ES2778223T3 - Aislamiento de ARNmicro de fluido biológico - Google Patents

Aislamiento de ARNmicro de fluido biológico Download PDF

Info

Publication number
ES2778223T3
ES2778223T3 ES14872184T ES14872184T ES2778223T3 ES 2778223 T3 ES2778223 T3 ES 2778223T3 ES 14872184 T ES14872184 T ES 14872184T ES 14872184 T ES14872184 T ES 14872184T ES 2778223 T3 ES2778223 T3 ES 2778223T3
Authority
ES
Spain
Prior art keywords
mirna
biological fluid
sodium
surfactant
binding protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES14872184T
Other languages
English (en)
Spanish (es)
Inventor
Carol Kreader
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Sigma Aldrich Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co LLC filed Critical Sigma Aldrich Co LLC
Application granted granted Critical
Publication of ES2778223T3 publication Critical patent/ES2778223T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21014—Microbial serine proteases (3.4.21.14)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
ES14872184T 2013-11-27 2014-11-25 Aislamiento de ARNmicro de fluido biológico Active ES2778223T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361909834P 2013-11-27 2013-11-27
US201461985000P 2014-04-28 2014-04-28
PCT/US2014/067321 WO2015094609A1 (en) 2013-11-27 2014-11-25 Micro rna isolation from biological fluid

Publications (1)

Publication Number Publication Date
ES2778223T3 true ES2778223T3 (es) 2020-08-10

Family

ID=53403495

Family Applications (2)

Application Number Title Priority Date Filing Date
ES20152748T Active ES2930344T3 (es) 2013-11-27 2014-11-25 Aislamiento de micro ARN a partir de fluido biológico
ES14872184T Active ES2778223T3 (es) 2013-11-27 2014-11-25 Aislamiento de ARNmicro de fluido biológico

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES20152748T Active ES2930344T3 (es) 2013-11-27 2014-11-25 Aislamiento de micro ARN a partir de fluido biológico

Country Status (10)

Country Link
US (2) US10131902B2 (enExample)
EP (2) EP3074413B1 (enExample)
JP (1) JP6644686B2 (enExample)
KR (1) KR102181103B1 (enExample)
CN (2) CN105934439B (enExample)
BR (1) BR112016011770B1 (enExample)
CA (1) CA2930704C (enExample)
ES (2) ES2930344T3 (enExample)
IL (1) IL245646B (enExample)
WO (1) WO2015094609A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105934439B (zh) 2013-11-27 2019-09-10 西格马-奥尔德里奇有限责任公司 从生物流体中分离微小rna
CN107385014B (zh) * 2017-06-13 2020-12-22 深圳大学 一种直接定量检测循环miRNA的RT-qPCR方法
KR102003594B1 (ko) 2017-08-08 2019-07-24 숙명여자대학교산학협력단 염증 반응 매개 신혈관생성 질환 진단용 조성물
CN111278844B (zh) 2017-09-13 2023-12-19 贝克顿·迪金森公司 用于使用氧化铁颗粒提取核酸的方法和组合物
ES3039888T3 (en) * 2018-03-22 2025-10-27 Idexx Lab Inc Methods for measuring bacteria in biological samples
CN109490533B (zh) * 2018-12-29 2022-02-15 南京澳林生物科技有限公司 一种胃蛋白酶原ⅱ(pgⅱ)检测试剂盒及生产工艺
EP4232574A4 (en) * 2020-10-26 2024-12-18 Eclipse Bioinnovations, Inc. METHODS AND KITS FOR THE ENRICHMENT OF POLYNUCLEOTIDES
US11795500B2 (en) 2021-08-19 2023-10-24 Eclipse Bioinnovations, Inc. Methods for detecting RNA binding protein complexes
CN113980954A (zh) * 2021-11-11 2022-01-28 德州学院 一种病毒rna保护剂及其制备方法与应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050142609A1 (en) * 2003-10-08 2005-06-30 Ebioscience Native immunoglobulin binding reagents and methods for making and using same
JP5349838B2 (ja) * 2007-11-30 2013-11-20 和光純薬工業株式会社 ｓｍａｌｌＲＮＡの取得用担体、取得方法及び取得用試薬
WO2010014636A1 (en) * 2008-07-28 2010-02-04 Rockefeller University Methods for identifying rna segments bound by rna-binding proteins or ribonucleoprotein complexes
US8846314B2 (en) * 2009-03-03 2014-09-30 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoretic focusing of nucleic acids
US20130197206A1 (en) * 2010-09-24 2013-08-01 Wako Pure Chemical Industries, Ltd. Method for acquisition of small rna
AU2012220872A1 (en) * 2011-02-22 2013-09-12 Caris Life Sciences Switzerland Holdings Gmbh Circulating biomarkers
WO2013018060A2 (en) * 2011-08-04 2013-02-07 Yeda Research And Development Co. Ltd. Micro-rnas and compositions comprising same for the treatment and diagnosis of serotonin-, adrenalin-, noradrenalin-, glutamate-, and corticotropin-releasing hormone- associated medical conditions
CN102628038A (zh) * 2012-04-09 2012-08-08 武汉生命之美科技有限公司 一种改进的clip法鉴定rna结合蛋白的靶标rna的方法
US20150301058A1 (en) * 2012-11-26 2015-10-22 Caris Science, Inc. Biomarker compositions and methods
US10435430B2 (en) * 2013-07-31 2019-10-08 Ionis Pharmaceuticals, Inc. Methods and compounds useful in conditions related to repeat expansion
CN105934439B (zh) 2013-11-27 2019-09-10 西格马-奥尔德里奇有限责任公司 从生物流体中分离微小rna
WO2016161375A2 (en) * 2015-04-03 2016-10-06 University Of Massachusetts Methods of using oligonucleotide-guided argonaute proteins

