ES8707563A1 - Un procedimiento para purificar urocinasa - Google Patents

Un procedimiento para purificar urocinasa

Info

Publication number
ES8707563A1
ES8707563A1 ES552073A ES552073A ES8707563A1 ES 8707563 A1 ES8707563 A1 ES 8707563A1 ES 552073 A ES552073 A ES 552073A ES 552073 A ES552073 A ES 552073A ES 8707563 A1 ES8707563 A1 ES 8707563A1
Authority
ES
Spain
Prior art keywords
urokinase
immono
immunoassays
epitope
impair
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES552073A
Other languages
English (en)
Other versions
ES552073A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gruppo Lepetit SpA
Original Assignee
Gruppo Lepetit SpA
Lepetit SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB848422007A external-priority patent/GB8422007D0/en
Priority claimed from GB858508244A external-priority patent/GB8508244D0/en
Priority claimed from GB858510716A external-priority patent/GB8510716D0/en
Application filed by Gruppo Lepetit SpA, Lepetit SpA filed Critical Gruppo Lepetit SpA
Publication of ES8707563A1 publication Critical patent/ES8707563A1/es
Publication of ES552073A0 publication Critical patent/ES552073A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/972—Plasminogen activators
    • G01N2333/9723—Urokinase

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCEIDMIENTO PARA LA PURIFICACION DE UROCINASA. COMPRENDE LAS OPERACIONES DE PONER EN CONTACTO, A UNA TEMPERATURA COMPRENDIDA ENTRE LA AMBIENTE Y APROXIMADAMENTE 60 GRADOS, UNA FUENTE BIOLOGICA O UN CONCENTRADO DE LA MISMA, CON UN INMUNOADSORBENTE CONSTITUIDO POR UN ANTICUERPO ANTI-UROCINASA; DE LAVAR EL INMUNOADSORBENTE CON UNA DISOLUCION TAMPONADA A UN PH COMPRENDIDO ENTRE 6 Y 8; Y DE DESPRENDER EL ANTIGENO LIGADO DEL INMUNOADSORBENTE POR ELUCION CON UNA DISOLUCION ELUYENTE QUE CONTIENE OPCIONALMENTE CLORURO DE SODIO ACUOSO, A UN PH COMPRENDIDO ENTRE 3 Y 4. DE APLICACION EN UN SISTEMA DE PURIFICACION BASADO EN INMUNOADSORCION.
ES552073A 1984-08-31 1986-02-17 Un procedimiento para purificar urocinasa Expired ES8707563A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB848422007A GB8422007D0 (en) 1984-08-31 1984-08-31 Anti-urokinase monoclonal antibody
GB858508244A GB8508244D0 (en) 1985-03-29 1985-03-29 Anti-urokinase monoclonal antibody
GB858510716A GB8510716D0 (en) 1985-04-26 1985-04-26 Anti-urokinase monoclonal antibody

Publications (2)

Publication Number Publication Date
ES8707563A1 true ES8707563A1 (es) 1987-08-01
ES552073A0 ES552073A0 (es) 1987-08-01

Family

ID=27262453

Family Applications (3)

Application Number Title Priority Date Filing Date
ES546594A Expired ES8704351A1 (es) 1984-08-31 1985-08-30 Un procedimiento para preparar un anticuerpo monocional anti-urocinasa.
ES552073A Expired ES8707563A1 (es) 1984-08-31 1986-02-17 Un procedimiento para purificar urocinasa
ES552074A Expired ES8706445A1 (es) 1984-08-31 1986-02-17 Un metodo en emparedado de doble anticuerpo para detectar o ensayar un antigeno seleccionado de urocinasa humana y un precursor de la misma

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES546594A Expired ES8704351A1 (es) 1984-08-31 1985-08-30 Un procedimiento para preparar un anticuerpo monocional anti-urocinasa.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES552074A Expired ES8706445A1 (es) 1984-08-31 1986-02-17 Un metodo en emparedado de doble anticuerpo para detectar o ensayar un antigeno seleccionado de urocinasa humana y un precursor de la misma

Country Status (16)

Country Link
EP (1) EP0192675B1 (es)
KR (1) KR930002834B1 (es)
AT (1) ATE56617T1 (es)
AU (1) AU590216B2 (es)
CA (1) CA1297818C (es)
DE (1) DE3579808D1 (es)
DK (1) DK176986A (es)
ES (3) ES8704351A1 (es)
FI (1) FI86255C (es)
GR (1) GR852008B (es)
HU (1) HU203257B (es)
IL (1) IL76187A0 (es)
NO (1) NO172549C (es)
NZ (1) NZ213125A (es)
PT (1) PT81050B (es)
WO (1) WO1986001411A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8604851D0 (en) * 1986-02-27 1986-04-03 Lepetit Spa Immunosorbent-amidolytic method
JP2635343B2 (ja) * 1986-04-14 1997-07-30 ザ・ジェネラル・ホスピタル・コーポレーション ヘテロ二官能性抗体および利用方法
AT388618B (de) * 1987-05-05 1989-08-10 Binder Bernd Dr Verfahren zur quantitativen bestimmung von funktion und antigener konzentration einer in einer biologischen fluessigkeit enthaltenen substanz
US5811265A (en) * 1988-08-19 1998-09-22 The General Hospital Corporation Hybrid immunoglobulin-thrombolytic enzyme molecules which specifically bind a thrombus, and methods of their production and use
US5609869A (en) * 1988-08-19 1997-03-11 The General Hospital Corporation Hybrid immunoglobulin-thrombolytic enzyme molecules which specifically bind a thrombus, and methods of their production and use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3790663A (en) * 1970-07-07 1974-02-05 Us Health Preparation of dry antiserum coated solid-phase for radioimmunoassay of antigens
EP0084344A3 (en) * 1982-01-14 1983-08-03 Asahi Kasei Kogyo Kabushiki Kaisha Anti-urokinase monoclonal antibodies and process for preparing the same
BE896253A (fr) * 1983-03-24 1983-07-18 Wallone Region Anticorps monoclonaux diriges contre l'urokinase humaine
WO1985000663A1 (en) * 1983-07-25 1985-02-14 Beckman Instruments, Inc. Immunometric assay using polyclonal and monoclonal antibodies and a kit for use therein

Also Published As

Publication number Publication date
HU203257B (en) 1991-06-28
ES552074A0 (es) 1987-07-01
FI861715A0 (fi) 1986-04-24
ATE56617T1 (de) 1990-10-15
HUT40574A (en) 1987-01-28
DE3579808D1 (de) 1990-10-25
KR870700022A (ko) 1987-02-28
DK176986D0 (da) 1986-04-17
AU4773085A (en) 1986-03-24
NO172549C (no) 1993-08-04
ES546594A0 (es) 1987-04-01
EP0192675B1 (en) 1990-09-19
PT81050B (pt) 1987-10-20
ES8704351A1 (es) 1987-04-01
GR852008B (es) 1985-12-30
NO861554L (no) 1986-04-18
KR930002834B1 (ko) 1993-04-10
FI861715A (fi) 1986-04-24
PT81050A (en) 1985-09-01
IL76187A0 (en) 1985-12-31
ES552073A0 (es) 1987-08-01
NZ213125A (en) 1991-06-25
CA1297818C (en) 1992-03-24
AU590216B2 (en) 1989-11-02
ES8706445A1 (es) 1987-07-01
FI86255C (fi) 1992-08-10
DK176986A (da) 1986-04-17
FI86255B (fi) 1992-04-30
NO172549B (no) 1993-04-26
EP0192675A1 (en) 1986-09-03
WO1986001411A1 (en) 1986-03-13

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970505