ES8506168A1 - Un metodo de detectar una secuencia de polinucleotido. - Google Patents
Un metodo de detectar una secuencia de polinucleotido.Info
- Publication number
- ES8506168A1 ES8506168A1 ES529179A ES529179A ES8506168A1 ES 8506168 A1 ES8506168 A1 ES 8506168A1 ES 529179 A ES529179 A ES 529179A ES 529179 A ES529179 A ES 529179A ES 8506168 A1 ES8506168 A1 ES 8506168A1
- Authority
- ES
- Spain
- Prior art keywords
- radioactive
- chemically
- methods
- polynucleotide probes
- labeled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/552—Glass or silica
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
Abstract
UN METODO DE DETECTAR UNA SECUENCIA DE POLINUCLEOTIDO.COMPRENDE LAS OPERACIONES DE LLEVAR DICHA SECUENCIA DE POLINUCLEOTIDO A UNA FORMA MONOCATENARIA; PONER EN CONTACTO DICHA SECUENCIA DE POLINUCLEOTIDO CON UNA SONDA DE POLINUCLEOTIDO NO RADIACTIVA, QUIMICAMENTE MARCADA, EN FORMA MONOCATENARIA, EN CONDICIONES QUE PERMITAN LA HIBRIDACION, SIENDO DICHA SONDA SUSTANCIALMENTE COMPLEMENTARIA DE DICHA SECUENCIA DE POLINUCLEOTIDO Y SIENDO DICHO MARCADOR QUIMICO NO RADIACTIVO CAPAZ DE FORMAR UNA SEN/AL NO LOCALIZADA; Y DETECTAR DICHA SECUENCIA DE POLINUCLEOTIDO POR MEDIO DE LA FORMACION DE DICHA SEN/AL NO LOCALIZADA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US46146983A | 1983-01-27 | 1983-01-27 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES529179A0 ES529179A0 (es) | 1985-06-16 |
ES8506168A1 true ES8506168A1 (es) | 1985-06-16 |
Family
ID=23832687
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES529179A Expired ES8506168A1 (es) | 1983-01-27 | 1984-01-26 | Un metodo de detectar una secuencia de polinucleotido. |
ES540485A Expired ES8605042A1 (es) | 1983-01-27 | 1985-02-16 | Un metodo de detectar una secuencia de polinucleotido |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES540485A Expired ES8605042A1 (es) | 1983-01-27 | 1985-02-16 | Un metodo de detectar una secuencia de polinucleotido |
Country Status (10)
Country | Link |
---|---|
EP (2) | EP0525821B1 (es) |
JP (1) | JP2825090B2 (es) |
AT (2) | ATE321879T1 (es) |
AU (1) | AU577776B2 (es) |
CA (1) | CA1309672C (es) |
DE (2) | DE3486496D1 (es) |
DK (1) | DK31384A (es) |
ES (2) | ES8506168A1 (es) |
IL (1) | IL70765A (es) |
NO (1) | NO840289L (es) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4994373A (en) | 1983-01-27 | 1991-02-19 | Enzo Biochem, Inc. | Method and structures employing chemically-labelled polynucleotide probes |
CA1254525A (en) * | 1983-04-13 | 1989-05-23 | Christine L. Brakel | Kit for terminally chemically labeling dna |
NO841725L (no) * | 1983-05-02 | 1984-11-05 | Enzo Biochem Inc | Fremgangsmaate og stoffer til bruk ved analyse og paavisning av genetisk materiale |
CA1338856C (en) * | 1983-07-05 | 1997-01-21 | Jannis Stavrianopoulos | In vivo labelling of polynucleotide sequences |
US4943523A (en) * | 1984-01-30 | 1990-07-24 | Enzo Biochem, Inc. | Detectable molecules, method of preparation and use |
US4849505A (en) * | 1984-01-30 | 1989-07-18 | Enzo Biochem, Inc. | Detectable molecules, method of preparation and use |
US4849208A (en) * | 1984-01-30 | 1989-07-18 | Enzo Biochem, Inc. | Detectable molecules, method of preparation and use |
