ES3000665T3 - High amylose wheat - ii - Google Patents
High amylose wheat - ii Download PDFInfo
- Publication number
- ES3000665T3 ES3000665T3 ES12846192T ES12846192T ES3000665T3 ES 3000665 T3 ES3000665 T3 ES 3000665T3 ES 12846192 T ES12846192 T ES 12846192T ES 12846192 T ES12846192 T ES 12846192T ES 3000665 T3 ES3000665 T3 ES 3000665T3
- Authority
- ES
- Spain
- Prior art keywords
- sbeiia
- starch
- grain
- gene
- wheat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920000856 Amylose Polymers 0.000 title claims abstract description 263
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 256
- 241000209140 Triticum Species 0.000 title claims abstract description 13
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 589
- 229920002472 Starch Polymers 0.000 claims abstract description 387
- 235000019698 starch Nutrition 0.000 claims abstract description 366
- 239000008107 starch Substances 0.000 claims abstract description 360
- 244000098338 Triticum aestivum Species 0.000 claims abstract description 337
- 235000013339 cereals Nutrition 0.000 claims abstract description 333
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 187
- 230000000694 effects Effects 0.000 claims abstract description 154
- 230000035772 mutation Effects 0.000 claims abstract description 146
- 108700028369 Alleles Proteins 0.000 claims abstract description 139
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 46
- 241000196324 Embryophyta Species 0.000 claims description 240
- 238000000034 method Methods 0.000 claims description 125
- 239000008187 granular material Substances 0.000 claims description 106
- 230000014509 gene expression Effects 0.000 claims description 81
- 238000012217 deletion Methods 0.000 claims description 70
- 230000037430 deletion Effects 0.000 claims description 70
- 230000002829 reductive effect Effects 0.000 claims description 66
- 108090000344 1,4-alpha-Glucan Branching Enzyme Proteins 0.000 claims description 61
- 102000003925 1,4-alpha-Glucan Branching Enzyme Human genes 0.000 claims description 61
- 229920000945 Amylopectin Polymers 0.000 claims description 51
- 230000009261 transgenic effect Effects 0.000 claims description 50
- 235000013305 food Nutrition 0.000 claims description 48
- 229920000294 Resistant starch Polymers 0.000 claims description 39
- 235000021254 resistant starch Nutrition 0.000 claims description 39
- 230000001965 increasing effect Effects 0.000 claims description 36
- 102000039446 nucleic acids Human genes 0.000 claims description 31
- 108020004707 nucleic acids Proteins 0.000 claims description 31
- 150000007523 nucleic acids Chemical class 0.000 claims description 31
- 235000013312 flour Nutrition 0.000 claims description 29
- 238000001262 western blot Methods 0.000 claims description 22
- 229940100445 wheat starch Drugs 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 19
- 235000013361 beverage Nutrition 0.000 claims description 18
- 238000009826 distribution Methods 0.000 claims description 18
- 230000004777 loss-of-function mutation Effects 0.000 claims description 18
- 230000035784 germination Effects 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 17
- 238000004519 manufacturing process Methods 0.000 claims description 16
- 239000004615 ingredient Substances 0.000 claims description 13
- 235000012054 meals Nutrition 0.000 claims description 11
- 230000008961 swelling Effects 0.000 claims description 11
- 238000012545 processing Methods 0.000 claims description 10
- 230000002641 glycemic effect Effects 0.000 claims description 9
- 235000012041 food component Nutrition 0.000 claims description 6
- 239000005417 food ingredient Substances 0.000 claims description 6
- 238000003801 milling Methods 0.000 claims description 5
- 238000003306 harvesting Methods 0.000 claims description 4
- 230000003111 delayed effect Effects 0.000 claims description 3
- 101100504555 Pisum sativum SBEII gene Proteins 0.000 claims 1
- 238000013519 translation Methods 0.000 abstract description 16
- 108010054045 starch-branching enzyme IIb Proteins 0.000 description 190
- 235000018102 proteins Nutrition 0.000 description 168
- 239000002773 nucleotide Substances 0.000 description 130
- 125000003729 nucleotide group Chemical group 0.000 description 129
- 108010031092 starch-branching enzyme II Proteins 0.000 description 84
- 230000000875 corresponding effect Effects 0.000 description 74
- 125000003275 alpha amino acid group Chemical group 0.000 description 63
- 108020004414 DNA Proteins 0.000 description 61
- 239000000047 product Substances 0.000 description 57
- 210000004027 cell Anatomy 0.000 description 55
- 108090000765 processed proteins & peptides Proteins 0.000 description 53
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 47
- 239000012634 fragment Substances 0.000 description 47
- 102000040430 polynucleotide Human genes 0.000 description 46
- 108091033319 polynucleotide Proteins 0.000 description 46
- 239000002157 polynucleotide Substances 0.000 description 46
- 102000004196 processed proteins & peptides Human genes 0.000 description 45
- 229940088598 enzyme Drugs 0.000 description 44
- 102000004190 Enzymes Human genes 0.000 description 43
- 108090000790 Enzymes Proteins 0.000 description 43
- 229920001184 polypeptide Polymers 0.000 description 43
- 238000004458 analytical method Methods 0.000 description 40
- 238000006467 substitution reaction Methods 0.000 description 40
- 238000003752 polymerase chain reaction Methods 0.000 description 38
- 230000002068 genetic effect Effects 0.000 description 32
