WO2001098533A3 - Artifizielle genetische markierung mit synthetischer dna - Google Patents

Artifizielle genetische markierung mit synthetischer dna Download PDF

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Publication number
WO2001098533A3
WO2001098533A3 PCT/EP2001/006198 EP0106198W WO0198533A3 WO 2001098533 A3 WO2001098533 A3 WO 2001098533A3 EP 0106198 W EP0106198 W EP 0106198W WO 0198533 A3 WO0198533 A3 WO 0198533A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
artificial sequence
relates
synthetic dna
organism
Prior art date
Application number
PCT/EP2001/006198
Other languages
English (en)
French (fr)
Other versions
WO2001098533A9 (de
WO2001098533A2 (de
Inventor
Hubert S Bernauer
Original Assignee
Hubert S Bernauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hubert S Bernauer filed Critical Hubert S Bernauer
Priority to EP01949371A priority Critical patent/EP1315834A2/de
Priority to AU2001270545A priority patent/AU2001270545A1/en
Publication of WO2001098533A2 publication Critical patent/WO2001098533A2/de
Publication of WO2001098533A9 publication Critical patent/WO2001098533A9/de
Publication of WO2001098533A3 publication Critical patent/WO2001098533A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zum (gleichzeitigen) Nachweis einer oder mehrerer in einen oder mehrere Organismen oder in eine oder mehrere Zellen fremd eingeführten Nucleinsäure(n), wobei die eingeführten(n) Nucleinsäure(n) (eine) artifizielle Sequenz(en) umfaßt/umfassen, die den Nachweis der Identität der eingeführten Nucleinsäure(n) und die selektive Vermehrung erlaubt/erlauben, umfassend die Untersuchung des Organismus/der Organismen oder der Zelle(n) auf das Vorhandensein der artifiziellen Sequenz(en), und gegebenenfalls Identifizierung der artifiziellen Sequenz(en) mittels Hybridisierung mit einem Chip und/oder Sequenzierung. Die Erfindung betrifft außerdem ein Verfahren zum Nachweis einer in einen Organismus oder eine Zelle fremd eingeführten Nucleinsäure, wobei die eingeführte Nucleinsäure mindestens eine artifizielle Sequenz umfaßt, die mit mindestens einem universellen Primer unter stringenten Hybridisierungsbedingungen hybridisiert. Schliesslich betrifft die Erfindung einen Chip, der die Durchführung der erfindungsgemässen Verfahren erlaubt.
PCT/EP2001/006198 2000-05-31 2001-05-31 Artifizielle genetische markierung mit synthetischer dna WO2001098533A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01949371A EP1315834A2 (de) 2000-05-31 2001-05-31 Artifizielle genetische markierung mit synthetischer dna
AU2001270545A AU2001270545A1 (en) 2000-05-31 2001-05-31 Artificial marking using synthetic dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10027218A DE10027218A1 (de) 2000-05-31 2000-05-31 Artifizielle genetische Markierung mit synthetischer DNA
DE10027218.5 2000-05-31

Publications (3)

Publication Number Publication Date
WO2001098533A2 WO2001098533A2 (de) 2001-12-27
WO2001098533A9 WO2001098533A9 (de) 2002-09-19
WO2001098533A3 true WO2001098533A3 (de) 2003-04-10

Family

ID=7644364

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/006198 WO2001098533A2 (de) 2000-05-31 2001-05-31 Artifizielle genetische markierung mit synthetischer dna

Country Status (4)

Country Link
EP (1) EP1315834A2 (de)
AU (1) AU2001270545A1 (de)
DE (1) DE10027218A1 (de)
WO (1) WO2001098533A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10321480B4 (de) * 2003-05-13 2005-07-21 Universität Potsdam Verfahren zur Identifizierung von Zellinien
CN100370035C (zh) * 2005-09-08 2008-02-20 中国疾病预防控制中心营养与食品安全所 转基因农产品dna检测芯片及其制备方法和应用
CN106367485B (zh) 2016-08-29 2019-04-26 厦门艾德生物医药科技股份有限公司 一种用于检测基因突变的多定位双标签接头组及其制备方法和应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006249A1 (en) * 1993-08-20 1995-03-02 Biocode, Inc. Marking of products to establish identity and source
EP0761822A2 (de) * 1995-09-12 1997-03-12 The Johns Hopkins University School Of Medicine Reihenanalyse-Verfahren der Genexpression
US5846719A (en) * 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
WO1998055657A1 (en) * 1997-06-05 1998-12-10 Cellstore Methods and reagents for indexing and encoding nucleic acids
WO1999055886A1 (en) * 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006249A1 (en) * 1993-08-20 1995-03-02 Biocode, Inc. Marking of products to establish identity and source
US5846719A (en) * 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
EP0761822A2 (de) * 1995-09-12 1997-03-12 The Johns Hopkins University School Of Medicine Reihenanalyse-Verfahren der Genexpression
WO1998055657A1 (en) * 1997-06-05 1998-12-10 Cellstore Methods and reagents for indexing and encoding nucleic acids
WO1999055886A1 (en) * 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BBC NEWS: "DNA hides spy message", BBC NEWS, 10 June 1999 (1999-06-10), pages 1 - 2, XP002225944, Retrieved from the Internet <URL:http://news.bbc.co.uk/1/hi/sci/tech/365183.stm> [retrieved on 20021220] *

Also Published As

Publication number Publication date
DE10027218A1 (de) 2001-12-06
EP1315834A2 (de) 2003-06-04
AU2001270545A1 (en) 2002-01-02
WO2001098533A9 (de) 2002-09-19
WO2001098533A2 (de) 2001-12-27

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