ES2898272T3 - Microorganismos y métodos para producir cannabinoides y derivados de cannabinoides - Google Patents

Microorganismos y métodos para producir cannabinoides y derivados de cannabinoides Download PDF

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ES2898272T3
ES2898272T3 ES18728259T ES18728259T ES2898272T3 ES 2898272 T3 ES2898272 T3 ES 2898272T3 ES 18728259 T ES18728259 T ES 18728259T ES 18728259 T ES18728259 T ES 18728259T ES 2898272 T3 ES2898272 T3 ES 2898272T3
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seq
polypeptide
amino acid
nucleic acids
heterologous nucleic
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Spanish (es)
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Jay D Keasling
Leo D'espaux
Jeff Wong
Xiaozhou Luo
Michael Reiter
Charles Denby
Anna Lechner
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University of California Berkeley
University of California San Diego UCSD
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University of California San Diego UCSD
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C63/00Compounds having carboxyl groups bound to a carbon atoms of six-membered aromatic rings
    • C07C63/04Monocyclic monocarboxylic acids
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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    • C12P7/00Preparation of oxygen-containing organic compounds
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    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01102Geranyl-pyrophosphate—olivetolic acid geranyltransferase (2.5.1.102)
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
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    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01088Hydroxymethylglutaryl-CoA reductase (1.1.1.88)
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    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01086Fatty-acyl-CoA synthase (2.3.1.86)
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    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/012063,5,7-Trioxododecanoyl-CoA synthase (2.3.1.206)
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    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/03Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/0301Hydroxymethylglutaryl-CoA synthase (2.3.3.10)
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    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01036Mevalonate kinase (2.7.1.36)
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    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04002Phosphomevalonate kinase (2.7.4.2)
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    • C12Y401/00Carbon-carbon lyases (4.1)
    • C12Y401/01Carboxy-lyases (4.1.1)
    • C12Y401/01033Diphosphomevalonate decarboxylase (4.1.1.33), i.e. mevalonate-pyrophosphate decarboxylase
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    • C12Y503/00Intramolecular oxidoreductases (5.3)
    • C12Y503/03Intramolecular oxidoreductases (5.3) transposing C=C bonds (5.3.3)
    • C12Y503/03002Isopentenyl-diphosphate DELTA-isomerase (5.3.3.2)
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    • C12Y602/00Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01Acid-Thiol Ligases (6.2.1)
    • C12Y602/01003Long-chain-fatty-acid-CoA ligase (6.2.1.3)
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    • C12Y604/00Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002Acetyl-CoA carboxylase (6.4.1.2)

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  • Chemical & Material Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Biophysics (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