- 238000003556 assay Methods 0.000 description 31
- 101710124145 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic Proteins 0.000 description 30
- 239000000499 gel Substances 0.000 description 29
- 239000000523 sample Substances 0.000 description 29
- 210000001519 tissue Anatomy 0.000 description 29
- 150000001413 amino acids Chemical class 0.000 description 28
- 108010029485 Protein Isoforms Proteins 0.000 description 26
- 102000001708 Protein Isoforms Human genes 0.000 description 26
- 108700019146 Transgenes Proteins 0.000 description 26
- 230000000295 complement effect Effects 0.000 description 25
- 230000009467 reduction Effects 0.000 description 25
- 238000004497 NIR spectroscopy Methods 0.000 description 23
- 108700024394 Exon Proteins 0.000 description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 21
- 238000003780 insertion Methods 0.000 description 21
- 230000037431 insertion Effects 0.000 description 21
- 239000003550 marker Substances 0.000 description 21
- 239000002243 precursor Substances 0.000 description 21
- 230000015572 biosynthetic process Effects 0.000 description 19
- 239000002299 complementary DNA Substances 0.000 description 19
- 108091027967 Small hairpin RNA Proteins 0.000 description 18
- 210000000349 chromosome Anatomy 0.000 description 18
- 231100000350 mutagenesis Toxicity 0.000 description 18
- 230000036961 partial effect Effects 0.000 description 18
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 17
- 239000000463 material Substances 0.000 description 17
- 108020004999 messenger RNA Proteins 0.000 description 17
- 230000004048 modification Effects 0.000 description 17
- 238000012986 modification Methods 0.000 description 17
- 101150110449 SBEII gene Proteins 0.000 description 16
- 230000000692 anti-sense effect Effects 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 16
- 210000002257 embryonic structure Anatomy 0.000 description 16
- 238000002703 mutagenesis Methods 0.000 description 16
- 230000014616 translation Effects 0.000 description 16
- 238000011161 development Methods 0.000 description 15
- 230000018109 developmental process Effects 0.000 description 15
- 241000894007 species Species 0.000 description 15
- 241001522110 Aegilops tauschii Species 0.000 description 14
- 241000589158 Agrobacterium Species 0.000 description 14
- 108091034117 Oligonucleotide Proteins 0.000 description 14
- 240000008042 Zea mays Species 0.000 description 14
- 238000001514 detection method Methods 0.000 description 14
- 238000001502 gel electrophoresis Methods 0.000 description 14
- 108091070501 miRNA Proteins 0.000 description 14
- 239000002679 microRNA Substances 0.000 description 14
- 102000054765 polymorphisms of proteins Human genes 0.000 description 14
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 13
- 108091028043 Nucleic acid sequence Proteins 0.000 description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 13
- 230000008859 change Effects 0.000 description 13
- 239000004382 Amylase Substances 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 12
- 230000003321 amplification Effects 0.000 description 12
- 230000029087 digestion Effects 0.000 description 12
- 229910052740 iodine Inorganic materials 0.000 description 12
- 238000000386 microscopy Methods 0.000 description 12
- 238000003199 nucleic acid amplification method Methods 0.000 description 12
- 230000001603 reducing effect Effects 0.000 description 12
- 230000009466 transformation Effects 0.000 description 12
- 206010020649 Hyperkeratosis Diseases 0.000 description 11
- -1 SBEIIa Proteins 0.000 description 11
- 238000002835 absorbance Methods 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 230000001105 regulatory effect Effects 0.000 description 11
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 10
- 240000005979 Hordeum vulgare Species 0.000 description 10
- 235000007340 Hordeum vulgare Nutrition 0.000 description 10
- 150000001720 carbohydrates Chemical class 0.000 description 10
- 239000008103 glucose Substances 0.000 description 10
- 230000012010 growth Effects 0.000 description 10
- 239000011630 iodine Substances 0.000 description 10
- 108091008146 restriction endonucleases Proteins 0.000 description 10
- 238000002864 sequence alignment Methods 0.000 description 10
- 239000002924 silencing RNA Substances 0.000 description 10
- 238000003786 synthesis reaction Methods 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 238000012546 transfer Methods 0.000 description 10
- 235000011844 whole wheat flour Nutrition 0.000 description 10
- 241000736839 Chara Species 0.000 description 9
- 229920001503 Glucan Polymers 0.000 description 9
- 108091092195 Intron Proteins 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 9
- 235000014633 carbohydrates Nutrition 0.000 description 9
- 230000030279 gene silencing Effects 0.000 description 9
- 239000013598 vector Substances 0.000 description 9
- 108010065511 Amylases Proteins 0.000 description 8
- 102000013142 Amylases Human genes 0.000 description 8
- 238000012357 Gap analysis Methods 0.000 description 8
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 8
- 235000019418 amylase Nutrition 0.000 description 8
- 230000003247 decreasing effect Effects 0.000 description 8
- 238000001962 electrophoresis Methods 0.000 description 8
- 239000000284 extract Substances 0.000 description 8
- 150000002632 lipids Chemical class 0.000 description 8
- 235000009973 maize Nutrition 0.000 description 8
- 229920000642 polymer Polymers 0.000 description 8
