CN110511924B - 从生物流体中分离微小rna - Google Patents

从生物流体中分离微小rna Download PDF

Info

Publication number
CN110511924B
CN110511924B CN201910746637.8A CN201910746637A CN110511924B CN 110511924 B CN110511924 B CN 110511924B CN 201910746637 A CN201910746637 A CN 201910746637A CN 110511924 B CN110511924 B CN 110511924B
Authority
CN
China
Prior art keywords
mirna
sodium
surfactant
plasma
rip
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910746637.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN110511924A (zh
Inventor
C.克里德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Sigma Aldrich Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co LLC filed Critical Sigma Aldrich Co LLC
Priority to CN201910746637.8A priority Critical patent/CN110511924B/zh
Publication of CN110511924A publication Critical patent/CN110511924A/zh
Application granted granted Critical
Publication of CN110511924B publication Critical patent/CN110511924B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21014—Microbial serine proteases (3.4.21.14)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201910746637.8A 2013-11-27 2014-11-25 从生物流体中分离微小rna Active CN110511924B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910746637.8A CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361909834P 2013-11-27 2013-11-27
US61/909834 2013-11-27
US201461985000P 2014-04-28 2014-04-28
US61/985000 2014-04-28
CN201480074125.7A CN105934439B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna
PCT/US2014/067321 WO2015094609A1 (en) 2013-11-27 2014-11-25 Micro rna isolation from biological fluid
CN201910746637.8A CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201480074125.7A Division CN105934439B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Publications (2)

Publication Number Publication Date
CN110511924A CN110511924A (zh) 2019-11-29
CN110511924B true CN110511924B (zh) 2023-11-03

Family

ID=53403495

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201480074125.7A Active CN105934439B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna
CN201910746637.8A Active CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201480074125.7A Active CN105934439B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Country Status (10)

Country Link
US (2) US10131902B2 (enExample)
EP (2) EP3074413B1 (enExample)
JP (1) JP6644686B2 (enExample)
KR (1) KR102181103B1 (enExample)
CN (2) CN105934439B (enExample)
BR (1) BR112016011770B1 (enExample)
CA (1) CA2930704C (enExample)
ES (2) ES2930344T3 (enExample)
IL (1) IL245646B (enExample)
WO (1) WO2015094609A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3074413B1 (en) 2013-11-27 2020-01-22 Sigma Aldrich Co. LLC Micro rna isolation from biological fluid
CN107385014B (zh) * 2017-06-13 2020-12-22 深圳大学 一种直接定量检测循环miRNA的RT-qPCR方法
KR102003594B1 (ko) 2017-08-08 2019-07-24 숙명여자대학교산학협력단 염증 반응 매개 신혈관생성 질환 진단용 조성물
CN111278844B (zh) 2017-09-13 2023-12-19 贝克顿·迪金森公司 用于使用氧化铁颗粒提取核酸的方法和组合物
JP7509687B2 (ja) * 2018-03-22 2024-07-02 アイデックス ラボラトリーズ インコーポレイテッド 生体試料中の細菌の測定方法
CN109490533B (zh) * 2018-12-29 2022-02-15 南京澳林生物科技有限公司 一种胃蛋白酶原ⅱ(pgⅱ)检测试剂盒及生产工艺
CA3199080A1 (en) * 2020-10-26 2022-05-05 Eclipse Bioinnovations, Inc. Methods and kits for enriching for polynucleotides
WO2023023584A2 (en) 2021-08-19 2023-02-23 Eclipse Bioinnovations, Inc. Methods for detecting rna binding protein complexes
CN113980954A (zh) * 2021-11-11 2022-01-28 德州学院 一种病毒rna保护剂及其制备方法与应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1867584A (zh) * 2003-10-08 2006-11-22 E生物科学公司 天然免疫球蛋白结合试剂及其制造和使用方法
CN102628038A (zh) * 2012-04-09 2012-08-08 武汉生命之美科技有限公司 一种改进的clip法鉴定rna结合蛋白的靶标rna的方法
CN103119163A (zh) * 2010-09-24 2013-05-22 和光纯药工业株式会社 小rna的获得方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5349838B2 (ja) * 2007-11-30 2013-11-20 和光純薬工業株式会社 ｓｍａｌｌＲＮＡの取得用担体、取得方法及び取得用試薬
WO2010014636A1 (en) * 2008-07-28 2010-02-04 Rockefeller University Methods for identifying rna segments bound by rna-binding proteins or ribonucleoprotein complexes
US8846314B2 (en) * 2009-03-03 2014-09-30 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoretic focusing of nucleic acids
CA2827894A1 (en) * 2011-02-22 2012-08-30 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Circulating biomarkers
CA2844012C (en) * 2011-08-04 2021-03-16 Yeda Research And Development Co. Ltd. Micro-rnas and compositions comprising same for the treatment and diagnosis of serotonin-, adrenalin-, noradrenalin-, glutamate-, and corticotropin-releasing hormone- associated medical conditions
CA2892490A1 (en) * 2012-11-26 2014-05-30 Caris Science, Inc. Biomarker compositions and methods
WO2015017675A2 (en) * 2013-07-31 2015-02-05 Isis Pharmaceuticals, Inc. Methods and compounds useful in conditions related to repeat expansion
EP3074413B1 (en) 2013-11-27 2020-01-22 Sigma Aldrich Co. LLC Micro rna isolation from biological fluid
WO2016161375A2 (en) * 2015-04-03 2016-10-06 University Of Massachusetts Methods of using oligonucleotide-guided argonaute proteins

