ES449739A1 - Chromogenic thrombin substrates - Google Patents
Chromogenic thrombin substratesInfo
- Publication number
- ES449739A1 ES449739A1 ES449739A ES449739A ES449739A1 ES 449739 A1 ES449739 A1 ES 449739A1 ES 449739 A ES449739 A ES 449739A ES 449739 A ES449739 A ES 449739A ES 449739 A1 ES449739 A1 ES 449739A1
- Authority
- ES
- Spain
- Prior art keywords
- substrate
- enzymes
- formula
- amount
- thrombin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/06—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
- C07K1/061—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups
- C07K1/064—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups for omega-amino or -guanidino functions
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0802—Tripeptides with the first amino acid being neutral
- C07K5/0812—Tripeptides with the first amino acid being neutral and aromatic or cycloaliphatic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/30—Naphthyl amides, e.g. beta-NA, 2-NA, 4-methoxy-beta-naphthylamine, i.e. 4MNA
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Neurosurgery (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
To determine thrombin or enzymes which cleave the substrates in the same way as thrombin, the material in which the enzymes are to be determined is brought into contact with a novel substrate of the formula I < IMAGE > In the substrate, R2 is a chromogenic radical which is eliminated by enzymatic hydrolysis in the form of the corresponding amine which differs in its absorption spectrum from the substrate of the formula I so that quantitative determination of the enzyme present in the material is possible because the amount of amine which is formed is proportional to the amount of enzyme which is present. In the substrate, the substituents have the meaning stated in Claim 1, and the optionally substituted 1-amino-2-phenylpropionic acid in the peptide chain must be in the D form so that attack of aminopeptidases on the substrate, which would interfere with the determination, is prevented.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE7507975-6A SE407405B (en) | 1975-07-11 | 1975-07-11 | NEW CHROMOGENATE THROMBIN SUBSTRATE |
Publications (1)
Publication Number | Publication Date |
---|---|
ES449739A1 true ES449739A1 (en) | 1978-01-16 |
Family
ID=20325116
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES449739A Expired ES449739A1 (en) | 1975-07-11 | 1976-07-09 | Chromogenic thrombin substrates |
Country Status (22)
Country | Link |
---|---|
JP (1) | JPS5224590A (en) |
AT (1) | AT348686B (en) |
AU (1) | AU497547B2 (en) |
BE (1) | BE843970A (en) |
CA (1) | CA1068261A (en) |
CH (1) | CH622287A5 (en) |
CS (1) | CS196314B2 (en) |
DD (1) | DD128691A5 (en) |
DE (1) | DE2629195C3 (en) |
DK (1) | DK145799C (en) |
ES (1) | ES449739A1 (en) |
FI (1) | FI56525C (en) |
FR (1) | FR2317280A1 (en) |
GB (1) | GB1510926A (en) |
IL (1) | IL49829A (en) |
IT (1) | IT1062605B (en) |
NL (1) | NL188355C (en) |
NO (1) | NO142576C (en) |
PL (1) | PL103003B1 (en) |
SE (1) | SE407405B (en) |
SU (1) | SU719492A3 (en) |
ZA (1) | ZA763586B (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5255593A (en) * | 1975-10-30 | 1977-05-07 | Ajinomoto Kk | Measuring method of enzyme activity |
DE2757992A1 (en) * | 1977-12-24 | 1979-06-28 | Boehringer Mannheim Gmbh | METHOD FOR PROTHROMBIN DETERMINATION |