- 230000002028 premature Effects 0.000 description 8
- 238000003860 storage Methods 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 7
- 240000008620 Fagopyrum esculentum Species 0.000 description 7
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 7
- 108091092878 Microsatellite Proteins 0.000 description 7
- 240000007594 Oryza sativa Species 0.000 description 7
- 235000007164 Oryza sativa Nutrition 0.000 description 7
- 108010039811 Starch synthase Proteins 0.000 description 7
- 208000035199 Tetraploidy Diseases 0.000 description 7
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 230000004075 alteration Effects 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 238000012226 gene silencing method Methods 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 210000000056 organ Anatomy 0.000 description 7
- 210000002706 plastid Anatomy 0.000 description 7
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Substances [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 235000009566 rice Nutrition 0.000 description 7
- 239000004055 small Interfering RNA Substances 0.000 description 7
- 238000001228 spectrum Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 238000013518 transcription Methods 0.000 description 7
- 230000035897 transcription Effects 0.000 description 7
- 241000209148 Aegilops speltoides Species 0.000 description 6
- 229920001353 Dextrin Polymers 0.000 description 6
- 239000004375 Dextrin Substances 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 108700011259 MicroRNAs Proteins 0.000 description 6
- 238000002944 PCR assay Methods 0.000 description 6
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 6
- 108020004459 Small interfering RNA Proteins 0.000 description 6
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
- 230000009418 agronomic effect Effects 0.000 description 6
- 235000019425 dextrin Nutrition 0.000 description 6
- 235000013325 dietary fiber Nutrition 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 230000035558 fertility Effects 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 230000009368 gene silencing by RNA Effects 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 6
- 230000007413 intestinal health Effects 0.000 description 6
- 238000013507 mapping Methods 0.000 description 6
- 229920002401 polyacrylamide Polymers 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- 238000001542 size-exclusion chromatography Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 230000037436 splice-site mutation Effects 0.000 description 6
- WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 description 5
- WFPZSXYXPSUOPY-UHFFFAOYSA-N ADP-mannose Natural products C1=NC=2C(N)=NC=NC=2N1C(C(C1O)O)OC1COP(O)(=O)OP(O)(=O)OC1OC(CO)C(O)C(O)C1O WFPZSXYXPSUOPY-UHFFFAOYSA-N 0.000 description 5
- 108700023224 Glucose-1-phosphate adenylyltransferases Proteins 0.000 description 5
- 241000282412 Homo Species 0.000 description 5
- 108700026244 Open Reading Frames Proteins 0.000 description 5
- 238000012408 PCR amplification Methods 0.000 description 5
- 238000010222 PCR analysis Methods 0.000 description 5
- 101001135788 Pinus taeda (+)-alpha-pinene synthase, chloroplastic Proteins 0.000 description 5
- 244000061456 Solanum tuberosum Species 0.000 description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 235000008429 bread Nutrition 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000000470 constituent Substances 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 230000002550 fecal effect Effects 0.000 description 5
- 238000005194 fractionation Methods 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 238000005213 imbibition Methods 0.000 description 5
- 238000003119 immunoblot Methods 0.000 description 5
- 238000010348 incorporation Methods 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 210000002429 large intestine Anatomy 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000008363 phosphate buffer Substances 0.000 description 5
- 230000004952 protein activity Effects 0.000 description 5
- 230000005855 radiation Effects 0.000 description 5
- 238000003753 real-time PCR Methods 0.000 description 5
- 238000005204 segregation Methods 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 5
- 229920001685 Amylomaize Polymers 0.000 description 4
- 108091026890 Coding region Proteins 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 108091027305 Heteroduplex Proteins 0.000 description 4
- 229920000881 Modified starch Polymers 0.000 description 4
- 238000001190 Q-PCR Methods 0.000 description 4
- HXXFSFRBOHSIMQ-VFUOTHLCSA-N alpha-D-glucose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-VFUOTHLCSA-N 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 229950010772 glucose-1-phosphate Drugs 0.000 description 4
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 4
- 230000002363 herbicidal effect Effects 0.000 description 4
- 239000004009 herbicide Substances 0.000 description 4
- 230000001900 immune effect Effects 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 238000010849 ion bombardment Methods 0.000 description 4
- 230000008774 maternal effect Effects 0.000 description 4
- 235000019426 modified starch Nutrition 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 230000000291 postprandial effect Effects 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 230000008929 regeneration Effects 0.000 description 4
- 238000011069 regeneration method Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 210000000813 small intestine Anatomy 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- 238000003260 vortexing Methods 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
