ES2865124T3 - Procedimiento en una etapa para la purificación de ácidos nuncleicos - Google Patents
Procedimiento en una etapa para la purificación de ácidos nuncleicos Download PDFInfo
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- ES2865124T3 ES2865124T3 ES14765295T ES14765295T ES2865124T3 ES 2865124 T3 ES2865124 T3 ES 2865124T3 ES 14765295 T ES14765295 T ES 14765295T ES 14765295 T ES14765295 T ES 14765295T ES 2865124 T3 ES2865124 T3 ES 2865124T3
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- nucleic acids
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- solid substrate
- gel
- nucleic acid
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Virology (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361789622P | 2013-03-15 | 2013-03-15 | |
| PCT/US2014/028518 WO2014144209A1 (en) | 2013-03-15 | 2014-03-14 | One-step procedure for the purification of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2865124T3 true ES2865124T3 (es) | 2021-10-15 |
Family
ID=51528702
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES14765295T Active ES2865124T3 (es) | 2013-03-15 | 2014-03-14 | Procedimiento en una etapa para la purificación de ácidos nuncleicos |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9803230B2 (enExample) |
| EP (2) | EP3828284A1 (enExample) |
| JP (2) | JP6431522B2 (enExample) |
| CN (1) | CN105120986B (enExample) |
| ES (1) | ES2865124T3 (enExample) |
| WO (1) | WO2014144209A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014144209A1 (en) * | 2013-03-15 | 2014-09-18 | Abbott Molecular Inc. | One-step procedure for the purification of nucleic acids |
| EP3322805B1 (en) | 2015-07-14 | 2021-10-20 | Abbott Molecular Inc. | Purification of nucleic acids using copper-titanium oxides or magnesium-titanium oxides |
| WO2017043649A1 (ja) * | 2015-09-10 | 2017-03-16 | 株式会社カネカ | 核酸を含有する検体から核酸を分離する方法及びそのための装置 |
| WO2018067736A1 (en) * | 2016-10-04 | 2018-04-12 | Sage Science, Inc. | Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation |
| JP7136817B2 (ja) | 2017-06-30 | 2022-09-13 | サーキュロミクス インク | 熱可塑性シリカナノ材料を使用するサイズ選択精製 |
| EP4334469A4 (en) * | 2021-05-06 | 2025-01-29 | Abbott Molecular Inc. | COMPOSITIONS AND METHODS OF COLLECTION OF SINGLE SAMPLES |
| US20220372465A1 (en) * | 2021-05-20 | 2022-11-24 | Kryptos Biotechnologies, Inc. | Methods and systems for extracting nucleic acids from a biological sample |
| US20230080090A1 (en) * | 2021-08-30 | 2023-03-16 | Abbott Molecular Inc. | Selective purification of rna |
| WO2024238645A2 (en) * | 2023-05-16 | 2024-11-21 | Atila Biosystems Incorporated | Penetrable gel in a reaction vessel |
Family Cites Families (67)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI844027A7 (fi) | 1984-10-12 | 1986-04-13 | Labsystems Oy | Immunologiskt bestaemningsfoerfarande. |
| FI850481A0 (fi) | 1985-02-06 | 1985-02-06 | Labsystems Oy | Foerfarande foer bestaemning av motmedel eller antigener. |
| JP2591738B2 (ja) | 1985-08-21 | 1997-03-19 | ベーリンガー マンヘイム コーポレイション | 特異的結合アッセイにおける成分の分離,混合および検出のための方法および装置 |
| ATE91023T1 (de) | 1987-12-01 | 1993-07-15 | Biotope Inc | Verfahren und vorrichtungen zur durchfuehrung von untersuchungen. |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US5279936A (en) | 1989-12-22 | 1994-01-18 | Syntex (U.S.A.) Inc. | Method of separation employing magnetic particles and second medium |
| US5465344A (en) * | 1990-08-20 | 1995-11-07 | Matsushita Electric Industrial Co., Ltd. | Microprocessor with dual-port cache memory for reducing penalty of consecutive memory address accesses |
| WO1994026414A1 (en) | 1993-05-17 | 1994-11-24 | Syntex (U.S.A.) Inc. | Reaction container for specific binding assays and method for its use |
| US5637687A (en) * | 1993-08-31 | 1997-06-10 | Wiggins; James C. | Methods and compositions for isolating nucleic acids |
| US5455344A (en) | 1993-09-03 | 1995-10-03 | Fmc Corporation | Agarose compositions for nucleic acid sequencing |
| DK0743950T3 (da) * | 1994-02-11 | 2002-03-25 | Qiagen Gmbh | Fremgangsmåde til adskillelse af dobbeltstrengede/enkeltstrengede nukleinsyre-strukturer |
