ES8205063A1 - Procedimiento para la determinacion cuantitativa, inmunologica de enzimas, especialmente de las isoenzinas de la fosfa- tasa acida y alcalina en liquido corporales. - Google Patents

Procedimiento para la determinacion cuantitativa, inmunologica de enzimas, especialmente de las isoenzinas de la fosfa- tasa acida y alcalina en liquido corporales.

Info

Publication number
ES8205063A1
ES8205063A1 ES498063A ES498063A ES8205063A1 ES 8205063 A1 ES8205063 A1 ES 8205063A1 ES 498063 A ES498063 A ES 498063A ES 498063 A ES498063 A ES 498063A ES 8205063 A1 ES8205063 A1 ES 8205063A1
Authority
ES
Spain
Prior art keywords
enzyme
antibodies
enzymes
reagent
immunological determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES498063A
Other languages
English (en)
Other versions
ES498063A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of ES8205063A1 publication Critical patent/ES8205063A1/es
Publication of ES498063A0 publication Critical patent/ES498063A0/es
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA DETERMINACION CUANTITATIVA INMUNOLOGICA DE ENZIMAS, ESPECIALMENTE DE LA FOSFATASA ACIDA Y ALCALINA EN LIQUIDOS CORPORALES. CONSISTE EN LA INCUBACION DE LOS ANTICUERPOS CONTRA LA ENZIMA A DETERMINAR, ABSORBIDOS EN UN SOPORTE INSOLUBLE EN AGUA, CON LA SOLUCION DE MUESTRA QUE CONTIENE ESTA ENZIMA, DESPUES LA DISOLUCION DE MUESTRA, SE LAVA EL SOPORTE DE ANTICUERPOS CARGADO CON ENZIMAS Y SE DETERMINA LA ACTIVIDAD DE LA ENZIMA UNIDA A LOS ANTICUERPOS. ESTA DETERMINACION DE ENZIMAS TIENE APLICACIONES PARA LA DIAGNOSIS DEFERENCIAL DE DISTINTAS ENFERMEDADES INTERNAS DEL SER HUMANO
ES498063A 1979-12-27 1980-12-22 Procedimiento para la determinacion cuantitativa, inmunologica de enzimas, especialmente de las isoenzinas de la fosfa- tasa acida y alcalina en liquido corporales. Granted ES498063A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19792952478 DE2952478A1 (de) 1979-12-27 1979-12-27 Verfahren und mittel zur immunologischen bestimmung von enzymen

Publications (2)

Publication Number Publication Date
ES8205063A1 true ES8205063A1 (es) 1982-05-16
ES498063A0 ES498063A0 (es) 1982-05-16

Family

ID=6089742

Family Applications (1)

Application Number Title Priority Date Filing Date
ES498063A Granted ES498063A0 (es) 1979-12-27 1980-12-22 Procedimiento para la determinacion cuantitativa, inmunologica de enzimas, especialmente de las isoenzinas de la fosfa- tasa acida y alcalina en liquido corporales.

Country Status (9)

Country Link
US (1) US4366242A (es)
EP (1) EP0032204B1 (es)
JP (1) JPS5698656A (es)
AT (1) ATE14631T1 (es)
CS (1) CS237318B2 (es)
DD (1) DD201193A5 (es)
DE (2) DE2952478A1 (es)
ES (1) ES498063A0 (es)
ZA (1) ZA808051B (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4446231A (en) * 1979-10-03 1984-05-01 Self Colin H Immunoassay using an amplified cyclic detection system
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
US4849353A (en) * 1980-09-30 1989-07-18 Cornell Research Foundation, Inc. Immunocapture of enzyme inhibitor, enzyme complexes and uses thereof
US4592995A (en) * 1981-03-30 1986-06-03 Dainippon Pharmaceutical Co., Ltd. Reagent for streptococcal anti-esterase assay
CA1185525A (en) * 1981-06-22 1985-04-16 Hans J. Hager Immunochemical assay for prostatic acid phosphatase
JPS5843798A (ja) * 1981-09-09 1983-03-14 Mamoru Sugiura ヒト尿中カリクレインの活性測定用試薬
EP0131606B1 (de) * 1983-01-12 1986-11-05 Roche Diagnostics GmbH Verfahren und reagenz zur differenzierten bestimmung von isoenzymen der alkalischen phosphatase
US4629694A (en) * 1983-07-12 1986-12-16 Cornell Research Foundation, Inc. Detecting and distinguishing between plasminogen activators
US4778755A (en) * 1983-08-08 1988-10-18 Minnesota Mining And Manufacturing Company Immunoassay method
GB8328918D0 (en) * 1983-10-28 1983-11-30 Unilever Plc Alkaline phosphatase
DE3420926A1 (de) * 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zur differenzierten bestimmung von isoenzymen der alkalischen phosphatase sowie hierzu geeigneter monoklonaler antikoerper
DE3445933A1 (de) * 1984-12-17 1986-06-19 Röhm GmbH, 6100 Darmstadt Arzneimittel mit diuretischer wirksamkeit
FR2582103B1 (fr) * 1985-03-19 1989-05-05 Canavaggio Michel Procede de determination d'une substance biologique dans un echantillon
FI89634C (fi) * 1987-07-10 1993-10-25 Yamanouchi Pharma Co Ltd Foerfarande, anordning och kitt foer maetning av vaevnadsplasminogenaktivator-aktivitet
US5447837A (en) * 1987-08-05 1995-09-05 Calypte, Inc. Multi-immunoassay diagnostic system for antigens or antibodies or both
US5320943A (en) * 1991-03-29 1994-06-14 La Jolla Cancer Research Foundation Methods of detecting T-cell dysfunctions
US7981694B2 (en) * 2003-01-28 2011-07-19 The Regents Of The University Of California Solid phase isolation of proteins, nucleic acids and other macromolecules
EP3431996A1 (en) * 2017-07-17 2019-01-23 PreviPharma Consulting GmbH Quantifying enzymatic entities and their active sites