- 229920002527 Glycogen Polymers 0.000 description 3
- 102000004877 Insulin Human genes 0.000 description 3
- 108090001061 Insulin Proteins 0.000 description 3
- 108091027974 Mature messenger RNA Proteins 0.000 description 3
- 108020004485 Nonsense Codon Proteins 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 108010073135 Phosphorylases Proteins 0.000 description 3
- 102000009097 Phosphorylases Human genes 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 108020005038 Terminator Codon Proteins 0.000 description 3
- 241000209143 Triticum turgidum subsp. durum Species 0.000 description 3
- 241000209147 Triticum urartu Species 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 230000009102 absorption Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000013043 chemical agent Substances 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 238000000113 differential scanning calorimetry Methods 0.000 description 3
- 230000003828 downregulation Effects 0.000 description 3
- 230000000408 embryogenic effect Effects 0.000 description 3
- 238000007824 enzymatic assay Methods 0.000 description 3
- 231100000221 frame shift mutation induction Toxicity 0.000 description 3
- 230000037433 frameshift Effects 0.000 description 3
- 210000003736 gastrointestinal content Anatomy 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Natural products O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 229940096919 glycogen Drugs 0.000 description 3
- 230000007407 health benefit Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 229940125396 insulin Drugs 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000005304 joining Methods 0.000 description 3
- 230000002475 laxative effect Effects 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 230000010034 metabolic health Effects 0.000 description 3
- 231100000219 mutagenic Toxicity 0.000 description 3
- 239000003471 mutagenic agent Substances 0.000 description 3
- 230000003505 mutagenic effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008775 paternal effect Effects 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 210000001938 protoplast Anatomy 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 235000021391 short chain fatty acids Nutrition 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 101150073246 AGL1 gene Proteins 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 241000272517 Anseriformes Species 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- 101710115643 Cathelicidin-1 Proteins 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- 241000282326 Felis catus Species 0.000 description 2
- 239000004606 Fillers/Extenders Substances 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 2
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 2
- 239000004368 Modified starch Substances 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 241000286209 Phasianidae Species 0.000 description 2
- 108010065084 Phosphorylase a Proteins 0.000 description 2
- 101710152403 Prosaposin Proteins 0.000 description 2
- 108020005067 RNA Splice Sites Proteins 0.000 description 2
- 101150113575 SBEI gene Proteins 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 108700005078 Synthetic Genes Proteins 0.000 description 2
- 108010073062 Transcription Activator-Like Effectors Proteins 0.000 description 2
- 241000209146 Triticum sp. Species 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 230000036579 abiotic stress Effects 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- OJOBTAOGJIWAGB-UHFFFAOYSA-N acetosyringone Chemical compound COC1=CC(C(C)=O)=CC(OC)=C1O OJOBTAOGJIWAGB-UHFFFAOYSA-N 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 238000002299 affinity electrophoresis Methods 0.000 description 2
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000010165 autogamy Effects 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 239000003613 bile acid Substances 0.000 description 2
- 239000003124 biologic agent Substances 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000036996 cardiovascular health Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000004464 cereal grain Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000002962 chemical mutagen Substances 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 210000001072 colon Anatomy 0.000 description 2
- 238000004440 column chromatography Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 229940109239 creatinine Drugs 0.000 description 2
- 238000009402 cross-breeding Methods 0.000 description 2
- 238000002790 cross-validation Methods 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
- 230000001627 detrimental effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000016097 disease of metabolism Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 231100000502 fertility decrease Toxicity 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 230000007614 genetic variation Effects 0.000 description 2
- 238000003205 genotyping method Methods 0.000 description 2
- 108010050792 glutenin Proteins 0.000 description 2
- 235000011868 grain product Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 238000010884 ion-beam technique Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000021121 meiosis Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 208000030159 metabolic disease Diseases 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 description 2