ES18728259T 2017-04-27 2018-04-27 Microorganismos y métodos para producir cannabinoides y derivados de cannabinoides Active ES2898272T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762491114P 2017-04-27 2017-04-27
US201762569532P 2017-10-07 2017-10-07
PCT/US2018/029668 WO2018200888A1 (en) 2017-04-27 2018-04-27 Microorganisms and methods for producing cannabinoids and cannabinoid derivatives

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ES2898272T3 true ES2898272T3 (es) 2022-03-04

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US (4) US10563211B2 (enExample)
EP (2) EP3615667B1 (enExample)
JP (1) JP7198555B2 (enExample)
CN (1) CN110914416B (enExample)
AU (1) AU2018256863B2 (enExample)
BR (1) BR112019022500A2 (enExample)
CA (1) CA3061718A1 (enExample)
ES (1) ES2898272T3 (enExample)
IL (1) IL270202B2 (enExample)
SG (1) SG11201910019PA (enExample)
WO (1) WO2018200888A1 (enExample)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11180781B2 (en) * 2016-08-21 2021-11-23 Insectergy, Llc Biosynthetic cannabinoid production methods
US10239808B1 (en) 2016-12-07 2019-03-26 Canopy Holdings, LLC Cannabis extracts
AU2018220470B2 (en) 2017-02-17 2022-02-24 Hyasynth Biologicals Inc. Method and cell line for production of polyketides in yeast
EP3615667B1 (en) * 2017-04-27 2021-08-11 The Regents of The University of California Microorganisms and methods for producing cannabinoids and cannabinoid derivatives
WO2018209143A1 (en) 2017-05-10 2018-11-15 Baymedica, Inc. Recombinant production systems for prenylated polyketides of the cannabinoid family
EP3652327A4 (en) 2017-07-12 2021-04-21 Biomedican, Inc. Production of cannabinoids in yeast
EP3692143A4 (en) * 2017-10-05 2021-09-29 Eleszto Genetika, Inc. MICRO-ORGANISMS AND PROCESSES FOR THE FERMENTATION OF CANNABINOIDS
EP3745884A1 (en) 2018-01-31 2020-12-09 Canopy Holdings, Llc Hemp powder
US11746351B2 (en) 2018-04-30 2023-09-05 Algae-C Inc. Engineered microorganism for the production of cannabinoid biosynthetic pathway products
US10801049B2 (en) 2018-06-14 2020-10-13 Syntiva Therapeutics, Inc. Genetically engineered microorganisms and processes for the production of cannabinoids from a carbon source precursor
CA3107544A1 (en) 2018-08-01 2020-02-06 The Regents Of The University Of California Biosynthetic platform for the production of cannabinoids and other prenylated compounds
WO2020060948A1 (en) 2018-09-17 2020-03-26 Levadura Biotechnology, Inc. Production of cannabinoids in yeast using a fatty acid feedstock
WO2020069214A2 (en) * 2018-09-26 2020-04-02 Demetrix, Inc. Optimized expression systems for producing cannabinoid synthase polypeptides, cannabinoids, and cannabinoid derivatives
US11040932B2 (en) 2018-10-10 2021-06-22 Treehouse Biotech, Inc. Synthesis of cannabigerol
US20220002764A1 (en) * 2018-11-14 2022-01-06 Manus Bio Inc. Microbial cells and methods for producing cannabinoids
CN113366009B (zh) 2018-11-27 2025-02-11 科纳科学控股有限公司 用于生物合成大麻素的双向多酶支架
WO2020160284A1 (en) 2019-01-30 2020-08-06 Genomatica, Inc. Recovery, decarboxylation, and purification of cannabinoids from engineered cell cultures
US20220127649A1 (en) * 2019-01-30 2022-04-28 Genomatica, Inc. Engineered cells for improved production of cannabinoids
WO2020161682A1 (en) * 2019-02-10 2020-08-13 Dyadic International (Usa), Inc. Production of cannabinoids in filamentous fungi
WO2020169221A1 (en) * 2019-02-20 2020-08-27 Synbionik Gmbh Production of plant-based active substances (e.g. cannabinoids) by recombinant microorganisms
EP3931330A4 (en) 2019-02-25 2023-03-15 Ginkgo Bioworks, Inc. BIOSYNTHESIS OF CANNABINOIDS AND CANNABINOID PRECURSORS
