ES8105777A1 - Un procedimiento para la produccion de un plasmido. - Google Patents

Un procedimiento para la produccion de un plasmido.

Info

Publication number
ES8105777A1
ES8105777A1 ES492053A ES492053A ES8105777A1 ES 8105777 A1 ES8105777 A1 ES 8105777A1 ES 492053 A ES492053 A ES 492053A ES 492053 A ES492053 A ES 492053A ES 8105777 A1 ES8105777 A1 ES 8105777A1
Authority
ES
Spain
Prior art keywords
plasmid vectors
dna fragment
site
translation
disclosed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES492053A
Other languages
English (en)
Other versions
ES492053A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GD Searle LLC
Original Assignee
GD Searle LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GD Searle LLC filed Critical GD Searle LLC
Publication of ES8105777A1 publication Critical patent/ES8105777A1/es
Publication of ES492053A0 publication Critical patent/ES492053A0/es
Granted legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA PRODUCCION DE UN PLASMIDO CON UN CENTRO DE INSERCION PARA UN FRAGMENTO DE ADN EUCARIOTICO O CON UN FRAGMENTO DE ADN EUCARIOTICO INSERTADO EN DICHO CENTRO, ADYACENTE A UN PROMOTOR BACTERIANO Y MAS ABAJO DE UN CENTRO COMBINANTE DE RIBOSOMA PROCARIOTICO Y DE UN CODON INICIADOR, DE MANERA QUE EL PROMOTOR BACTERIANO CONTROLE LA TRANSCRIPCION Y LA TRADUCCION DEL FRAGMENTO DE ADN INSERTADO. EL PROCESO CONSTA DE LAS SIGUIENTES ETAPAS: PRIMERA, CLONAR UN PROMOTOR BACTERIANO EN UN PLASMIDO AISLADO DETERMINANDO LA POSICION DEL CENTRO DE INSERCION PRETENDIDO Y PROPORCIONANDO EL CENTRO DE INSERCION SI AUN NO ESTA PRESENTE; SEGUNDA, OPCIONALMENTE, SEPARAR EL CENTRO NIC DEL PLASMIDO OBTENIDO EN LA ETAPA PRIMERA O EFECTUAR DICHA ETAPA DE TAL MODO QUE EL CENTRO NIC NO ESTE PRESENTE, Y POR ULTIMO, OPCIONALMENTE, INSERTAR UN FRAGMENTO DE ADN EUCARIOTICO EN EL CENTRO DE INSERCION DEL PLASMIDO RESULTANTE DE LA ETAPA PRIMERA U, OPCIONALMENTE, DE LA ETAPA SEGUNDA.
ES492053A 1979-06-01 1980-05-30 Un procedimiento para la produccion de un plasmido. Granted ES492053A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB7919245 1979-06-01

Publications (2)

Publication Number Publication Date
ES8105777A1 true ES8105777A1 (es) 1981-06-16
ES492053A0 ES492053A0 (es) 1981-06-16

Family

ID=10505591

Family Applications (1)

Application Number Title Priority Date Filing Date
ES492053A Granted ES492053A0 (es) 1979-06-01 1980-05-30 Un procedimiento para la produccion de un plasmido.

Country Status (15)

