acid bacteria and the use thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI952543A0 FI952543A0 (fi) | 1995-05-24 |
| FI952543A FI952543A (fi) | 1995-07-13 |
| FI109920B true FI109920B (fi) | 2002-10-31 |
Family
ID=6473607
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI952543A FI109920B (fi) | 1992-11-25 | 1995-05-24 | Immobilisoituja maitohappobakteereja sisältävä bioreaktori ja sen käyttö |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US5635368A (es) |
| EP (1) | EP0670890B2 (es) |
| JP (1) | JP3338706B2 (es) |
| KR (1) | KR950704473A (es) |
| AU (1) | AU676000B2 (es) |
| BR (1) | BR9307522A (es) |
| CA (1) | CA2150103A1 (es) |
| DE (2) | DE4239612A1 (es) |
| DK (1) | DK0670890T4 (es) |
| EE (1) | EE9400415A (es) |
| ES (1) | ES2105604T5 (es) |
| FI (1) | FI109920B (es) |
| WO (1) | WO1994012614A1 (es) |
| ZA (1) | ZA938823B (es) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI100338B (fi) * | 1995-07-18 | 1997-11-14 | Danisco Sugar Finland Oy | Menetelmä puhtaan maitohapon valmistamiseksi |
| GB9718415D0 (en) * | 1997-08-29 | 1997-11-05 | Smithkline Beecham Plc | Formulation |
| DE19848623A1 (de) * | 1998-10-21 | 2000-04-27 | Cultor Corp | Preiswerte Immobilisierungsmatrizen aus natürlichen Materialien |
| PL203207B1 (pl) | 1999-03-11 | 2009-09-30 | Zeachem Inc | Sposób wytwarzania etanolu |
| US20030108980A1 (en) * | 2000-08-14 | 2003-06-12 | Sayler Gary S. | Bioluminescent methods for direct visual detection of environmental compounds |
| MXPA03004562A (es) * | 2000-11-22 | 2003-09-10 | Univ Maryland | Uso de hemolisina de clya pra la excrecion de proteinas. |
| FI20002792A0 (fi) * | 2000-12-20 | 2000-12-20 | Hydrios Biotechnology Oy | Menetelmä D-mannitolin tuottamiseksi |
| CN1492922A (zh) * | 2001-02-23 | 2004-04-28 | 乔治亚大学研究基金公司 | 用于澄清啤酒和葡萄酒发酵的纤维惰性载体 |
| JP2003061695A (ja) * | 2001-08-24 | 2003-03-04 | Kuraudo:Kk | 生ゴミ等から有用物質を生産する方法 |
| US7875337B2 (en) * | 2003-01-24 | 2011-01-25 | Glastic Corporation | Fiber and resin composite reinforcement |
| US20060141584A1 (en) * | 2004-12-28 | 2006-06-29 | David Litzen | Pretreatment of biomass for ethanol production |
| US20060293261A1 (en) * | 2005-06-24 | 2006-12-28 | Taisho Pharmaceutical Co., Ltd. | Clarithromycin or a salt thereof for the treatment or prevention of pulmonary disorders caused by the destruction of pulmonary alveoli |
| US9051574B2 (en) | 2008-06-03 | 2015-06-09 | University Of Maryland, Baltimore | Non-hemolytic ClyA for excretion of proteins |
| US8398856B2 (en) | 2011-06-10 | 2013-03-19 | West Bengal University Of Technology | Self-sustained microbial detoxification of soluble sulfate from environmental effluent |
| WO2014031842A1 (en) | 2012-08-22 | 2014-02-27 | Kraft Foods Group Brands Llc | Processed cheese with cultured dairy components and method of manufacturing |
| KR200484770Y1 (ko) * | 2016-04-12 | 2017-11-21 | 전남대학교산학협력단 | 글루칸수크라아제 생산 균주 고정화 담체의 제조 방법, 이에 의하여 제조된 글루칸수크라아제 생산 균주 고정화 담체 및 이를 이용한 올리고당 생산 시스템 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4063017A (en) * | 1976-04-22 | 1977-12-13 | Purdue Research Foundation | Porous cellulose beads and the immobilization of enzymes therewith |
| US4153510A (en) † | 1977-09-14 | 1979-05-08 | Corning Glass Works | High surface low volume biomass composite |
