CN110511924B - 从生物流体中分离微小rna - Google Patents

从生物流体中分离微小rna Download PDF

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CN110511924B
CN110511924B CN201910746637.8A CN201910746637A CN110511924B CN 110511924 B CN110511924 B CN 110511924B CN 201910746637 A CN201910746637 A CN 201910746637A CN 110511924 B CN110511924 B CN 110511924B
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mirna
sodium
mirnas
surfactant
ago
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CN110511924A (zh
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C.克里德
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Sigma Aldrich Co LLC
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Sigma Aldrich Co LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21014—Microbial serine proteases (3.4.21.14)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201910746637.8A 2013-11-27 2014-11-25 从生物流体中分离微小rna Active CN110511924B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910746637.8A CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361909834P 2013-11-27 2013-11-27
US61/909834 2013-11-27
US201461985000P 2014-04-28 2014-04-28
US61/985000 2014-04-28
CN201480074125.7A CN105934439B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna
PCT/US2014/067321 WO2015094609A1 (en) 2013-11-27 2014-11-25 Micro rna isolation from biological fluid
CN201910746637.8A CN110511924B (zh) 2013-11-27 2014-11-25 从生物流体中分离微小rna

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CN110511924A CN110511924A (zh) 2019-11-29
CN110511924B true CN110511924B (zh) 2023-11-03

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EP (2) EP3702361B1 (enExample)
JP (1) JP6644686B2 (enExample)
KR (1) KR102181103B1 (enExample)
CN (2) CN110511924B (enExample)
BR (1) BR112016011770B1 (enExample)
CA (1) CA2930704C (enExample)
ES (2) ES2778223T3 (enExample)
IL (1) IL245646B (enExample)
WO (1) WO2015094609A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
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BR112016011770B1 (pt) 2013-11-27 2022-10-25 Sigma-Aldrich Co. Llc Método e kit para isolamento de microrna a partir de amostra biológica
CN107385014B (zh) * 2017-06-13 2020-12-22 深圳大学 一种直接定量检测循环miRNA的RT-qPCR方法
KR102003594B1 (ko) 2017-08-08 2019-07-24 숙명여자대학교산학협력단 염증 반응 매개 신혈관생성 질환 진단용 조성물
EP3681895A4 (en) * 2017-09-13 2021-05-26 Becton, Dickinson and Company METHODS AND COMPOSITIONS FOR EXTRACTION OF NUCLEIC ACIDS USING FERROUS PARTICLES
AU2019238322B2 (en) * 2018-03-22 2025-08-14 Idexx Laboratories, Inc. Methods for measuring bacteria in biological samples
CN109490533B (zh) * 2018-12-29 2022-02-15 南京澳林生物科技有限公司 一种胃蛋白酶原ⅱ(pgⅱ)检测试剂盒及生产工艺
EP4232574A4 (en) * 2020-10-26 2024-12-18 Eclipse Bioinnovations, Inc. METHODS AND KITS FOR THE ENRICHMENT OF POLYNUCLEOTIDES
US11795500B2 (en) 2021-08-19 2023-10-24 Eclipse Bioinnovations, Inc. Methods for detecting RNA binding protein complexes
CN113980954A (zh) * 2021-11-11 2022-01-28 德州学院 一种病毒rna保护剂及其制备方法与应用

Citations (3)

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CN1867584A (zh) * 2003-10-08 2006-11-22 E生物科学公司 天然免疫球蛋白结合试剂及其制造和使用方法
CN102628038A (zh) * 2012-04-09 2012-08-08 武汉生命之美科技有限公司 一种改进的clip法鉴定rna结合蛋白的靶标rna的方法
CN103119163A (zh) * 2010-09-24 2013-05-22 和光纯药工业株式会社 小rna的获得方法

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US8841073B2 (en) * 2008-07-28 2014-09-23 The Rockefeller University Methods for identifying RNA segments bound by RNA-binding proteins or ribonucleoprotein complexes
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CN1867584A (zh) * 2003-10-08 2006-11-22 E生物科学公司 天然免疫球蛋白结合试剂及其制造和使用方法
CN103119163A (zh) * 2010-09-24 2013-05-22 和光纯药工业株式会社 小rna的获得方法
CN102628038A (zh) * 2012-04-09 2012-08-08 武汉生命之美科技有限公司 一种改进的clip法鉴定rna结合蛋白的靶标rna的方法

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Publication number Publication date
JP6644686B2 (ja) 2020-02-12
KR20160089398A (ko) 2016-07-27
EP3074413A1 (en) 2016-10-05
EP3702361B1 (en) 2022-08-31
CA2930704C (en) 2023-02-21
IL245646B (en) 2018-06-28
CN105934439A (zh) 2016-09-07
CN110511924A (zh) 2019-11-29
US20160376582A1 (en) 2016-12-29
US10711266B2 (en) 2020-07-14
ES2930344T3 (es) 2022-12-12
US20190144850A1 (en) 2019-05-16
CN105934439B (zh) 2019-09-10
KR102181103B1 (ko) 2020-11-23
EP3702361A1 (en) 2020-09-02
WO2015094609A1 (en) 2015-06-25
ES2778223T3 (es) 2020-08-10
IL245646A0 (en) 2016-06-30
JP2017501689A (ja) 2017-01-19
BR112016011770B1 (pt) 2022-10-25
CA2930704A1 (en) 2015-06-25
US10131902B2 (en) 2018-11-20
EP3074413A4 (en) 2017-05-31
EP3074413B1 (en) 2020-01-22
BR112016011770A2 (enExample) 2017-08-08

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