FI104638B - Förfarande för framställning av ett flagellinfusionsprotein, en gen som kodar det och en mikroorganism - Google Patents
Förfarande för framställning av ett flagellinfusionsprotein, en gen som kodar det och en mikroorganism Download PDFInfo
- Publication number
- FI104638B FI104638B FI905441A FI905441A FI104638B FI 104638 B FI104638 B FI 104638B FI 905441 A FI905441 A FI 905441A FI 905441 A FI905441 A FI 905441A FI 104638 B FI104638 B FI 104638B
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- Prior art keywords
- flagellin
- epitope
- recombinant
- gene
- protein
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Classifications
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
- C07K14/445—Plasmodium
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- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
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- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
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- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Claims (21)
1. Förfarande för framställning av en rekombinant gen, vilken omfattar en nukleotidsekvens sora kodar för ett 5 flagellinfusionsprotein, vilket omfattar en första epitop sora kodas av en Salmonella eller Shigella flagellin-struk-turgen ooh ätminstone en epitop av en heterolog organism, vilken är en organism som skiljer sig frän den organism frän vilken den första epitopen härstammar och vilken epi- 10 top inte väsentligen är identisk med en epitop som kodas av en flagellingen, och flagellinfusionsproteinet förmär sammangä tili ett funktionellt flagellum, och den väsent-liga antigeniciteten hos proteinets flagellindel bibe-hälls, kännetecknat av att man 15 a) isolerar flagellingenens sekvenser b) isolerar sekvenser som kodar den heterologa or-ganismens epitop(er), och c) konstruerar nämnda rekombinanta gen, genom att införa sekvensen eller sekvenserna som kodar den heterolo- 20 ga organismens epitop(er) i flagellingenens sekvenser el ler ersätta dem med sekvenser som kodar den heterologa organismens epitop(er).
2. Förfarande enligt patentkrav 1, kännetecknat av att flagellinstrukturgenen härstammar ·· 25 frän Salmonella Hl-, Hl-d- eller H2-genen.
3. Förfarande enligt patentkrav 1, kännetecknat av att den heterologa epitopen är immunogen vid införandet av fusionsproteinet i en värd ur ordningen ryggradsdjur, och att den immunogena epitopen 30 framkallar ett immunsvar, vilket är av T-cell-, B-cell- eller cellulär natur eller kombinationer av dessa.
4. Förfarande enligt patentkrav 1, kännetecknat av att den heterologa organismen är en parasit, en bakterie, ett virus eller en svamp. 35 5. Förfarande enligt patentkrav 4, k ä n n e - j tecknatav att epitopen av den heterologa organis- 93 104638 men är en epitop av en circumsporozoitproteinantigen, ett Streptococcus M-protein, koleratoxin B-underenhet, hepatit B-ytanantigenen eller hepatit B-preytantigenen, HIV-skalp-roteinet eller rotavirus VP7-protein.
5
6. Förfarande enligt patentkrav 3, kanne- tecknat av att epitopen är en T-cell-epitop, B-cell-epitop eller en kombination av dessa.
7. Förfarande enligt patentkrav 6, kanne-tecknat av att T-cell-epitopen härstammar frän 10 difteri CRMI97-toxinet 366-383.
8. Förfarande enligt patentkrav 1, kanne-tecknat av att man framstaller en rekombinant gen som omfattar en Salmonella flagellinstrukturgen med en heterolog DNA-sekvens som kopplats tili flagellinstruktur- 15 genen i en region, vilken ej är väsentlig för funktionen hos det kodade flagellinet.
9. Förfarande enligt patentkrav 8, k ä n n e -tecknat av att den heterologa DNA-sekvensen kopplats i den hypervariabla regionen av flagellinstrukturge- 20 nen.
10. Förfarande enligt patentkrav 8, k ä n n e -tecknat av att flagellinstrukturgenen härstammar frän Salmonella Hl-, Hl-d- eller H2-genen.
11. Förfarande enligt patentkrav 10, k ä n n e - ••'25 tecknat av att den heterologa DNA-sekvensen kopp lats mellan de naturliga EcoRV-ställena i Salmonella Hl-d-genen.