US4707440A (en) * | 1984-01-30 | 1987-11-17 | Enzo Biochem, Inc. | Nucleic acid hybridization assay and detectable molecules useful in such assay |
US4843122A (en) * | 1984-01-30 | 1989-06-27 | Enzo Biochem, Inc. | Detectable molecules, method of preparation and use |
US5002885A (en) * | 1984-01-30 | 1991-03-26 | Enzo Biochem, Inc. | Detectable molecules, method preparation and use |
FR2567523B1 (fr) * | 1984-07-12 | 1987-11-13 | Pasteur Institut | Polynucleotides lies de facon covalente a un support solide, et procede pour leur obtention |
DE3431536A1 (de) * | 1984-08-28 | 1986-03-13 | Boehringer Mannheim Gmbh, 6800 Mannheim | Derivatisierte nucleinsaeure-sequenz, verfahren zu deren herstellung sowie deren verwendung zum nachweis von nucleinsaeuren |
US4828979A (en) * | 1984-11-08 | 1989-05-09 | Life Technologies, Inc. | Nucleotide analogs for nucleic acid labeling and detection |
US4670379A (en) * | 1984-12-19 | 1987-06-02 | E. I. Du Pont De Nemours And Company | Polynucleotide hydridization assays employing catalyzed luminescence |
DE3506703C1 (de) * | 1985-02-26 | 1986-04-30 | Sagax Instrument AB, Sundbyberg | Verfahren zur Sequenzanalyse von Nucleinsaeuren,insbesondere der Desoxyribonucleinsaeure (DNA) und der Ribonucleinsaeure (RNA),sowie Traeger zur Durchfuehrung des Verfahrens und Verfahren zur Herstellung des Traegers |
GB8509880D0 (en) * | 1985-04-17 | 1985-05-22 | Ici Plc | Testing device |
CA1295559C (en) * | 1985-08-13 | 1992-02-11 | Jannis Stavrianopoulos | Method for labeling polynucleotide sequences |
EP0228075B1 (en) * | 1986-01-03 | 1991-04-03 | Molecular Diagnostics, Inc. | Eucaryotic genomic dna dot-blot hybridization method |
EP0245206A1 (en) * | 1986-05-05 | 1987-11-11 | IntraCel Corporation | Analytical method for detecting and measuring specifically sequenced nucleic acid |
JPS638396A (ja) * | 1986-06-30 | 1988-01-14 | Wakunaga Pharmaceut Co Ltd | ポリ標識化オリゴヌクレオチド誘導体 |
BE1000572A4 (fr) * | 1987-05-20 | 1989-02-07 | Block Myron J | Cellule rta, appareil et procede pour titrer un polynucleotide dans un liquide. |
GB2209754A (en) * | 1987-09-17 | 1989-05-24 | Thomas Lee Mattson | Polyaldehydic polynucleotides in use as probes,their preparation and use |
JP2597698B2 (ja) * | 1987-10-28 | 1997-04-09 | ハワード・フローレイ・インスティテュト・オブ・イクスペリメンタル・フィジオロジー・アンド・メディシン | オリゴヌクレオチド‐ポリアミド コンジュゲート |
US5047523A (en) * | 1988-08-02 | 1991-09-10 | Ortho Diagnostic Systems Inc. | Nucleic acid probe for detection of neisseria gonorrhoea |
US5324829A (en) * | 1988-12-16 | 1994-06-28 | Ortho Diagnostic Systems, Inc. | High specific activity nucleic acid probes having target recognition and signal generating moieties |
US5556748A (en) * | 1991-07-30 | 1996-09-17 | Xenopore Corporation | Methods of sandwich hybridization for the quantitative analysis of oligonucleotides |
JPH0560761A (ja) * | 1991-08-30 | 1993-03-12 | Imuno Baion:Kk | 重合体の分析方法 |
US5554501A (en) * | 1992-10-29 | 1996-09-10 | Beckman Instruments, Inc. | Biopolymer synthesis using surface activated biaxially oriented polypropylene |
AU8102694A (en) * | 1993-11-17 | 1995-06-06 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
US5922534A (en) | 1995-03-28 | 1999-07-13 | Hewlett-Packard Company | Dry biochemical assay plate and method for making the same |