DE2812943C3 (en) * | 1978-03-23 | 1981-05-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | Method and reagent for determining the biological activity of heparin in plasma |
US4289498A (en) * | 1979-01-08 | 1981-09-15 | Ortho Diagnostics, Inc. | One-stage prothrombin assay and compositions useful therein |
EP0018002B1 (en) * | 1979-04-24 | 1983-02-09 | Marcel Jozefonvicz | Process for the determination of proteases and antiproteases |
FR2471410A2 (en) * | 1979-12-17 | 1981-06-19 | Jozefonvicz Marcel | Electrochemical protease or anti-protease determn. - by amperometric determn. of amine released from peptide amide substrate |
DE3005540A1 (en) * | 1980-02-14 | 1981-08-20 | Boehringer Mannheim Gmbh, 6800 Mannheim | METHOD AND REAGENT FOR DETERMINING THE BIOLOGICAL ACTIVITY OF HEPARIN IN PLASMA |
DK155051C (en) * | 1980-05-06 | 1989-07-03 | Pentapharm Ag | TRIPEPTIDE DERIVATIVES AND PROCEDURES FOR QUANTITATIVE ANALYSIS OF PROTEOLYTIC ENZYMES UNDER USE thereof |
JPS5856695A (en) * | 1981-09-28 | 1983-04-04 | Nitto Boseki Co Ltd | Novel substrate for assay of thrombin |
DE3211254A1 (en) * | 1982-03-26 | 1983-09-29 | Boehringer Mannheim Gmbh, 6800 Mannheim | METHOD FOR DETECTING THE PRESENCE OF AN ALLERGY AND FOR SPECIFIC DETECTING THE ALLERGY RESPONSIBLE FOR THE ALLERGY |
DK201084A (en) * | 1983-04-28 | 1984-10-29 | Kimberly Clark Co | PROCEDURE FOR DETERMINING CATHEPSIN B IN THE PRESENCE OF OTHER PROTEOLYTIC ENZYMES AND RELATIONSHIPS FOR USING THE PROCEDURE |
EP1889919A4 (en) | 2005-05-20 | 2009-04-15 | Mitsubishi Kagaku Iatron Inc | Method of analyzing enzyme |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE380258B (en) * | 1972-05-02 | 1975-11-03 | Bofors Ab | NEW DIAGNOSTIC OPERATING SUBSIDIES WITH HIGH SPECIFICITY FOR TRYPSIN AND OTHER PEPTIDYL-PEPTIDE HYDROLASES OF THE PEPTIDYL TYPE |
SE380257B (en) * | 1972-05-02 | 1975-11-03 | Bofors Ab | NEW DIAGNOSTIC OPERATING SUBSTRATES WITH HIGH SPECIFICITY FOR THROMBIN AND OTHER PROTEOLYTIC ENZYMES OF THE PEPTIDYL-PEPTIDE HYDROLASES |
-
1975
- 1975-07-11 SE SE7507975-6A patent/SE407405B/en not_active IP Right Cessation
-
1976
- 1976-06-16 ZA ZA763586A patent/ZA763586B/en unknown
- 1976-06-17 IL IL49829A patent/IL49829A/en unknown
- 1976-06-21 GB GB25580/76A patent/GB1510926A/en not_active Expired
- 1976-06-25 AU AU15305/76A patent/AU497547B2/en not_active Expired
- 1976-06-25 AT AT463976A patent/AT348686B/en not_active IP Right Cessation
- 1976-06-29 DE DE2629195A patent/DE2629195C3/en not_active Expired
- 1976-06-29 NL NLAANVRAGE7607104,A patent/NL188355C/en not_active IP Right Cessation
- 1976-07-06 CA CA256,417A patent/CA1068261A/en not_active Expired
- 1976-07-08 CS CS764544A patent/CS196314B2/en unknown
- 1976-07-09 BE BE168774A patent/BE843970A/en not_active IP Right Cessation
- 1976-07-09 CH CH887476A patent/CH622287A5/en not_active IP Right Cessation
- 1976-07-09 ES ES449739A patent/ES449739A1/en not_active Expired
- 1976-07-09 PL PL1976191068A patent/PL103003B1/en unknown
- 1976-07-09 NO NO762407A patent/NO142576C/en unknown
- 1976-07-09 SU SU762379659A patent/SU719492A3/en active
- 1976-07-09 IT IT50364/76A patent/IT1062605B/en active
- 1976-07-09 DK DK312676A patent/DK145799C/en not_active IP Right Cessation
- 1976-07-09 FR FR7621196A patent/FR2317280A1/en active Granted
- 1976-07-09 DD DD7600193785A patent/DD128691A5/en unknown
- 1976-07-09 FI FI762010A patent/FI56525C/en not_active IP Right Cessation
- 1976-07-10 JP JP51082442A patent/JPS5224590A/en active Granted
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