ES8105151A1 - Un metodo para la preparacion de un anticuerpo monoclonico. - Google Patents
Un metodo para la preparacion de un anticuerpo monoclonico.Info
- Publication number
- ES8105151A1 ES8105151A1 ES490943A ES490943A ES8105151A1 ES 8105151 A1 ES8105151 A1 ES 8105151A1 ES 490943 A ES490943 A ES 490943A ES 490943 A ES490943 A ES 490943A ES 8105151 A1 ES8105151 A1 ES 8105151A1
- Authority
- ES
- Spain
- Prior art keywords
- diagnostic
- monoclonal antibody
- cells
- cell line
- preparing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/3061—Blood cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
- C07K16/2812—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD4
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Hybrid Cells (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PROCEDIMIENTO DE OBTENCION DE UN ANTICUERPO MONOCLONICO. CONSTA DE LAS SIGUIENTES ETAPAS: 1. INMUNIZAR RATONES CON CELULAS T HUMANAS PURIFICADAS POSITIVAS A ROSETRE E. 2. SEPARAR LOS BAZOS DE DICHOS RATONES Y CONFECCIONAR UNA SUSPENSION DE CELULAS DE BAZO. 3. FUSIONAR DICHAS CELULAS DE BAZO CON CELULAS DE MIELOMA DE RATON EN PRESENCIA DE UN PROMOTOR DE LA FUSION. 4. DILUIR Y CULTIVAR LAS CELULAS FUSIONADAS EN POCILLOS SEPARADOS EN UN MEDIO QUE NO SOPORTARA LAS CELULAS DE MIELOMA NO FUSIONADAS. 5. EVALUAR EL SOBRENADANTE EN CADA POCILLO QUE CONTIENE UN HIBRIDOMA EN RELACION CON LA PRESENCIA DE UN ANTICUERPO PARA CELULAS T PURIFICADAS. 6. SELECCIONAR Y CLONAR UN HIBRIDOMA PRODUCTOR DE UN ANTICUERPO QUE REACCIONA CON TODAS LAS CELULAS T AUXILIARES PERIFERICAS HUMANAS NORMALES, PERO NO CON LAS CELULAS B PERIFERICAS HUMANAS NORMALES, LAS CELULAS NULAS O LOS MACROFAGOS. C
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/033,639 US4381295A (en) | 1979-04-26 | 1979-04-26 | Monoclonal antibody to human helper T cells and methods of preparing same |
Publications (2)
Publication Number | Publication Date |
---|---|
ES490943A0 ES490943A0 (es) | 1981-05-16 |
ES8105151A1 true ES8105151A1 (es) | 1981-05-16 |
Family
ID=21871570
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES490943A Expired ES8105151A1 (es) | 1979-04-26 | 1980-04-25 | Un metodo para la preparacion de un anticuerpo monoclonico. |
Country Status (22)
Country | Link |
---|---|
US (1) | US4381295A (es) |
EP (1) | EP0018794B1 (es) |
JP (2) | JPS55145616A (es) |
AT (1) | ATE14454T1 (es) |
AU (1) | AU544792B2 (es) |
CA (1) | CA1170592A (es) |
DE (2) | DE18794T1 (es) |
DK (1) | DK154087C (es) |
ES (1) | ES8105151A1 (es) |
FI (1) | FI75363C (es) |
GR (1) | GR67298B (es) |
HK (1) | HK23786A (es) |
IE (1) | IE50459B1 (es) |
IL (1) | IL59919A (es) |
MY (1) | MY8600513A (es) |
NO (1) | NO159883C (es) |
NZ (1) | NZ193470A (es) |
PH (1) | PH16656A (es) |
PT (1) | PT71148B (es) |
SG (1) | SG6586G (es) |
YU (1) | YU44957B (es) |
ZA (1) | ZA802524B (es) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4381292A (en) * | 1980-11-14 | 1983-04-26 | The Board Of Trustees Of The Leland Stanford Jr. University | Anti-human T-lymphocyte monoclonal antibody |
US4824432A (en) * | 1981-03-24 | 1989-04-25 | S.V.S. Laboratories, Inc. | Method for treating AIDS and other immune deficiencies and immune disorders |
US4474893A (en) * | 1981-07-01 | 1984-10-02 | The University of Texas System Cancer Center | Recombinant monoclonal antibodies |
USRE32011E (en) * | 1981-12-14 | 1985-10-22 | Scripps Clinic And Research Foundation | Ultrapurification of factor VIII using monoclonal antibodies |
US4511662A (en) * | 1982-06-18 | 1985-04-16 | Bio-Rad Laboratories, Inc. | Simultaneous assay for T and B lymphocyte populations and subpopulations |