Also Published As

Publication number Publication date
CN110511924B (zh) 2023-11-03
KR102181103B1 (ko) 2020-11-23
CN110511924A (zh) 2019-11-29
EP3702361B1 (en) 2022-08-31
WO2015094609A1 (en) 2015-06-25
JP2017501689A (ja) 2017-01-19
US10711266B2 (en) 2020-07-14
CA2930704A1 (en) 2015-06-25
CN105934439A (zh) 2016-09-07
IL245646A0 (en) 2016-06-30
BR112016011770A2 (enExample) 2017-08-08
EP3074413B1 (en) 2020-01-22
IL245646B (en) 2018-06-28
EP3702361A1 (en) 2020-09-02
BR112016011770B1 (pt) 2022-10-25
US20160376582A1 (en) 2016-12-29
JP6644686B2 (ja) 2020-02-12
EP3074413A4 (en) 2017-05-31
US10131902B2 (en) 2018-11-20
EP3074413A1 (en) 2016-10-05
KR20160089398A (ko) 2016-07-27
CA2930704C (en) 2023-02-21
US20190144850A1 (en) 2019-05-16
ES2930344T3 (es) 2022-12-12
CN105934439B (zh) 2019-09-10

Similar Documents

Publication Publication Date Title
ES2778223T3 (es) Aislamiento de ARNmicro de fluido biológico
US9957548B2 (en) Methods of capturing sperm nucleic acids
CN103764827B (zh) 可用于分离和／或纯化核酸的试剂
CA2705267A1 (en) Method for isolation of genomic dna, rna and proteins from a single sample
WO2020006049A1 (en) Crispr/cas and transposase based amplification compositions, systems and methods
EP2620498B1 (en) Method for acquisition of small rna
JPWO2012002541A1 (ja) 標的分子の検出法
Kim et al. Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method
EP2216406B1 (en) Carrier, method and reagent for obtaining small rna
CN106168625B (zh) 一种RNA pulldown方法及试剂盒
KR20150044397A (ko) 테뷰코나졸, 메페나셋 및 이나벤파이드에 특이적으로 결합할 수 있는 핵산 압타머 및 그 용도
WO2017198715A1 (en) Nucleic acid stabilization agent
Hanazato et al. Selection of an aptamer against mouse GP2 by SELEX
WO2017026532A1 (ja) 抗原特異的モノクローナル抗体作製方法
Davydova et al. In vitro selection of cell-internalizing 2′-modified RNA aptamers against Pseudomonas aeruginosa
US20180327811A1 (en) Methods and kits for capturing sperm nucleic acids
RU2618872C1 (ru) Способ направленного истощения олигонуклеотидных библиотек для снижения неспецифической адсорбции при твердофазной селекции аптамеров на основе нуклеиновых кислот
KR20210152538A (ko) 기능성 risc-연합된 소형 rna의 정제 방법 및 키트
US20200325527A1 (en) Rna identity method using rnase h digestion and size fractionating
Gopinath et al. Generation of aptamer for biosensing applications
Khan SELECTION OF DNA APTAMERS TO HUMAN RED BLOOD CELLS FOR DRUG DELIVERY
CN118440942A (zh) 一种结合乙型流感病毒血凝素的核酸适配体及其筛选方法和应用
US20140073528A1 (en) Methods of depleting a target molecule in a sample and kits for practicing the same