ES2922478T3 - Método para detectar y cuantificar una molécula deseada en un tejido - Google Patents
Método para detectar y cuantificar una molécula deseada en un tejido Download PDFInfo
- Publication number
- ES2922478T3 ES2922478T3 ES12731087T ES12731087T ES2922478T3 ES 2922478 T3 ES2922478 T3 ES 2922478T3 ES 12731087 T ES12731087 T ES 12731087T ES 12731087 T ES12731087 T ES 12731087T ES 2922478 T3 ES2922478 T3 ES 2922478T3
- Authority
- ES
- Spain
- Prior art keywords
- tissue
- molecule
- calibration
- homogenized
- interest
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 79
- 238000010790 dilution Methods 0.000 claims abstract description 59
- 239000012895 dilution Substances 0.000 claims abstract description 59
- 238000004458 analytical method Methods 0.000 claims abstract description 52
- 230000001143 conditioned effect Effects 0.000 claims abstract description 30
- 238000011002 quantification Methods 0.000 claims description 51
- 239000011159 matrix material Substances 0.000 claims description 47
- 238000004949 mass spectrometry Methods 0.000 claims description 31
- 238000001514 detection method Methods 0.000 claims description 26
- 238000002360 preparation method Methods 0.000 claims description 23
- 230000003750 conditioning effect Effects 0.000 claims description 20
- 241001465754 Metazoa Species 0.000 claims description 17
- 238000000227 grinding Methods 0.000 claims description 16
- 238000003384 imaging method Methods 0.000 claims description 14
- 238000001819 mass spectrum Methods 0.000 claims description 14
- 238000003908 quality control method Methods 0.000 claims description 13
- 238000009826 distribution Methods 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 238000000376 autoradiography Methods 0.000 claims description 5
- 230000008033 biological extinction Effects 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 150000002632 lipids Chemical class 0.000 claims description 5
- 238000012921 fluorescence analysis Methods 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 239000002207 metabolite Substances 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 268
- 239000000523 sample Substances 0.000 description 77
- AQHHHDLHHXJYJD-UHFFFAOYSA-N propranolol Chemical compound C1=CC=C2C(OCC(O)CNC(C)C)=CC=CC2=C1 AQHHHDLHHXJYJD-UHFFFAOYSA-N 0.000 description 72
- 210000003734 kidney Anatomy 0.000 description 43
- 229960003712 propranolol Drugs 0.000 description 36
- 230000000875 corresponding effect Effects 0.000 description 18
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 17
- 150000001875 compounds Chemical class 0.000 description 15
- 239000000463 material Substances 0.000 description 13
- 210000000056 organ Anatomy 0.000 description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- 230000003595 spectral effect Effects 0.000 description 9
- 238000000151 deposition Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 239000011248 coating agent Substances 0.000 description 6
- 238000000576 coating method Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 238000011088 calibration curve Methods 0.000 description 4
- 230000008021 deposition Effects 0.000 description 4
- 238000004590 computer program Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000003795 desorption Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000265 homogenisation Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 238000005192 partition Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000010200 validation analysis Methods 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000004252 FT/ICR mass spectrometry Methods 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 238000002425 crystallisation Methods 0.000 description 2
- 230000008025 crystallization Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 238000010191 image analysis Methods 0.000 description 2
- 238000000091 laser ablation electrospray ionisation Methods 0.000 description 2
- 238000001698 laser desorption ionisation Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000010606 normalization Methods 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- OKTJSMMVPCPJKN-NJFSPNSNSA-N Carbon-14 Chemical compound [14C] OKTJSMMVPCPJKN-NJFSPNSNSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 208000027089 Parkinsonian disease Diseases 0.000 description 1