US4443427A (en) * | 1982-06-21 | 1984-04-17 | Sidney Farber Cancer Institute, Inc. | Monoclonal antibody |
US4582790A (en) * | 1983-02-15 | 1986-04-15 | Wisconsin Alumni Research Foundation | Monoclonal antibody to angiotension-converting enzyme and methods of preparing and using same |
US4550086A (en) * | 1983-02-16 | 1985-10-29 | Dana-Farber Cancer Institute, Inc. | Monoclonal antibodies that recognize human T cells |
US4677056A (en) * | 1983-08-18 | 1987-06-30 | Sloan-Kettering Institute For Cancer Research | Monoclonal antibody subsetting human helper and killer T-cells and method |
USRE35277E (en) * | 1983-09-11 | 1996-06-18 | Yeda Research And Development Co. Ltd. | Cell membrane proteins, compositions containing them and procedure for their preparation |
IL69686A (en) * | 1983-09-11 | 1988-03-31 | Yeda Res & Dev | Compositions containing cell membrane proteins and process for their preparation |
US4645738A (en) * | 1983-09-30 | 1987-02-24 | Memorial Sloan-Kettering Institute Cancer Center | Method for differential diagnosis of T cell leukemias using monoclonal antibodies |
JPS60231699A (ja) * | 1983-11-09 | 1985-11-18 | Aichiken | 正常及び悪性ヒト造血器細胞に対するモノクローナル抗体 |
US4469630A (en) * | 1983-11-25 | 1984-09-04 | J. T. Baker Chemical Co. | Purification of monoclonal antibodies |
EP0146050B1 (en) | 1983-12-16 | 1990-07-04 | Denis M. Callewaert | Site selective plasminogen activator and method of making and using same |
US4965204A (en) * | 1984-02-06 | 1990-10-23 | The Johns Hopkins University | Human stem cells and monoclonal antibodies |
US5130144B1 (en) * | 1984-02-06 | 1995-08-15 | Univ Johns Hopkins | Human stem cells and monoclonal antibodies |
US4604235A (en) * | 1984-05-23 | 1986-08-05 | J. T. Baker Chemical Company | Purification of monoclonal antibodies |
EP0335426B1 (fr) * | 1984-09-14 | 1991-07-03 | Institut Pasteur | Composition et procédé pour protéger les lymphocytes T contre l'agent étiologique des lymphadénopathies et du syndrome d'immunisation acquise |
FR2570278B1 (fr) * | 1984-09-14 | 1987-02-13 | Pasteur Institut | Compositions et procede pour proteger les lymphocytes t contre l'agent etiologique des lymphadenopathies et du syndrome d'immunodepression acquise |
US4695459A (en) * | 1984-12-26 | 1987-09-22 | The Board Of Trustees Of Leland Stanford Junior University | Method of treating autoimmune diseases that are mediated by Leu3/CD4 phenotype T cells |
US4661446A (en) * | 1985-02-19 | 1987-04-28 | Dana-Farber Cancer Institute, Inc. | Monoclonal antibodies and method of immunizing therewith |
US5776093A (en) * | 1985-07-05 | 1998-07-07 | Immunomedics, Inc. | Method for imaging and treating organs and tissues |
DE3541033A1 (de) * | 1985-11-19 | 1987-05-21 | Boehringer Mannheim Gmbh | Verfahren zur quantifizierung von zellpopulationen bzw. subpopulationen sowie hierfuer geeignetes reagenz |
GB8608068D0 (en) * | 1986-04-02 | 1986-05-08 | Cobbold S P | Monoclonal antibodies |
CA1296622C (en) * | 1986-08-12 | 1992-03-03 | Jeffrey E. Anderson | Method and apparatus for automated assessment of the immunoregulatory status of the mononuclear leukocyte immune system |
US5601819A (en) * | 1988-08-11 | 1997-02-11 | The General Hospital Corporation | Bispecific antibodies for selective immune regulation and for selective immune cell binding |
US5068178A (en) * | 1988-12-06 | 1991-11-26 | Genetic Systems Corporation | Method of enhancing direct immunofluorescence staining of cells |
US7037496B2 (en) * | 1989-12-27 | 2006-05-02 | Centocor, Inc. | Chimeric immunoglobulin for CD4 receptors |
JPH0424999U (es) * | 1990-06-26 | 1992-02-28 | ||
