ES8605578A1 - Un procedimiento para producir un adn complementario clonadoo su adn modificado que codifica un polipeptido de factor denecrosis de tumores - Google Patents

Un procedimiento para producir un adn complementario clonadoo su adn modificado que codifica un polipeptido de factor denecrosis de tumores

Info

Publication number
ES8605578A1
ES8605578A1 ES547436A ES547436A ES8605578A1 ES 8605578 A1 ES8605578 A1 ES 8605578A1 ES 547436 A ES547436 A ES 547436A ES 547436 A ES547436 A ES 547436A ES 8605578 A1 ES8605578 A1 ES 8605578A1
Authority
ES
Spain
Prior art keywords
vector
rabbit tnf
dna
polypeptide
inserted thereinto
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES547436A
Other languages
English (en)
Other versions
ES547436A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dainippon Pharmaceutical Co Ltd
Original Assignee
Dainippon Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dainippon Pharmaceutical Co Ltd filed Critical Dainippon Pharmaceutical Co Ltd
Publication of ES547436A0 publication Critical patent/ES547436A0/es
Publication of ES8605578A1 publication Critical patent/ES8605578A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA PRODUCIR UN ADN COMPLEMENTARIO CLONADO O SU ADN MODIFICADO QUE CODIFICA UN POLIPEPTIDO DE FACTOR DE NECROSIS DE TUMORES. COMPRENDE: A) CULTIVAR MACROFAGOS DE CONEJO CON INDUCTORES; B) SEPARAR UNA FRACCION CONTENIENDO UN ARN MENSAJERO (ARNM) DE FACTOR DE NECROSIS DE TUMORES DE CONEJO DEL CULTIVO DE LOS MACROFAGOS; C) TRATAR AL ARNM CON TRANSCRIPTASA INVERSA, PARA REALIZAR UN ADN COMPLEMENTARIO (ADNC) MONOCATENARIO; D) CONVERTIR AL ADNC MONOCATENARIO EN UN ADNC DE DOBLE CADENA; E) INSERTAR EL ADNC DE DOBLE CADENA EN UN VECTOR DE PLASMIDO; F) INTRODUCIR A LOS PLASMIDOS RECOMBINANTES EN UN HOSPEDANTE PARA TRANSFORMAR AL ADNC DE DOBLE CADENA Y CONSTRUIR UNA BIBLIOTECA DE ADNC; Y G HIBRIDAR A LA BIBLIOTECA DE ADNC PARA OBTENER ADNC QUE CODIFICA EL FACTOR DE NECROSIS DE TUMORES DE CONEJO.
ES547436A 1983-12-02 1985-09-30 Un procedimiento para producir un adn complementario clonadoo su adn modificado que codifica un polipeptido de factor denecrosis de tumores Expired ES8605578A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58228790A JPH0695939B2 (ja) 1983-12-02 1983-12-02 ウサギ癌壊死因子をコ−ドするクロ−ン化ｄνａ

Publications (2)

Publication Number Publication Date
ES547436A0 ES547436A0 (es) 1986-03-16
ES8605578A1 true ES8605578A1 (es) 1986-03-16

Family

ID=16881888

Family Applications (2)

Application Number Title Priority Date Filing Date
ES538000A Expired ES8604309A1 (es) 1983-12-02 1984-11-27 Un procedimiento para obtener un adn que codifica un factor de necrosis de tumor de conejo
ES547436A Expired ES8605578A1 (es) 1983-12-02 1985-09-30 Un procedimiento para producir un adn complementario clonadoo su adn modificado que codifica un polipeptido de factor denecrosis de tumores

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES538000A Expired ES8604309A1 (es) 1983-12-02 1984-11-27 Un procedimiento para obtener un adn que codifica un factor de necrosis de tumor de conejo

Country Status (10)

