CN105120986B - 用于核酸纯化的一步程序 - Google Patents

用于核酸纯化的一步程序 Download PDF

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Publication number
CN105120986B
CN105120986B CN201480014153.XA CN201480014153A CN105120986B CN 105120986 B CN105120986 B CN 105120986B CN 201480014153 A CN201480014153 A CN 201480014153A CN 105120986 B CN105120986 B CN 105120986B
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nucleic acid
sample
solid substrate
particle
gel
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CN105120986A (zh
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G.J.冈林
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Abbott Laboratories
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Abbott Laboratories
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Virology (AREA)
CN201480014153.XA 2013-03-15 2014-03-14 用于核酸纯化的一步程序 Expired - Fee Related CN105120986B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361789622P 2013-03-15 2013-03-15
US61/789622 2013-03-15
PCT/US2014/028518 WO2014144209A1 (en) 2013-03-15 2014-03-14 One-step procedure for the purification of nucleic acids

Publications (2)

Publication Number Publication Date
CN105120986A CN105120986A (zh) 2015-12-02
CN105120986B true CN105120986B (zh) 2019-03-12

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US (2) US9803230B2 (enExample)
EP (2) EP3828284A1 (enExample)
JP (2) JP6431522B2 (enExample)
CN (1) CN105120986B (enExample)
ES (1) ES2865124T3 (enExample)
WO (1) WO2014144209A1 (enExample)

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EP3322805B1 (en) 2015-07-14 2021-10-20 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides or magnesium-titanium oxides
WO2017043649A1 (ja) * 2015-09-10 2017-03-16 株式会社カネカ 核酸を含有する検体から核酸を分離する方法及びそのための装置
WO2018067736A1 (en) * 2016-10-04 2018-04-12 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
JP7136817B2 (ja) 2017-06-30 2022-09-13 サーキュロミクス インク 熱可塑性シリカナノ材料を使用するサイズ選択精製
EP4334469A4 (en) * 2021-05-06 2025-01-29 Abbott Molecular Inc. COMPOSITIONS AND METHODS OF COLLECTION OF SINGLE SAMPLES
US20220372465A1 (en) * 2021-05-20 2022-11-24 Kryptos Biotechnologies, Inc. Methods and systems for extracting nucleic acids from a biological sample
US20230080090A1 (en) * 2021-08-30 2023-03-16 Abbott Molecular Inc. Selective purification of rna
WO2024238645A2 (en) * 2023-05-16 2024-11-21 Atila Biosystems Incorporated Penetrable gel in a reaction vessel

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CN1617938A (zh) * 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法

Also Published As

Publication number Publication date
EP2969140A1 (en) 2016-01-20
WO2014144209A4 (en) 2014-11-20
EP2969140A4 (en) 2017-05-03
CN105120986A (zh) 2015-12-02
WO2014144209A1 (en) 2014-09-18
EP3828284A1 (en) 2021-06-02
US20140272944A1 (en) 2014-09-18
EP2969140B1 (en) 2021-02-17
JP6684868B2 (ja) 2020-04-22
JP2016514453A (ja) 2016-05-23
WO2014144209A8 (en) 2014-10-16
US20180010168A1 (en) 2018-01-11
US9803230B2 (en) 2017-10-31
JP6431522B2 (ja) 2018-11-28
JP2018198599A (ja) 2018-12-20
ES2865124T3 (es) 2021-10-15

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