ES8801374A1 - Un procedimiento para preparar un activante de plasminogeno. - Google Patents
Un procedimiento para preparar un activante de plasminogeno.Info
- Publication number
- ES8801374A1 ES8801374A1 ES553651A ES553651A ES8801374A1 ES 8801374 A1 ES8801374 A1 ES 8801374A1 ES 553651 A ES553651 A ES 553651A ES 553651 A ES553651 A ES 553651A ES 8801374 A1 ES8801374 A1 ES 8801374A1
- Authority
- ES
- Spain
- Prior art keywords
- fibrinolytic enzyme
- modified
- region
- growth factor
- plasminogen activator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
PROCEDIMIENTO PARA LA PREPARACION DE UN ACTIVANTE DE PLASMINOGENO DE TIPO TEJIDO FIBRINOLITICAMENTE ACTIVO. COMPRENDE LAS ETAPAS DE EXPRESAR EL ADN CODIFICANTE DE LA PROTEINA EN UNA CELULA HUESPED RECOMBINANTE BAJO CONDICIONES QUE PERMITAN LA EXPRESION DEL POLIMERO DE ADN, TALES COMO EN PRESENCIA DE UN MEDIO NUTRIENTE QUE PUEDE SER UN MEDIO ACUOSO QUE CONTIENE SALES INORGANICAS Y FUENTES DE CARBONO Y NITROGENO ASIMILABLES; DE RECUPERAR EL PRODUCTO PROTEINICO MEDIANTE AISLAMIENTO DEL MISMO DEL MEDIO NUTRIENTE, SEGUIDO OPCIONALMENTE DE LISIS DE LA CELULA AFECTADA FISICAMENTE, QUIMICAMENTE O ENZIMATICAMENTE; Y DE BLOQUEAR CUALQUIER SITIO ESENCIAL PARA LA ACTIVIDAD FIBRINOLITICA CON UN GRUPO BLOQUEANTE SEPARABLE. DE APLICACION EN EL TRATAMIENTO DE ENFERMEDADES TROMBOTICAS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB858508972A GB8508972D0 (en) | 1985-04-04 | 1985-04-04 | Compounds |
GB868604852A GB8604852D0 (en) | 1986-02-27 | 1986-02-27 | Compounds |
Publications (2)
Publication Number | Publication Date |
---|---|
ES553651A0 ES553651A0 (es) | 1988-01-01 |
ES8801374A1 true ES8801374A1 (es) | 1988-01-01 |
Family
ID=26289098
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES553651A Expired ES8801374A1 (es) | 1985-04-04 | 1986-04-02 | Un procedimiento para preparar un activante de plasminogeno. |
ES557677A Expired ES8801552A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa. |
ES557675A Expired ES8801551A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un vector de expresion replicable. |
ES557676A Expired ES8802537A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado. |
ES557674A Expired ES8802442A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un polimero de adn. |
Family Applications After (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES557677A Expired ES8801552A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una proteina hibrida fibrinoliticamente activa. |
ES557675A Expired ES8801551A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un vector de expresion replicable. |
ES557676A Expired ES8802537A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar una celula huesped con un vector de expresion correspondiente a un activante de plasminogenos de tipo tejido modificado. |
ES557674A Expired ES8802442A1 (es) | 1985-04-04 | 1987-08-17 | Un procedimiento para preparar un polimero de adn. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0207589A1 (es) |
DK (1) | DK149786A (es) |
ES (5) | ES8801374A1 (es) |
GR (1) | GR860875B (es) |
NZ (1) | NZ215660A (es) |
PT (1) | PT82326B (es) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR880700856A (ko) | 1985-12-20 | 1988-04-12 | 로버어트 에이 아미테이지 | 조직 플라스미노겐 활성화인자(tpa)상사체 |
US5106741A (en) * | 1985-12-20 | 1992-04-21 | The Upjohn Company | Tissue plasminogen activator (TPA) analogs |
US5258298A (en) * | 1986-01-31 | 1993-11-02 | Genetics Institute, Inc. | Deletion and glycosylation mutant of human tissue plasminogen activator |
US5837518A (en) * | 1986-01-31 | 1998-11-17 | Genetics Institute, Inc. | Thrombolytic proteins |
US5002887A (en) * | 1986-01-31 | 1991-03-26 | Genetics Institute, Inc. | Truncated thrombolytic proteins |
ATE70301T1 (de) * | 1986-02-17 | 1991-12-15 | Stichting Centraal Lab | Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen. |
PT84588B (en) * | 1986-04-02 | 1989-05-09 | Beecham Group Plc | Process for preparing a fibrinolytic enzyme |
PT84589B (en) * | 1986-04-02 | 1989-05-09 | Beecham Group Plc | Process for preparing a fibrinolytic enzyme |
PT84587B (pt) * | 1986-04-02 | 1989-11-30 | Beecham Group Plc | Processo para preparacao de uma enzima fibrinolitica |
DE3643158A1 (de) * | 1986-04-21 | 1987-11-19 | Boehringer Mannheim Gmbh | Gewebs-plasminogen-aktivator(tpa) - derivat und seine herstellung |
