ES2877108T3 - Activación de linfocitos infiltrantes de médula en condiciones hipóxicas alternantes con normóxicas - Google Patents
Activación de linfocitos infiltrantes de médula en condiciones hipóxicas alternantes con normóxicas Download PDFInfo
- Publication number
- ES2877108T3 ES2877108T3 ES15838358T ES15838358T ES2877108T3 ES 2877108 T3 ES2877108 T3 ES 2877108T3 ES 15838358 T ES15838358 T ES 15838358T ES 15838358 T ES15838358 T ES 15838358T ES 2877108 T3 ES2877108 T3 ES 2877108T3
- Authority
- ES
- Spain
- Prior art keywords
- composition
- mil
- activated
- days
- population
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010021143 Hypoxia Diseases 0.000 title claims abstract description 101
- 230000001146 hypoxic effect Effects 0.000 title claims abstract description 74
- 210000004698 lymphocyte Anatomy 0.000 title claims abstract description 58
- 230000004913 activation Effects 0.000 title description 8
- 210000004027 cell Anatomy 0.000 claims abstract description 89
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 84
- 239000000203 mixture Substances 0.000 claims abstract description 82
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 80
- 239000001301 oxygen Substances 0.000 claims abstract description 80
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 57
- 238000000034 method Methods 0.000 claims abstract description 45
- 210000001185 bone marrow Anatomy 0.000 claims abstract description 30
- 230000007954 hypoxia Effects 0.000 claims abstract description 27
- 201000011510 cancer Diseases 0.000 claims abstract description 16
- 108010002350 Interleukin-2 Proteins 0.000 claims abstract description 14
- 230000007959 normoxia Effects 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 9
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 11
- 239000011324 bead Substances 0.000 claims description 11
- 208000034578 Multiple myelomas Diseases 0.000 claims description 7
- 238000011282 treatment Methods 0.000 claims description 4
- 206010006187 Breast cancer Diseases 0.000 claims description 3
- 208000026310 Breast neoplasm Diseases 0.000 claims description 3
- 230000001225 therapeutic effect Effects 0.000 claims description 3
- 208000020816 lung neoplasm Diseases 0.000 claims description 2
- 230000002489 hematologic effect Effects 0.000 claims 1
- 208000037841 lung tumor Diseases 0.000 claims 1
- 239000013255 MILs Substances 0.000 description 55
- 210000001744 T-lymphocyte Anatomy 0.000 description 40
- 102000017420 CD3 protein, epsilon/gamma/delta subunit Human genes 0.000 description 38
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 25
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 description 19
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 description 19
- 102100034922 T-cell surface glycoprotein CD8 alpha chain Human genes 0.000 description 17
- 230000012010 growth Effects 0.000 description 15
- 102100036011 T-cell surface glycoprotein CD4 Human genes 0.000 description 13
- 238000000684 flow cytometry Methods 0.000 description 13
- 238000011534 incubation Methods 0.000 description 11
- 102000000588 Interleukin-2 Human genes 0.000 description 10
- 238000001727 in vivo Methods 0.000 description 8
- 239000000427 antigen Substances 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 210000003171 tumor-infiltrating lymphocyte Anatomy 0.000 description 7
- 238000013459 approach Methods 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 102000004127 Cytokines Human genes 0.000 description 5
- 108090000695 Cytokines Proteins 0.000 description 5
- 101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 description 5
- 102000008070 Interferon-gamma Human genes 0.000 description 5