| US5646263A (en) | 1994-09-19 | 1997-07-08 | Promega Corporation | High efficiency method for isolating target substances using a multisample separation device |
| US5705628A (en) * | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5580655A (en) * | 1995-03-03 | 1996-12-03 | Dow Corning Corporation | Silica nanoparticles |
| DE19512368A1 (de) | 1995-04-01 | 1996-10-02 | Boehringer Mannheim Gmbh | System zur Freisetzung und Isolierung von Nukleinsäuren |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| EP1712921A2 (en) | 1997-09-29 | 2006-10-18 | F.Hoffmann-La Roche Ag | Apparatus for separating magnetic particles |
| DE19746874A1 (de) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
| US6355470B1 (en) * | 1999-09-03 | 2002-03-12 | E. I. Du Pont De Nemours And Company | Genes encoding picric acid degradation |
| SE9904539D0 (sv) * | 1999-12-10 | 1999-12-10 | Alphahelix Ab | Method and device for the handling of samples and reagents |
| US7183002B2 (en) | 2000-03-24 | 2007-02-27 | Qiagen, Gmbh | Porous ferro- or ferrimagnetic glass particles for isolating molecules |
| GB0013658D0 (en) | 2000-06-05 | 2000-07-26 | Dynal Asa | Nucleic acid isolation |
| US6413756B2 (en) * | 2000-06-06 | 2002-07-02 | Pe Corporation (Ny) | Isolated human kinase proteins, nucleic acid molecules encoding human kinase proteins, and uses thereof |
| US6992782B1 (en) * | 2000-09-13 | 2006-01-31 | Canon Kabushiki Kaisha | Scalable vector graphics print driver |
| SG96587A1 (en) * | 2000-11-21 | 2003-06-16 | Nat Inst Of Education Nanyang | Production of stilbenes in transgenic plants and the method of producing thereof |
| AU2002311859A1 (en) * | 2001-04-26 | 2002-11-11 | Vanderbilt University | Compositions and methods for treating colorectal polyps and cancer |
| AU2002330668B2 (en) * | 2001-06-29 | 2007-11-15 | Nanomics Biosystems Pty, Ltd. | Synthesis and use of organosilica particles |
| WO2003030879A1 (en) * | 2001-10-05 | 2003-04-17 | Surmodics, Inc. | Particle immobilized coatings and uses thereof |
| US8110351B2 (en) * | 2002-01-16 | 2012-02-07 | Invitrogen Dynal As | Method for isolating nucleic acids and protein from a single sample |
| JP4080798B2 (ja) | 2002-06-27 | 2008-04-23 | 日立マクセル株式会社 | 核酸結合用磁性担体およびその製造方法 |
| CA2497420C (en) * | 2002-09-03 | 2016-04-19 | Quanta Biosciences, Inc. | Improved compositions and methods for cdna synthesis |
| US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| US7025864B2 (en) * | 2003-03-10 | 2006-04-11 | Elchrom Scientific A.G. | Method and apparatus for recovering target molecules from a gel containing said target molecules |
| FR2855822B1 (fr) * | 2003-06-05 | 2005-07-22 | Univ Grenoble 1 | Acides nucleiques en tant que nouveaux selecteurs chiraux specifiques |
| DE10331107B3 (de) * | 2003-07-04 | 2004-12-02 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in DNA mittels Cytidin-Deaminasen |
| US20050059024A1 (en) * | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| EP1510577A1 (en) | 2003-08-29 | 2005-03-02 | Qiagen GmbH | Method for magnetic bead isolation of nucleic acids |
| US20050208037A1 (en) * | 2003-10-21 | 2005-09-22 | Board Of Regents, The University Of Texas System | Thioredoxin increases redox-cycling of anticancer agents thereby sensitizes cancer cells to apoptosis |
| US7947345B2 (en) * | 2003-11-07 | 2011-05-24 | Hewlett-Packard Development Company, L.P. | Synthesis of poly(ethylene amine) on an oxide support |
| US20050106577A1 (en) * | 2003-11-17 | 2005-05-19 | Hashem Akhavan-Tafti | Cleavable solid phases for isolating nucleic acids |
| ATE471212T1 (de) | 2004-01-23 | 2010-07-15 | Qiagen Gmbh | Kontaminationsbarriere |
| US8426126B2 (en) * | 2004-03-18 | 2013-04-23 | Applied Biosystems, Llc | Modified surfaces as solid supports for nucleic acid purification |
| US20050287593A1 (en) * | 2004-05-03 | 2005-12-29 | Schering Corporation | Use of cytokine expression to predict skin inflammation; methods of treatment |