- 230000026731 phosphorylation Effects 0.000 description 2
- 238000006366 phosphorylation reaction Methods 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 230000003405 preventing effect Effects 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000010188 recombinant method Methods 0.000 description 2
- 230000000306 recurrent effect Effects 0.000 description 2
- 238000007430 reference method Methods 0.000 description 2
- 230000008263 repair mechanism Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000004626 scanning electron microscopy Methods 0.000 description 2
- 230000008117 seed development Effects 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 150000004666 short chain fatty acids Chemical class 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000005063 solubilization Methods 0.000 description 2
- 230000007928 solubilization Effects 0.000 description 2
- 108010023236 starch synthase II Proteins 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 229940027257 timentin Drugs 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 238000001238 wet grinding Methods 0.000 description 2
- 235000020985 whole grains Nutrition 0.000 description 2
- HBOMLICNUCNMMY-KJFJCRTCSA-N 1-[(4s,5s)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1C1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-KJFJCRTCSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical compound [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 description 1
- 108020005345 3' Untranslated Regions Proteins 0.000 description 1
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 1
- WRDABNWSWOHGMS-UHFFFAOYSA-N AEBSF hydrochloride Chemical compound Cl.NCCC1=CC=C(S(F)(=O)=O)C=C1 WRDABNWSWOHGMS-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000209758 Aegilops Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 101100111164 Arabidopsis thaliana BAC2 gene Proteins 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 208000031639 Chromosome Deletion Diseases 0.000 description 1
- 241000320892 Clerodendrum phlomidis Species 0.000 description 1
- 241000287937 Colinus Species 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108010062580 Concanavalin A Proteins 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- NBSCHQHZLSJFNQ-QTVWNMPRSA-N D-Mannose-6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@@H]1O NBSCHQHZLSJFNQ-QTVWNMPRSA-N 0.000 description 1
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102100023387 Endoribonuclease Dicer Human genes 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 108091092566 Extrachromosomal DNA Proteins 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 102100040004 Gamma-glutamylcyclotransferase Human genes 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 239000005980 Gibberellic acid Substances 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 108010073032 Grain Proteins Proteins 0.000 description 1
- 240000004153 Hibiscus sabdariffa Species 0.000 description 1
- 101000907904 Homo sapiens Endoribonuclease Dicer Proteins 0.000 description 1
- 101000886680 Homo sapiens Gamma-glutamylcyclotransferase Proteins 0.000 description 1
- 238000009015 Human TaqMan MicroRNA Assay kit Methods 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 108010028688 Isoamylase Proteins 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- 101100288095 Klebsiella pneumoniae neo gene Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 238000008214 LDL Cholesterol Methods 0.000 description 1
- 239000007987 MES buffer Substances 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 101100257820 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) ssp-1 gene Proteins 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- 108020005093 RNA Precursors Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 108010057163 Ribonuclease III Proteins 0.000 description 1
- 102000003661 Ribonuclease III Human genes 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- 241000228160 Secale cereale x Triticum aestivum Species 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108010052160 Site-specific recombinase Proteins 0.000 description 1
- 241000187391 Streptomyces hygroscopicus Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 108010020764 Transposases Proteins 0.000 description 1
- 102000008579 Transposases Human genes 0.000 description 1
- 208000026487 Triploidy Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 235000019714 Triticale Nutrition 0.000 description 1
- 241000973013 Triticum aestivum var. vavilovii Species 0.000 description 1
- 244000228852 Triticum baeoticum Species 0.000 description 1
- 240000006716 Triticum compactum Species 0.000 description 1
- 240000008056 Triticum dicoccoides Species 0.000 description 1
- 240000000359 Triticum dicoccon Species 0.000 description 1
- 235000007264 Triticum durum Nutrition 0.000 description 1
- 244000102426 Triticum macha Species 0.000 description 1
- 240000000581 Triticum monococcum Species 0.000 description 1
- 244000085553 Triticum polonicum Species 0.000 description 1
- 240000003834 Triticum spelta Species 0.000 description 1
- 240000004176 Triticum sphaerococcum Species 0.000 description 1
- 240000002805 Triticum turgidum Species 0.000 description 1
- 235000007247 Triticum turgidum Nutrition 0.000 description 1
- 244000152061 Triticum turgidum ssp durum Species 0.000 description 1
- 235000003404 Triticum turgidum ssp. durum Nutrition 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 108010050181 aleurone Proteins 0.000 description 1