| GB2006181B (en) † | 1977-10-20 | 1982-05-19 | Hartley Simon Ltd | Growth of biological material |
| US4355117A (en) * | 1980-10-08 | 1982-10-19 | Nabisco Brands, Inc. | Process for preparing agglomerated fibrous cellulose |
| CA1161198A (en) * | 1980-10-08 | 1984-01-24 | Richard L. Antrim | Process for preparing agglomerated fibrous cellulose |
| DE3247214A1 (de) * | 1982-12-18 | 1984-06-20 | Hoechst Ag, 6230 Frankfurt | Reaktionen mit immobilisierten biokatalysatoren |
| DE3410650A1 (de) † | 1984-03-23 | 1985-10-03 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | Mit mikroorganismen bewachsene poroese anorganische traeger, verfahren zur immobilisierung von mikroorganismen und dafuer geeignete traegerkoerper |
| US4794080A (en) * | 1984-04-16 | 1988-12-27 | Igene Biotechnology, Inc. | Microbial co-culture production of propionic acid |
| JPS61149085A (ja) * | 1984-12-24 | 1986-07-07 | Chiyoda Chem Eng & Constr Co Ltd | 微生物担持体 |
| SE455103B (sv) † | 1985-01-09 | 1988-06-20 | Lars Edgar Martin Ehrnford | Berare for immobilisering av biologiskt aktivt organiskt material, vilken utgores av sammanfogade partiklar av en poros sintrad glasfibermatris |
| US4814273A (en) * | 1985-03-20 | 1989-03-21 | Michigan Biotechnology Institute | Production of organic acids by an improved fermentation process |
| IL75554A (en) * | 1985-06-18 | 1993-01-14 | Yeda Res & Dev | Matrix for cell cultivation in vitro |
| US5037749A (en) * | 1986-07-08 | 1991-08-06 | Protein Foods Group Inc. | Porous immobilization support prepared from animal bone |
| EP0280398B1 (en) * | 1987-02-06 | 1993-06-30 | NAGASE & COMPANY, LTD. | Method for producing acid urease, and use thereof |
| US4867992A (en) * | 1987-03-16 | 1989-09-19 | General Foods Corporation | Natural coffee flavor by fermentation |
| US4929452A (en) * | 1987-09-30 | 1990-05-29 | University Of Georgia Research Foundation, Inc. | Method for rapidly fermenting alcoholic beverages |
| US4942032A (en) * | 1988-09-23 | 1990-07-17 | Microlife Technics, Inc. | Process for producing a novel antifungal product |
| US5079011A (en) * | 1988-09-27 | 1992-01-07 | Cultor, Ltd. | Method using immobilized yeast to produce ethanol and alcoholic beverages |
| JPH0312866A (ja) * | 1989-06-09 | 1991-01-21 | Fujitsu Ltd | 書込読出動作の開始制御方式 |
| JP2901008B2 (ja) * | 1989-11-28 | 1999-06-02 | 明治乳業株式会社 | 乳酸菌によるジアセチル、アセトイン発酵法 |
| FR2665826A1 (fr) * | 1990-08-16 | 1992-02-21 | Kabore Paul | Procede pour l'obtention de boissons fermentees stabilisees type kefir et boissons ainsi obtenues. |
-
1992
- 1992-11-25 DE DE4239612A patent/DE4239612A1/de not_active Withdrawn
-
1993
- 1993-11-23 DE DE69311134T patent/DE69311134T3/de not_active Expired - Fee Related
- 1993-11-23 JP JP51275094A patent/JP3338706B2/ja not_active Expired - Fee Related
- 1993-11-23 BR BR9307522-7A patent/BR9307522A/pt not_active Application Discontinuation
- 1993-11-23 WO PCT/EP1993/003285 patent/WO1994012614A1/en active IP Right Grant
- 1993-11-23 EP EP94901854A patent/EP0670890B2/en not_active Expired - Lifetime
- 1993-11-23 DK DK94901854T patent/DK0670890T4/da active
- 1993-11-23 AU AU56267/94A patent/AU676000B2/en not_active Ceased
- 1993-11-23 ES ES94901854T patent/ES2105604T5/es not_active Expired - Lifetime
- 1993-11-23 KR KR1019950702102A patent/KR950704473A/ko not_active Application Discontinuation