- 206010034010 Parkinsonism Diseases 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000000688 desorption electrospray ionisation Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000001871 ion mobility spectroscopy Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 238000000095 laser ablation inductively coupled plasma mass spectrometry Methods 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- DIVDFFZHCJEHGG-UHFFFAOYSA-N oxidopamine Chemical compound NCCC1=CC(O)=C(O)C=C1O DIVDFFZHCJEHGG-UHFFFAOYSA-N 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011158 quantitative evaluation Methods 0.000 description 1
- 210000005084 renal tissue Anatomy 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000005211 surface analysis Methods 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N2001/2893—Preparing calibration standards
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Optics & Photonics (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1154731A FR2976076B1 (fr) | 2011-05-31 | 2011-05-31 | Procede de detection et de quantification d'une molecule cible dans un tissu |
| PCT/FR2012/051205 WO2012164221A1 (fr) | 2011-05-31 | 2012-05-29 | Procede de detection et de quantification d'une molecule cible dans un tissu |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2922478T3 true ES2922478T3 (es) | 2022-09-15 |
Family
ID=46420402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12731087T Active ES2922478T3 (es) | 2011-05-31 | 2012-05-29 | Método para detectar y cuantificar una molécula deseada en un tejido |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10132796B2 (https=) |
| EP (1) | EP2715310B1 (https=) |
| JP (1) | JP6170911B2 (https=) |
| KR (1) | KR102004350B1 (https=) |
| CN (1) | CN103688151B (https=) |
| AU (1) | AU2012264458B2 (https=) |
| CA (1) | CA2837153C (https=) |
| DK (1) | DK2715310T3 (https=) |
| ES (1) | ES2922478T3 (https=) |
| FR (1) | FR2976076B1 (https=) |
| IN (1) | IN2013MN02451A (https=) |
| WO (1) | WO2012164221A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2693037T3 (es) * | 2013-02-25 | 2018-12-07 | Imabiotech | Método para evaluar la acción selectiva de una molécula de interés por un tejido |
| CN103336128A (zh) * | 2013-06-08 | 2013-10-02 | 天津药物研究院 | 一种检测小分子多肽类药物组织分布的方法 |
| EP3371594A1 (en) * | 2015-11-06 | 2018-09-12 | Ventana Medical Systems, Inc. | Representative diagnostics |
| KR101996666B1 (ko) * | 2016-05-02 | 2019-07-04 | 주식회사 엘지화학 | 정량 분석 방법 |
| JP7010373B2 (ja) | 2018-05-30 | 2022-01-26 | 株式会社島津製作所 | スペクトルデータ処理装置及び分析装置 |
| CN115836210A (zh) * | 2020-02-27 | 2023-03-21 | Lsk 技术公司 | 使用光学反应表征来自感兴趣区域的化验的系统和方法 |
| WO2021175842A1 (en) | 2020-03-02 | 2021-09-10 | Universiteit Maastricht | Quality control standards for mass spectrometry imaging |
| CN115326915A (zh) * | 2022-08-01 | 2022-11-11 | 中央民族大学 | 一种植物组织微阵列maldi-msi高通量分析方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60025526T2 (de) * | 1999-11-16 | 2006-08-24 | Genentech, Inc., South San Francisco | Elisa für vegf |
| AU2003272924B2 (en) * | 2002-10-04 | 2008-05-15 | Protosera Inc. | Plate for mass spectrometry, process for preparing the same and use thereof |
| US20040096907A1 (en) * | 2002-11-06 | 2004-05-20 | Bernd Bohrmann | Quantification of beta amyloid |
| JP2005181011A (ja) * | 2003-12-17 | 2005-07-07 | Yoshio Yamauchi | タンパク質解析方法 |
| JP4547173B2 (ja) | 2004-03-17 | 2010-09-22 | シスメックス株式会社 | 糖尿病診療支援システム |
| WO2007000669A2 (en) | 2005-06-07 | 2007-01-04 | Centre National De La Recherche Scientifique (Cnrs) | Use of conjugates with linkers cleavable by photodissociation or fragmentation for mass spectrometry analysis of tissue sections |
| EP2163900A1 (en) | 2008-09-04 | 2010-03-17 | Commissariat A L'energie Atomique | New method of imaging by mass spectrometry and new mass tag associated trityl derivatives |
| WO2010041459A1 (ja) * | 2008-10-08 | 2010-04-15 | 積水メディカル株式会社 | 薬物代謝物の定量分析方法及び分析装置 |
| WO2011073740A1 (en) | 2009-12-15 | 2011-06-23 | Centre National De La Recherche Scientifique (Cnrs) | Matrices for mass spectrometry imaging |
| FR2973112B1 (fr) | 2011-03-21 | 2018-05-25 | Imabiotech | Procede de detection et de quantification d'une molecule cible dans un echantillon |
| EP2767832A1 (en) | 2013-02-18 | 2014-08-20 | Imabiotech | Photo or chemolabile conjugates for molecules detection |
| ES2693037T3 (es) | 2013-02-25 | 2018-12-07 | Imabiotech | Método para evaluar la acción selectiva de una molécula de interés por un tejido |