12. Förfarande enligt patentkrav 8, k ä n n e -tecknat av att den heterologa DNA-sekvensen kodar 30 en epitop av en organism, vilken är patogen för ryggradsd- jur. • · ·
13. Förfarande för framställning av en rekombinant nukleinsyraklon, vilken innehäller den rekombinanta genen enligt patentkrav l, kännetecknat av att den 35 rekombinanta genen enligt patentkrav 1 införs i en vektor 94 104638 och en klon, som innehäller nämnda rekombinanta gen, iso-leras.
14. Förfarande enligt patentkrav 13, kanne-tecknat av att man framställer en rekombinant nuk- 5 leinsyraklon, som innehäller en plasmid som är pPX1653, pPX1662, pLS411 eller pROTA92-19, vilka deponerats vid ATCC och tilldelats deponeringsnummer 67688, 67687, 67686 respektive 67945.
15. Förfarande för framställning av en rekombinant 10 mikroorganism, som omfattar den rekombinanta genen enligt patentkrav 1, kännetecknat av att en lämplig vektor, som innehäller den rekombinanta genen enligt patentkrav 1, införs i en mikroorganism.
16. Forfarande enligt patentkrav 15, k ä n n e - 15 tecknatav att den rekombinanta mikroorganismen är en försvagad bakterie, som förmär tränga in i kroppen och den är Salmonella, Shigella och Escherichia coli.
17. Förfarande för framställning av ett fusionspro-tein, som omfattar en första epitop som kodas av en Salmo- 20 neliä eller Shigella flagellinstrukturgen och ätminstone en epitop av en heterolog organism, vilken är en organism som skiljer sig frän den organism frän vilken den första epitopen härstammar och vilken epitop inte väsentligen är identisk med en epitop som kodas ev en flagellingen och ;·· 25 den väsentliga antigeniciteten hos proteinets flagellindel bibehälls, kännetecknat av att man a) inför en gen enligt nägot av patentkraven 1-12 i en lämplig expressionsvektor, b) inför vektorn i en lämplig värdcell och expres- 30 serar den där, och ’* c) tar tillvara det expresserade flagellinfusions- • · . proteinet.
18. Förfarande enligt patentkrav 17, kännetecknat av att man framställer ett fusionsprotein, 35 väri epitopen av den heterologa organismen är immunogen ! 104638 95 vid införandet av proteinet i en värd ur ordningen ryg-gradsdjur, varvid den immunogena epitopen framkallar ett immunsvar av T-cell-, B-cell- eller cellular natur.
19. Förfarande enligt patentkrav 17, kanne-5 tecknat av att man framställer ett fusionsprotein som omfattar ett Salmonella flagellinprotein med en hete-rolog aminosyrasekvens, vilken kopplats tili i flagellin-proteinet i en region, vilken ej är väsentlig för funktio-nen hos flagellinproteinet. 10
20. Förfarande enligt patentkrav 19, kanne - tecknat av att man framställer ett fusionsprotein där den heterologa aminosyrasekvensen är en epitop som är immunogen vid införandet av proteinet i en värd av ordningen ryggradsdjur, varvid den immunogena epitopen framkal-15 lar ett B-cell-, T-cell- eller cellulärt gensvar eller en kombination av dessa.