US20220243236A1 (en) * 2019-03-01 2022-08-04 The Regents Of The University Of California Production of cannabinoids using genetically engineered photosynthetic microorganisms
US20220127620A1 (en) * 2019-03-15 2022-04-28 Andrew P. Klein Microbial production of compounds
WO2020198679A1 (en) * 2019-03-27 2020-10-01 Rynetech Bio, Inc. Biosynthetic cannabinoid production in engineered microorganisms
WO2020208411A2 (en) * 2019-04-11 2020-10-15 Eleszto Genetika, Inc. Microorganisms and methods for the fermentation of cannabinoids
WO2020210810A1 (en) * 2019-04-12 2020-10-15 Renew Biopharma, Inc. Compositions and methods for using genetically modified enzymes
SG11202111975UA (en) * 2019-05-22 2021-11-29 Demetrix Inc Optimized cannabinoid synthase polypeptides
WO2020247741A1 (en) * 2019-06-06 2020-12-10 Genomatica, Inc. Olivetolic acid cyclase variants and methods for their use
US12036485B1 (en) * 2019-07-16 2024-07-16 Green Vault Systems, LLC Continuous flow cold water extraction
CA3148950A1 (en) * 2019-07-30 2021-02-04 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (A.R.O.), Volcani Center Methods of controlling cannabinoid synthesis in plants or cells and plants and cells produced thereby
CA3151799A1 (en) * 2019-08-18 2021-02-25 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
WO2021041572A1 (en) * 2019-08-27 2021-03-04 Natural Extraction Systems, LLC Compositions comprising decarboxylated cannabinoids
CA3149245A1 (en) * 2019-08-30 2021-03-04 David F. PREINER Production of gpp and cbga in a methylotrophic yeast strain
WO2021042057A1 (en) * 2019-08-30 2021-03-04 Lygos, Inc. Systems and methods for preparing cannabinoids and derivatives
BR112022004797A2 (pt) * 2019-09-18 2022-06-21 Demetrix Inc Polipeptídeos otimizados de ácido tetra-hidrocanabinólico (thca) sintase
CN114729386B (zh) * 2019-10-01 2025-06-13 杭州恩和生物科技有限公司 用于大麻素合成的酶及其制备和使用方法
WO2021071438A1 (en) * 2019-10-11 2021-04-15 National University Of Singapore Biosynthesis of cannabinoids from cannabigerolic acid using novel cannabinoid synthases
AU2020364933A1 (en) * 2019-10-11 2022-04-21 National University Of Singapore Sustainable production of cannabinoids from simple precursor feedstocks using
CN114555797B (zh) * 2019-10-11 2024-10-01 新加坡国立大学 使用芳香异戊二烯基转移酶生物合成大麻素前体
CN110669713A (zh) * 2019-10-18 2020-01-10 中国科学院青岛生物能源与过程研究所 一种合成d-柠檬烯的基因工程菌及其构建方法与应用
IL292380A (en) * 2019-10-29 2022-06-01 Algae C Inc A genetically engineered bacterium to make cannabinoids
WO2021081648A1 (en) * 2019-10-29 2021-05-06 Algae-C Inc. Engineered microorganism for the production of cannabinoid biosynthetic pathway products
BR112022013503A2 (pt) * 2020-01-10 2022-09-13 Univ California Plataforma biossintética para a produção de ácido olivetólico e análogos de ácido olivetólico
EP4087933A1 (en) * 2020-01-10 2022-11-16 Barrit Sarl Production of bioactive bibenzylic acid or derivatives thereof by genetically modified microbial hosts
US20230063396A1 (en) * 2020-01-20 2023-03-02 Baymedica, Inc. Genetically modified yeast for the production of cannabigerolic acid, cannabichromenic acid and related cannabinoids
CN113999870B (zh) * 2020-02-26 2024-02-20 森瑞斯生物科技(深圳)有限公司 一种表达cbdas的重组酿酒酵母及其构建方法和应用
US20230087321A1 (en) * 2020-03-03 2023-03-23 BetterSeeds Ltd. Odorless cannabis plant
WO2021183448A1 (en) 2020-03-09 2021-09-16 Demetrix, Inc. Optimized olivetolic acid cyclase polypeptides
CA3172499A1 (en) 2020-03-27 2021-09-30 Mathias Schuetz Compositions and methods for recombinant biosynthesis of cannabinoids
US12241104B2 (en) 2020-04-01 2025-03-04 The Regents Of The University Of California Use of metal salts and deep eutectic solvents in a process to solubilize a biomass
