ES8802537A1 - Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado. - Google Patents
Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado.Info
- Publication number
- ES8802537A1 ES8802537A1 ES557676A ES557676A ES8802537A1 ES 8802537 A1 ES8802537 A1 ES 8802537A1 ES 557676 A ES557676 A ES 557676A ES 557676 A ES557676 A ES 557676A ES 8802537 A1 ES8802537 A1 ES 8802537A1
- Authority
- ES
- Spain
- Prior art keywords
- fibrinolytic enzyme
- modified
- region
- growth factor
- plasminogen activator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
PROCEDIMIENTO PARA PREPARAR UNA CELULA HUESPED TRANSFORMADA CON UN VECTOR DE EXPRESION CORRESPONDIENTE A UN ACTIVANTE DE PLASMINOGENO DE TEJIDO MODIFICADO, EN EL QUE EL VECTOR ES DE EXPRESION REPLICABLE CAPAZ, EN UNA CELULA HUESPED, DE EXPRESAR UN POLIMERO DE ADN QUE COMPRENDE UNA SECUENCIA NUCLEOTIDA QUE CODIFICA UN ACTIVADOR DE PLAMINOGENO DE TIPO TEJIDO FIBRINOLITICAMENTE ACTIVO QUE HA SIDO MODIFICADO EN LA REGION DEL DOMINIO DEL FACTOR DE CRECIMIENTO. SE TRANSFORMA UNA CELULA HUESPED CON DICHO VECTOR BAJO CONDICIONES TRANSFORMANTES EN UN AMBIENTE TRANSFORMANTE, MEDIANTE TRATAMIENTO DE UNA CELULA HUESPED BACTERIANA CON UNA SOLUCION DE CAC12 O DE UNA MEZCLA DE RBC1, MNC12, ACETATO DE POTASIO Y GLICEROL, Y DESPUES, CON ACIDO 3-SIGMAN-MORFOLINOPROPANO SULFONICO, RBC1 Y GLICEROL, O MEDIANTE CO-PRECIPITACION CON CALCIO DEL VECTOR DE ADN SOBRE UNA CELULA HUESPED DE MAMIFERO. SE APLICA EN COMPOSICIONES FARMACEUTICAS PARA EL TRATAMIENTO DE ENFERMEDADES TROMBOTICAS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB858508972A GB8508972D0 (en) | 1985-04-04 | 1985-04-04 | Compounds |
GB868604852A GB8604852D0 (en) | 1986-02-27 | 1986-02-27 | Compounds |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8802537A1 true ES8802537A1 (es) | 1988-07-16 |
ES557676A0 ES557676A0 (es) | 1988-07-16 |
Family
ID=26289098
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES553651A Expired ES8801374A1 (es) | 1985-04-04 | 1986-04-02 | Un procedimiento para preparar un activante de plasminogeno. |
ES557675A Expired ES8801551A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un vector de expresion replicable. |
ES557674A Expired ES8802442A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un polimero de adn. |
ES557677A Expired ES8801552A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa. |
ES557676A Expired ES8802537A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado. |
Family Applications Before (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES553651A Expired ES8801374A1 (es) | 1985-04-04 | 1986-04-02 | Un procedimiento para preparar un activante de plasminogeno. |
ES557675A Expired ES8801551A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un vector de expresion replicable. |
ES557674A Expired ES8802442A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un polimero de adn. |
ES557677A Expired ES8801552A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0207589A1 (es) |
DK (1) | DK149786A (es) |
ES (5) | ES8801374A1 (es) |
GR (1) | GR860875B (es) |
NZ (1) | NZ215660A (es) |
PT (1) | PT82326B (es) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2053449T3 (es) * | 1985-12-20 | 1994-08-01 | Upjohn Co | Analogos (tpa) de activadores de plasminogeno de tejido. |
US5106741A (en) * | 1985-12-20 | 1992-04-21 | The Upjohn Company | Tissue plasminogen activator (TPA) analogs |
US5002887A (en) * | 1986-01-31 | 1991-03-26 | Genetics Institute, Inc. | Truncated thrombolytic proteins |
US5837518A (en) * | 1986-01-31 | 1998-11-17 | Genetics Institute, Inc. | Thrombolytic proteins |
US5258298A (en) * | 1986-01-31 | 1993-11-02 | Genetics Institute, Inc. | Deletion and glycosylation mutant of human tissue plasminogen activator |
DE3682891D1 (de) * | 1986-02-17 | 1992-01-23 | Stichting Centraal Lab | Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen. |
NZ219829A (en) * | 1986-04-02 | 1990-05-28 | Beecham Group Plc | Tissue-type plasminogen activator modified in the region of amino acids 67-69 and pharmaceutical compositions thereof |
PT84589B (en) * | 1986-04-02 | 1989-05-09 | Beecham Group Plc | Process for preparing a fibrinolytic enzyme |
AU7090887A (en) * | 1986-04-02 | 1987-10-08 | Beecham Group Plc | Plasminogen activators modified in first kringle domain |
DE3643158A1 (de) * | 1986-04-21 | 1987-11-19 | Boehringer Mannheim Gmbh | Gewebs-plasminogen-aktivator(tpa) - derivat und seine herstellung |
EP0266032A1 (en) * | 1986-08-29 | 1988-05-04 | Beecham Group Plc | Modified fibrinolytic enzyme |
US5580559A (en) * | 1986-12-05 | 1996-12-03 | Ciba-Geigy Corporation | Hybrid plasminogen activator |
US5242819A (en) * | 1986-12-05 | 1993-09-07 | Ciba-Geigy Corporation | DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase |
GB8701811D0 (en) * | 1987-01-28 | 1987-03-04 | Beecham Group Plc | Compounds |
WO1988005822A1 (en) * | 1987-01-30 | 1988-08-11 | American Home Products Corporation | Des-epidermal growth factor plasminogen activators |
NL8701021A (nl) * | 1987-04-29 | 1988-11-16 | Stichting Centraal Lab | Humane t-pa(u-pa) substitutie-mutant eiwitten, daarvoor coderend recombinant dna, getransfecteerde gastheercellen, bereiding van de mutant eiwitten, en farmaceutische preparaten. |
DE3853100T2 (de) * | 1987-06-04 | 1995-08-03 | Zymogenetics Inc | Gewebs-Plasminogen-Aktivator-Analoge mit modifizierten Wachstumsfaktordomänen. |
ATE110772T1 (de) * | 1987-06-04 | 1994-09-15 | Zymogenetics Inc | Mutierter t-pa mit kringle-ersatz. |
SE8702562L (sv) * | 1987-06-18 | 1988-12-19 | Kabigen Ab | Nya fibrinolytiska enzymer |
US4935237A (en) * | 1988-03-21 | 1990-06-19 | Genentech, Inc. | Processes for the preparation of t-PA mutants |
AU613942B2 (en) * | 1987-06-30 | 1991-08-15 | Genentech Inc. | Improved processes for the treatment of vascular disease |
JPH02504102A (ja) * | 1987-06-30 | 1990-11-29 | ジェネンテク,インコーポレイテッド | 血管障害治療のための改良法 |
JPH03500002A (ja) * | 1987-07-06 | 1991-01-10 | ジェネティックス・インスチチュート・インコーポレーテッド | 新規な血栓崩壊蛋白 |
GB8721023D0 (en) * | 1987-09-07 | 1987-10-14 | Lepetit Spa | Modified plasminogen activators |
US5244676A (en) * | 1987-10-09 | 1993-09-14 | Monsanto Company | Modified tissue plasminogen activator with modified glycosylation site |
US5100666A (en) * | 1987-10-09 | 1992-03-31 | Monsanto Company | Modified tissue plasminogen activator K2K2SP |
US5037646A (en) * | 1988-04-29 | 1991-08-06 | Genentech, Inc. | Processes for the treatment of vascular disease |
US5346824A (en) * | 1988-05-20 | 1994-09-13 | Genentech, Inc. | DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof |
US5270198A (en) * | 1988-05-20 | 1993-12-14 | Genentech, Inc. | DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells |
US5262170A (en) * | 1988-09-02 | 1993-11-16 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells |
US5714145A (en) * | 1988-09-02 | 1998-02-03 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
PT786257E (pt) * | 1992-06-03 | 2003-12-31 | Genentech Inc | Variantes de glicosilacao do activador de plasminogenio tissular com propriedades terapeuticas meloradas |
GB9614871D0 (en) | 1996-07-15 | 1996-09-04 | Smithkline Beecham Plc | Compounds |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR241654A1 (es) * | 1982-05-05 | 1992-10-30 | Genentech Inc | Procedimiento para producir activador de plasminogeno de tejido humano. |
IE56716B1 (en) * | 1983-01-19 | 1991-11-20 | Genentech Inc | Method for producing human tpa and expression vectors therefor |
-
1986
- 1986-04-02 PT PT8232686A patent/PT82326B/pt not_active IP Right Cessation
- 1986-04-02 DK DK149786A patent/DK149786A/da not_active Application Discontinuation
- 1986-04-02 GR GR860875A patent/GR860875B/el unknown
- 1986-04-02 NZ NZ21566086A patent/NZ215660A/xx unknown
- 1986-04-02 ES ES553651A patent/ES8801374A1/es not_active Expired
- 1986-04-02 EP EP19860302425 patent/EP0207589A1/en not_active Ceased
-
1987
- 1987-08-17 ES ES557675A patent/ES8801551A1/es not_active Expired
- 1987-08-17 ES ES557674A patent/ES8802442A1/es not_active Expired
- 1987-08-17 ES ES557677A patent/ES8801552A1/es not_active Expired
- 1987-08-17 ES ES557676A patent/ES8802537A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ES553651A0 (es) | 1988-01-01 |
ES557677A0 (es) | 1987-12-16 |
ES557675A0 (es) | 1987-12-16 |
PT82326A (en) | 1986-05-01 |
AU596093B2 (en) | 1990-04-26 |
DK149786D0 (da) | 1986-04-02 |
AU5551486A (en) | 1986-10-09 |
ES557674A0 (es) | 1988-06-01 |
EP0207589A1 (en) | 1987-01-07 |
NZ215660A (en) | 1990-03-27 |
ES8801374A1 (es) | 1988-01-01 |
ES8801552A1 (es) | 1987-12-16 |
ES557676A0 (es) | 1988-07-16 |
ES8802442A1 (es) | 1988-06-01 |
ES8801551A1 (es) | 1987-12-16 |
PT82326B (pt) | 1988-10-14 |
DK149786A (da) | 1986-10-04 |
GR860875B (en) | 1986-07-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES8802442A1 (es) | Un procedimiento para preparar un polimero de adn. | |
AU5330286A (en) | Modified tissue-type plasminogen activator | |
AU4777785A (en) | Modified tissue plasminogen activators | |
ES8602118A1 (es) | Un procedimiento para preparar un conjugado de proteina fibrinoliticamente activa | |
AU7090887A (en) | Plasminogen activators modified in first kringle domain | |
GB2055850B (en) | Thermo-stable micro-organism and proteolytic enzyme prepared therefrom | |
ES360055A1 (es) | Un procedimiento para obtener preparados en enzimas proteo-liticas. | |
AU6772387A (en) | Treatment of thrombosis with fibrinolytic agents and prostacyclines | |
AU7090687A (en) | Modified tissue-type plasminogen activators | |
ES8703521A1 (es) | Procedimiento para determinar un activador de plasminogeno | |
ZA944202B (en) | Bifunctional variants of urokinase with improved fibrinolytic properties and thrombin-inhibiting activity | |
EP0351246A3 (en) | A tissue plasminogen activator analogue | |
AU2778889A (en) | Fibrin selective two-chain plasminogen activator | |
AU2196688A (en) | Modified plasminogen activators | |
EP0139447A3 (en) | Urokinase zymogen and composition containing the same | |
AU2353688A (en) | Modified tissue plasminogen activator | |
AU602681B2 (en) | Modified plasminogen activators | |
ES8704542A1 (es) | Un procedimiento para la preparacion de derivados enzimaticos. | |
IE873398L (en) | New plasminogen activators. | |
EP0292326A3 (en) | Modified enzyme | |
EP0277751A3 (en) | Modified fibrinolytic enzyme | |
WO1991019793A3 (en) | Hybrid plasminogen activators | |
BG103305A (en) | Intermediate compounds for the preparation of hiv protease inhibitors and methods for the preparation of hiv protease inhibitors | |
IE860355L (en) | Modified tissue-type plasminogen activator | |
ES8601853A1 (es) | Procedimiento para la preparacion de alfa-aminoacidos alfa-alenicos |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19970203 |