US6027879A (en) * | 1995-08-09 | 2000-02-22 | The Regents Of The University Of California | Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe |
US5616465A (en) | 1995-08-09 | 1997-04-01 | The Regents Of The University Of California | Detection and isolation of nucleic acid sequences using competitive hybridization probes |
US6613516B1 (en) | 1999-10-30 | 2003-09-02 | Affymetrix, Inc. | Preparation of nucleic acid samples |
US7166478B2 (en) * | 2002-03-12 | 2007-01-23 | Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. | Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses |
KR101140865B1 (ko) * | 2004-02-17 | 2012-05-03 | 에스케이바이오팜 주식회사 | 1-클로로-6,6-디메틸-2-헵텐-4-인의 연속 제조방법 |
RU2431634C2 (ru) | 2005-03-11 | 2011-10-20 | Говард Флори Инститьют Оф Експериментл Физиолоджи Энд Медсин | Соединения флавоноидов и их применение |
EP3155426B1 (en) | 2014-06-13 | 2023-07-19 | Immudex ApS | General detection and isolation of specific cells by binding of labeled molecules |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS608745B2 (ja) * | 1978-02-14 | 1985-03-05 | 三洋化成工業株式会社 | 免疫活性物質−つや消しガラス複合体,その製造法及び該複合体を含有してなる測定試薬 |
US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
CA1219824A (en) * | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
FR2519005B1 (fr) * | 1981-12-29 | 1985-10-25 | Pasteur Institut | Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn |
CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
-
1984
- 1984-01-23 CA CA000445896A patent/CA1309672C/en not_active Expired - Lifetime
- 1984-01-23 IL IL70765A patent/IL70765A/xx unknown
- 1984-01-24 DK DK31384A patent/DK31384A/da not_active Application Discontinuation
- 1984-01-25 NO NO840289A patent/NO840289L/no unknown
- 1984-01-26 AT AT92114727T patent/ATE321879T1/de not_active IP Right Cessation
- 1984-01-26 EP EP92114727A patent/EP0525821B1/en not_active Revoked
- 1984-01-26 DE DE3486496T patent/DE3486496D1/de not_active Expired - Lifetime
- 1984-01-26 EP EP84100836A patent/EP0117440B1/en not_active Revoked
- 1984-01-26 AU AU23798/84A patent/AU577776B2/en not_active Ceased
- 1984-01-26 ES ES529179A patent/ES8506168A1/es not_active Expired
- 1984-01-26 AT AT84100836T patent/ATE87929T1/de not_active IP Right Cessation
- 1984-01-26 DE DE8484100836T patent/DE3486124T2/de not_active Revoked
- 1984-01-27 JP JP59014165A patent/JP2825090B2/ja not_active Expired - Lifetime
-
1985
- 1985-02-16 ES ES540485A patent/ES8605042A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
DK31384A (da) | 1984-07-28 |
EP0117440B1 (en) | 1993-04-07 |
JP2825090B2 (ja) | 1998-11-18 |
IL70765A (en) | 1988-07-31 |
EP0525821B1 (en) | 2006-03-29 |
DE3486124D1 (de) | 1993-05-13 |
ATE87929T1 (de) | 1993-04-15 |
IL70765A0 (en) | 1984-04-30 |
EP0525821A2 (en) | 1993-02-03 |
AU577776B2 (en) | 1988-10-06 |
ATE321879T1 (de) | 2006-04-15 |
AU2379884A (en) | 1984-08-02 |
JPS59141599A (ja) | 1984-08-14 |
ES540485A0 (es) | 1986-02-16 |
ES529179A0 (es) | 1985-06-16 |
DK31384D0 (da) | 1984-01-24 |
EP0117440A1 (en) | 1984-09-05 |
NO840289L (no) | 1984-07-30 |
DE3486124T2 (de) | 1993-07-15 |
ES8605042A1 (es) | 1986-02-16 |
DE3486496D1 (de) | 2006-05-18 |
CA1309672C (en) | 1992-11-03 |
EP0525821A3 (es) | 1994-03-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM4A | Patent lapsed |
Effective date: 19960506 |