GB9021677D0 (en) * | 1990-10-05 | 1990-11-21 | Xaar Ltd | Method of testing multi-channel array pulsed droplet deposition apparatus |
EP0512112B1 (en) * | 1990-11-27 | 1997-05-28 | Biogen, Inc. | Anti cd-4 antibodies blocking hiv-induced syncytia |
US5747265A (en) * | 1992-10-30 | 1998-05-05 | T Cell Diagnostics, Inc. | Method for measuring the amount of a cell-associated molecule |
US6193969B1 (en) * | 1993-06-03 | 2001-02-27 | Protherics Inc. | Antibody fragments in therapy |
US5961976A (en) * | 1996-06-03 | 1999-10-05 | United Biomedical, Inc. | Antibodies against a host cell antigen complex for pre- and post-exposure protection from infection by HIV |
ATE424410T1 (de) | 1996-08-30 | 2009-03-15 | Human Genome Sciences Inc | Interleukin-19. |
CA2310805A1 (en) | 1997-11-24 | 1999-06-03 | Johnson T. Wong | Methods for treatment of hiv or other infections using a t cell or viral activator and anti-retroviral combination therapy |
US6790796B2 (en) | 2001-10-05 | 2004-09-14 | Albany International Corp. | Nonwovens forming or conveying fabrics with enhanced surface roughness and texture |
JP2009530290A (ja) * | 2006-03-16 | 2009-08-27 | ジェネンテック・インコーポレーテッド | Cd4抗体を使用するループスの治療方法 |
US20080279848A1 (en) * | 2006-03-16 | 2008-11-13 | Genentech, Inc. | Methods of treating lupus using CD4 antibodies |
TW201016233A (en) * | 2008-07-15 | 2010-05-01 | Genentech Inc | Methods of treating autoimmune diseases using CD4 antibodies |
RU2762315C2 (ru) | 2014-09-16 | 2021-12-17 | Юнайтед Байофарма, Инк. | Лечение и функциональное излечение вич-инфекции моноклональными антителами к cd4, опосредующими конкурентное ингибирование входа вич |
SG11201901203PA (en) | 2016-08-13 | 2019-03-28 | United Biopharma Inc | Treatment and sustained virologic remission of hiv infection by antibodies to cd4 in haart stabilized patients |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4196265A (en) * | 1977-06-15 | 1980-04-01 | The Wistar Institute | Method of producing antibodies |
-
1979
- 1979-04-26 US US06/033,639 patent/US4381295A/en not_active Expired - Lifetime
-
1980
- 1980-04-01 CA CA000348981A patent/CA1170592A/en not_active Expired
- 1980-04-11 GR GR61667A patent/GR67298B/el unknown
- 1980-04-17 NZ NZ193470A patent/NZ193470A/en unknown
- 1980-04-24 AU AU57758/80A patent/AU544792B2/en not_active Expired
- 1980-04-24 PH PH23948A patent/PH16656A/en unknown
- 1980-04-24 JP JP5369080A patent/JPS55145616A/ja active Granted
- 1980-04-24 PT PT71148A patent/PT71148B/pt active IP Right Revival
- 1980-04-24 IL IL59919A patent/IL59919A/xx unknown
- 1980-04-25 NO NO801216A patent/NO159883C/no unknown
- 1980-04-25 EP EP80301356A patent/EP0018794B1/en not_active Expired
- 1980-04-25 ZA ZA00802524A patent/ZA802524B/xx unknown
- 1980-04-25 DE DE198080301356T patent/DE18794T1/de active Pending
- 1980-04-25 FI FI801343A patent/FI75363C/fi not_active IP Right Cessation
- 1980-04-25 ES ES490943A patent/ES8105151A1/es not_active Expired
- 1980-04-25 YU YU1148/80A patent/YU44957B/xx unknown
- 1980-04-25 AT AT80301356T patent/ATE14454T1/de not_active IP Right Cessation
- 1980-04-25 IE IE839/80A patent/IE50459B1/en not_active IP Right Cessation
- 1980-04-25 DK DK180580A patent/DK154087C/da active
- 1980-04-25 DE DE8080301356T patent/DE3070896D1/de not_active Expired
-
1986
- 1986-01-21 SG SG65/86A patent/SG6586G/en unknown
- 1986-04-03 HK HK237/86A patent/HK23786A/xx unknown
- 1986-12-30 MY MY513/86A patent/MY8600513A/xx unknown
-
1988
- 1988-04-26 JP JP63101497A patent/JPH0198477A/ja active Granted
Also Published As
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