ES8605104A1 - Procedimiento para la determinacion cuantitativa inmunoqui- mica de una sustancia activa como antigeno - Google Patents

Procedimiento para la determinacion cuantitativa inmunoqui- mica de una sustancia activa como antigeno

Info

Publication number
ES8605104A1
ES8605104A1 ES546794A ES546794A ES8605104A1 ES 8605104 A1 ES8605104 A1 ES 8605104A1 ES 546794 A ES546794 A ES 546794A ES 546794 A ES546794 A ES 546794A ES 8605104 A1 ES8605104 A1 ES 8605104A1
Authority
ES
Spain
Prior art keywords
phase
solid
liq
haptens
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546794A
Other languages
English (en)
Other versions
ES546794A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES8605104A1 publication Critical patent/ES8605104A1/es
Publication of ES546794A0 publication Critical patent/ES546794A0/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G01N33/541—Double or second antibody, i.e. precipitating antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9453—Cardioregulators, e.g. antihypotensives, antiarrhythmics
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/971—Capture of complex after antigen-antibody reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Rehabilitation Therapy (AREA)
  • Rheumatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA DETERMINACION CUANTITATIVA INMUNOQUIMICA DE UNA SUSTANCIA ACTIVA COMO ANTIGENO. COMPRENDE: A) INCUBAR DE LA SUSTANCIA A DETERMINAR CON UN FIJADOR, PARA FORMAR UNA MEZCLA; B) INCUBAR A LA MEZCLA DE REACCION CON SUSTANCIA A DETERMINAR EN FASE SOLIDA O CON UN ANALOGO DE LA MISMA, PARA FORMAR UNA FASE SOLIDA EN SOLUCION; C) SEPARAR LA FASE SOLIDA DE LA FASE LIQUIDA (SOLUCION); D) LAVAR A LA FASE SOLIDA; E) AÑADIR LIQUIDO DE LAVADO A LA FASE LIQUIDA; F) PONER EN CONTACTO A LA FASE LIQUIDA CON UN SEGUNDO ANTICUERPO EN FASE SOLIDA; G) SEPARAR LA FASE SOLIDA DE LA SOLUCION; Y H) MEDIR LA MARCACION FIJADA DE FASE SOLIDA. EL FIJADOR CONSTA DE UN PRIMER ANTICUERPO MARCADO O DE UN FRAGMENTO FAB O FAB. LA SUSTANCIA ACTIVA ES UN HAPTENO, EL MARCADOR ES UNA ENZIMA, COMO PEROXIDASA O FOSFATASA ALCALINA, Y EL SEGUNDO ANTICUERPO SE DIRIGE CONTRA EL FIJADOR O CONTRA EL COMPLEJO DE FIJADOR A BASE DE FIJADOR Y SUSTANCIA A DETERMINAR Y ESTA FORMADO POR MONOCLONAL O UN FRAGMENTO FAB/FAB DELMISMO.
ES546794A 1984-09-13 1985-09-09 Procedimiento para la determinacion cuantitativa inmunoqui- mica de una sustancia activa como antigeno Expired ES8605104A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843433652 DE3433652A1 (de) 1984-09-13 1984-09-13 Immunchemisches messverfahren fuer haptene und proteine

Publications (2)

Publication Number Publication Date
ES8605104A1 true ES8605104A1 (es) 1986-03-01
ES546794A0 ES546794A0 (es) 1986-03-01

Family

ID=6245328

Family Applications (1)

Application Number Title Priority Date Filing Date
ES546794A Expired ES8605104A1 (es) 1984-09-13 1985-09-09 Procedimiento para la determinacion cuantitativa inmunoqui- mica de una sustancia activa como antigeno

Country Status (7)

