ES8600059A1 - Procedimiento para la preparacion de un plasmido recombinante insertado con el gen del virus de la hepatitis b. - Google Patents
Procedimiento para la preparacion de un plasmido recombinante insertado con el gen del virus de la hepatitis b.Info
- Publication number
- ES8600059A1 ES8600059A1 ES83524942A ES524942A ES8600059A1 ES 8600059 A1 ES8600059 A1 ES 8600059A1 ES 83524942 A ES83524942 A ES 83524942A ES 524942 A ES524942 A ES 524942A ES 8600059 A1 ES8600059 A1 ES 8600059A1
- Authority
- ES
- Spain
- Prior art keywords
- hepatitis
- virus
- recombinant plasmid
- gene
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000700721 Hepatitis B virus Species 0.000 title abstract 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 239000000427 antigen Substances 0.000 title abstract 4
- 108091007433 antigens Proteins 0.000 title abstract 4
- 102000036639 antigens Human genes 0.000 title abstract 4
- 239000013612 plasmid Substances 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 229940124872 Hepatitis B virus vaccine Drugs 0.000 abstract 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 abstract 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 abstract 1
- 101710167911 Repressible acid phosphatase Proteins 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000001900 immune effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 abstract 1
- 239000013600 plasmid vector Substances 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Abstract
PROCEDIMIENTO PARA LA PREPARACION DE UN PLASMIDO RECOMBINANTE INSERTADO CON EL GEN DEL VIRUS DE LA HEPATITIS B.COMPRENDE: RECOMBINAR UN GEN DE ANTIGENO DE HBS EN UN VECTOR REVERSIBLE EN EL SENTIDO DE LA REGION DE CONTROL DE LA EXPRESION DEL GEN DE FOSFATASA ACIDA REPRIMIBLE PORTADO SOBRE EL VECTOR, CONTENIENDO DICHO VECTOR REVERSIBLE UN GEN DE LEVADURA Y UN GEN DE E. COLI Y LLEVA LA REGION DE CONTROL DE LA EXPRESION DEL GEN DE FOSFATASA ACIDA REPRIMIBLE DE LEVADURA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP57142460A JPS5931799A (ja) | 1982-08-16 | 1982-08-16 | B型肝炎ウイルス遺伝子を組込んだ組換えプラスミド |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8600059A1 true ES8600059A1 (es) | 1985-10-01 |
ES524942A0 ES524942A0 (es) | 1985-10-01 |
Family
ID=15315829
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES524942A Granted ES524942A0 (es) | 1982-08-16 | 1983-08-12 | Procedimiento para la preparacion de un plasmido recombinante insertado con el gen del virus de la hepatitis b. |
ES540895A Expired ES8802162A1 (es) | 1982-08-16 | 1985-03-01 | Un procedimiento para la preparacion de una levadura trans- formada con un plasmido recombinante insertado con el gen del virus de la hepatitis b. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES540895A Expired ES8802162A1 (es) | 1982-08-16 | 1985-03-01 | Un procedimiento para la preparacion de una levadura trans- formada con un plasmido recombinante insertado con el gen del virus de la hepatitis b. |
Country Status (8)
Country | Link |
---|---|
US (1) | US4778761A (es) |
JP (1) | JPS5931799A (es) |
KR (1) | KR890000096B1 (es) |
CA (1) | CA1270217A (es) |
DK (1) | DK169953B1 (es) |
ES (2) | ES524942A0 (es) |
IL (1) | IL69423A0 (es) |
SU (1) | SU1387878A3 (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
JPS5936699A (ja) * | 1982-08-20 | 1984-02-28 | Chemo Sero Therapeut Res Inst | シヤトルベクタ− |
JPS5974985A (ja) * | 1982-10-19 | 1984-04-27 | Takeda Chem Ind Ltd | 新規dna |
JPS61139391A (ja) * | 1984-12-11 | 1986-06-26 | Chemo Sero Therapeut Res Inst | 単純ヘルペスウィルス遺伝子を組込んだ組換えプラスミドおよび形質転換酵母 |
US4816564A (en) * | 1986-01-31 | 1989-03-28 | Merck & Co., Inc. | Method for producing hepatitis B virus proteins in yeast |
US5190877A (en) * | 1987-09-03 | 1993-03-02 | Gist-Brocades N.V. | Saccharomyces strains for maltose fermentation |
US4883865A (en) * | 1987-09-30 | 1989-11-28 | Merck & Co. Inc. | Recovery of pres2+s antigen |