Country Link
US (1) US5043271A (es)
EP (1) EP0146026B1 (es)
JP (1) JPH0695939B2 (es)
KR (1) KR920009523B1 (es)
AT (1) ATE60933T1 (es)
AU (1) AU577810B2 (es)
DE (1) DE3484125D1 (es)
DK (1) DK572284A (es)
ES (2) ES8604309A1 (es)
PH (1) PH26947A (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1265444A (en) * 1983-06-27 1990-02-06 Lloyd J. Old Effect of human tumor necrosis factor and human interferon on human cancer cells and methods
JPS60112718A (ja) * 1983-11-21 1985-06-19 Kyorin Pharmaceut Co Ltd 抗腫瘍作用を示す蛋白性物質及びその製造方法
JPS60139624A (ja) * 1983-12-27 1985-07-24 Dainippon Pharmaceut Co Ltd 抗腫瘍作用を有する蛋白質
US4879226A (en) * 1984-04-06 1989-11-07 Asahi Kasei Kogyo Kabushiki Kaisha Novel human physiologically active polypeptide
GR851626B (es) * 1984-07-05 1985-11-26 Genentech Inc
US6686455B1 (en) 1984-07-05 2004-02-03 Genentech, Inc. Tumor necrosis factor
US5672347A (en) * 1984-07-05 1997-09-30 Genentech, Inc. Tumor necrosis factor antagonists and their use
JP2557053B2 (ja) * 1984-12-21 1996-11-27 バイオジェン インコーポレイテッド 腫瘍壊死因子の精製、製造および使用法
US5425940A (en) * 1986-04-09 1995-06-20 Cetus Oncology Corporation Combination therapy using interleukin-2 and tumor necrosis factor
US5998378A (en) * 1989-08-16 1999-12-07 Chiron Corporation Compositions for the inhibition of TNF hormone formation and uses thereof
DK0491878T3 (da) 1989-08-16 1997-06-23 Chiron Corp Præparater til inhibering af proteinhormondannelse og anvendelser deraf
US6586222B1 (en) 1989-08-16 2003-07-01 Chiron Corporation Recombinant PR-3 and compositions thereof
US5843693A (en) * 1989-08-16 1998-12-01 Chiron Corporation Assay method for screening for inhibitors of proTNF conversion
WO1991017180A1 (en) * 1990-04-27 1991-11-14 Peptide Technology Ltd Peptides derived from human tumour necrosis factor alpha and useful against intracellular malarial parasites
US5653974A (en) * 1990-10-18 1997-08-05 Board Of Regents,The University Of Texas System Preparation and characterization of liposomal formulations of tumor necrosis factor
WO1995024501A1 (en) 1994-03-07 1995-09-14 Cetus Oncology Corporation Compositions for the inhibition of tnf formation and uses thereof
ES2347239T3 (es) 2002-12-02 2010-10-27 Amgen Fremont Inc. Anticuerpos dirigidos al factor de necrosis tumoral y usos de los mismos.

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57140725A (en) * 1981-12-28 1982-08-31 Dainippon Pharmaceut Co Ltd Physiologically active substance having carcinostatic action
JPS6019719A (ja) * 1983-07-15 1985-01-31 Asahi Chem Ind Co Ltd 抗腫瘍活性を有する蛋白質
DE3472793D1 (en) * 1983-12-26 1988-08-25 Asahi Chemical Ind A novel physiologically active polypeptide
EP0155549B1 (en) * 1984-03-06 1991-03-20 Dainippon Pharmaceutical Co., Ltd. Dna encoding human tumor necrosis factor and human tumor necrosis factor polypeptide
US4879226A (en) * 1984-04-06 1989-11-07 Asahi Kasei Kogyo Kabushiki Kaisha Novel human physiologically active polypeptide

Also Published As

Publication number Publication date
EP0146026A3 (en) 1987-05-20
DK572284A (da) 1985-06-03
ES8604309A1 (es) 1986-01-16
JPS60120990A (ja) 1985-06-28
ATE60933T1 (de) 1991-03-15
DK572284D0 (da) 1984-11-30
EP0146026A2 (en) 1985-06-26
AU3601484A (en) 1985-06-06
KR920009523B1 (ko) 1992-10-17
AU577810B2 (en) 1988-10-06
ES547436A0 (es) 1986-03-16
KR850004272A (ko) 1985-07-11
PH26947A (en) 1992-12-03
EP0146026B1 (en) 1991-02-20
ES538000A0 (es) 1986-01-16
DE3484125D1 (de) 1991-03-28
US5043271A (en) 1991-08-27
JPH0695939B2 (ja) 1994-11-30

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Legal Events

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FD1A Patent lapsed

Effective date: 20010402