Country Link
US (1) US4349629A (es)
JP (1) JPS5636500A (es)
AU (1) AU542264B2 (es)
BE (1) BE883564A (es)
CA (1) CA1168602A (es)
CH (1) CH653705A5 (es)
DE (1) DE3020528A1 (es)
DK (1) DK159976C (es)
ES (1) ES492053A0 (es)
FR (1) FR2457894B1 (es)
GB (1) GB2052516B (es)
IE (1) IE49989B1 (es)
IT (1) IT1147085B (es)
NL (1) NL8003171A (es)
SE (1) SE454596B (es)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4357421A (en) * 1979-04-02 1982-11-02 G. D. Searle & Co. Synthetic gene coding for influenza hemagglutinin
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
YU45104B (en) 1980-04-03 1992-03-10 Biogen Nv Process for producing recombinant dnk molecule, capable of inducting polypeptide expression in an one-cell host
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
US5525484A (en) * 1981-01-16 1996-06-11 Genome Therapeutics Corp. Recombinant DNA means and method for producing rennin, prorenin and pre-prorennin
JPS57134500A (en) * 1981-02-12 1982-08-19 Kyowa Hakko Kogyo Co Ltd Plasmid pcg1
NZ199722A (en) * 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
JPS57181098A (en) * 1981-04-30 1982-11-08 Japan Found Cancer Novel recombinant dna
JPS58110600A (ja) * 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
US5236831A (en) * 1981-12-29 1993-08-17 Kiowa Hakko Kogyo Co., Ltd. Amino acid synthesis in corynebacteria using E. coli genes
US6936694B1 (en) 1982-05-06 2005-08-30 Intermune, Inc. Manufacture and expression of large structural genes
JPS60145685A (ja) 1984-01-09 1985-08-01 Nec Corp 分布帰還型半導体レ−ザ
JPS592689A (ja) * 1982-06-04 1984-01-09 Handai Biseibutsubiyou Kenkyukai 強力な遺伝子発現能を有する新規レプリコンの作成法
US4891315A (en) * 1982-10-25 1990-01-02 American Cyanamid Company Production of herpes simplex viral porteins
US4559302A (en) * 1982-11-01 1985-12-17 Eli Lilly And Company DNA for directing transcription and expression of structural genes
US4634678A (en) * 1982-12-13 1987-01-06 Molecular Genetics Research And Development Limited Partnership Plasmid cloning and expression vectors for use in microorganisms
US4935370A (en) * 1983-12-23 1990-06-19 Pfizer Inc. Expression plasmids for improved production of heterologous protein in bacteria
US4663281A (en) * 1984-03-22 1987-05-05 Mass Institute Of Technology Enhanced production of proteinaceous materials in eucaryotic cells
IT1198443B (it) * 1984-03-30 1988-12-21 Serono Ist Farm Espressione genetica di polipeptidi ibridi contenenti la sequenza della somatostatina
US5268278A (en) * 1984-03-30 1993-12-07 Istituto Farmacologico Serono S.P.A. Genetic expression of somatostatin as hybrid polypeptide
US4722840A (en) * 1984-09-12 1988-02-02 Chiron Corporation Hybrid particle immunogens
JP2624470B2 (ja) * 1984-10-02 1997-06-25 バイオジェン インコーポレイテッド ストレプトアビジン様ポリペプチドの製造
US4766072A (en) * 1985-07-17 1988-08-23 Promega Corporation Vectors for in vitro production of RNA copies of either strand of a cloned DNA sequence
JP2564268B2 (ja) * 1985-08-28 1996-12-18 協和醗酵工業株式会社 融合抗原ポリペプチド
US4943527A (en) * 1985-10-04 1990-07-24 California Biotechnology Inc. Mature apoai protein production under serum free culturing conditions
EP0239631A4 (en) * 1985-10-04 1989-01-12 Biotech Res Partners Ltd RECOMBINANT APOLIPOPROTEINS AND METHODS.
AU582288B2 (en) * 1986-03-07 1989-03-16 Damon Biotech Inc. Vector and method for achieving high level expression in eukaryotic cells
EP0254249B1 (en) * 1986-07-22 1992-08-19 Ciba-Geigy Ag Preparation of binding factor related polypeptides
DE3629890A1 (de) * 1986-08-29 1988-03-10 Schering Ag Mikroorganismen und plasmide fuer die 2,4-dichlorphenoxyessigsaeure (2,4-d)-monooxigenase - bildung und verfahren zur herstellung dieser plasmide und staemme
US6018030A (en) 1986-11-04 2000-01-25 Protein Polymer Technologies, Inc. Peptides comprising repetitive units of amino acids and DNA sequences encoding the same
GB2204042B (en) * 1987-03-13 1990-10-10 Green Cross Corp A csf-1 human nucleotide sequence used as a gene promoter
JPH0227993A (ja) * 1988-07-15 1990-01-30 Nippon Shinyaku Co Ltd hEGFの製法
US5082767A (en) * 1989-02-27 1992-01-21 Hatfield G Wesley Codon pair utilization
GB2253852B (en) * 1991-02-26 1995-03-22 Ici Plc Vector for expression of heterologous polypeptide comprising inducible selection system
US20040038303A1 (en) * 2002-04-08 2004-02-26 Unger Gretchen M. Biologic modulations with nanoparticles
AU2007352618B2 (en) 2007-05-02 2013-07-18 Boehringer Ingelheim Animal Health USA Inc. DNA plasmids having improved expression and stability
JP6679593B2 (ja) 2014-09-03 2020-04-15 ジーンセグエス,インコーポレイテッド 療法用ナノ粒子および関連する組成物、方法、およびシステム

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
BR7807290A (pt) * 1977-11-08 1979-06-12 Genentech Inc Plasmideo,processo para producao de um polipeptido especifico veiculo clonal,cultura bacteriana transformante,processo para produzir uma substancia imunogenica processo para preparar um gene estrutural,e polidesoxirribonucleotido de faixa dupla
FR2428075A1 (fr) * 1978-06-08 1980-01-04 Pasteur Institut Procede de production de proteines par expression des genes correspondants dans des bacteries et vecteurs susceptibles d'etre mis en oeuvre dans de tels procedes
GB2033905B (en) * 1978-08-21 1982-10-13 Upjohn Co Bacterial preparation of proteins
US4357421A (en) * 1979-04-02 1982-11-02 G. D. Searle & Co. Synthetic gene coding for influenza hemagglutinin
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
IE50833B1 (en) * 1980-01-30 1986-07-23 Searle & Co Recombinant dna techniques
DK33181A (da) * 1980-02-06 1981-08-07 Searle & Co Fremgangsmaade til fremstilling af et interferonlignende protein

Also Published As

Publication number Publication date
IE801109L (en) 1980-12-01
NL8003171A (nl) 1980-12-03
US4349629A (en) 1982-09-14
SE454596B (sv) 1988-05-16
DK159976B (da) 1991-01-07
GB2052516B (en) 1983-06-29
CA1168602A (en) 1984-06-05
IT1147085B (it) 1986-11-19
DK228780A (da) 1981-01-16
DK159976C (da) 1991-05-27
BE883564A (fr) 1980-12-01
GB2052516A (en) 1981-01-28
CH653705A5 (fr) 1986-01-15
JPS5636500A (en) 1981-04-09
SE8004066L (sv) 1980-12-02
DE3020528A1 (de) 1980-12-18
FR2457894B1 (fr) 1985-11-15
IT8048841A0 (it) 1980-05-30
IE49989B1 (en) 1986-01-22
ES492053A0 (es) 1981-06-16
DE3020528C2 (es) 1990-05-03
FR2457894A1 (fr) 1980-12-26
AU5878380A (en) 1980-12-04
AU542264B2 (en) 1985-02-14

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Legal Events

Date Code Title Description
MM4A Patent lapsed

Effective date: 19960401