Country Link
US (1) US4670383A (es)
EP (1) EP0174652B1 (es)
JP (1) JPS6171361A (es)
AT (1) ATE51449T1 (es)
CA (1) CA1256025A (es)
DE (2) DE3433652A1 (es)
ES (1) ES8605104A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8408193D0 (en) * 1984-03-30 1984-05-10 Cambridge Patent Dev Antibodies
GB2161165A (en) * 1984-06-12 1986-01-08 Cambridge Patent Dev Secondary antibodies
AU7485987A (en) * 1986-05-28 1987-12-22 Murex Corp. Method for producing monoclonal antibodies to an immunogenic antibody-antigen conjunction
US5223441A (en) 1986-10-09 1993-06-29 Syntex (U.S.A.) Inc. Receptors for immune complexes
DE3705686C2 (de) * 1987-02-23 1995-11-30 Boehringer Mannheim Gmbh Verfahren zur Bestimmung von Antikörpern
US5236849A (en) * 1987-08-11 1993-08-17 Eiji Ishikawa Method of high sensitivity immunoassay
DE3852797T2 (de) * 1987-08-11 1995-06-29 Eiji Ishikawa Hoch empfindlicher Immunoassay.
US5468651A (en) * 1987-11-28 1995-11-21 Cambridge Patent Developments Limited Method for determining haptens, use of method and components useful in method
US6287793B1 (en) 1988-08-19 2001-09-11 Elan Pharmaceuticals, Inc. Diagnostic methods for alzheimer's disease
US5445936A (en) * 1993-09-15 1995-08-29 Ciba Corning Diagnostics Corp. Method for non-competitive binding assays
DE4418513A1 (de) * 1994-05-27 1995-11-30 Bayer Ag Immuntest zum Nachweis hochmolekularer Antigene
US5817527A (en) * 1995-11-06 1998-10-06 Chiron Diagnostics Corporation Conjugation of ligand to immobilized protein in organic solvent
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
JPH11153599A (ja) * 1997-11-21 1999-06-08 Dai Ichi Pure Chem Co Ltd 免疫学的測定方法
WO2003015871A2 (en) * 2001-07-25 2003-02-27 University Of North Carolina At Chapel Hill Method and apparatus for rapid determination of ligand-protein binding using charcoal adsorption
US7341837B2 (en) 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
JP2009508695A (ja) * 2005-09-16 2009-03-05 パスクワーレ カタルファーモ， 研磨体
FI20085579A0 (fi) * 2008-06-12 2008-06-12 Valtion Teknillinen Kannabiskäytön toteaminen
DE102008049601A1 (de) * 2008-09-30 2010-04-01 Siemens Healthcare Diagnostics Products Gmbh Antikörper zur Bestimmung des Prothrombin-Fragments F2/F1+2 in einem homogenen Immunoassay
CN111138381B (zh) * 2019-12-25 2022-05-31 国家海洋环境监测中心 一种烯酰吗啉半抗原及其制备方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3720760A (en) * 1968-09-06 1973-03-13 Pharmacia Ab Method for determining the presence of reagin-immunoglobulins(reagin-ig)directed against certain allergens,in aqueous samples
NL154598B (nl) * 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US4048298A (en) * 1975-02-25 1977-09-13 Rohm And Haas Company Solid phase double-antibody radioimmunoassay procedure
US4056608A (en) * 1976-04-08 1977-11-01 Syva Company Cardiac glycoside or aglycone assays
JPS5344622A (en) * 1976-09-30 1978-04-21 Mochida Pharm Co Ltd Immunologically measuring method

Also Published As

Publication number Publication date
EP0174652A3 (en) 1987-07-01
CA1256025A (en) 1989-06-20
EP0174652A2 (de) 1986-03-19
ATE51449T1 (de) 1990-04-15
JPS6171361A (ja) 1986-04-12
US4670383A (en) 1987-06-02
EP0174652B1 (de) 1990-03-28
JPH0467914B2 (es) 1992-10-29
ES546794A0 (es) 1986-03-01
DE3433652A1 (de) 1986-03-20
DE3576854D1 (de) 1990-05-03

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