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2128670B2 (de) * 1971-06-09 1977-06-30 Merck Patent Gmbh, 6100 Darmstadt Immunologische isoenzym-bestimmungsmethode
NL7309558A (es) * 1972-07-10 1974-01-14
DE2258822A1 (de) * 1972-12-01 1974-06-06 Merck Patent Gmbh Verfahren und mittel zur analyse von isoenzymmustern
US3966898A (en) * 1973-06-28 1976-06-29 Pharmacia Diagnostics Ab Method and reagent for determining immunologic materials
FR2253552A1 (es) * 1973-12-07 1975-07-04 Miles Lab
US4016043A (en) * 1975-09-04 1977-04-05 Akzona Incorporated Enzymatic immunological method for the determination of antigens and antibodies
DE2861945D1 (en) * 1978-01-04 1982-08-26 Research Corp Composition and a diagnostic method for the detection of prostatic cancer
US4260678A (en) * 1979-02-23 1981-04-07 Corning Glass Works Determining creatine kinase isoenzmes via immobilized antibody-isoenzyme complexes
US4298592A (en) * 1979-04-05 1981-11-03 Mallinckrodt, Inc. Double antibody separation method
US4267272A (en) * 1979-11-05 1981-05-12 Baxter Travenol Laboratories, Inc. Prostatic acid phosphatase immuno assay

Also Published As

Publication number Publication date
EP0032204A1 (de) 1981-07-22
DD201193A5 (de) 1983-07-06
DE2952478A1 (de) 1981-07-30
CS906380A2 (en) 1984-12-14
CS237318B2 (en) 1985-07-16
DE3070938D1 (en) 1985-09-05
EP0032204B1 (de) 1985-07-31
ATE14631T1 (de) 1985-08-15
JPS5698656A (en) 1981-08-08
ES498063A0 (es) 1982-05-16
US4366242A (en) 1982-12-28
JPH0116388B2 (es) 1989-03-24
ZA808051B (en) 1982-01-27

Similar Documents

Publication Publication Date Title
ES8205063A1 (es) Procedimiento para la determinacion cuantitativa, inmunologica de enzimas, especialmente de las isoenzinas de la fosfa- tasa acida y alcalina en liquido corporales.
AU574281B2 (en) Process for the quantification of cell populations or subpopulations and a reagent suitable therefor
ES483313A1 (es) Procedimiento de determinacion inmunologico para un complejoinmunologico
DE3769664D1 (de) Heterogener spezifischer bindungstest unter verwendung eines anhaeufbaren bindungsreagens.
ES8900067A1 (es) Un metodo y su correspondiente dispositivo para la determinacion de anticuerpos en liquidos biologicos.
ES467831A1 (es) Procedimiento para la preparacion de un reactivo para deter-minar un material inmunologicamente activo
EP0113075A3 (en) Field immunoassay reaction system, method of immunoassay diagnosis and probe or carrier for use therewith
DE3888779D1 (de) Immuntestsatz und -verfahren für ganze Zellen.
JPS57179750A (en) Single culture immunity chemical examining method
US4188371A (en) Immunological detection of Neisseria bacteria via labelled antibodies
GB1527397A (en) Serum hepatitis test
EP0238631A4 (en) IMMUNOLOGICAL ANALYSIS KIT AND METHOD.
CA1122119A (en) Process for the determination of the thyroxine- binding index in serum
IT1212845B (it) Saggio immunologico utilizzante un supporto cromogeno.
KR940000870A (ko) β-N- 아세틸글루코사미니다제의 샌드위치 면역 측정법 및 그 측정법에 사용된 모노클론 항체
GB1508133A (en) Biological analysis
EP0315447A3 (en) Method of immunological measurement of human protein s and reagent and kit therefor
JPS6463864A (en) Immunoassay
JPS57131062A (en) Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier
DE3477610D1 (en) Method for the immunochemical determination of hepatitis b core antigens
JPS6450963A (en) Uniform immunoassay using riposome
ES8205316A1 (es) Procedimiento para la determinacion inmunologica de anticuerpos, antigenos y haptenos por efecto comunicador de una ac- tividad enzimatica.
JPS5858467A (ja) 免疫学的測定法
JPS6463863A (en) Immunoassay
JPS6474452A (en) Reagent sealing body