ES2865124T3 - Procedimiento en una etapa para la purificación de ácidos nuncleicos - Google Patents

Procedimiento en una etapa para la purificación de ácidos nuncleicos Download PDF

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Publication number
ES2865124T3
ES2865124T3 ES14765295T ES14765295T ES2865124T3 ES 2865124 T3 ES2865124 T3 ES 2865124T3 ES 14765295 T ES14765295 T ES 14765295T ES 14765295 T ES14765295 T ES 14765295T ES 2865124 T3 ES2865124 T3 ES 2865124T3
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nucleic acids
sample
solid substrate
gel
nucleic acid
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English (en)
Spanish (es)
Inventor
Gerard Gundling
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Abbott Molecular Inc
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Abbott Molecular Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Virology (AREA)
ES14765295T 2013-03-15 2014-03-14 Procedimiento en una etapa para la purificación de ácidos nuncleicos Active ES2865124T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361789622P 2013-03-15 2013-03-15
PCT/US2014/028518 WO2014144209A1 (en) 2013-03-15 2014-03-14 One-step procedure for the purification of nucleic acids

Publications (1)

Publication Number Publication Date
ES2865124T3 true ES2865124T3 (es) 2021-10-15

Family

ID=51528702

Family Applications (1)

Application Number Title Priority Date Filing Date
ES14765295T Active ES2865124T3 (es) 2013-03-15 2014-03-14 Procedimiento en una etapa para la purificación de ácidos nuncleicos

Country Status (6)

Country Link
US (2) US9803230B2 (enExample)
EP (2) EP2969140B1 (enExample)
JP (2) JP6431522B2 (enExample)
CN (1) CN105120986B (enExample)
ES (1) ES2865124T3 (enExample)
WO (1) WO2014144209A1 (enExample)

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CN108350448A (zh) * 2015-09-10 2018-07-31 株式会社钟化 从含有核酸的样品中分离核酸的方法及用于该方法的装置
CA3036932A1 (en) * 2016-10-04 2018-04-12 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
US11732254B2 (en) 2017-06-30 2023-08-22 Pacific Biosicences of California, Inc. Size selection purification using a thermoplastic silica nanomaterial
KR20250020281A (ko) * 2021-05-06 2025-02-11 애보트 모레큘러 인크. 간단한 샘플 추출을 위한 조성물 및 방법
US20220372465A1 (en) * 2021-05-20 2022-11-24 Kryptos Biotechnologies, Inc. Methods and systems for extracting nucleic acids from a biological sample
US20230080090A1 (en) * 2021-08-30 2023-03-16 Abbott Molecular Inc. Selective purification of rna
CN121219073A (zh) * 2023-05-16 2025-12-26 阿提拉生物系统公司 反应容器中的可穿透凝胶

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Also Published As

Publication number Publication date
US9803230B2 (en) 2017-10-31
JP2016514453A (ja) 2016-05-23
JP2018198599A (ja) 2018-12-20
WO2014144209A1 (en) 2014-09-18
US20140272944A1 (en) 2014-09-18
WO2014144209A4 (en) 2014-11-20
CN105120986B (zh) 2019-03-12
EP2969140A1 (en) 2016-01-20
JP6684868B2 (ja) 2020-04-22
WO2014144209A8 (en) 2014-10-16
CN105120986A (zh) 2015-12-02
JP6431522B2 (ja) 2018-11-28
US20180010168A1 (en) 2018-01-11
EP3828284A1 (en) 2021-06-02
EP2969140A4 (en) 2017-05-03
EP2969140B1 (en) 2021-02-17

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