EP0266032A1 (en) * | 1986-08-29 | 1988-05-04 | Beecham Group Plc | Modified fibrinolytic enzyme |
US5580559A (en) * | 1986-12-05 | 1996-12-03 | Ciba-Geigy Corporation | Hybrid plasminogen activator |
US5242819A (en) * | 1986-12-05 | 1993-09-07 | Ciba-Geigy Corporation | DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase |
GB8701811D0 (en) * | 1987-01-28 | 1987-03-04 | Beecham Group Plc | Compounds |
US5376547A (en) * | 1987-01-30 | 1994-12-27 | American Home Products Corporation | Des-epidermal growth factor plasminogen activators |
NL8701021A (nl) * | 1987-04-29 | 1988-11-16 | Stichting Centraal Lab | Humane t-pa(u-pa) substitutie-mutant eiwitten, daarvoor coderend recombinant dna, getransfecteerde gastheercellen, bereiding van de mutant eiwitten, en farmaceutische preparaten. |
IE67036B1 (en) * | 1987-06-04 | 1996-05-15 | Zymogenetics Inc | Tissue plasminogen activator analogs having modified growth factor domains |
ATE110772T1 (de) * | 1987-06-04 | 1994-09-15 | Zymogenetics Inc | Mutierter t-pa mit kringle-ersatz. |
SE8702562L (sv) * | 1987-06-18 | 1988-12-19 | Kabigen Ab | Nya fibrinolytiska enzymer |
DE3886755T3 (de) * | 1987-06-30 | 2005-12-08 | Genentech, Inc., South San Francisco | Verfahren zur behandlung von gefässkrankheiten. |
AU613942B2 (en) * | 1987-06-30 | 1991-08-15 | Genentech Inc. | Improved processes for the treatment of vascular disease |
US4935237A (en) * | 1988-03-21 | 1990-06-19 | Genentech, Inc. | Processes for the preparation of t-PA mutants |
WO1989000191A1 (en) * | 1987-07-06 | 1989-01-12 | Genetics Institute, Inc. | Novel thrombolytic proteins |
GB8721023D0 (en) * | 1987-09-07 | 1987-10-14 | Lepetit Spa | Modified plasminogen activators |
US5244676A (en) * | 1987-10-09 | 1993-09-14 | Monsanto Company | Modified tissue plasminogen activator with modified glycosylation site |
US5100666A (en) * | 1987-10-09 | 1992-03-31 | Monsanto Company | Modified tissue plasminogen activator K2K2SP |
US5037646A (en) * | 1988-04-29 | 1991-08-06 | Genentech, Inc. | Processes for the treatment of vascular disease |
US5346824A (en) * | 1988-05-20 | 1994-09-13 | Genentech, Inc. | DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof |
US5270198A (en) * | 1988-05-20 | 1993-12-14 | Genentech, Inc. | DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells |
US5714145A (en) * | 1988-09-02 | 1998-02-03 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
US5262170A (en) * | 1988-09-02 | 1993-11-16 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells |
AU664469B2 (en) * | 1992-06-03 | 1995-11-16 | Genentech Inc. | Tissue plasminogen activator glycosylation variants with improved therapeutic properties |
GB9614871D0 (en) | 1996-07-15 | 1996-09-04 | Smithkline Beecham Plc | Compounds |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL68561A (en) * | 1982-05-05 | 1991-01-31 | Genentech Inc | Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof |
AU572108B2 (en) * | 1983-01-19 | 1988-05-05 | Genentech Inc. | Human tpa production using vectors coding for dhfr protein |
-
1986
- 1986-04-02 PT PT8232686A patent/PT82326B/pt not_active IP Right Cessation
- 1986-04-02 GR GR860875A patent/GR860875B/el unknown
- 1986-04-02 NZ NZ21566086A patent/NZ215660A/xx unknown
- 1986-04-02 DK DK149786A patent/DK149786A/da not_active Application Discontinuation
- 1986-04-02 EP EP19860302425 patent/EP0207589A1/en not_active Ceased
- 1986-04-02 ES ES553651A patent/ES8801374A1/es not_active Expired
-
1987
- 1987-08-17 ES ES557677A patent/ES8801552A1/es not_active Expired
- 1987-08-17 ES ES557675A patent/ES8801551A1/es not_active Expired
- 1987-08-17 ES ES557676A patent/ES8802537A1/es not_active Expired
- 1987-08-17 ES ES557674A patent/ES8802442A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
GR860875B (en) | 1986-07-29 |
ES8802537A1 (es) | 1988-07-16 |
ES557675A0 (es) | 1987-12-16 |
ES553651A0 (es) | 1988-01-01 |
DK149786A (da) | 1986-10-04 |
EP0207589A1 (en) | 1987-01-07 |
PT82326B (pt) | 1988-10-14 |
ES557677A0 (es) | 1987-12-16 |
ES8802442A1 (es) | 1988-06-01 |
ES557674A0 (es) | 1988-06-01 |
DK149786D0 (da) | 1986-04-02 |
NZ215660A (en) | 1990-03-27 |
AU596093B2 (en) | 1990-04-26 |
ES8801551A1 (es) | 1987-12-16 |
ES8801552A1 (es) | 1987-12-16 |
ES557676A0 (es) | 1988-07-16 |
AU5551486A (en) | 1986-10-09 |
PT82326A (en) | 1986-05-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19970303 |