- 108010074328 Interferon-gamma Proteins 0.000 description 5
- 102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 238000002659 cell therapy Methods 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 229960003130 interferon gamma Drugs 0.000 description 5
- 208000002250 Hematologic Neoplasms Diseases 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 201000000050 myeloid neoplasm Diseases 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 239000011534 wash buffer Substances 0.000 description 4
- 101150106931 IFNG gene Proteins 0.000 description 3
- -1 antibodies Substances 0.000 description 3
- 210000000612 antigen-presenting cell Anatomy 0.000 description 3
- 238000002512 chemotherapy Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 210000003289 regulatory T cell Anatomy 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- 230000003827 upregulation Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 description 2
- 206010027480 Metastatic malignant melanoma Diseases 0.000 description 2
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000000735 allogeneic effect Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 230000005809 anti-tumor immunity Effects 0.000 description 2
- 230000006907 apoptotic process Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000001143 conditioned effect Effects 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 238000004163 cytometry Methods 0.000 description 2
- 230000001472 cytotoxic effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 238000009169 immunotherapy Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 208000021039 metastatic melanoma Diseases 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 239000003104 tissue culture media Substances 0.000 description 2
- 102100023990 60S ribosomal protein L17 Human genes 0.000 description 1
- 208000031261 Acute myeloid leukaemia Diseases 0.000 description 1
- 102100027207 CD27 antigen Human genes 0.000 description 1
- 108010021064 CTLA-4 Antigen Proteins 0.000 description 1
- 229940045513 CTLA4 antagonist Drugs 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 102100039498 Cytotoxic T-lymphocyte protein 4 Human genes 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101000914511 Homo sapiens CD27 antigen Proteins 0.000 description 1
- 101000998146 Homo sapiens Interleukin-17A Proteins 0.000 description 1
- 101001018097 Homo sapiens L-selectin Proteins 0.000 description 1
- 101000611023 Homo sapiens Tumor necrosis factor receptor superfamily member 6 Proteins 0.000 description 1
- 102100033461 Interleukin-17A Human genes 0.000 description 1
- 108010002586 Interleukin-7 Proteins 0.000 description 1
- 102100033467 L-selectin Human genes 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 206010025327 Lymphopenia Diseases 0.000 description 1
- 208000033776 Myeloid Acute Leukemia Diseases 0.000 description 1
- 101710089372 Programmed cell death protein 1 Proteins 0.000 description 1
- 102100040403 Tumor necrosis factor receptor superfamily member 6 Human genes 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001446 anti-myeloma Effects 0.000 description 1
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000002617 apheresis Methods 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 238000010322 bone marrow transplantation Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 208000035269 cancer or benign tumor Diseases 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000003021 clonogenic effect Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 229960004397 cyclophosphamide Drugs 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000009699 differential effect Effects 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000003284 homeostatic effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000008102 immune modulation Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 230000000527 lymphocytic effect Effects 0.000 description 1