AP129A (en) * | 1988-06-03 | 1991-04-17 | Smithkline Biologicals S A | Expression of retrovirus gag protein eukaryotic cells |
US6319501B1 (en) * | 1990-07-19 | 2001-11-20 | Health Research, Inc. | Method of immunizing against hepatitis B virus |
LT3988B (en) | 1992-02-17 | 1996-06-25 | Fermentas Biotech Inst | Recombinant plasmides pfs19, pfps2-48 and pjlfds1 codingsynthesis of human hepatite b of surfice virus antigenes, methods fof producing thereof |
US5531990A (en) * | 1993-12-15 | 1996-07-02 | Health Research, Inc. | Method of raising an immune response with an anti-idiotypic antibody having correspondence with human hepatitis B surface antigen |
IL118626A0 (en) * | 1996-06-11 | 1996-10-16 | Xtl Biopharmaceuticals Limited | Anti HBV antibody |
US6451572B1 (en) * | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
DK1165806T3 (da) * | 1999-03-31 | 2005-12-05 | Cornell Res Foundation Inc | Phosphataser med forbedret phytaseaktivitet |
US6841370B1 (en) * | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
BRPI0213813B1 (pt) | 2001-10-31 | 2018-11-21 | Cornell Res Foundation Inc | método para aprimorar o valor nutricional de uma ração consumida por um animal monogástrico e ração |
WO2004024885A2 (en) * | 2002-09-13 | 2004-03-25 | Cornell Research Foundation, Inc. | Using mutations to improve aspergillus phytases |
US7919297B2 (en) | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
US8540984B2 (en) * | 2006-08-03 | 2013-09-24 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
KR101445903B1 (ko) | 2007-05-02 | 2014-09-29 | 메리얼 리미티드 | 향상된 발현 및 안정성을 갖는 dna 플라스미드 |
US8192734B2 (en) | 2007-07-09 | 2012-06-05 | Cornell University | Compositions and methods for bone strengthening |
CN112680464B (zh) * | 2020-12-10 | 2022-04-26 | 南京农业大学 | 一种单甲基砷和三价锑氧化酶基因arsV及其编码的蛋白和应用 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2444713A1 (fr) * | 1978-12-18 | 1980-07-18 | Pasteur Institut | Procede de production d'un adn comprenant le genome du virus de l'hepatite b et vecteur le comportant |
FR2480779B2 (fr) * | 1979-08-30 | 1986-07-18 | Anvar | Vecteur contenant une sequence nucleotidique de l'antigene de surface du virus de l'hepatite b et procede de fabrication d'une molecule immunogene mettant en oeuvre ce vecteur |
US4615974A (en) * | 1981-08-25 | 1986-10-07 | Celltech Limited | Yeast expression vectors |
US4446235A (en) * | 1982-03-22 | 1984-05-01 | Genentech, Inc. | Method for cloning human growth hormone varient genes |
US4546082A (en) * | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
AU584580B2 (en) * | 1982-09-08 | 1989-06-01 | Smith Kline - Rit | Hepatitis B virus vaccine |
CA1341302C (en) * | 1983-02-22 | 2001-10-09 | Rae Lyn Burke | Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein |
-
1982
- 1982-08-16 JP JP57142460A patent/JPS5931799A/ja active Granted
-
1983
- 1983-08-03 IL IL69423A patent/IL69423A0/xx not_active IP Right Cessation
- 1983-08-12 ES ES524942A patent/ES524942A0/es active Granted
- 1983-08-13 KR KR1019830003794A patent/KR890000096B1/ko not_active IP Right Cessation
- 1983-08-15 DK DK372183A patent/DK169953B1/da not_active IP Right Cessation
- 1983-08-15 SU SU833635804A patent/SU1387878A3/ru active
- 1983-08-16 CA CA000434726A patent/CA1270217A/en not_active Expired - Lifetime
-
1985
- 1985-03-01 ES ES540895A patent/ES8802162A1/es not_active Expired
-
1987
- 1987-01-20 US US07/004,424 patent/US4778761A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA1270217A (en) | 1990-06-12 |
KR840005745A (ko) | 1984-11-15 |
DK372183D0 (da) | 1983-08-15 |
DK372183A (da) | 1984-02-17 |
JPS6155950B2 (es) | 1986-11-29 |
US4778761A (en) | 1988-10-18 |
DK169953B1 (da) | 1995-04-10 |
IL69423A0 (en) | 1983-11-30 |
KR890000096B1 (ko) | 1989-03-07 |
JPS5931799A (ja) | 1984-02-20 |
ES524942A0 (es) | 1985-10-01 |
SU1387878A3 (ru) | 1988-04-07 |
ES8802162A1 (es) | 1988-04-01 |
ES540895A0 (es) | 1988-04-01 |
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