21. Förfarande för expression av ett rekombinant flagellinfusionsprotein, kännetecknat av att man 20 a) konstruerar en rekombinant gen, vilken omfattar en nukleotidsekvens som kodar ett Salmonella flagellin-fusionsprotein, vilket omfattar en första epitop som kodas av en flagellinstrukturgen och ätminstone en epitop av en heterolog organism, vilken är en organism som skiljer sig *·' 25 frän den organism frän vilken den första epitopen härstam- mar, och vilken epitop inte väsentligen är identisk med en epitop som kodas av en flagellingen, och den väsentliga antigeniciteten hos proteinets flagellindel bibehälls b) inför den rekombinanta genen i en lämplig ex-30 pressionsvektor; ' " c) insätter vektorn i en lämplig värd; och • · . d) tilläter att den bakteriella värden, vilken in-nehäller vektorn, att föröka sig under förhällanden som inducerar expression av den rekombinanta genen.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19057088A | 1988-05-05 | 1988-05-05 | |
| US19057088 | 1988-05-05 | ||
| US8901932 | 1989-05-05 | ||
| PCT/US1989/001932 WO1989010967A1 (en) | 1988-05-05 | 1989-05-05 | Recombinant flagellin vaccines |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FI905441A0 FI905441A0 (fi) | 1990-11-02 |
| FI104638B true FI104638B (sv) | 2000-03-15 |
Family
ID=22701885
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI905441A FI104638B (sv) | 1988-05-05 | 1990-11-02 | Förfarande för framställning av ett flagellinfusionsprotein, en gen som kodar det och en mikroorganism |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0419513B1 (sv) |
| JP (1) | JP2793673B2 (sv) |
| AT (1) | ATE121782T1 (sv) |
| AU (1) | AU637049B2 (sv) |
| CA (1) | CA1340817C (sv) |
| DE (1) | DE68922394T2 (sv) |
| DK (1) | DK263390A (sv) |
| FI (1) | FI104638B (sv) |
| NO (1) | NO302031B1 (sv) |
| WO (1) | WO1989010967A1 (sv) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5698390A (en) | 1987-11-18 | 1997-12-16 | Chiron Corporation | Hepatitis C immunoassays |
| US6171782B1 (en) | 1987-11-18 | 2001-01-09 | Chiron Corporation | Antibody compositions to HCV and uses thereof |
| US5712088A (en) * | 1987-11-18 | 1998-01-27 | Chiron Corporation | Methods for detecting Hepatitis C virus using polynucleotides specific for same |
| US6861212B1 (en) | 1987-11-18 | 2005-03-01 | Chiron Corporation | NANBV diagnostics and vaccines |
| US5714596A (en) * | 1987-11-18 | 1998-02-03 | Chiron Corporation | NANBV diagnostics: polynucleotides useful for screening for hepatitis C virus |
| US7118757B1 (en) | 1988-12-19 | 2006-10-10 | Wyeth Holdings Corporation | Meningococcal class 1 outer-membrane protein vaccine |
| ES2070312T5 (es) * | 1988-12-19 | 2003-05-16 | American Cyanamid Co | Vacuna de proteina de membrana exterior meningococica de clase 1. |
| US6027729A (en) * | 1989-04-20 | 2000-02-22 | Chiron Corporation | NANBV Diagnostics and vaccines |
| DE69126786T2 (de) * | 1990-10-01 | 1998-01-08 | Mini Agriculture & Fisheries | Polynukleotidsequenz von salmonella |
| EP0551325B1 (en) * | 1990-10-01 | 2000-03-15 | The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Gvt. Of The U. K. Of Great Britain And N. Ireland | Method of testing for salmonella |
| GB9101550D0 (en) * | 1991-01-24 | 1991-03-06 | Mastico Robert A | Antigen-presenting chimaeric protein |
| IL101639A0 (en) * | 1992-04-17 | 1992-12-30 | Yeda Res & Dev | Recombinant influenza vaccines |
| DK1112747T3 (da) * | 1999-12-28 | 2004-10-25 | Akzo Nobel Nv | Salmonellavaccine, som ikke inducerer antistoffer mod flagellin eller flageller |
| AU2002359984A1 (en) * | 2002-12-16 | 2004-07-09 | Caf Laboratories Inc. | Salmonella antigen formulation and antibody test kit and subunit vaccine using the same |
| US20080248068A1 (en) | 2004-05-07 | 2008-10-09 | Hans-Gustaf Ljunggren | Use of Flagellin as an Adjuvant for Vaccine |