- 231100001023 lymphopenia Toxicity 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000003071 memory t lymphocyte Anatomy 0.000 description 1
- 230000001400 myeloablative effect Effects 0.000 description 1
- 230000003039 myelosuppressive effect Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001272 nitrous oxide Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000011476 stem cell transplantation Methods 0.000 description 1
- 210000002536 stromal cell Anatomy 0.000 description 1
- 230000003614 tolerogenic effect Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/10—Cellular immunotherapy characterised by the cell type used
- A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
- A61K40/41—Vertebrate antigens
- A61K40/42—Cancer antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0669—Bone marrow stromal cells; Whole bone marrow
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2302—Interleukin-2 (IL-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Developmental Biology & Embryology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Rheumatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462045782P | 2014-09-04 | 2014-09-04 | |
| US201562186040P | 2015-06-29 | 2015-06-29 | |
| PCT/US2015/048536 WO2016037054A1 (en) | 2014-09-04 | 2015-09-04 | Activation of marrow infiltrating lymphocytes in hypoxic alternating with normoxic conditions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2877108T3 true ES2877108T3 (es) | 2021-11-16 |
Family
ID=55440392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES15838358T Active ES2877108T3 (es) | 2014-09-04 | 2015-09-04 | Activación de linfocitos infiltrantes de médula en condiciones hipóxicas alternantes con normóxicas |
Country Status (13)
| Country | Link |
|---|---|
| US (7) | US9687510B2 (https=) |
| EP (2) | EP3922254A1 (https=) |
| JP (2) | JP6865160B2 (https=) |
| KR (1) | KR20170067751A (https=) |
| CN (1) | CN107109364A (https=) |
| AU (2) | AU2015311761B2 (https=) |
| CA (1) | CA2959994C (https=) |
| DK (1) | DK3188740T3 (https=) |
| ES (1) | ES2877108T3 (https=) |
| IL (2) | IL250746B (https=) |
| MX (1) | MX391017B (https=) |
| PT (1) | PT3188740T (https=) |
| WO (1) | WO2016037054A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015157636A1 (en) | 2014-04-10 | 2015-10-15 | H. Lee Moffitt Cancer Center And Research Institute, Inc. | Enhanced expansion of tumor-infiltrating lymphocytes for adoptive cell therapy |
| US9687510B2 (en) * | 2014-09-04 | 2017-06-27 | The Johns Hopkins University | Activation of marrow infiltrating lymphocytes in hypoxic alternating with normoxic conditions |
| CA2991040A1 (en) * | 2015-06-29 | 2017-01-05 | The Johns Hopkins University | Immune checkpoint chimeric receptors therapy |
| EP3320087A4 (en) * | 2015-07-08 | 2019-01-23 | The Johns Hopkins University | BONE MARROW FILTRATION LYMPHOCYTES (MILS) AS A T-CELL SOURCE FOR CHIMERIC ANTIGEN RECEPTOR (CAR) THERAPY |
| MX2019004707A (es) | 2016-10-26 | 2019-08-12 | Iovance Biotherapeutics Inc | Reestimulacion de linfocitos infiltrantes de tumor crioconservados. |
| TWI788307B (zh) | 2016-10-31 | 2023-01-01 | 美商艾歐凡斯生物治療公司 | 用於擴增腫瘤浸潤性淋巴細胞之工程化人造抗原呈現細胞 |
| US11401507B2 (en) | 2016-11-17 | 2022-08-02 | Iovance Biotherapeutics, Inc. | Remnant tumor infiltrating lymphocytes and methods of preparing and using the same |
| JP2020512284A (ja) * | 2016-12-22 | 2020-04-23 | ウィンドミル セラピューティクス インコーポレイテッド | 免疫系調節組成物および方法 |
| CA3049165A1 (en) | 2017-01-06 | 2018-07-12 | Iovance Biotherapeutics, Inc. | Expansion of tumor infiltrating lymphocytes with potassium channel agonists and therapeutic uses thereof |
| JOP20190224A1 (ar) | 2017-03-29 | 2019-09-26 | Iovance Biotherapeutics Inc | عمليات من أجل إنتاج الخلايا اللمفاوية المرتشحة للأورام واستخداماتها في العلاج المناعي |
| US11254913B1 (en) | 2017-03-29 | 2022-02-22 | Iovance Biotherapeutics, Inc. | Processes for production of tumor infiltrating lymphocytes and uses of same in immunotherapy |
| SG11202006541UA (en) | 2018-01-08 | 2020-08-28 | Iovance Biotherapeutics Inc | Processes for generating til products enriched for tumor antigen-specific t-cells |
| EP3735256A4 (en) | 2018-03-22 | 2021-10-13 | Windmil Therapeutics, Inc. | BONE MARROW LYMPHOCYTES SPECIFIC TO PROSTATE CANCER AND ASSOCIATED USES |
| WO2019217753A1 (en) | 2018-05-10 | 2019-11-14 | Iovance Biotherapeutics, Inc. | Processes for production of tumor infiltrating lymphocytes and uses of same in immunotherapy |
| JP2022507113A (ja) * | 2018-11-05 | 2022-01-18 | ウィンドミル セラピューティクス インコーポレイテッド | クローン性の増大した骨髄浸潤リンパ球およびそれらの使用 |
| TW202039830A (zh) | 2018-11-05 | 2020-11-01 | 美商艾歐凡斯生物治療公司 | 用於製造腫瘤浸潤性淋巴細胞之方法及其在免疫療法中之用途 |
| SG11202104637VA (en) * | 2018-11-30 | 2021-06-29 | Windmil Therapeutics Inc | MARROW INFILTRATING LYMPHOCYTES (MILs) EXPRESSING CHIMERIC ANTIGEN RECEPTORS (CAR), METHOD OF MANUFACTURING SAME, AND METHOD OF USING IN THERAPY |
| WO2020198031A1 (en) * | 2019-03-22 | 2020-10-01 | Windmil Therapeutics Inc. | Lung cancer specific marrow infiltrating lymphocytes and uses thereof |
| US20230100744A1 (en) * | 2020-02-28 | 2023-03-30 | Windmil Therapeutics, Inc. | Methods for enriching marrow infiltrating lymphocytes ("mils"), compositions containing enriched mils, and methods of using enriched mils |
| WO2022066872A1 (en) * | 2020-09-24 | 2022-03-31 | Windmil Therapeutics, Inc. | Marrow infiltrating lymphocytes (mils) expressing t cell receptors, methods of manufacturing same, and method of using in therapy |
| US20230372397A1 (en) | 2020-10-06 | 2023-11-23 | Iovance Biotherapeutics, Inc. | Treatment of nsclc patients with tumor infiltrating lymphocyte therapies |
| AU2022246174A1 (en) | 2021-03-25 | 2023-09-14 | Iovance Biotherapeutics, Inc. | Methods and compositions for t-cell coculture potency assays and use with cell therapy products |
| CA3248034A1 (en) | 2022-04-15 | 2023-10-19 | Iovance Biotherapeutics, Inc. | METHODS FOR EXPANSION OF TIL CELLS BY MEANS OF SPECIFIC CYTOKINE COMBINATIONS AND/OR AKT INHIBITOR TREATMENT |
| WO2025160242A1 (en) * | 2024-01-23 | 2025-07-31 | H. Lee Moffitt Cancer Center And Research Institute, Inc. | Method of generating tils from cerebro spinal fluid for leptomeningeal disease |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3243600A (en) | 1999-02-23 | 2000-09-14 | Kathleen S. Carswell | Improved method of culturing t cells |
| US6811788B2 (en) * | 2000-01-19 | 2004-11-02 | Baofa Yu | Combinations and methods for treating neoplasms |
| WO2008147482A2 (en) * | 2007-02-13 | 2008-12-04 | Northeastern University | Methods and compositions for improving immune responses |
| WO2010062742A2 (en) * | 2008-11-03 | 2010-06-03 | The Johns Hopkins University | Methods for freparation and use of marrow infiltrating lymphocytes (mils) |
| WO2014085437A2 (en) * | 2012-11-27 | 2014-06-05 | The Johns Hopkins University | Use of post-transplant cyclophosphamide treated allogeneic marrow infiltrating lymphocytes to augment anti-tumor immunity |
| BR112014007947A2 (pt) | 2011-10-03 | 2017-04-18 | Univ Los Andes | método para monitoramento, diagnóstico e/ou prognóstico de distúrbios relacionados à hipoxia usando nfat5 |