US12392085B2 (en) 2020-04-28 2025-08-19 The Regents Of The University Of California Use of in-situ ionic liquid (IL) and deep eutectic solvent (DES) synthesis using chemically synthesized or biomass-derived ions in the pretreatment of biomass
US12371855B2 (en) 2020-04-28 2025-07-29 The Regents Of The University Of California Use of ensiled biomass for increased efficiency of the pretreatment of biomass
EP4142711A1 (en) 2020-04-29 2023-03-08 Willow Biosciences Inc. Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
CA3177968A1 (en) * 2020-05-08 2021-11-11 Nick OHLER Large scale production of olivetol, olivetolic acid and other alkyl resorcinols by fermentation
CN113930400B (zh) * 2020-06-29 2025-06-13 中国科学院分子植物科学卓越创新中心 一叶萩来源的氧化酶及其应用
CA3186712A1 (en) * 2020-07-24 2022-01-27 Alexander James CAMPBELL Methods and cells with modifying enzymes for producing substituted cannabinoids and precursors
EP4200426A4 (en) * 2020-08-19 2024-10-16 Amyris, Inc. Microbial production of cannabinoids
CN112063647B (zh) * 2020-09-17 2023-05-02 云南农业大学 酿酒酵母重组菌Cuol01的构建方法、酿酒酵母重组菌Cuol02及应用
CN112280699B (zh) * 2020-09-28 2022-06-21 嘉兴欣贝莱生物科技有限公司 生产戊基二羟基苯酸的方法
CA3177870A1 (en) * 2020-10-13 2022-04-21 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
CN112410235A (zh) * 2020-11-23 2021-02-26 森瑞斯生物科技(深圳)有限公司 一种高产大麻萜酚的酿酒酵母菌株及其构建方法和应用
US11884620B2 (en) 2020-12-11 2024-01-30 The Regents Of The University Of California Use of polyamines in the pretreatment of biomass
EP4259772A1 (en) 2020-12-11 2023-10-18 Willow Biosciences, Inc. Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors
US12098171B2 (en) 2020-12-11 2024-09-24 The Regents Of The University Of California Hybrid sugar transporters with altered sugar transport activity and uses thereof
CN112795495B (zh) * 2020-12-14 2021-10-26 大连理工大学 利用酿酒酵母生产异源大麻环萜酚的方法
CN113234845B (zh) * 2021-03-12 2023-03-24 内蒙古农业大学 鉴别内蒙古地区主栽枣品种的snp分子标记引物及标记方法
WO2022204007A2 (en) * 2021-03-22 2022-09-29 Willow Biosciences, Inc. Recombinant polypeptides for enhanced biosynthesis of cannabinoids
US20240228986A1 (en) * 2021-05-14 2024-07-11 Cellibre, Inc. Engineered cells, enzymes, and methods for producing cannabinoids
CN113278597B (zh) * 2021-05-26 2023-04-21 重庆大学 新型短侧链脂肪酸CoA连接酶及其在制备广藿香酮中的应用
WO2022256697A1 (en) * 2021-06-04 2022-12-08 Amyris, Inc. Methods of purifying cannabinoids
CN113528365B (zh) * 2021-07-13 2023-04-07 浙江寿仙谷医药股份有限公司 产大麻二酚的重组酿酒酵母、其构建方法以及应用
EP4377451A2 (en) 2021-07-30 2024-06-05 Willow Biosciences, Inc. Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids
WO2023023621A1 (en) 2021-08-19 2023-02-23 Willow Biosciences, Inc. Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN114196645B (zh) * 2021-09-10 2022-08-09 北京蓝晶微生物科技有限公司 一种橄榄醇合成酶变体l及其用途
CN113502255B (zh) * 2021-09-10 2022-01-28 北京蓝晶微生物科技有限公司 用于生产橄榄醇和橄榄醇酸的工程化微生物
WO2023069921A1 (en) 2021-10-19 2023-04-27 Epimeron Usa, Inc. Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN114214339A (zh) * 2021-12-08 2022-03-22 福建农林大学 一种汉麻thcsas2基因及其编码产物萜烯酚酸氧化环化酶与应用
CN114591923B (zh) * 2022-05-10 2022-08-30 森瑞斯生物科技(深圳)有限公司 大麻二酚酸合成酶突变体及其构建方法与应用
US20250354180A1 (en) * 2022-05-12 2025-11-20 Manus Bio Inc. Enzymes, cells, and methods for producing cis-3 hexenol
WO2024042486A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the production of polyurethanes
WO2024042405A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the synthesis of bio-based polymers
EP4627098A1 (en) 2022-12-02 2025-10-08 Amyris Bio Products Portugal, Unipessoal, LDA Compositions and methods for using previously cultured cells
CN116024111B (zh) * 2022-12-03 2025-09-05 中国科学院深圳先进技术研究院 一种产大麻萜酚的微生物细胞及其构建方法与应用
CN116622784B (zh) * 2023-02-14 2024-03-01 黑龙江八一农垦大学 一种大麻二酚酸合成酶的应用
CN116574700B (zh) * 2023-05-12 2023-11-14 黑龙江八一农垦大学 大麻二酚酸合成酶突变体及其应用
WO2024254488A1 (en) 2023-06-09 2024-12-12 Amyris, Inc. Improved overlays for cannabinoid production

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5545818A (en) 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
US5545817A (en) 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
KR100445103B1 (ko) 1994-12-09 2004-12-04 임페리얼 컬리지 이노베이션스 리미티드 유전자의동정
US6900012B1 (en) 1997-06-03 2005-05-31 The University Of Chicago Plant artificial chromosome compositions and methods
US6080849A (en) 1997-09-10 2000-06-27 Vion Pharmaceuticals, Inc. Genetically modified tumor-targeted bacteria with reduced virulence
US20030033626A1 (en) 2000-07-31 2003-02-13 Hahn Frederick M. Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
GB0105924D0 (en) 2001-03-09 2001-04-25 Microscience Ltd Promoter
US6733996B2 (en) 2002-08-26 2004-05-11 Trustees Of Dartmouth College Methods for regulating gene expression using light
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
CA2651747C (en) 2006-05-26 2017-10-24 Amyris Biotechnologies, Inc. Production of isoprenoids
BRPI0823506A8 (pt) 2007-12-13 2018-05-15 Danisco Us Inc Genencor Div composições e métodos para a produção de isoprene
JP2011518564A (ja) 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体
EP2310490B1 (en) 2008-07-02 2019-01-02 Danisco US Inc. Methods for producing isoprene
US8884100B2 (en) 2009-08-12 2014-11-11 National Research Council Of Canada Aromatic prenyltransferase from Cannabis
WO2011127589A1 (en) 2010-04-15 2011-10-20 National Research Council Of Canada Genes and proteins for aromatic polyketide synthesis
DK2732037T3 (en) 2011-07-13 2018-02-26 Nat Res Council Canada GENES AND PROTEINS FOR ALKANOYL-COA SYNTHESIS
JP6581509B2 (ja) 2013-02-28 2019-09-25 ティーウィノット テクノロジーズ リミテッド 化合物を合成する化学工学プロセス及び装置
WO2014159688A1 (en) 2013-03-14 2014-10-02 Sc Laboratories, Inc. Bioactive concentrates and uses thereof
NZ711538A (en) 2013-03-15 2017-03-31 Univ Leland Stanford Junior Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same
WO2015196275A1 (en) 2014-06-27 2015-12-30 National Research Council Of Canada (Nrc) Cannabichromenic acid synthase from cannabis sativa
US9822384B2 (en) 2014-07-14 2017-11-21 Librede Inc. Production of cannabinoids in yeast
AU2015308136B2 (en) 2014-08-25 2020-07-09 Teewinot Technologies Limited Apparatus and methods for the simultaneous production of cannabinoid compounds
WO2016123475A1 (en) 2015-01-31 2016-08-04 Constance Therapeutics, Inc. Methods for preparation of cannabis oil extracts and compositions
EP3067058A1 (en) * 2015-03-13 2016-09-14 Farmagens Health Care Srl Biological composition based on engineered lactobacillus paracasei subsp. paracasei f19 for the biosynthesis of cannabinoids
US20160298151A1 (en) 2015-04-09 2016-10-13 Sher Ali Butt Novel Method for the cheap, efficient, and effective production of pharmaceutical and therapeutic api's intermediates, and final products
WO2017139496A1 (en) 2016-02-09 2017-08-17 Cevolva Biotech, Inc. Microbial engineering for the production of cannabinoids and cannabinoid precursors
AU2018220470B2 (en) 2017-02-17 2022-02-24 Hyasynth Biologicals Inc. Method and cell line for production of polyketides in yeast
EP3615667B1 (en) * 2017-04-27 2021-08-11 The Regents of The University of California Microorganisms and methods for producing cannabinoids and cannabinoid derivatives
EP3652327A4 (en) * 2017-07-12 2021-04-21 Biomedican, Inc. Production of cannabinoids in yeast
EP3692143A4 (en) * 2017-10-05 2021-09-29 Eleszto Genetika, Inc. MICRO-ORGANISMS AND PROCESSES FOR THE FERMENTATION OF CANNABINOIDS

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