- 238000007844 allele-specific PCR Methods 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 1
- 238000003928 amperometric titration Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- VGPYEHKOIGNJKV-UHFFFAOYSA-N asulam Chemical compound COC(=O)NS(=O)(=O)C1=CC=C(N)C=C1 VGPYEHKOIGNJKV-UHFFFAOYSA-N 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 150000001540 azides Chemical class 0.000 description 1
- 238000000498 ball milling Methods 0.000 description 1
- 229940098396 barley grain Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010019077 beta-Amylase Proteins 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 235000021257 carbohydrate digestion Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000007073 chemical hydrolysis Effects 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000000326 densiometry Methods 0.000 description 1
- 238000010217 densitometric analysis Methods 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000018823 dietary intake Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 1
- 229960005156 digoxin Drugs 0.000 description 1
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000009837 dry grinding Methods 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000021759 endosperm development Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021197 fiber intake Nutrition 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000004545 gene duplication Effects 0.000 description 1
- 238000003197 gene knockdown Methods 0.000 description 1
- 230000037440 gene silencing effect Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000004192 high performance gel permeation chromatography Methods 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 101150047832 hpt gene Proteins 0.000 description 1
- 235000006486 human diet Nutrition 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000012770 industrial material Substances 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 101150026430 manA gene Proteins 0.000 description 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 210000000473 mesophyll cell Anatomy 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 229910052754 neon Inorganic materials 0.000 description 1
- GKAOGPIIYCISHV-UHFFFAOYSA-N neon atom Chemical compound [Ne] GKAOGPIIYCISHV-UHFFFAOYSA-N 0.000 description 1
- 238000005312 nonlinear dynamic Methods 0.000 description 1
- 230000037434 nonsense mutation Effects 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 108010007425 oligomycin sensitivity conferring protein Proteins 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000036470 plasma concentration Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 230000035040 seed growth Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000003998 size exclusion chromatography high performance liquid chromatography Methods 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- UGTZMIPZNRIWHX-UHFFFAOYSA-K sodium trimetaphosphate Chemical compound [Na+].[Na+].[Na+].[O-]P1(=O)OP([O-])(=O)OP([O-])(=O)O1 UGTZMIPZNRIWHX-UHFFFAOYSA-K 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000030118 somatic embryogenesis Effects 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 235000012794 white bread Nutrition 0.000 description 1
- 235000011845 white flour Nutrition 0.000 description 1
- 238000002424 x-ray crystallography Methods 0.000 description 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1
- WHGYBXFWUBPSRW-FEYSZYNQSA-N β-dextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)C(O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FEYSZYNQSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4678—Triticum sp. [wheat]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/197—Treatment of whole grains not provided for in groups A23L7/117 - A23L7/196
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C9/00—Other milling methods or mills specially adapted for grain
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/04—Extraction or purification
- C08B30/042—Extraction or purification from cereals or grains
- C08B30/046—Extraction or purification from cereals or grains from wheat
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01018—1,4-Alpha-glucan branching enzyme (2.4.1.18), i.e. glucan branching enzyme
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Nutrition Science (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Physiology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Diabetes (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Materials Engineering (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161556051P | 2011-11-04 | 2011-11-04 | |
| US201261645530P | 2012-05-10 | 2012-05-10 | |
| PCT/AU2012/001349 WO2013063653A1 (en) | 2011-11-04 | 2012-11-02 | High amylose wheat - ii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES3000665T3 true ES3000665T3 (en) | 2025-03-03 |
Family
ID=48191117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12846192T Active ES3000665T3 (en) | 2011-11-04 | 2012-11-02 | High amylose wheat - ii |
Country Status (6)
| Country | Link |
|---|---|
| US (4) | US9357722B2 (enExample) |
| EP (1) | EP2773183B1 (enExample) |
| JP (2) | JP6346093B2 (enExample) |
| AU (1) | AU2012327161B8 (enExample) |
| ES (1) | ES3000665T3 (enExample) |
| WO (1) | WO2013063653A1 (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE517994T1 (de) | 2003-06-30 | 2011-08-15 | Commw Scient Ind Res Org | Weizen mit veränderter verzweigungsenzymaktivität sowie daraus erhaltene stärke und stärkehaltige produkte |
