ES8602935A1 - Un vector reversible - Google Patents
Un vector reversibleInfo
- Publication number
- ES8602935A1 ES8602935A1 ES525065A ES525065A ES8602935A1 ES 8602935 A1 ES8602935 A1 ES 8602935A1 ES 525065 A ES525065 A ES 525065A ES 525065 A ES525065 A ES 525065A ES 8602935 A1 ES8602935 A1 ES 8602935A1
- Authority
- ES
- Spain
- Prior art keywords
- yeast
- gene
- hbsag
- shuttle vector
- prepn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Saccharide Compounds (AREA)
Abstract
UN PROCEDIMIENTO PARA LA PREPARACION DE UN VECTOR REVERSIBLE LEVADURA E. COLI CON UN PROMOTOR DE FOSFATASA ACIDA REPRIMIBLE. CONSISTE EN COMBINAR 1 UN GEN DE LEVADURA QUE CONTIENE A) UN GEN, CON PROMOTOR INCLUIDO, DE FOSFATASA ACIDA, B) UNA SECUENCIA DE DNA NECESARIA PARA LA REPLICACION AUTONOMA DE LA LEVADURA C) UN GEN UTIL COMO MARCADOR SELECTIVO CON 2 EL PLASMIDO PBR322 DE E. COLI QUE CONTIENE A) UNA SECUENCIA DE DNA NECESARIA PARA LA REPLICACION DEL PLASMIDO DENTRO DE E. COLI B) UN GEN UTIL COMO MARCADOR SELECTIVO, PARA DAR LUGAR A UN PLASMIDO RECOMBINADO, EL CUAL, POR TRATAMIENTO CON UNA ENZIMA DE RESTRICCION, UNA EXONUCLEASA Y POSTERIORMENTE UNA LIGASA, CON ELIMINACION DE 1 A 50BP DEL GEN FOSFATASA ACIDA PERO NO SU PROMOTOR, SE OBTIENE UN VECTOR REVERSIBLE QUE PUEDE EXPRESAR UN GEN EXTRAÑO BAJO EL CONTROL DEL PROMOTOR DE FOSFATASA ACIDA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57145093A JPS5936699A (ja) | 1982-08-20 | 1982-08-20 | シヤトルベクタ− |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES525065A0 ES525065A0 (es) | 1985-12-01 |
| ES8602935A1 true ES8602935A1 (es) | 1985-12-01 |
Family
ID=15377210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES525065A Expired ES8602935A1 (es) | 1982-08-20 | 1983-08-19 | Un vector reversible |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5707862A (es) |
| JP (2) | JPS5936699A (es) |
| KR (1) | KR840005740A (es) |
| AU (1) | AU562633B2 (es) |
| CA (1) | CA1270453A (es) |
| DK (1) | DK381483A (es) |
| ES (1) | ES8602935A1 (es) |
| SU (1) | SU1393317A3 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0811074B2 (ja) * | 1987-10-30 | 1996-02-07 | 財団法人化学及血清療法研究所 | プレアルブミンをコードする遺伝子を組込んだ組換えプラスミドおよびこれを用いたプレアルブミンの製法 |
| JPH0291660U (es) * | 1988-12-28 | 1990-07-20 | ||
| EP1560919A2 (en) * | 2001-03-29 | 2005-08-10 | Genetic Technologies, L.P. | Improved hybrid gene libraries and uses thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2441659A1 (fr) * | 1978-11-14 | 1980-06-13 | Anvar | Nouveaux plasmides hybrides et microorganismes les contenant |
| GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
| JPS5931799A (ja) * | 1982-08-16 | 1984-02-20 | Science & Tech Agency | Ｂ型肝炎ウイルス遺伝子を組込んだ組換えプラスミド |
| IL69202A (en) * | 1982-09-08 | 1991-08-16 | Smith Kline Rit | Surface antigen polypeptides of hbv virus produced in yeast and the preparation thereof |
| CA1341302C (en) * | 1983-02-22 | 2001-10-09 | Rae Lyn Burke | Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein |
| JPH0655146B2 (ja) * | 1985-12-27 | 1994-07-27 | 財団法人化学及血清療法研究所 | シヤトルベクタ− |
-
1982
- 1982-08-20 JP JP57145093A patent/JPS5936699A/ja active Granted
-
1983
- 1983-08-15 AU AU18003/83A patent/AU562633B2/en not_active Expired
- 1983-08-18 KR KR1019830003854A patent/KR840005740A/ko not_active Application Discontinuation
- 1983-08-19 SU SU833635251A patent/SU1393317A3/ru active
- 1983-08-19 ES ES525065A patent/ES8602935A1/es not_active Expired
- 1983-08-19 CA CA000435007A patent/CA1270453A/en not_active Expired - Lifetime
- 1983-08-19 DK DK381483A patent/DK381483A/da not_active Application Discontinuation
-
1986
- 1986-05-10 JP JP61107431A patent/JPH0665308B2/ja not_active Expired - Lifetime
-
1990
- 1990-11-29 US US07/622,344 patent/US5707862A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6155951B2 (es) | 1986-11-29 |
| DK381483A (da) | 1984-02-21 |
| CA1270453A (en) | 1990-06-19 |
| US5707862A (en) | 1998-01-13 |
| AU1800383A (en) | 1984-02-23 |
| ES525065A0 (es) | 1985-12-01 |
| JPH0665308B2 (ja) | 1994-08-24 |
| JPS5936699A (ja) | 1984-02-28 |
| SU1393317A3 (ru) | 1988-04-30 |
| JPS62115289A (ja) | 1987-05-26 |
| KR840005740A (ko) | 1984-11-16 |
| DK381483D0 (da) | 1983-08-19 |
| AU562633B2 (en) | 1987-06-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD1A | Patent lapsed |
Effective date: 20050919 |