| AU2005323811B2 (en) * | 2004-12-02 | 2012-07-05 | Csir | Gram positive bacterial cells comprising a disrupted flagellin gene, flagellin-based fusion proteins and use in removal of metal ions from a liquid |
| CA2589553C (en) | 2004-12-16 | 2014-02-18 | Wake Forest University Health Sciences | Use of flagellin in the immunotherapy of yersinia pestis |
| AR052195A1 (es) | 2005-01-19 | 2007-03-07 | Vaxinnate Corp | Composiciones de patrones moleculares asociados a patogenos en metodos de uso |
| ES2555544T3 (es) | 2006-03-07 | 2016-01-04 | Vaxinnate Corporation | Composiciones que incluyen hemaglutinina, métodos de preparación y métodos de uso de las mismas |
| WO2009128949A2 (en) | 2008-04-18 | 2009-10-22 | Vaxinnate Corporation | Compositions of dengue viral proteins and methods of use |
| US8932598B2 (en) | 2012-08-28 | 2015-01-13 | Vaxinnate Corporation | Fusion proteins and methods of use |
| CN102816246B (zh) * | 2012-09-04 | 2014-07-23 | 成都蓉生药业有限责任公司 | 一种人巨细胞病毒免疫原融合蛋白及其制备方法和用途 |
| JP2017530114A (ja) | 2014-09-26 | 2017-10-12 | バヴァリアン・ノルディック・アクティーゼルスカブ | フラジェリンをコードする組み換えｍｖａによる鼻腔内免疫のための方法と組成物 |
| WO2016081619A1 (en) * | 2014-11-18 | 2016-05-26 | The Trustees Of Columbia University In The City Of New York | Detection of analytes using live cells |
| CN104402974B (zh) * | 2014-12-10 | 2017-11-14 | 重庆医科大学 | 一种具有粘膜免疫佐剂活性的多肽及其在制备粘膜免疫佐剂中的用途 |
| US10849938B2 (en) | 2017-09-13 | 2020-12-01 | ZBiotics Company | Gene expression system for probiotic microorganisms |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4735801A (en) * | 1982-09-07 | 1988-04-05 | Board Of Trustees Of Leland Stanford Jr. University | Novel non-reverting salmonella live vaccines |
| EP0241546A4 (en) * | 1985-10-11 | 1988-11-02 | Genetics Inst | METHOD FOR PRODUCING HETEROLOGICAL PROTEINS. |
| EP0237045B1 (en) * | 1986-03-11 | 1993-10-20 | Shionogi & Co., Ltd. | DNA encoding flagellin and vector having the same |
| CA1331355C (en) * | 1986-04-21 | 1994-08-09 | Bioenterprises Pty. Ltd | Immunopotentation |
-
1989
- 1989-05-05 DE DE68922394T patent/DE68922394T2/de not_active Expired - Fee Related
- 1989-05-05 WO PCT/US1989/001932 patent/WO1989010967A1/en active IP Right Grant
- 1989-05-05 AT AT89906507T patent/ATE121782T1/de not_active IP Right Cessation
- 1989-05-05 CA CA000598847A patent/CA1340817C/en not_active Expired - Fee Related
- 1989-05-05 AU AU36979/89A patent/AU637049B2/en not_active Ceased
- 1989-05-05 JP JP1505981A patent/JP2793673B2/ja not_active Expired - Fee Related
- 1989-05-05 EP EP89906507A patent/EP0419513B1/en not_active Expired - Lifetime
-
1990
- 1990-11-02 DK DK263390A patent/DK263390A/da not_active Application Discontinuation
- 1990-11-02 FI FI905441A patent/FI104638B/sv not_active IP Right Cessation
- 1990-11-05 NO NO904806A patent/NO302031B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU637049B2 (en) | 1993-05-20 |
| NO302031B1 (no) | 1998-01-12 |
| ATE121782T1 (de) | 1995-05-15 |
| JPH04502402A (ja) | 1992-05-07 |
| FI905441A0 (fi) | 1990-11-02 |
| DK263390D0 (da) | 1990-11-02 |
| DE68922394T2 (de) | 1995-10-05 |
| CA1340817C (en) | 1999-11-09 |
| NO904806L (no) | 1991-01-03 |
| WO1989010967A1 (en) | 1989-11-16 |
| EP0419513B1 (en) | 1995-04-26 |
| NO904806D0 (no) | 1990-11-05 |
| DE68922394D1 (de) | 1995-06-01 |
| EP0419513A1 (en) | 1991-04-03 |
| DK263390A (da) | 1991-01-04 |
| JP2793673B2 (ja) | 1998-09-03 |
| AU3697989A (en) | 1989-11-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GB | Transfer or assigment of application |
Owner name: AMERICAN CYANAMID COMPANY |
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| FG | Patent granted |
Owner name: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIO Owner name: AMERICAN CYANAMID COMPANY |
|
| MA | Patent expired |