| US7790955B2 (en) | 2003-10-27 | 2010-09-07 | The Commonwealth Of Australia Commonwealth Scientific And Industrial Research Organisation | Rice and products thereof having starch with an increased proportion of amylose |
| WO2008006171A1 (en) | 2006-07-14 | 2008-01-17 | Commonwealth Scientific And Industrial Research Organisation | Altering the fatty acid composition of rice |
| UA113829C2 (uk) * | 2007-11-27 | 2017-03-27 | Коммонвелс Сайнтіфік Енд Індастріал Рісерч Організейшн | Генетично модифікована рослина зі збільшеним потенціалом продуктивності |
| CA2653883C (en) | 2008-07-17 | 2022-04-05 | Colin Leslie Dow Jenkins | High fructan cereal plants |
| CN102202498B (zh) | 2008-07-21 | 2016-09-07 | 澳大利亚联邦科学与工业研究组织 | 改良的棉籽油及应用 |
| BR112012002114A2 (pt) | 2009-07-30 | 2015-09-15 | Australian Capital Ventures Ltd | cevadas e seu usos. |
| NZ610181A (en) | 2010-11-04 | 2015-03-27 | Arista Cereal Technologies Pty Ltd | High amylose wheat |
| WO2012103594A1 (en) | 2011-02-03 | 2012-08-09 | Commonwealth Scientific And Industrial Research Organisation | Barley with modified ssiii |
| AR087159A1 (es) | 2011-06-20 | 2014-02-26 | Gen Biscuit | Galletita para desayuno con glucosa de lenta disponibilidad |
| EP3539372B1 (en) | 2011-10-04 | 2023-12-06 | Arcadia Biosciences Inc. | Wheat with increased resistant starch levels |
| EP2773183B1 (en) * | 2011-11-04 | 2024-10-09 | Arista Cereal Technologies Pty Ltd | High amylose wheat - ii |
| HK1207938A1 (en) | 2012-04-25 | 2016-02-19 | Commonwealth Scientific And Industrial Research Organisation | High oleic acid oils |
| JP7102096B2 (ja) * | 2013-06-13 | 2022-07-19 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション | 非常に低レベルのホルデインを有するオオムギ |
| MY204560A (en) | 2014-07-07 | 2024-09-04 | Nuseed Global Innovation Ltd | Process for producing industrial products from plant lipids |
| WO2017132239A1 (en) * | 2016-01-26 | 2017-08-03 | Pioneer Hi-Bred International, Inc. | Waxy corn |
| JP7502028B2 (ja) * | 2016-07-05 | 2024-06-18 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション | 高アミロースコムギ－ｉｉｉ |
| WO2018039740A1 (en) | 2016-09-02 | 2018-03-08 | Commonwealth Scientific And Industrial Research Organisation | Plants with modified traits |
| AR110635A1 (es) * | 2016-09-30 | 2019-04-17 | Simplot Co J R | Cultivo de plantas de fresa en cepellón con elevación baja, sin necesidad de acondicionamiento |
| CA3099102C (en) * | 2018-05-02 | 2024-05-14 | Cellectis | Engineering wheat with increased dietary fiber |
| US11802838B2 (en) | 2020-03-23 | 2023-10-31 | Bay State Milling Company | Rapid high amylose wheat seed purity test |
| FR3120318B1 (fr) | 2021-03-02 | 2023-03-03 | Arista Cereal Tech Pty Ltd | Procédé de meunerie industrielle à rendement augmenté, farines obtenues par ce procédé, produits notamment de panification fabriqués à partir de ces farines |
| CN114145197B (zh) * | 2021-09-26 | 2023-03-28 | 中国科学院合肥物质科学研究院 | 一种提高小麦对赤霉病抗性的方法 |
| CN113881648B (zh) * | 2021-10-20 | 2022-12-13 | 江南大学 | 一种提高淀粉分支酶催化活力的方法 |
| JP7847430B2 (ja) * | 2021-12-28 | 2026-04-17 | 日清製粉株式会社 | うどん用穀粉組成物、及びこれを用いたうどんの製造方法 |
| JP7857752B2 (ja) * | 2021-12-28 | 2026-05-13 | 日清製粉株式会社 | 中華まん類用穀粉組成物、及びこれを用いた中華まん類の製造方法 |
| JP2023116159A (ja) * | 2022-02-09 | 2023-08-22 | 日清製粉株式会社 | 麺皮類用穀粉組成物、及びこれを用いた麺皮類の製造方法 |
| JP2023118310A (ja) * | 2022-02-15 | 2023-08-25 | 日清製粉株式会社 | ベーカリー用穀粉組成物、及びこれを用いたベーカリー食品の製造方法 |
| CN116064895B (zh) * | 2022-08-23 | 2025-08-12 | 河南省作物分子育种研究院 | 小麦籽粒抗性淀粉含量相关位点qRS.4DS的分子标记及其应用 |
| JP2024048150A (ja) * | 2022-09-27 | 2024-04-08 | 日清製粉株式会社 | 麺類用穀粉組成物 |
| JP2024048148A (ja) * | 2022-09-27 | 2024-04-08 | 日清製粉株式会社 | 皿うどんの製造方法 |
| JP2024048149A (ja) * | 2022-09-27 | 2024-04-08 | 日清製粉株式会社 | 茹でこぼしせずに喫食する麺類 |
| EP4770426A1 (en) * | 2023-06-02 | 2026-07-08 | Barleo ApS | Barley plant having increased amylose content |
| WO2025228942A1 (en) * | 2024-05-03 | 2025-11-06 | Société des Produits Nestlé S.A. | Edible chew having a high content of amylose for oral use by infants and young children |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3690896A (en) | 1970-05-15 | 1972-09-12 | Gen Mills Inc | Process for forming a multi-colored food product |
| US4770710A (en) | 1987-07-02 | 1988-09-13 | American Maize-Products Company | Novel starch and products produced therefrom |
| US6013861A (en) | 1989-05-26 | 2000-01-11 | Zeneca Limited | Plants and processes for obtaining them |
| US5051271A (en) | 1989-11-22 | 1991-09-24 | Opta Food Ingredients, Inc. | Starch-derived, food-grade, insoluble bulking agent |
| CA2147117C (en) | 1992-12-24 | 2002-08-20 | Kenneth J. Mcnaught | Food compositions including resistant starch |
| CN1219199A (zh) | 1995-12-20 | 1999-06-09 | 纳幕尔杜邦公司 | 通过修饰淀粉生物合成酶基因的表达而得到的新型淀粉 |
| CA2624375C (en) | 1996-05-29 | 2010-07-27 | Bayer Cropscience Gmbh | Nucleic acid molecules encoding enzymes from wheat which are involved in starch synthesis |
| WO1998037213A1 (en) | 1997-02-21 | 1998-08-27 | Danisco A/S | Antisense intron inhibition of starch branching enzyme expression |
| ATE417924T1 (de) | 1997-09-12 | 2009-01-15 | Commw Scient Ind Res Org | Regulation der genexpression in pflanzen |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| CN101818145A (zh) | 1998-03-20 | 2010-09-01 | 联邦科学和工业研究组织 | 控制基因表达 |
| DK1068311T3 (da) | 1998-04-08 | 2011-08-08 | Commw Scient Ind Res Org | Fremgangsmåder og midler til opnåelse af modificerede fænotyper |
| WO2000015810A1 (en) | 1998-09-10 | 2000-03-23 | Monsanto Plc | Isoforms of starch branching enzyme ii (sbe-iia and sbe-iib) from wheat |
