JPS6463863A - Immunoassay - Google Patents

Immunoassay

Info

Publication number
JPS6463863A
JPS6463863A JP22079687A JP22079687A JPS6463863A JP S6463863 A JPS6463863 A JP S6463863A JP 22079687 A JP22079687 A JP 22079687A JP 22079687 A JP22079687 A JP 22079687A JP S6463863 A JPS6463863 A JP S6463863A
Authority
JP
Japan
Prior art keywords
antibody
antigen
porous membrane
specimen
labelled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP22079687A
Other languages
Japanese (ja)
Inventor
Masao Kitajima
Shigeki Kageyama
Toshihiro Hiraoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Holdings Corp
Original Assignee
Fuji Photo Film Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Photo Film Co Ltd filed Critical Fuji Photo Film Co Ltd
Priority to JP22079687A priority Critical patent/JPS6463863A/en
Priority to EP88114356A priority patent/EP0308704A1/en
Publication of JPS6463863A publication Critical patent/JPS6463863A/en
Pending legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To simplify measuring operation, by competitively reacting an antigen or antibody labelled with enzyme and a specimen on a porous membrane having an antibody or antigen insolubilized thereon and measuring the enzymatic activity of a liquid phase using a dry chemical analysis film. CONSTITUTION:At first, a definite amount of an antibody or antigen is physically or chemically bonded to a porous membrane. The non-specific bonding region of the surface of the porous membrane is blocked by gelatin. A specimen and a definite amount of an enzyme labelled antigen (or antibody) is reacted with the porous membrane. Hereupon, the antigen (or antibody) in the specimen and the labelled antigen (or antibody) are competitively reacted with the insolubilized antibody (or antigen). Then, the amount of the non-bonding type enzyme labelled antigen (or antibody) present in the liquid phase after reaction is measured by a reaction speed method using a dry chemical analysis film containing all of reagents necessary for the measurement of enzymatic activity.
JP22079687A 1987-09-03 1987-09-03 Immunoassay Pending JPS6463863A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP22079687A JPS6463863A (en) 1987-09-03 1987-09-03 Immunoassay
EP88114356A EP0308704A1 (en) 1987-09-03 1988-09-02 Heterogeneous enzymimmunoassay

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP22079687A JPS6463863A (en) 1987-09-03 1987-09-03 Immunoassay

Publications (1)

Publication Number Publication Date
JPS6463863A true JPS6463863A (en) 1989-03-09

Family

ID=16756701

Family Applications (1)

Application Number Title Priority Date Filing Date
JP22079687A Pending JPS6463863A (en) 1987-09-03 1987-09-03 Immunoassay

Country Status (1)

Country Link
JP (1) JPS6463863A (en)

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