-
2011
- 2011-05-31 FR FR1154731A patent/FR2976076B1/fr active Active
-
2012
- 2012-05-29 IN IN2451MUN2013 patent/IN2013MN02451A/en unknown
- 2012-05-29 JP JP2014513236A patent/JP6170911B2/ja active Active
- 2012-05-29 US US14/123,128 patent/US10132796B2/en active Active
- 2012-05-29 KR KR1020137035169A patent/KR102004350B1/ko active Active
- 2012-05-29 CN CN201280026907.4A patent/CN103688151B/zh active Active
- 2012-05-29 CA CA2837153A patent/CA2837153C/fr active Active
- 2012-05-29 EP EP12731087.8A patent/EP2715310B1/fr active Active
- 2012-05-29 WO PCT/FR2012/051205 patent/WO2012164221A1/fr not_active Ceased
- 2012-05-29 DK DK12731087.8T patent/DK2715310T3/da active
- 2012-05-29 AU AU2012264458A patent/AU2012264458B2/en active Active
- 2012-05-29 ES ES12731087T patent/ES2922478T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2837153A1 (fr) | 2012-12-06 |
| CN103688151A (zh) | 2014-03-26 |
| WO2012164221A1 (fr) | 2012-12-06 |
| FR2976076A1 (fr) | 2012-12-07 |
| JP2014520259A (ja) | 2014-08-21 |
| EP2715310A1 (fr) | 2014-04-09 |
| US10132796B2 (en) | 2018-11-20 |
| AU2012264458A1 (en) | 2014-01-09 |
| CN103688151B (zh) | 2016-05-18 |
| AU2012264458B2 (en) | 2015-09-24 |
| CA2837153C (fr) | 2019-06-25 |
| KR102004350B1 (ko) | 2019-07-26 |
| IN2013MN02451A (https=) | 2015-04-17 |
| JP6170911B2 (ja) | 2017-07-26 |
| KR20140051187A (ko) | 2014-04-30 |
| DK2715310T3 (da) | 2022-07-25 |
| EP2715310B1 (fr) | 2022-04-27 |
| US20140106391A1 (en) | 2014-04-17 |
| FR2976076B1 (fr) | 2015-02-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2922478T3 (es) | Método para detectar y cuantificar una molécula deseada en un tejido | |
| Neumann et al. | Spatial metabolomics of the human kidney using MALDI trapped ion mobility imaging mass spectrometry | |
| Hermann et al. | Sample preparation of formalin-fixed paraffin-embedded tissue sections for MALDI-mass spectrometry imaging | |
| Norris et al. | Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research | |
| Cornett et al. | MALDI imaging mass spectrometry: molecular snapshots of biochemical systems | |
| Seeley et al. | Molecular imaging of proteins in tissues by mass spectrometry | |
| ES2745648T3 (es) | Método para detectar y cuantificar una molécula diana en una muestra | |
| Fung et al. | Emerging role of clinical mass spectrometry in pathology | |
| EP2124192B1 (en) | Method for the analysis of tissue sections | |
| Chaurand | Imaging mass spectrometry of thin tissue sections: a decade of collective efforts | |
| Bonta et al. | A comparison of sample preparation strategies for biological tissues and subsequent trace element analysis using LA-ICP-MS | |
| Ryan et al. | Protein identification in imaging mass spectrometry through spatially targeted liquid micro‐extractions | |
| Wisztorski et al. | MALDI direct analysis and imaging of frozen versus FFPE tissues: what strategy for which sample? | |
| Byliński et al. | Direct analysis of samples of various origin and composition using specific types of mass spectrometry | |
| CN108693002B (zh) | 一种模拟生物组织薄片、其制备方法及其应用与装置 | |
| Zubair et al. | Trypsin and MALDI matrix pre‐coated targets simplify sample preparation for mapping proteomic distributions within biological tissues by imaging mass spectrometry | |
| Veerasammy et al. | Sample preparation for metabolic profiling using MALDI mass spectrometry imaging | |
| Végvári et al. | Essential tactics of tissue preparation and matrix nano-spotting for successful compound imaging mass spectrometry | |
| Quanico et al. | Combined MALDI mass spectrometry imaging and parafilm-assisted microdissection-based LC-MS/MS workflows in the study of the brain | |
| Lukowski et al. | An optimized approach and inflation media for obtaining complimentary mass spectrometry-based omics data from human lung tissue | |
| Zhao et al. | Mass spectrometry imaging: applications in drug distribution studies | |
| WO2023076662A1 (en) | System and method for quantitative pathology using mass spectrometry imaging | |
| Chicano-Galvez et al. | MALDI mass spectrometry imaging and spatially-resolved proteomics | |
| US20260126452A1 (en) | System and method for quantitative pathology using mass spectrometry imaging | |
| Deng et al. | Deepening imaging-based spatial proteomics at high spatial resolution through controlled tissue resizing and in-situ bottom-up mass spectrometry |