| AUPQ005299A0 (en) | 1999-04-29 | 1999-05-27 | Commonwealth Scientific And Industrial Research Organisation | Novel genes encoding wheat starch synthases and uses therefor |
| CA2273673A1 (en) | 1999-06-04 | 2000-12-04 | Makoto Yamamori | High amylose wheat starch and wheat containing the same |
| US6897354B1 (en) | 1999-06-04 | 2005-05-24 | National Institute Of Agrobiological Sciences | High amylose wheat starch and wheat containing the same |
| WO2001032886A2 (en) | 1999-10-29 | 2001-05-10 | National Research Council Of Canada | Starch branching enzymes |
| US7041484B1 (en) | 1999-10-29 | 2006-05-09 | National Research Council Of Canada | Starch branching enzymes |
| US6326193B1 (en) | 1999-11-05 | 2001-12-04 | Cambria Biosciences, Llc | Insect control agent |
| AUPQ574200A0 (en) | 2000-02-21 | 2000-03-16 | Commonwealth Scientific And Industrial Research Organisation | Starch branching enzyme |
| US20020002713A1 (en) | 2000-03-01 | 2002-01-03 | Allen Stephen M. | Starch branching enzyme IIb |
| GB2360521A (en) | 2000-03-20 | 2001-09-26 | Danisco | Genetic modification of starch in plants |
| PT1331845E (pt) | 2000-11-09 | 2012-04-12 | Commw Scient Ind Res Org | Cevada com reduzida atividade ssii e produtos contendo amido com um conteúdo de amilopectina reduzido |
| US20030035857A1 (en) | 2001-03-22 | 2003-02-20 | Sroka Pongpun Elisa | Process for preparing wheat bread and dough mix for same |
| US7009092B1 (en) | 2001-06-04 | 2006-03-07 | Iowa State University Research Foundation | Transgenic corn plants having seeds with modified cornstarch characteristics and method of making the transgenic corn plants |
| ES2305257T3 (es) | 2001-06-12 | 2008-11-01 | Bayer Cropscience Ag | Plantas transgenicas que sintetizan almidon rico en amilosa. |
| JP4308002B2 (ja) | 2001-09-10 | 2009-08-05 | 独立行政法人科学技術振興機構 | デンプン合成酵素類 |
| AUPS219802A0 (en) | 2002-05-09 | 2002-06-06 | Commonwealth Scientific And Industrial Research Organisation | Barley with altered branching enzyme activity and starch and starch containing products with a reduced amylopectin content |
| CA2516310C (en) | 2003-02-19 | 2014-12-09 | Commonwealth Scientific And Industrial Research Organisation | Efficient gene silencing in plants using short dsrna sequences |
| ATE517994T1 (de) * | 2003-06-30 | 2011-08-15 | Commw Scient Ind Res Org | Weizen mit veränderter verzweigungsenzymaktivität sowie daraus erhaltene stärke und stärkehaltige produkte |
| US7790955B2 (en) | 2003-10-27 | 2010-09-07 | The Commonwealth Of Australia Commonwealth Scientific And Industrial Research Organisation | Rice and products thereof having starch with an increased proportion of amylose |
| CA2594155C (en) * | 2004-12-30 | 2016-08-30 | Commonwealth Scientific And Industrial Research Organisation | A process for producing a food or beverage |
| US7993686B2 (en) | 2004-12-30 | 2011-08-09 | Commonwealth Scientific And Industrial Organisation | Method and means for improving bowel health |
| US7700139B2 (en) | 2004-12-30 | 2010-04-20 | Commonwealth Scientific And Industrial Research Organization | Method and means for improving bowel health |
| UA113829C2 (uk) | 2007-11-27 | 2017-03-27 | Коммонвелс Сайнтіфік Енд Індастріал Рісерч Організейшн | Генетично модифікована рослина зі збільшеним потенціалом продуктивності |
| NZ610181A (en) * | 2010-11-04 | 2015-03-27 | Arista Cereal Technologies Pty Ltd | High amylose wheat |
| EP3539372B1 (en) | 2011-10-04 | 2023-12-06 | Arcadia Biosciences Inc. | Wheat with increased resistant starch levels |
| EP2773183B1 (en) | 2011-11-04 | 2024-10-09 | Arista Cereal Technologies Pty Ltd | High amylose wheat - ii |
-
2012
- 2012-11-02 EP EP12846192.8A patent/EP2773183B1/en active Active
- 2012-11-02 ES ES12846192T patent/ES3000665T3/es active Active
- 2012-11-02 WO PCT/AU2012/001349 patent/WO2013063653A1/en not_active Ceased
- 2012-11-02 US US13/668,177 patent/US9357722B2/en active Active
- 2012-11-02 JP JP2014539188A patent/JP6346093B2/ja active Active
- 2012-11-02 AU AU2012327161A patent/AU2012327161B8/en active Active
-
2016
- 2016-05-16 US US15/156,066 patent/US10798957B2/en active Active
-
2018
- 2018-01-05 JP JP2018000651A patent/JP7182878B2/ja active Active
-
2020
- 2020-09-14 US US17/019,960 patent/US11737482B2/en active Active
-
2023
- 2023-08-28 US US18/456,986 patent/US20240122218A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2773183A1 (en) | 2014-09-10 |
| EP2773183B1 (en) | 2024-10-09 |
| US11737482B2 (en) | 2023-08-29 |
| US9357722B2 (en) | 2016-06-07 |
| JP6346093B2 (ja) | 2018-06-20 |
| AU2012327161A8 (en) | 2016-12-08 |
| US20170064986A1 (en) | 2017-03-09 |
| AU2012327161A1 (en) | 2013-06-20 |
| US20130115362A1 (en) | 2013-05-09 |
| US20210161185A1 (en) | 2021-06-03 |
| WO2013063653A1 (en) | 2013-05-10 |
| JP7182878B2 (ja) | 2022-12-05 |
| US10798957B2 (en) | 2020-10-13 |
| JP2015504301A (ja) | 2015-02-12 |
| AU2012327161B2 (en) | 2016-11-03 |
| AU2012327161B8 (en) | 2016-12-08 |
| US20240122218A1 (en) | 2024-04-18 |
| JP2018075031A (ja) | 2018-05-17 |
| EP2773183A4 (en) | 2015-04-15 |
| EP2773183C0 (en) | 2024-10-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12075806B2 (en) | Food ingredients produced from high amylose wheat | |
| US20240122218A1 (en) | High amylose wheat- ii | |
| KR101806299B1 (ko) | 보리 및 이의 용도 | |
| JP4923171B2 (ja) | 分枝酵素の活性を改変した小麦、ならびにそれから得たデンプンおよびデンプン含有品 | |
| US20190338299A1 (en) | High amylose wheat - iii | |
| ES2369774T3 (es) | Trigo con actividad de enzima ramificante y almidón alterados y productos que contienen almidón derivados del mismo. |