ES2811518T3 - Preparación de bibliotecas de ácido nucleico marcado usando protocolo de adición en un solo tubo - Google Patents
Preparación de bibliotecas de ácido nucleico marcado usando protocolo de adición en un solo tubo Download PDFInfo
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- ES2811518T3 ES2811518T3 ES15744405T ES15744405T ES2811518T3 ES 2811518 T3 ES2811518 T3 ES 2811518T3 ES 15744405 T ES15744405 T ES 15744405T ES 15744405 T ES15744405 T ES 15744405T ES 2811518 T3 ES2811518 T3 ES 2811518T3
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- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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| CN106795651A (zh) | 2017-05-31 |
| CA2953367A1 (en) | 2015-12-30 |
| AU2015279862B2 (en) | 2021-07-15 |
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