ES8602117A1 - Un procedimiento para producir un uroquinasa-zimogeno - Google Patents

Un procedimiento para producir un uroquinasa-zimogeno

Info

Publication number
ES8602117A1
ES8602117A1 ES535847A ES535847A ES8602117A1 ES 8602117 A1 ES8602117 A1 ES 8602117A1 ES 535847 A ES535847 A ES 535847A ES 535847 A ES535847 A ES 535847A ES 8602117 A1 ES8602117 A1 ES 8602117A1
Authority
ES
Spain
Prior art keywords
zymogen
plasminogen activator
human
culturing
activator activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES535847A
Other languages
English (en)
Other versions
ES535847A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP58170354A external-priority patent/JPH0736755B2/ja
Priority claimed from JP58195051A external-priority patent/JPS6087226A/ja
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Publication of ES535847A0 publication Critical patent/ES535847A0/es
Publication of ES8602117A1 publication Critical patent/ES8602117A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PROCEDIMIENTO PARA PRODUCIR UN ZIMOGENO DE UROQUINASA. CONSISTE EN CULTIVAR CELULAS DE RIÑON HUMANO EN UN MEDIO DE CULTIVO Y RECUPERARLO DEL FLUIDO DE CULTIVO POR CROMATOGRAFIA DE AFINIDAD EMPLEANDO UNA COLUMNA DE ANTICUERPO INMOVILIZADO ANTI-ZIMOGENO; DONDE EL ZIMOGENO ES UN POLIPEPTIDO QUE TIENE UNA UNICA CADENA CON UN PESO MOLECULAR DE 50.000, Y AUNQUE EL ZIMOGENO POR SI MISMO PRESENTA POCA ACTIVIDAD DE ACTIVADOR PLASMINOGENO, MANIFIESTA UNA ACTIVIDAD DE ACTIVADOR PLASMINOGENO SIGNIFICATIVA POR CANTIDADES CATALITICAS DE PLASMINA; Y DONDE EL ZIMOGENO TIENE UNA CAPACIDAD TROMBOLITICA TAN ELEVADA COMO TRES VECES LA DE LA UROQUINASA DE LA ORINA HUMANA, PUDIENDO ESTABILIZARSE POR ADICION DE ALBUMINA Y POR CONSIGUIENTE PUDIENDO CONSEGUIRSE SUS PREPARACIONES LIOFILIZADAS ESTABLES. SE UTILIZA FARMACEUTICAMENTE PARA EL TRATAMIENTO DE LOS TROMBOS HUMANOS.
ES535847A 1983-09-13 1984-09-12 Un procedimiento para producir un uroquinasa-zimogeno Expired ES8602117A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP58170354A JPH0736755B2 (ja) 1983-09-13 1983-09-13 プラスミノーゲン・アクチベーター前駆体含有組成物
JP58195051A JPS6087226A (ja) 1983-10-17 1983-10-17 プラスミノーゲン・アクチベーター前駆体組成物

Publications (2)

Publication Number Publication Date
ES535847A0 ES535847A0 (es) 1985-11-01
ES8602117A1 true ES8602117A1 (es) 1985-11-01

Family

ID=26493364

Family Applications (2)

Application Number Title Priority Date Filing Date
ES535847A Expired ES8602117A1 (es) 1983-09-13 1984-09-12 Un procedimiento para producir un uroquinasa-zimogeno
ES543737A Granted ES543737A0 (es) 1983-09-13 1985-05-31 Un metodo de estabilizar el zimogeno de uroquinasa

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES543737A Granted ES543737A0 (es) 1983-09-13 1985-05-31 Un metodo de estabilizar el zimogeno de uroquinasa

Country Status (4)

Country Link
EP (1) EP0139447B1 (es)
CA (1) CA1258242A (es)
DE (1) DE3486023T2 (es)
ES (2) ES8602117A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61176532A (ja) * 1985-01-30 1986-08-08 Green Cross Corp:The プラスミノ−ゲンアクチベ−タ−前駆体の安定化方法
KR940003056B1 (ko) * 1985-04-16 1994-04-13 가부시끼가이샤 미도리 쥬우지 우로키나제 전구체를 안정화시키는 방법
JPS62149625A (ja) * 1985-12-25 1987-07-03 Green Cross Corp:The 生理活性物質の製造方法
NL8601240A (nl) * 1986-05-15 1987-12-01 Leuven Res & Dev Vzw Plasminogeenactivator en trombolytisch geneesmiddel.
JPH0761266B2 (ja) * 1986-07-03 1995-07-05 株式会社ミドリ十字 変異ヒトプロウロキナ−ゼ
JP2764264B2 (ja) * 1987-10-01 1998-06-11 株式会社ミドリ十字 線溶活性増強剤
EP2126067B1 (en) * 2007-02-23 2014-07-02 Landing Biotech, Inc. Treating atherosclerosis
CN106929497B (zh) * 2017-05-05 2018-04-17 江苏尤里卡生物科技有限公司 一种尿激酶的分离纯化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6050436B2 (ja) * 1978-06-17 1985-11-08 武彦 山本 ウロキナ−ゼの製造法
AU6642281A (en) * 1979-11-13 1981-06-03 Husain, S.S. Isolation of plasminogen activators useful as therapeutic anddiagnostic agents
FI88932C (fi) * 1982-04-15 1993-07-26 Genentech Inc Framstaellning av funktionellt maenskligt urokinasprotein
BE896253A (fr) * 1983-03-24 1983-07-18 Wallone Region Anticorps monoclonaux diriges contre l'urokinase humaine

Also Published As

Publication number Publication date
DE3486023D1 (de) 1993-02-11
ES535847A0 (es) 1985-11-01
EP0139447B1 (en) 1992-12-30
CA1258242A (en) 1989-08-08
EP0139447A2 (en) 1985-05-02
ES8603951A1 (es) 1986-01-01
ES543737A0 (es) 1986-01-01
EP0139447A3 (en) 1985-06-05
DE3486023T2 (de) 1993-06-24

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Legal Events

Date Code Title Description
PC1A Transfer granted