- 1993-11-23 CA CA002150103A patent/CA2150103A1/en not_active Abandoned
- 1993-11-24 US US08/157,759 patent/US5635368A/en not_active Expired - Fee Related
- 1993-11-25 ZA ZA938823A patent/ZA938823B/xx unknown
-
1994
- 1994-11-22 EE EE9400415A patent/EE9400415A/xx unknown
-
1995
- 1995-05-24 FI FI952543A patent/FI109920B/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ZA938823B (en) | 1994-06-30 |
| FI952543A0 (fi) | 1995-05-24 |
| DK0670890T4 (da) | 2000-12-04 |
| DE69311134D1 (de) | 1997-07-03 |
| BR9307522A (pt) | 1999-08-31 |
| CA2150103A1 (en) | 1994-06-09 |
| JPH08503375A (ja) | 1996-04-16 |
| AU5626794A (en) | 1994-06-22 |
| EP0670890B2 (en) | 2000-02-02 |
| KR950704473A (ko) | 1995-11-20 |
| AU676000B2 (en) | 1997-02-27 |
| ES2105604T5 (es) | 2000-06-16 |
| EP0670890B1 (en) | 1997-05-28 |
| DE4239612A1 (de) | 1994-05-26 |
| FI952543A (fi) | 1995-07-13 |
| JP3338706B2 (ja) | 2002-10-28 |
| WO1994012614A1 (en) | 1994-06-09 |
| EP0670890A1 (en) | 1995-09-13 |
| US5635368A (en) | 1997-06-03 |
| DE69311134T3 (de) | 2000-07-06 |
| EE9400415A (et) | 1996-06-17 |
| DK0670890T3 (da) | 1997-12-15 |
| DE69311134T2 (de) | 1997-10-09 |
| ES2105604T3 (es) | 1997-10-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FI109920B (fi) | Immobilisoituja maitohappobakteereja sisältävä bioreaktori ja sen käyttö | |
| Kosseva et al. | Use of immobilised biocatalysts in the processing of cheese whey | |
| KR101411073B1 (ko) | 폴리비닐 알코올 겔 내에 고정화된 효소 또는 미생물 형태의 생체촉매의 산업적 생산 방법, 이의 사용 방법, 및 이의 생산 장치 | |
| Jagannath et al. | Comparative evaluation of bacterial cellulose (nata) as a cryoprotectant and carrier support during the freeze drying process of probiotic lactic acid bacteria | |
| Silva et al. | Kinetics and stability of a fibrous-bed bioreactor for continuous production of lactic acid from unsupplemented acid whey | |
| Meleigy et al. | Biosynthesis of gibberellic acid from milk permeate in repeated batch operation by a mutant Fusarium moniliforme cells immobilized on loofa sponge | |
| US20050019894A1 (en) | Novel lactobacillus sp. strain and use thereof | |
| Ho et al. | Ingredient selection for plastic composite supports for L-(+)-lactic acid biofilm fermentation by Lactobacillus casei subsp. rhamnosus | |
| CN105154371B (zh) | 一株产阿魏酸酯酶的噬淀粉乳杆菌及其应用 | |
| CN101392223A (zh) | 一种微生物饲料添加剂菌种的选育方法 | |
| CN110804571B (zh) | 一种复合乳酸菌制剂及在制备成饲料添加剂中的应用 | |
| CN108841757A (zh) | 一种乳酸细菌菌种保藏方法 | |
| KR102675187B1 (ko) | 락토바실러스 플란타룸 kcc-24 및 이를 포함하는 조성물 | |
| CN111575213A (zh) | 一种微生物复合菌剂及其在制备发酵饲料中的应用 | |
| CN111676170B (zh) | 一株发酵乳杆菌及其在制备共轭脂肪酸中的应用 | |
| CN113943667B (zh) | 一株分离自骆驼瘤胃的植物乳杆菌及其在青贮中的应用 | |
| CZ303986B6 (cs) | Prípravek obsahující probiotickou kulturu, zpusob jeho výroby a pouzití | |
| Krisch et al. | Application of immobilized cells and enzymes in the food industry | |
| CN117918528A (zh) | 一种肠道保护用具有益生菌的保健品及其制备方法 | |
| Moneta et al. | Suitability of Lactobacillus strains as components of probiotics | |
| Zuriana et al. | Production of sorbitol by repeated batch fermentation using immobilized of Lactobacillus plantarum strain (BAA-793) via solid state fermentation | |
| JP2000245491A (ja) | 高純度ｌ乳酸の製造方法 | |
| CN113755335A (zh) | 一种能解酒的菌株的筛选方法及应用 | |
| Berghofer et al. | Session II: Posters |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MA | Patent expired |