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1867584A (zh) * 2003-10-08 2006-11-22 E生物科学公司 天然免疫球蛋白结合试剂及其制造和使用方法
CN103119163A (zh) * 2010-09-24 2013-05-22 和光纯药工业株式会社 小rna的获得方法
CN102628038A (zh) * 2012-04-09 2012-08-08 武汉生命之美科技有限公司 一种改进的clip法鉴定rna结合蛋白的靶标rna的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张均田.现代药理试验方法.《现代药理试验方法 上》.中国协和医科大学出版社,2012, *

Also Published As

Publication number Publication date
IL245646B (en) 2018-06-28
CA2930704A1 (en) 2015-06-25
WO2015094609A1 (en) 2015-06-25
EP3074413A1 (en) 2016-10-05
US10711266B2 (en) 2020-07-14
CN110511924A (zh) 2019-11-29
KR102181103B1 (ko) 2020-11-23
BR112016011770B1 (pt) 2022-10-25
US20190144850A1 (en) 2019-05-16
KR20160089398A (ko) 2016-07-27
JP2017501689A (ja) 2017-01-19
EP3702361A1 (en) 2020-09-02
IL245646A0 (en) 2016-06-30
US20160376582A1 (en) 2016-12-29
EP3074413A4 (en) 2017-05-31
CN105934439B (zh) 2019-09-10
BR112016011770A2 (enExample) 2017-08-08
CA2930704C (en) 2023-02-21
EP3074413B1 (en) 2020-01-22
ES2778223T3 (es) 2020-08-10
JP6644686B2 (ja) 2020-02-12
EP3702361B1 (en) 2022-08-31
ES2930344T3 (es) 2022-12-12
CN105934439A (zh) 2016-09-07
US10131902B2 (en) 2018-11-20

Similar Documents

Publication Publication Date Title
CN110511924B (zh) 从生物流体中分离微小rna
JP7573437B2 (ja) 膜タンパク質を使用する治療用エクソソームの調製
US11021736B2 (en) Rapid method for isolating extracellular nucleic acids
JP6689251B2 (ja) Ｒｎａを高収率で単離するための方法
CN107406892B (zh) 捕获精子核酸的方法
CA3101066A1 (en) Crispr/cas and transposase based amplification compositions, systems and methods
CN103764827A (zh) 可用于分离和／或纯化核酸的试剂
US20130197206A1 (en) Method for acquisition of small rna
US20240375024A1 (en) Methods of isolating a composition comprising a target molecule
CN113215234A (zh) 一种鉴定RNA结合蛋白靶位点的方法LACE-seq及试剂盒和应用
WO2017026532A1 (ja) 抗原特異的モノクローナル抗体作製方法
EP3665306A1 (en) Rna identity method using rnase h digestion and size fractionating
WO2024097756A1 (en) Interrupted ran proteins in disease

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant