JPS6463863A - Immunoassay - Google Patents
ImmunoassayInfo
- Publication number
- JPS6463863A JPS6463863A JP22079687A JP22079687A JPS6463863A JP S6463863 A JPS6463863 A JP S6463863A JP 22079687 A JP22079687 A JP 22079687A JP 22079687 A JP22079687 A JP 22079687A JP S6463863 A JPS6463863 A JP S6463863A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- antigen
- porous membrane
- specimen
- labelled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To simplify measuring operation, by competitively reacting an antigen or antibody labelled with enzyme and a specimen on a porous membrane having an antibody or antigen insolubilized thereon and measuring the enzymatic activity of a liquid phase using a dry chemical analysis film. CONSTITUTION:At first, a definite amount of an antibody or antigen is physically or chemically bonded to a porous membrane. The non-specific bonding region of the surface of the porous membrane is blocked by gelatin. A specimen and a definite amount of an enzyme labelled antigen (or antibody) is reacted with the porous membrane. Hereupon, the antigen (or antibody) in the specimen and the labelled antigen (or antibody) are competitively reacted with the insolubilized antibody (or antigen). Then, the amount of the non-bonding type enzyme labelled antigen (or antibody) present in the liquid phase after reaction is measured by a reaction speed method using a dry chemical analysis film containing all of reagents necessary for the measurement of enzymatic activity.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP22079687A JPS6463863A (en) | 1987-09-03 | 1987-09-03 | Immunoassay |
EP88114356A EP0308704A1 (en) | 1987-09-03 | 1988-09-02 | Heterogeneous enzymimmunoassay |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP22079687A JPS6463863A (en) | 1987-09-03 | 1987-09-03 | Immunoassay |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6463863A true JPS6463863A (en) | 1989-03-09 |
Family
ID=16756701
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP22079687A Pending JPS6463863A (en) | 1987-09-03 | 1987-09-03 | Immunoassay |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6463863A (en) |
-
1987
- 1987-09-03 JP JP22079687A patent/JPS6463863A/en active Pending
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