ES541121A0 - Un procedimiento para preparar el plasmido plr 711 - Google Patents

Un procedimiento para preparar el plasmido plr 711

Info

Publication number
ES541121A0
ES541121A0 ES541121A ES541121A ES541121A0 ES 541121 A0 ES541121 A0 ES 541121A0 ES 541121 A ES541121 A ES 541121A ES 541121 A ES541121 A ES 541121A ES 541121 A0 ES541121 A0 ES 541121A0
Authority
ES
Spain
Prior art keywords
prochymosin
procedure
host cells
plasmids
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES541121A
Other languages
English (en)
Other versions
ES8705520A1 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of ES541121A0 publication Critical patent/ES541121A0/es
Publication of ES8705520A1 publication Critical patent/ES8705520A1/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6481Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/71Expression systems using regulatory sequences derived from the trp-operon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)
ES541121A 1984-03-09 1985-03-08 Un procedimiento para preparar el plasmido plr 711 Expired ES8705520A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59044901A JPS60188077A (ja) 1984-03-09 1984-03-09 仔牛プロキモシンcDΝAの全配列を有する新規な発現プラスミド

Publications (2)

Publication Number Publication Date
ES541121A0 true ES541121A0 (es) 1987-05-01
ES8705520A1 ES8705520A1 (es) 1987-05-01

Family

ID=12704373

Family Applications (4)

Application Number Title Priority Date Filing Date
ES541121A Expired ES8705520A1 (es) 1984-03-09 1985-03-08 Un procedimiento para preparar el plasmido plr 711
ES550868A Expired ES8802181A1 (es) 1984-03-09 1986-01-14 Un procedimiento para la produccion de proquimosina
ES550867A Expired ES8704205A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr714
ES550866A Expired ES8802183A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr712 o pcr713

Family Applications After (3)

Application Number Title Priority Date Filing Date
ES550868A Expired ES8802181A1 (es) 1984-03-09 1986-01-14 Un procedimiento para la produccion de proquimosina
ES550867A Expired ES8704205A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr714
ES550866A Expired ES8802183A1 (es) 1984-03-09 1986-01-14 Un procedimiento para preparar el plasmido pcr712 o pcr713

Country Status (11)

Country Link
US (1) US4757020A (es)
EP (1) EP0154350B1 (es)
JP (1) JPS60188077A (es)
AT (1) ATE74161T1 (es)
AU (1) AU594012B2 (es)
CA (1) CA1288072C (es)
DE (1) DE3585695D1 (es)
DK (1) DK107885A (es)
ES (4) ES8705520A1 (es)
IE (1) IE58820B1 (es)
IL (1) IL74537A (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3514113A1 (de) * 1985-04-19 1986-10-23 Hoechst Ag, 6230 Frankfurt Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression
IT1221765B (it) * 1986-05-07 1990-07-12 Eniricerche Spa Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli
US5045471A (en) * 1986-12-02 1991-09-03 The Regents Of The University Of California Cloned DNA for P450scc and expression thereof
JPH05505308A (ja) * 1990-03-13 1993-08-12 ハワイ・バイオテクノロジー・グループ・インコーポレイテツド アオパンカビ発現システム
US7482148B2 (en) * 2004-03-30 2009-01-27 Sudershan Biotech Ltd. Recombinant calf-chymosin and a process for producing the same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
IE53517B1 (en) * 1981-06-17 1988-12-07 Celltech Ltd Process for the production of a polypeptide
JPS589687A (ja) * 1981-06-17 1983-01-20 セルテク リミテッド ポリペプチドの生産方法
JPS58110600A (ja) * 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JPS59166086A (ja) * 1983-03-09 1984-09-19 Teruhiko Beppu 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法

Also Published As

Publication number Publication date
US4757020A (en) 1988-07-12
JPS60188077A (ja) 1985-09-25
ES8802181A1 (es) 1988-04-01
DK107885A (da) 1985-09-10
DE3585695D1 (de) 1992-04-30
JPH0515432B2 (es) 1993-03-01
ES550867A0 (es) 1987-03-16
AU3964085A (en) 1985-09-12
ES550868A0 (es) 1988-04-01
DK107885D0 (da) 1985-03-08
ES8704205A1 (es) 1987-03-16
EP0154350A3 (en) 1987-04-22
ES550866A0 (es) 1988-04-01
AU594012B2 (en) 1990-03-01
ES8802183A1 (es) 1988-04-01
CA1288072C (en) 1991-08-27
IE850606L (en) 1985-09-09
ES8705520A1 (es) 1987-05-01
EP0154350A2 (en) 1985-09-11
IL74537A (en) 1992-03-29
IE58820B1 (en) 1993-11-17
IL74537A0 (en) 1985-06-30
EP0154350B1 (en) 1992-03-25
ATE74161T1 (de) 1992-04-15

Similar Documents

Publication Publication Date Title
ES2072893T3 (es) Endoproteasa kex2 y procedimiento para su produccion.
ATE88501T1 (de) Rekombinante dns-expressionsvektoren.
KR870700074A (ko) Fsh
DE3477810D1 (en) Polypeptide and protein products, and processes for their production and use
ES9000011A1 (es) Procedimiento para transformar un cultivo de celulas de modo que produzca activador de plasminogeno de tejido (t-pa) humano
NO176921C (no) Genteknologisk fremgangsmåte for fremstilling av et tPA-lignende protein, samt vertscelle som er i stand til å uttrykke et slikt protein
IL114820A0 (en) Method for selecting high-expressing host cells
DK470184A (da) Cdna-kloner, der koder for polypeptider, som udviser vaekstfaktoraktivitet med hensyn til mangeartede celler og/eller mastceller
ES518157A0 (es) Un procedimiento para preparar una enzima alfa-amilasa termoestable.
KR880700855A (ko) 진핵생물 단백질을 발현시키는 개선된 방법
ES541121A0 (es) Un procedimiento para preparar el plasmido plr 711
ATE60933T1 (de) Fuer kaninchen-tnf kodierende dns, vektor mit dieser dns darin inseriert, mit diesem vektor transformierter wirt, kaninchen-tnf-polypeptid und verfahren zur herstellung desselben.
KR880001816A (ko) 변이 인간 프로유로키나제 및 이의 제조방법
ES8605813A1 (es) Procedimiento de tecnologia genetica para preparar interleu-quina-2 de humano
KR860000383A (ko) 약물의 유전공학적 제조방법
EP0292879A3 (en) An episomal vector for the expression of selected dna fragments coding for polypeptides in mammalian cells and methods for the production thereof
AUPM772494A0 (en) Improvements in production of proteins in host cells
AU7802587A (en) High level inducible expression of heterologous genes
DE3380878D1 (en) Method of heterologous cloning of gene in bacillus microorganism
ES2003085A6 (es) Procedimiento para inducir de una forma controlada la expresion de genes eucarioticos de interes en celulas hospedantes eucarioticas apropiadas
IL68797A0 (en) Cloned bovine calf chymosin and calf prochymosin genes,their preparation,plasmids containing them and microorganisms transformed by those plasmids
JPS6485920A (es)
PT83247B (pt) Processo para a preparacao de hidroxialquil-fenoxi-propan-2-olaminas uteis como beta-bloqueadores oculosselectivos
ES548457A0 (es) Procedimiento para la preparacion de penicilin-g-amidasa por expresion de la enzima en el seno de un microorganismo.
JPS642584A (en) Dna and plasmid, and microorganism containing the same

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20020401