| CA2592204C (en) | 2004-12-23 | 2013-03-12 | I-Stat Corporation | Nucleic acid diagnostics system and methods |
| CN101331148B (zh) * | 2005-12-14 | 2012-06-27 | 霍夫曼-拉罗奇有限公司 | 用于亚硫酸氢盐处理的新方法 |
| US7799534B2 (en) * | 2006-05-09 | 2010-09-21 | Beckman Coulter, Inc. | Nonseparation assay methods |
| WO2008051985A2 (en) * | 2006-10-23 | 2008-05-02 | Oregon Health & Science University | Method for separation and identification of biomolecules using unconventional gel electrophoresis and detection of single nanoparticle probes |
| WO2009020609A2 (en) * | 2007-08-06 | 2009-02-12 | Nanogen, Inc. | Isolation of nucleic acids molecules using modified solid supports |
| WO2009117167A1 (en) * | 2008-01-02 | 2009-09-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| US8669079B2 (en) * | 2008-11-12 | 2014-03-11 | Cara Therapeutics, Inc. | Methods for genetic analysis of textiles made of Gossypium barbadense and Gossypium hirsutum cotton |
| JP2012511156A (ja) * | 2008-12-05 | 2012-05-17 | ナノアイヴイディー,インコーポレイテッド | ポイント・オブ・ケア分析器用マイクロ流体ベースのラボオンテストカード |
| DE102008061714A1 (de) * | 2008-12-12 | 2010-06-17 | Siemens Healthcare Diagnostics Inc., Deerfield | Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe |
| US20110039267A1 (en) * | 2009-08-13 | 2011-02-17 | Cha-Mei Tang | Sample Preparation And Detection Of Analytes Using Silica Beads |
| US20110097723A1 (en) * | 2009-09-19 | 2011-04-28 | Qun Liu | Methods and reagents for analyte detection |
| US8536322B2 (en) * | 2009-10-19 | 2013-09-17 | Zhiqiang Han | Method for nucleic acid isolation by solid phase reversible binding of nucleic acids |
| FR2952368B1 (fr) * | 2009-11-10 | 2012-01-13 | Commissariat Energie Atomique | Particule de silice incorporant une molecule d'interet, son procede de preparation et ses utilisations. |
| JP5528231B2 (ja) * | 2010-06-28 | 2014-06-25 | キヤノン株式会社 | 画像形成装置 |
| WO2012061726A2 (en) | 2010-11-05 | 2012-05-10 | University Of Utah Research Foundation | Compositions and methods for sequencing dna |
| US9517472B2 (en) * | 2010-12-21 | 2016-12-13 | Shimadzu Corporation | Device and method for processing target component in tube |
| US20120208026A1 (en) * | 2011-02-10 | 2012-08-16 | Xerox Corporation | Silica-Coated Magnetic Nanoparticles and Process for Making Same |
| US20150122652A1 (en) | 2011-07-19 | 2015-05-07 | Tennessee Technological University | Nanocomposite polymer hydrogel with aligned nanoparticles |
| US9353409B2 (en) * | 2013-01-15 | 2016-05-31 | Jun Euihum Lee | Compositions and methods for RT-PCR |
| WO2014144209A1 (en) * | 2013-03-15 | 2014-09-18 | Abbott Molecular Inc. | One-step procedure for the purification of nucleic acids |
| US8734364B1 (en) * | 2013-11-07 | 2014-05-27 | Genetic Technologies Limited | Device and method for obtaining a biological sample |
-
2014
- 2014-03-14 WO PCT/US2014/028518 patent/WO2014144209A1/en not_active Ceased
- 2014-03-14 ES ES14765295T patent/ES2865124T3/es active Active
- 2014-03-14 US US14/212,880 patent/US9803230B2/en active Active
- 2014-03-14 EP EP20216686.4A patent/EP3828284A1/en not_active Withdrawn
- 2014-03-14 JP JP2016502811A patent/JP6431522B2/ja not_active Expired - Fee Related
- 2014-03-14 EP EP14765295.2A patent/EP2969140B1/en active Active
- 2014-03-14 CN CN201480014153.XA patent/CN105120986B/zh not_active Expired - Fee Related
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2018
- 2018-07-19 JP JP2018135643A patent/JP6684868B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP2969140A1 (en) | 2016-01-20 |
| WO2014144209A4 (en) | 2014-11-20 |
| EP2969140A4 (en) | 2017-05-03 |
| CN105120986B (zh) | 2019-03-12 |
| CN105120986A (zh) | 2015-12-02 |
| WO2014144209A1 (en) | 2014-09-18 |
| EP3828284A1 (en) | 2021-06-02 |
| US20140272944A1 (en) | 2014-09-18 |
| EP2969140B1 (en) | 2021-02-17 |
| JP6684868B2 (ja) | 2020-04-22 |
| JP2016514453A (ja) | 2016-05-23 |
| WO2014144209A8 (en) | 2014-10-16 |
| US20180010168A1 (en) | 2018-01-11 |
| US9803230B2 (en) | 2017-10-31 |
| JP6431522B2 (ja) | 2018-11-28 |
| JP2018198599A (ja) | 2018-12-20 |
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