| WO2013109759A1 (en) * | 2012-01-17 | 2013-07-25 | Northeastern University | Methods and compositions for expanding immunosuppressive t regulatory cells in vitro and uses thereof |
| JP2014090678A (ja) * | 2012-11-01 | 2014-05-19 | Ito En Ltd | 容器詰野菜汁及び／又は果汁含有飲料及びその製造方法、並びに容器詰野菜汁及び／又は果汁含有飲料の呈味劣化防止方法 |
| US9687510B2 (en) | 2014-09-04 | 2017-06-27 | The Johns Hopkins University | Activation of marrow infiltrating lymphocytes in hypoxic alternating with normoxic conditions |
| JP2020512284A (ja) * | 2016-12-22 | 2020-04-23 | ウィンドミル セラピューティクス インコーポレイテッド | 免疫系調節組成物および方法 |
| EP3735256A4 (en) * | 2018-03-22 | 2021-10-13 | Windmil Therapeutics, Inc. | BONE MARROW LYMPHOCYTES SPECIFIC TO PROSTATE CANCER AND ASSOCIATED USES |
| SG11202104637VA (en) * | 2018-11-30 | 2021-06-29 | Windmil Therapeutics Inc | MARROW INFILTRATING LYMPHOCYTES (MILs) EXPRESSING CHIMERIC ANTIGEN RECEPTORS (CAR), METHOD OF MANUFACTURING SAME, AND METHOD OF USING IN THERAPY |
-
2015
- 2015-09-04 US US15/112,353 patent/US9687510B2/en active Active
- 2015-09-04 EP EP21167891.7A patent/EP3922254A1/en not_active Withdrawn
- 2015-09-04 WO PCT/US2015/048536 patent/WO2016037054A1/en not_active Ceased
- 2015-09-04 DK DK15838358.8T patent/DK3188740T3/da active
- 2015-09-04 EP EP15838358.8A patent/EP3188740B1/en active Active
- 2015-09-04 PT PT158383588T patent/PT3188740T/pt unknown
- 2015-09-04 MX MX2017002852A patent/MX391017B/es unknown
- 2015-09-04 ES ES15838358T patent/ES2877108T3/es active Active
- 2015-09-04 JP JP2017512293A patent/JP6865160B2/ja not_active Expired - Fee Related
- 2015-09-04 CA CA2959994A patent/CA2959994C/en active Active
- 2015-09-04 CN CN201580059448.3A patent/CN107109364A/zh active Pending
- 2015-09-04 AU AU2015311761A patent/AU2015311761B2/en not_active Ceased
- 2015-09-04 KR KR1020177009084A patent/KR20170067751A/ko not_active Abandoned
-
2017
- 2017-02-23 IL IL250746A patent/IL250746B/en active IP Right Grant
- 2017-05-26 US US15/606,766 patent/US10406178B2/en active Active
- 2017-05-26 US US15/606,951 patent/US10172887B2/en active Active
-
2018
- 2018-12-03 US US16/207,864 patent/US11160830B2/en not_active Expired - Fee Related
-
2019
- 2019-07-30 US US16/526,656 patent/US11406666B2/en active Active
-
2020
- 2020-07-28 IL IL276347A patent/IL276347B/en unknown
-
2021
- 2021-02-02 AU AU2021200648A patent/AU2021200648A1/en not_active Abandoned
- 2021-04-05 JP JP2021063957A patent/JP7197213B2/ja active Active
- 2021-09-30 US US17/490,691 patent/US20220016172A1/en not_active Abandoned
-
2022
- 2022-07-14 US US17/864,546 patent/US20230000919A1/en active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2877108T3 (es) | Activación de linfocitos infiltrantes de médula en condiciones hipóxicas alternantes con normóxicas | |
| JP6010136B2 (ja) | ナチュラルキラー細胞の製造方法、その方法により製造されたナチュラルキラー細胞、並びにそれを含む腫瘍及び感染性疾患治療用組成物 | |
| ES2832608T3 (es) | Método de expansión de linfocitos citolíticos naturales | |
| US12011459B2 (en) | Methods for manufacturing T cells by direct sorting and compositions thereof | |
| AU2006288348B2 (en) | Method for activation treatment of antigen-presenting cell | |
| CA3094610A1 (en) | Method for producing natural killer cells | |
| JP4953403B2 (ja) | がん免疫療法用細胞の製造方法 | |
| AU2003260473B2 (en) | Use of dendritic cells (DCs) expressing interleukin 12 (IL-12) | |
| HK40064965A (en) | Activation of marrow infiltrating lymphocytes in hypoxic alternating with normoxic conditions | |
| HK1240085B (en) | Activation of marrow infiltrating lymphocytes in hypoxic alternating with normoxic conditions | |
| HK1240085A1 (en) | Activation of marrow infiltrating lymphocytes in hypoxic alternating with normoxic conditions | |
| HK1240616A1 (en) | Activation of marrow infiltrating lymphocytes in hypoxic alternating with normoxic conditions |